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The map label for this gene is wecB [C]

Identifier: 157825585

GI number: 157825585

Start: 454345

End: 455604

Strand: Direct

Name: wecB [C]

Synonym: A1C_02490

Alternate gene names: 157825585

Gene position: 454345-455604 (Clockwise)

Preceding gene: 157825584

Following gene: 157825586

Centisome position: 36.91

GC content: 36.43

Gene sequence:

>1260_bases
ATGCTTAAGGTAATGACTATTGTCGGTACTCGTCCTGAACTGATTAAACTATGCTGCGTGATTTCCGAGTTTGATAAATA
TACTAATCATATCCTAGTTCACACCGGTCAAAATTATGCATATGAATTAAATCAGGTGTTTTTTGATGATATGGGTATTA
GAAAACCCGATTATTTCTTAGAAGTAGCAGCAGATAATACAGCAAAATCTATTGGGCTTATTATCGAACAAGTTGACGCA
GTCCTTGAGAAAGAAAAGCCCGATGCCGTTTTGTTTTACGGTGATACTAATTCCTGCTTATCGGCAATTGCTGCTAAACG
TCGTAAAATTCCTATTTTCCATATGGAAGCAGGAAATCGTTGTTTTGATCAAAGAGTACCTGAAGAAATAAACCGTAAAA
TCATTGATCATATTAGTGATGTGAACATTACGTTAACCGAGCATGCAAGACGTTATTTAATTTCTGAAGGTTTACCGCCT
GAGCTTATTTTTAAATCAGGCTCGCATATGCCTGAAGTTCTTGACCGTTTTATGCCTAAAATATTAAAGTCGGATATTTT
AGATACATTATCATTACAAGCAAAGCAGTATTTCTTAATTAGCTCACATCGTGAGGAAAATGTTGACGTTAAAAATAATC
TGCAAGAATTATTAAGTAGTTTACAAACGCTTATTAAAGAGTATAATTTCCCCGTAATCTTCTCAACACACCCAAGGACT
AAAAAAAGACTTGAGGATTTAGGTGGTGGGAAAGAGCTTGGTGATAAAATAAGGTTTTTACCGGCTTTTAGTTTTACGGA
TTACGTCAAACTTCAGATGAATGCGTTTTGTATTTTATCCGATAGCGGTACTATTACTGAAGAAGCCTCTATACTTAATT
TACCTGCTTTAAACATTCGAGAAGCACATGAGCGTCCTGAAGGTATGGACGCCGGAACGCTTATTATGTCGGGCTTTAAT
GCTGCACGGGTACTACAATCGGTGAAATCAATTACGGCAGAACACGAGAATAATAAAGATACGGAAGAAATATACTCAAA
TGATTTGGAGAATTGTAATGTAAAACGAGGAACGAGCACGCACAGCCTATACTTAATAGGTGGGCATGCGAGTTCTCCAA
AGTTTTGCAGAGATAATTCTTCAAAGCAGAAGAGTATTGTGTCGGATTATGCTGCAGCAGGACTTGTATCAAAGAAAATA
TTGCGTATAGTTTTAAGTTATGTGGATTATGTTAACCGTACGGTGTGGTTTAAGAAGTAA

Upstream 100 bases:

>100_bases
CTTTTAGAGGATTACACTTCGCATAATACAAAACGTTTAAATTTAGAAGAAGTAAAAAAGTTGTTGCTGACTCTTGATTA
CATACAAGAGGAGCTAAAAA

Downstream 100 bases:

>100_bases
CATTATTCATTGTTATCATATCAACAACATGAAATTCTTTAAATTAATAAAACTTAACCTTGTATTTTTAAGTGTTTTTT
TACTAATAACTCTTATATCT

Product: UDP-N-acetylglucosamine 2-epimerase

Products: UDP-N-acetyl-D-mannosamine

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MLKVMTIVGTRPELIKLCCVISEFDKYTNHILVHTGQNYAYELNQVFFDDMGIRKPDYFLEVAADNTAKSIGLIIEQVDA
VLEKEKPDAVLFYGDTNSCLSAIAAKRRKIPIFHMEAGNRCFDQRVPEEINRKIIDHISDVNITLTEHARRYLISEGLPP
ELIFKSGSHMPEVLDRFMPKILKSDILDTLSLQAKQYFLISSHREENVDVKNNLQELLSSLQTLIKEYNFPVIFSTHPRT
KKRLEDLGGGKELGDKIRFLPAFSFTDYVKLQMNAFCILSDSGTITEEASILNLPALNIREAHERPEGMDAGTLIMSGFN
AARVLQSVKSITAEHENNKDTEEIYSNDLENCNVKRGTSTHSLYLIGGHASSPKFCRDNSSKQKSIVSDYAAAGLVSKKI
LRIVLSYVDYVNRTVWFKK

Sequences:

>Translated_419_residues
MLKVMTIVGTRPELIKLCCVISEFDKYTNHILVHTGQNYAYELNQVFFDDMGIRKPDYFLEVAADNTAKSIGLIIEQVDA
VLEKEKPDAVLFYGDTNSCLSAIAAKRRKIPIFHMEAGNRCFDQRVPEEINRKIIDHISDVNITLTEHARRYLISEGLPP
ELIFKSGSHMPEVLDRFMPKILKSDILDTLSLQAKQYFLISSHREENVDVKNNLQELLSSLQTLIKEYNFPVIFSTHPRT
KKRLEDLGGGKELGDKIRFLPAFSFTDYVKLQMNAFCILSDSGTITEEASILNLPALNIREAHERPEGMDAGTLIMSGFN
AARVLQSVKSITAEHENNKDTEEIYSNDLENCNVKRGTSTHSLYLIGGHASSPKFCRDNSSKQKSIVSDYAAAGLVSKKI
LRIVLSYVDYVNRTVWFKK
>Mature_419_residues
MLKVMTIVGTRPELIKLCCVISEFDKYTNHILVHTGQNYAYELNQVFFDDMGIRKPDYFLEVAADNTAKSIGLIIEQVDA
VLEKEKPDAVLFYGDTNSCLSAIAAKRRKIPIFHMEAGNRCFDQRVPEEINRKIIDHISDVNITLTEHARRYLISEGLPP
ELIFKSGSHMPEVLDRFMPKILKSDILDTLSLQAKQYFLISSHREENVDVKNNLQELLSSLQTLIKEYNFPVIFSTHPRT
KKRLEDLGGGKELGDKIRFLPAFSFTDYVKLQMNAFCILSDSGTITEEASILNLPALNIREAHERPEGMDAGTLIMSGFN
AARVLQSVKSITAEHENNKDTEEIYSNDLENCNVKRGTSTHSLYLIGGHASSPKFCRDNSSKQKSIVSDYAAAGLVSKKI
LRIVLSYVDYVNRTVWFKK

Specific function: Catalyzes The Reversible Epimerization At C-2 Of UDP-N- Acetylglucosamine (UDP-Glcnac) And Thereby Provides Bacteria With UDP-N-Acetylmannosamine (UDP-Mannac), The Activated Donor Of Mannac Residues. Also Involved In Bacteriophage N4 Adsorption. [C]

COG id: COG0381

COG function: function code M; UDP-N-acetylglucosamine 2-epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-N-acetylglucosamine 2-epimerase family [H]

Homologues:

Organism=Escherichia coli, GI48994967, Length=351, Percent_Identity=28.7749287749288, Blast_Score=111, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003331 [H]

Pfam domain/function: PF02350 Epimerase_2 [H]

EC number: 5.1.3.14

Molecular weight: Translated: 47435; Mature: 47435

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVMTIVGTRPELIKLCCVISEFDKYTNHILVHTGQNYAYELNQVFFDDMGIRKPDYFL
CEEEEEEECCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHCCCCCCCCCEE
EVAADNTAKSIGLIIEQVDAVLEKEKPDAVLFYGDTNSCLSAIAAKRRKIPIFHMEAGNR
EEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEECCCCC
CFDQRVPEEINRKIIDHISDVNITLTEHARRYLISEGLPPELIFKSGSHMPEVLDRFMPK
HHHHCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHH
ILKSDILDTLSLQAKQYFLISSHREENVDVKNNLQELLSSLQTLIKEYNFPVIFSTHPRT
HHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
KKRLEDLGGGKELGDKIRFLPAFSFTDYVKLQMNAFCILSDSGTITEEASILNLPALNIR
HHHHHHCCCCHHHHHHEEEEECCCCCCEEEEEEEEEEEECCCCCCCCCCCEECCCCCCHH
EAHERPEGMDAGTLIMSGFNAARVLQSVKSITAEHENNKDTEEIYSNDLENCNVKRGTST
HHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCC
HSLYLIGGHASSPKFCRDNSSKQKSIVSDYAAAGLVSKKILRIVLSYVDYVNRTVWFKK
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCC
>Mature Secondary Structure
MLKVMTIVGTRPELIKLCCVISEFDKYTNHILVHTGQNYAYELNQVFFDDMGIRKPDYFL
CEEEEEEECCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHCCCCCCCCCEE
EVAADNTAKSIGLIIEQVDAVLEKEKPDAVLFYGDTNSCLSAIAAKRRKIPIFHMEAGNR
EEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEECCCCC
CFDQRVPEEINRKIIDHISDVNITLTEHARRYLISEGLPPELIFKSGSHMPEVLDRFMPK
HHHHCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHH
ILKSDILDTLSLQAKQYFLISSHREENVDVKNNLQELLSSLQTLIKEYNFPVIFSTHPRT
HHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
KKRLEDLGGGKELGDKIRFLPAFSFTDYVKLQMNAFCILSDSGTITEEASILNLPALNIR
HHHHHHCCCCHHHHHHEEEEECCCCCCEEEEEEEEEEEECCCCCCCCCCCEECCCCCCHH
EAHERPEGMDAGTLIMSGFNAARVLQSVKSITAEHENNKDTEEIYSNDLENCNVKRGTST
HHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCC
HSLYLIGGHASSPKFCRDNSSKQKSIVSDYAAAGLVSKKILRIVLSYVDYVNRTVWFKK
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: UDP-N-acetylglucosamine [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.63 {UDP-N-acetylglucosamine}} 1 {UDP-N-acetylmannosamine}} 0.5 {UDP-N-acetylmannosamine}} [C]

Substrates: UDP-N-acetyl-D-glucosamine

Specific reaction: UDP-N-acetyl-D-glucosamine = UDP-N-acetyl-D-mannosamine

General reaction: Epimerization [C]

Inhibitor: PCMB [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA