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The map label for this gene is pheS [H]

Identifier: 157825702

GI number: 157825702

Start: 568674

End: 569723

Strand: Direct

Name: pheS [H]

Synonym: A1C_03140

Alternate gene names: 157825702

Gene position: 568674-569723 (Clockwise)

Preceding gene: 157825701

Following gene: 157825703

Centisome position: 46.19

GC content: 34.0

Gene sequence:

>1050_bases
ATGGAAAATATAGAAACAATATTAAGGCTTGCTGAAGAAAAAATCTTATTAGTACAAAACTTAAAAGCACTACAAGAATA
TAAAGTAGAATTTTTAGGTAAAAACGGTATAGTGACCGGTGAGCTTAAAAAACTAGGTAGTTTAAATGAACAGGAACGTA
AAGAATTTGGTTTAAAAATCAATAAATTAAAAGATAAAATACAGAATATAATAAGAGCAACAGAAGAAATTTTAGAGGAG
CAGGAACTAAAGTTAAAACTTGCTGCAGATAAAATTGATTTAACAATCCCTGCAAGGAGATATAAACAAGGTTCTATTCA
TCCAATAACACAATGTAGTGAGGAGTTAATACAAGTATTTTCGCAGTTTGGTTTTACTATAGAAAACGGACCAAATATCG
AGAATGATTTCCATAATTTTACTGCTCTCAATTTTGAAGATGACCATCCCGCAAGGCAAATGCATGATACTTTTTATTTG
AAAGGTCAAGAAAATAATAAACCGCTGTTATTACGTACTCATACCTCAACAGTGCAGATTAGAGCGATGAAAAGCGGCAA
ACCGCCTTTTAGGTTTATAGCACCTGGTAGGACTTATAGATCAGATTCGGATATGACGCATACGCCGATGTTTCACCAAA
TAGAAGGACTTGTTATTGATAAAAATATCAATATGGGGCATTTGAAATATGTTATCACAGAATTTATAAAAAACTTTTTT
GAAAATTCTAATATTGAATTACGTTTTAGACCTAGCTTCTTCCCATTTACCGAACCTTCTGCCGAAGTTGATATTCGGAT
GAATAAACACGATAAATGGCTTGAGGTCCTCGGTTGCGGGATGGTTCATCCAAACGTGCTTAAAAATGTCGGTATCGATA
GCAGCGAGTATCAAGGTGTTGCTTTTGGGCTGGGGGTAGAGCGTTTTGCAATGCTAAAATATAATATCAAAGATTTAAGA
CAATTTTTTGAAGGAGATATGCGTTGGCTTAAACATTATAATTTTGGGAGCTTTGATATACCGAGTTTAGCAGGAGGGCT
GACGAAATGA

Upstream 100 bases:

>100_bases
TATGTCACCACGTGGTTTTGACCACGATATCCAGACAATTTTCAAAAAAACTGGACCCCGTGGTCAAGCCATTGGGTGAC
AAAACAAAATAAAAAAAATC

Downstream 100 bases:

>100_bases
AATTTACGTTATCATGGTTAAAGCAATTGTTAGAGACATCGGCTTCAGTTACTGAAATTGCCGAAGCCCTAACAGCTATT
GGTCTTGAAGTAGAAGAGGT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MENIETILRLAEEKILLVQNLKALQEYKVEFLGKNGIVTGELKKLGSLNEQERKEFGLKINKLKDKIQNIIRATEEILEE
QELKLKLAADKIDLTIPARRYKQGSIHPITQCSEELIQVFSQFGFTIENGPNIENDFHNFTALNFEDDHPARQMHDTFYL
KGQENNKPLLLRTHTSTVQIRAMKSGKPPFRFIAPGRTYRSDSDMTHTPMFHQIEGLVIDKNINMGHLKYVITEFIKNFF
ENSNIELRFRPSFFPFTEPSAEVDIRMNKHDKWLEVLGCGMVHPNVLKNVGIDSSEYQGVAFGLGVERFAMLKYNIKDLR
QFFEGDMRWLKHYNFGSFDIPSLAGGLTK

Sequences:

>Translated_349_residues
MENIETILRLAEEKILLVQNLKALQEYKVEFLGKNGIVTGELKKLGSLNEQERKEFGLKINKLKDKIQNIIRATEEILEE
QELKLKLAADKIDLTIPARRYKQGSIHPITQCSEELIQVFSQFGFTIENGPNIENDFHNFTALNFEDDHPARQMHDTFYL
KGQENNKPLLLRTHTSTVQIRAMKSGKPPFRFIAPGRTYRSDSDMTHTPMFHQIEGLVIDKNINMGHLKYVITEFIKNFF
ENSNIELRFRPSFFPFTEPSAEVDIRMNKHDKWLEVLGCGMVHPNVLKNVGIDSSEYQGVAFGLGVERFAMLKYNIKDLR
QFFEGDMRWLKHYNFGSFDIPSLAGGLTK
>Mature_349_residues
MENIETILRLAEEKILLVQNLKALQEYKVEFLGKNGIVTGELKKLGSLNEQERKEFGLKINKLKDKIQNIIRATEEILEE
QELKLKLAADKIDLTIPARRYKQGSIHPITQCSEELIQVFSQFGFTIENGPNIENDFHNFTALNFEDDHPARQMHDTFYL
KGQENNKPLLLRTHTSTVQIRAMKSGKPPFRFIAPGRTYRSDSDMTHTPMFHQIEGLVIDKNINMGHLKYVITEFIKNFF
ENSNIELRFRPSFFPFTEPSAEVDIRMNKHDKWLEVLGCGMVHPNVLKNVGIDSSEYQGVAFGLGVERFAMLKYNIKDLR
QFFEGDMRWLKHYNFGSFDIPSLAGGLTK

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=260, Percent_Identity=33.8461538461538, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI5729820, Length=256, Percent_Identity=31.25, Blast_Score=115, Evalue=7e-26,
Organism=Escherichia coli, GI1788007, Length=333, Percent_Identity=49.5495495495495, Blast_Score=350, Evalue=7e-98,
Organism=Caenorhabditis elegans, GI17508957, Length=260, Percent_Identity=30.7692307692308, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI32563657, Length=260, Percent_Identity=30.7692307692308, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI32566635, Length=286, Percent_Identity=31.8181818181818, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6321087, Length=261, Percent_Identity=31.0344827586207, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6325304, Length=282, Percent_Identity=28.0141843971631, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI18858079, Length=282, Percent_Identity=28.3687943262411, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI17137424, Length=245, Percent_Identity=29.3877551020408, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 40374; Mature: 40374

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENIETILRLAEEKILLVQNLKALQEYKVEFLGKNGIVTGELKKLGSLNEQERKEFGLKI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCHHHHHHHCCCH
NKLKDKIQNIIRATEEILEEQELKLKLAADKIDLTIPARRYKQGSIHPITQCSEELIQVF
HHHHHHHHHHHHHHHHHHHHHHHEEEEEECEEEEEECHHHCCCCCCCHHHHHHHHHHHHH
SQFGFTIENGPNIENDFHNFTALNFEDDHPARQMHDTFYLKGQENNKPLLLRTHTSTVQI
HHCCCEECCCCCCCCCCCCEEEECCCCCCCHHHHCCEEEEECCCCCCCEEEEECCCEEEE
RAMKSGKPPFRFIAPGRTYRSDSDMTHTPMFHQIEGLVIDKNINMGHLKYVITEFIKNFF
EEECCCCCCEEEECCCCCCCCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHH
ENSNIELRFRPSFFPFTEPSAEVDIRMNKHDKWLEVLGCGMVHPNVLKNVGIDSSEYQGV
CCCCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHCCCCCCCCCCE
AFGLGVERFAMLKYNIKDLRQFFEGDMRWLKHYNFGSFDIPSLAGGLTK
EEECCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCC
>Mature Secondary Structure
MENIETILRLAEEKILLVQNLKALQEYKVEFLGKNGIVTGELKKLGSLNEQERKEFGLKI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCHHHHHHHCCCH
NKLKDKIQNIIRATEEILEEQELKLKLAADKIDLTIPARRYKQGSIHPITQCSEELIQVF
HHHHHHHHHHHHHHHHHHHHHHHEEEEEECEEEEEECHHHCCCCCCCHHHHHHHHHHHHH
SQFGFTIENGPNIENDFHNFTALNFEDDHPARQMHDTFYLKGQENNKPLLLRTHTSTVQI
HHCCCEECCCCCCCCCCCCEEEECCCCCCCHHHHCCEEEEECCCCCCCEEEEECCCEEEE
RAMKSGKPPFRFIAPGRTYRSDSDMTHTPMFHQIEGLVIDKNINMGHLKYVITEFIKNFF
EEECCCCCCEEEECCCCCCCCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHH
ENSNIELRFRPSFFPFTEPSAEVDIRMNKHDKWLEVLGCGMVHPNVLKNVGIDSSEYQGV
CCCCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHCCCCCCCCCCE
AFGLGVERFAMLKYNIKDLRQFFEGDMRWLKHYNFGSFDIPSLAGGLTK
EEECCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA