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The map label for this gene is yciL [C]

Identifier: 157825866

GI number: 157825866

Start: 723529

End: 724218

Strand: Direct

Name: yciL [C]

Synonym: A1C_04025

Alternate gene names: 157825866

Gene position: 723529-724218 (Clockwise)

Preceding gene: 157825863

Following gene: 157825867

Centisome position: 58.77

GC content: 30.87

Gene sequence:

>690_bases
ATGCAAAGATTAGCCAAAATAATTAGTAATGCAGGAATATGTTCTAGGCGTGATGCTGAAAAGCTGATAGCTGAAGGACA
GGTAAAAGTAGACGGTATTACAATTTTATCACCCGCTACAAATGTTGATATTAGTAATAAAATCGAAGTATCAGGCATAT
TAATCAATAATATACAAAAACCAAGGCTTTGGATTTATTATAAGCCTGTGGGTTTAATTACTACTCATAAAGATCCATTA
TCTCGTAAAACGGTTTTTGAACAACTAACAGGTTTACCTCGTGTTATTTCAATAGGTAGGCTGGATTTAAATAGCGAAGG
TTTGTTATTACTAACTAATAGCGGTGACTTAGCTCGTCAGTTTGAGCTACCTTCAAATAGGTTAAAGCGAGTATATCATG
TTAGGGCATATGGGAAGTCTGATATTCTACTAAAAAGCGATTATAAAAATTTAGAAATTGACGGGATATTTTATAATCCT
CATTCGATAAAGTTACTTAAGCAAAATAAAAGTAATTCTTGGTTTGAAGTAGTTTTATTTGAAGGAAAAAATCGTGAAAT
TAGAAGAATATTCGAGTATTTTGGACTAAAAGTGCACAAATTAATTAGGATTCAATATGGTAGTTTTACAATAGATAATC
TAAAACCCGGAGCTTATAAAGAAATAAATAATAAAATACTGGCAGAATAA

Upstream 100 bases:

>100_bases
GCTTTTGAATGGTATTTTTGTATGTTTTTATTAGACATCTTTTAAATAACCTCTAGAAGCCGGTTTAGAAAGATGTCTAT
GAAATTTACTATATTTAAAA

Downstream 100 bases:

>100_bases
TTAGTAATGTGTTACCATTAACGTATTTTAACTAATCATATAGAAAACATATGACAAATCTTAATATATATAAATGTGAA
GAAAAAGACTTAAACGATTA

Product: ribosomal large subunit pseudouridine synthase

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MQRLAKIISNAGICSRRDAEKLIAEGQVKVDGITILSPATNVDISNKIEVSGILINNIQKPRLWIYYKPVGLITTHKDPL
SRKTVFEQLTGLPRVISIGRLDLNSEGLLLLTNSGDLARQFELPSNRLKRVYHVRAYGKSDILLKSDYKNLEIDGIFYNP
HSIKLLKQNKSNSWFEVVLFEGKNREIRRIFEYFGLKVHKLIRIQYGSFTIDNLKPGAYKEINNKILAE

Sequences:

>Translated_229_residues
MQRLAKIISNAGICSRRDAEKLIAEGQVKVDGITILSPATNVDISNKIEVSGILINNIQKPRLWIYYKPVGLITTHKDPL
SRKTVFEQLTGLPRVISIGRLDLNSEGLLLLTNSGDLARQFELPSNRLKRVYHVRAYGKSDILLKSDYKNLEIDGIFYNP
HSIKLLKQNKSNSWFEVVLFEGKNREIRRIFEYFGLKVHKLIRIQYGSFTIDNLKPGAYKEINNKILAE
>Mature_229_residues
MQRLAKIISNAGICSRRDAEKLIAEGQVKVDGITILSPATNVDISNKIEVSGILINNIQKPRLWIYYKPVGLITTHKDPL
SRKTVFEQLTGLPRVISIGRLDLNSEGLLLLTNSGDLARQFELPSNRLKRVYHVRAYGKSDILLKSDYKNLEIDGIFYNP
HSIKLLKQNKSNSWFEVVLFEGKNREIRRIFEYFGLKVHKLIRIQYGSFTIDNLKPGAYKEINNKILAE

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=231, Percent_Identity=37.2294372294372, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1790453, Length=230, Percent_Identity=30, Blast_Score=88, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 26181; Mature: 26181

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRLAKIISNAGICSRRDAEKLIAEGQVKVDGITILSPATNVDISNKIEVSGILINNIQK
CHHHHHHHHCCCCCCCCCHHHHHHCCCEEECCEEEECCCCCCCCCCEEEEEEEEECCCCC
PRLWIYYKPVGLITTHKDPLSRKTVFEQLTGLPRVISIGRLDLNSEGLLLLTNSGDLARQ
CEEEEEEECEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEECCCCCEEE
FELPSNRLKRVYHVRAYGKSDILLKSDYKNLEIDGIFYNPHSIKLLKQNKSNSWFEVVLF
ECCCHHHHHHEEEEEECCCCCEEEECCCCCEEEEEEEECCCEEEEEEECCCCCEEEEEEE
EGKNREIRRIFEYFGLKVHKLIRIQYGSFTIDNLKPGAYKEINNKILAE
ECCCHHHHHHHHHHCCEEEEEEEEEECCEEECCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MQRLAKIISNAGICSRRDAEKLIAEGQVKVDGITILSPATNVDISNKIEVSGILINNIQK
CHHHHHHHHCCCCCCCCCHHHHHHCCCEEECCEEEECCCCCCCCCCEEEEEEEEECCCCC
PRLWIYYKPVGLITTHKDPLSRKTVFEQLTGLPRVISIGRLDLNSEGLLLLTNSGDLARQ
CEEEEEEECEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEECCCCCEEE
FELPSNRLKRVYHVRAYGKSDILLKSDYKNLEIDGIFYNPHSIKLLKQNKSNSWFEVVLF
ECCCHHHHHHEEEEEECCCCCEEEECCCCCEEEEEEEECCCEEEEEEECCCCCEEEEEEE
EGKNREIRRIFEYFGLKVHKLIRIQYGSFTIDNLKPGAYKEINNKILAE
ECCCHHHHHHHHHHCCEEEEEEEEEECCEEECCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]