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The map label for this gene is phoB [H]

Identifier: 157960427

GI number: 157960427

Start: 735612

End: 736949

Strand: Reverse

Name: phoB [H]

Synonym: Spea_0598

Alternate gene names: 157960427

Gene position: 736949-735612 (Counterclockwise)

Preceding gene: 157960428

Following gene: 157960426

Centisome position: 14.24

GC content: 48.65

Gene sequence:

>1338_bases
ATGAACTTGAGAAAAACATGCACTTGGGCACTTAGTGCCCTTGTTGTGTTACCACTATTTGCATCGGCCAATATCGATAT
TCATGAGGCTCCATCGCGTCCTAAAAACATGATTATCATGATCGGTGATGGTATGGGACCGGCTTACACCAGCGCTTATC
GTTATTACCAAAATAACCCTGATACCGAAGAGATTGAGCAGACGGTTTTTGATAGGCTGCTAGTCGGCATGGCATCGACC
TACCCAGCTAGGCAGAGTGGCTATGTTACCGACTCAGCCGCATCGGCTACCGCTCTGGCAACTGGCGTTAAAAGTTATAA
TGGCGCCATCTCTGTCGATGTGGATAAGCGTCCGCTTACAACCATAATGCAGATGGCTAAAGCCCGCGGTATGGCAACCG
GTGTAGCCGTAACAGCTCAAGTTAATCATGCCACCCCAGCGGCTTTTTTAGCGCATAACGAAAGCCGTCGCAACTACGAT
GCAATCGCCCAAAGCTACTTAAGTTCTGGTGCTGACGTGATCCTAGGTGGTGGCCAAAAGTACTTCCCAGAAGATCTCCT
TAAGCAGTTTTCGGCTAAAGACTACCAACATATTAGCCAACTGAGTCAACTCGATAGTTTAACCCAAGGCAAGGCCTTGG
GCCTATTTGCCGAGGTGCAACTACCTTGGGTTATCGATCAGCAAGACTCGACTCAATTAAGCTTGCTGACCCAAAAAGCG
CTAGAGCTGTTATCGCAAAATCCAAATGGTTTTGTGCTGCTGGTTGAGGGCAGTCTGATCGATTGGGCCGGCCACAATAA
CGATATCGCCACCGCCATGGCTGAGATGCACGGTTTTGCCAATGCCATAGAAGTGGTCGAGCAATATGTACGCGCCAATC
CTGACACCTTGATGGTGGTGACCGCCGATCATAATACCGGTGGCTTATCGATTGGCGCTAATGGTGAGTATGCTTGGGAT
ACCGCGTTACTTAAAGGTATTCAAGCAAGCCCTGCGAGTATCGCAGAACTTGCTATCGCCATAGACGATTGGCAACCATT
AGTGAGTCAGAAGCTAGGTTTTGAACTTAAAGCCGAAGAACTACAGTCCCTCGGTAATGCCCGCATGCAAGGCCAAGTCG
CGTTAGATACCGCCTTAAAGCAGCTTATCGATAACCGCAGCTTTACCGGCTGGACTACTAGTGGTCACACTGGTGTCGAT
GTTCAGGTGTTTGCCGCTGGGCCTGCTGCCGATCTGTTTAAAGGTAATCAAGACAATACCGATATCGCAGACAAGATGAT
AAGCCTACTGCCTAGAGCCAAGCAAACTCTTGAAGTGATTTCGAAAGATAATAAGTGA

Upstream 100 bases:

>100_bases
TTAAAGAGATTGAAGCAGCCTTCGATGTCGATATCACAGGCATGTTAATGGATGCAATTGAACAGCGTATCAACAAATCA
GCATAGCCAAAGGATTCGGC

Downstream 100 bases:

>100_bases
CCACTAAACACGCGAAGAGCACGAAGGAAAGCCCCGATTTTTTACTTTTCTTCGAGCTCTCGGTGTAGCTAACAAAAACC
GCGCTTCGTGGTTATAGTTT

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MNLRKTCTWALSALVVLPLFASANIDIHEAPSRPKNMIIMIGDGMGPAYTSAYRYYQNNPDTEEIEQTVFDRLLVGMAST
YPARQSGYVTDSAASATALATGVKSYNGAISVDVDKRPLTTIMQMAKARGMATGVAVTAQVNHATPAAFLAHNESRRNYD
AIAQSYLSSGADVILGGGQKYFPEDLLKQFSAKDYQHISQLSQLDSLTQGKALGLFAEVQLPWVIDQQDSTQLSLLTQKA
LELLSQNPNGFVLLVEGSLIDWAGHNNDIATAMAEMHGFANAIEVVEQYVRANPDTLMVVTADHNTGGLSIGANGEYAWD
TALLKGIQASPASIAELAIAIDDWQPLVSQKLGFELKAEELQSLGNARMQGQVALDTALKQLIDNRSFTGWTTSGHTGVD
VQVFAAGPAADLFKGNQDNTDIADKMISLLPRAKQTLEVISKDNK

Sequences:

>Translated_445_residues
MNLRKTCTWALSALVVLPLFASANIDIHEAPSRPKNMIIMIGDGMGPAYTSAYRYYQNNPDTEEIEQTVFDRLLVGMAST
YPARQSGYVTDSAASATALATGVKSYNGAISVDVDKRPLTTIMQMAKARGMATGVAVTAQVNHATPAAFLAHNESRRNYD
AIAQSYLSSGADVILGGGQKYFPEDLLKQFSAKDYQHISQLSQLDSLTQGKALGLFAEVQLPWVIDQQDSTQLSLLTQKA
LELLSQNPNGFVLLVEGSLIDWAGHNNDIATAMAEMHGFANAIEVVEQYVRANPDTLMVVTADHNTGGLSIGANGEYAWD
TALLKGIQASPASIAELAIAIDDWQPLVSQKLGFELKAEELQSLGNARMQGQVALDTALKQLIDNRSFTGWTTSGHTGVD
VQVFAAGPAADLFKGNQDNTDIADKMISLLPRAKQTLEVISKDNK
>Mature_445_residues
MNLRKTCTWALSALVVLPLFASANIDIHEAPSRPKNMIIMIGDGMGPAYTSAYRYYQNNPDTEEIEQTVFDRLLVGMAST
YPARQSGYVTDSAASATALATGVKSYNGAISVDVDKRPLTTIMQMAKARGMATGVAVTAQVNHATPAAFLAHNESRRNYD
AIAQSYLSSGADVILGGGQKYFPEDLLKQFSAKDYQHISQLSQLDSLTQGKALGLFAEVQLPWVIDQQDSTQLSLLTQKA
LELLSQNPNGFVLLVEGSLIDWAGHNNDIATAMAEMHGFANAIEVVEQYVRANPDTLMVVTADHNTGGLSIGANGEYAWD
TALLKGIQASPASIAELAIAIDDWQPLVSQKLGFELKAEELQSLGNARMQGQVALDTALKQLIDNRSFTGWTTSGHTGVD
VQVFAAGPAADLFKGNQDNTDIADKMISLLPRAKQTLEVISKDNK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=480, Percent_Identity=30, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI94721246, Length=460, Percent_Identity=30, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI157266292, Length=454, Percent_Identity=29.7356828193833, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI116734717, Length=460, Percent_Identity=27.8260869565217, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI294660770, Length=456, Percent_Identity=27.4122807017544, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI294660772, Length=401, Percent_Identity=27.431421446384, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI48994877, Length=334, Percent_Identity=35.9281437125749, Blast_Score=159, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6320689, Length=482, Percent_Identity=30.0829875518672, Blast_Score=172, Evalue=9e-44,
Organism=Drosophila melanogaster, GI21355981, Length=449, Percent_Identity=26.9487750556793, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21355149, Length=459, Percent_Identity=26.3616557734205, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21355151, Length=455, Percent_Identity=25.9340659340659, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI85725200, Length=440, Percent_Identity=27.7272727272727, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI85815833, Length=440, Percent_Identity=27.7272727272727, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24651554, Length=465, Percent_Identity=28.1720430107527, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18859923, Length=413, Percent_Identity=27.1186440677966, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21358067, Length=332, Percent_Identity=28.6144578313253, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24657842, Length=445, Percent_Identity=25.3932584269663, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24657827, Length=445, Percent_Identity=25.6179775280899, Blast_Score=82, Evalue=9e-16,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 47943; Mature: 47943

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRKTCTWALSALVVLPLFASANIDIHEAPSRPKNMIIMIGDGMGPAYTSAYRYYQNNP
CCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEECCCCHHHHHHHHHHCCCC
DTEEIEQTVFDRLLVGMASTYPARQSGYVTDSAASATALATGVKSYNGAISVDVDKRPLT
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCHHH
TIMQMAKARGMATGVAVTAQVNHATPAAFLAHNESRRNYDAIAQSYLSSGADVILGGGQK
HHHHHHHHCCCCCCEEEEEEECCCCCHHHEECCCCCCHHHHHHHHHHHCCCCEEEECCCC
YFPEDLLKQFSAKDYQHISQLSQLDSLTQGKALGLFAEVQLPWVIDQQDSTQLSLLTQKA
CCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCEEECCCCCHHHHHHHHHH
LELLSQNPNGFVLLVEGSLIDWAGHNNDIATAMAEMHGFANAIEVVEQYVRANPDTLMVV
HHHHHCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
TADHNTGGLSIGANGEYAWDTALLKGIQASPASIAELAIAIDDWQPLVSQKLGFELKAEE
EECCCCCCEEECCCCCCHHHHHHHHCCCCCCHHHHHHEEEECCCHHHHHHHCCCEECHHH
LQSLGNARMQGQVALDTALKQLIDNRSFTGWTTSGHTGVDVQVFAAGPAADLFKGNQDNT
HHHHCCCHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCH
DIADKMISLLPRAKQTLEVISKDNK
HHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNLRKTCTWALSALVVLPLFASANIDIHEAPSRPKNMIIMIGDGMGPAYTSAYRYYQNNP
CCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEECCCCHHHHHHHHHHCCCC
DTEEIEQTVFDRLLVGMASTYPARQSGYVTDSAASATALATGVKSYNGAISVDVDKRPLT
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCHHH
TIMQMAKARGMATGVAVTAQVNHATPAAFLAHNESRRNYDAIAQSYLSSGADVILGGGQK
HHHHHHHHCCCCCCEEEEEEECCCCCHHHEECCCCCCHHHHHHHHHHHCCCCEEEECCCC
YFPEDLLKQFSAKDYQHISQLSQLDSLTQGKALGLFAEVQLPWVIDQQDSTQLSLLTQKA
CCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCEEECCCCCHHHHHHHHHH
LELLSQNPNGFVLLVEGSLIDWAGHNNDIATAMAEMHGFANAIEVVEQYVRANPDTLMVV
HHHHHCCCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
TADHNTGGLSIGANGEYAWDTALLKGIQASPASIAELAIAIDDWQPLVSQKLGFELKAEE
EECCCCCCEEECCCCCCHHHHHHHHCCCCCCHHHHHHEEEECCCHHHHHHHCCCEECHHH
LQSLGNARMQGQVALDTALKQLIDNRSFTGWTTSGHTGVDVQVFAAGPAADLFKGNQDNT
HHHHCCCHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCH
DIADKMISLLPRAKQTLEVISKDNK
HHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301 [H]