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The map label for this gene is fabI [H]

Identifier: 157825627

GI number: 157825627

Start: 490076

End: 490858

Strand: Reverse

Name: fabI [H]

Synonym: A1C_02720

Alternate gene names: 157825627

Gene position: 490858-490076 (Counterclockwise)

Preceding gene: 157825632

Following gene: 157825620

Centisome position: 39.87

GC content: 35.12

Gene sequence:

>783_bases
ATGACTACAGGATTACTACAAGGAAAAAAAGGCTTAATTACAGGCATTGCAAATAATATGTCAATATCGTGGGCGATTGC
ACAGCTTACTAAAAAACACGGAGCAGAGCTGTGGTTTACGTACCAATCGGAAGTACTAGAAAAACGAGTTAAACCACTCG
CTGAGGAAATAGGTTGTAATTTTGTTAGCGAACTTGATGTTACGAATCCAAAATCAATTAGTAATTTATTTGATGATATA
AAAGCAAAATGGTATAGCTTCGATTTCTTACTGCACGGTATGGCTTTTGCCGATAAAAATGAATTAAAAGGACGTTATGT
TGATACTAGCTTAGAAAATTTTCATAATTCTTTACATATATCATGTTACTCTCTTTTAGAACTCTCACGGTCTGCAGAAG
CCTTAATGCATGACGGCGGAAGCATTGTGACGTTAACCTACTACGGTGCTGAAAAAGTTATACCTAATTATAATGTAATG
GGGATTTCTAAAGCTGCACTTGAGGCTAGTGTAAAATATCTAGCAAACGACTTGGGAAAAAATAATATTAGAGTAAATGC
TATTTCGGCAGGACCTATCAAAACTCTAGCATCTAGTGCAATAGGTGATTTTAGTACTATGCTTAAATCTCACGCTACAA
ACGCACCGTTAAAACGTAATACTACCCAAGAAGATGTAGGAGGAGCAGCAGTATATTTATTTAGTAATTTATCTCAAGGT
GTTACCGGTGAAATTCATTATGTAGATTGCGGTTACAATATTATGGGAAGTCATAAATTATAA

Upstream 100 bases:

>100_bases
GATTTTAGATTTATACATTTTTATCGATTGTATAATATTTGTTTACAAAATAATACATCATGCTATCATTTCAGCAAGTT
TTAATATAATGAGTAAATAA

Downstream 100 bases:

>100_bases
ATTATCATTGCATATATCATTAAACGCGTTCGATGTCATACCAGCGTGGCGTTGTTGCATGGACCGGTAAAGGTTCTTTA
TGTCATTCTTAGCTAAAAGC

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTTGLLQGKKGLITGIANNMSISWAIAQLTKKHGAELWFTYQSEVLEKRVKPLAEEIGCNFVSELDVTNPKSISNLFDDI
KAKWYSFDFLLHGMAFADKNELKGRYVDTSLENFHNSLHISCYSLLELSRSAEALMHDGGSIVTLTYYGAEKVIPNYNVM
GISKAALEASVKYLANDLGKNNIRVNAISAGPIKTLASSAIGDFSTMLKSHATNAPLKRNTTQEDVGGAAVYLFSNLSQG
VTGEIHYVDCGYNIMGSHKL

Sequences:

>Translated_260_residues
MTTGLLQGKKGLITGIANNMSISWAIAQLTKKHGAELWFTYQSEVLEKRVKPLAEEIGCNFVSELDVTNPKSISNLFDDI
KAKWYSFDFLLHGMAFADKNELKGRYVDTSLENFHNSLHISCYSLLELSRSAEALMHDGGSIVTLTYYGAEKVIPNYNVM
GISKAALEASVKYLANDLGKNNIRVNAISAGPIKTLASSAIGDFSTMLKSHATNAPLKRNTTQEDVGGAAVYLFSNLSQG
VTGEIHYVDCGYNIMGSHKL
>Mature_259_residues
TTGLLQGKKGLITGIANNMSISWAIAQLTKKHGAELWFTYQSEVLEKRVKPLAEEIGCNFVSELDVTNPKSISNLFDDIK
AKWYSFDFLLHGMAFADKNELKGRYVDTSLENFHNSLHISCYSLLELSRSAEALMHDGGSIVTLTYYGAEKVIPNYNVMG
ISKAALEASVKYLANDLGKNNIRVNAISAGPIKTLASSAIGDFSTMLKSHATNAPLKRNTTQEDVGGAAVYLFSNLSQGV
TGEIHYVDCGYNIMGSHKL

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=258, Percent_Identity=43.4108527131783, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI1789378, Length=257, Percent_Identity=26.8482490272374, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082100, Length=265, Percent_Identity=27.5471698113208, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI2367365, Length=254, Percent_Identity=27.1653543307087, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787905, Length=255, Percent_Identity=27.4509803921569, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28411; Mature: 28279

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTGLLQGKKGLITGIANNMSISWAIAQLTKKHGAELWFTYQSEVLEKRVKPLAEEIGCN
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCC
FVSELDVTNPKSISNLFDDIKAKWYSFDFLLHGMAFADKNELKGRYVDTSLENFHNSLHI
CCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEECCHHHHHCCHHHH
SCYSLLELSRSAEALMHDGGSIVTLTYYGAEKVIPNYNVMGISKAALEASVKYLANDLGK
HHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHCCCCCCEEECHHHHHHHHHHHHHHHCCC
NNIRVNAISAGPIKTLASSAIGDFSTMLKSHATNAPLKRNTTQEDVGGAAVYLFSNLSQG
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEEEHHCCCC
VTGEIHYVDCGYNIMGSHKL
CCCEEEEEECCCEECCCCCC
>Mature Secondary Structure 
TTGLLQGKKGLITGIANNMSISWAIAQLTKKHGAELWFTYQSEVLEKRVKPLAEEIGCN
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCC
FVSELDVTNPKSISNLFDDIKAKWYSFDFLLHGMAFADKNELKGRYVDTSLENFHNSLHI
CCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEECCHHHHHCCHHHH
SCYSLLELSRSAEALMHDGGSIVTLTYYGAEKVIPNYNVMGISKAALEASVKYLANDLGK
HHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHCCCCCCEEECHHHHHHHHHHHHHHHCCC
NNIRVNAISAGPIKTLASSAIGDFSTMLKSHATNAPLKRNTTQEDVGGAAVYLFSNLSQG
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEEEHHCCCC
VTGEIHYVDCGYNIMGSHKL
CCCEEEEEECCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]