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The map label for this gene is gatA [H]

Identifier: 157825333

GI number: 157825333

Start: 207904

End: 209382

Strand: Reverse

Name: gatA [H]

Synonym: A1C_01075

Alternate gene names: 157825333

Gene position: 209382-207904 (Counterclockwise)

Preceding gene: 157825334

Following gene: 157825332

Centisome position: 17.01

GC content: 37.53

Gene sequence:

>1479_bases
ATGACAGAACTAAACAAATTAACAGTAGCAGACAGTATAAAAGGTTTAAAAAATAAAGATTTTACAAGTAAAGAATTAGT
TAATGCACATATTACACAAATAGAAAAACATAGAAATCTAAATGCTTATGTTACCGAAACTTTTGATTTAGCTTTAAAAC
AGGCTGAAGCGGCCGATCAAAATTATGCTCAAAATCAACCGCGAACACTTGAGGGTATCCCATTTGCTGCTAAAGATCTT
TTCTGTACAAAAGGCATTAGAACTACGGCATGTTCTAATATACTGAAAAATTTTGTACCTAATTATGAATCAAGCGTTAC
ACAAAATATTTTTGATAAGGGCGGCGTGATGCTCGGCAAAACAAATATGGATGAATTTGCGATGGGTTCAGCAAATATTA
CTAGTTGTTTCGGAAATGTAATTAGCCCTTGGAAAGCAAATGATGATAATGCTGATTTAGTTCCTGGCGGTTCTTCAGGT
GGTTCAGCTGCGGCAGTTAGTGGATTTATGGCCTCCGCGGCTCTTGGTAGCGATACTGGTGGCTCGGTACGTCAACCTGC
AAGCTTTACGGGTTTAGTTGGATTTAAACCGACATATGGTCGTTGCTCAAGATACGGGATGGTATCGTTTGCTAGCTCAC
TTGATCAAGCAGGCATATTCACTAGAAGCGTTTTAGACAGTAGCATTATGCTTGAAGCGATGATGGGGTTTGATGCAAAA
GATTCTACTTCGATTAAAGCAGAAGTGCCAGAATTACAATCCGCTATCGGAAGTTCTATGAAAAATATAAAGATAGGCGT
ACCTCTTAACCTTGGTGAAGGTGGTATTGAACCTGATGTTATGAAAATATGGCAGGATACCATAGAGCTACTTAAAAACG
CCGGTGCTGAAATAGTGGATATTATTTTGCCGCATGCTAAATACGGTGTTGCCGTTTATTACGTAATAGCACCGGCTGAA
GCTTCTTCAAATTTATCTAGATATGACGGCGTTAGATACGGTCTTCGAGTAGAACGTGAAAATATGACACTTGACGAAAT
GTATGAAATGACTAGATCAGCCGGATTTGGGGAGGAAGTAAAACGTCGTATTATGATTGGAACATATGTGCTTTCATCTA
ATTGTATGGATGCATATTATCTAAAAGCTCAAAAAGTACGTCGTTTAGTTGCAAACGATTTTAATAATGCTTTTGAAAAA
GTTGATGCCATTGTGTTACCTGCAGCACCGACTGAAGCATTTAAGATTGCTGAAAAACAAAATGATCCAACTATTATGTA
TTTAAACGATTTATTTACTATTCCCGCAAGTTTAGCCGGTTTACCTTGTGCATCAATTCCTGCTGGATTATCTGCACGAG
GTTTACCTCTTGGGATGCAGATTATAGGTAAACAATTAGATGAATATAATGTTTTGAAAGTAGCATCGACAATTGAGTCA
GGTGTAAAACATATTAAATTTGAGCCAGCAGGGTTTTAA

Upstream 100 bases:

>100_bases
CTGATTTATCAAACAAATTATTTGATAATGTAAGCGGAAATAGTGCGCAGCTTGCTAAAGAAGTAAAATATTTTATCACT
CCAAAGGTTGTTGAATAATT

Downstream 100 bases:

>100_bases
CCAAAGCTAGTAATTGATTTTATAAAGTTTTAGTAATTTAATGTATTGTACTGTATATTAATTTTTTACTTTGAAATCAA
GCGTAGGAGTGTTCAAAGCT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MTELNKLTVADSIKGLKNKDFTSKELVNAHITQIEKHRNLNAYVTETFDLALKQAEAADQNYAQNQPRTLEGIPFAAKDL
FCTKGIRTTACSNILKNFVPNYESSVTQNIFDKGGVMLGKTNMDEFAMGSANITSCFGNVISPWKANDDNADLVPGGSSG
GSAAAVSGFMASAALGSDTGGSVRQPASFTGLVGFKPTYGRCSRYGMVSFASSLDQAGIFTRSVLDSSIMLEAMMGFDAK
DSTSIKAEVPELQSAIGSSMKNIKIGVPLNLGEGGIEPDVMKIWQDTIELLKNAGAEIVDIILPHAKYGVAVYYVIAPAE
ASSNLSRYDGVRYGLRVERENMTLDEMYEMTRSAGFGEEVKRRIMIGTYVLSSNCMDAYYLKAQKVRRLVANDFNNAFEK
VDAIVLPAAPTEAFKIAEKQNDPTIMYLNDLFTIPASLAGLPCASIPAGLSARGLPLGMQIIGKQLDEYNVLKVASTIES
GVKHIKFEPAGF

Sequences:

>Translated_492_residues
MTELNKLTVADSIKGLKNKDFTSKELVNAHITQIEKHRNLNAYVTETFDLALKQAEAADQNYAQNQPRTLEGIPFAAKDL
FCTKGIRTTACSNILKNFVPNYESSVTQNIFDKGGVMLGKTNMDEFAMGSANITSCFGNVISPWKANDDNADLVPGGSSG
GSAAAVSGFMASAALGSDTGGSVRQPASFTGLVGFKPTYGRCSRYGMVSFASSLDQAGIFTRSVLDSSIMLEAMMGFDAK
DSTSIKAEVPELQSAIGSSMKNIKIGVPLNLGEGGIEPDVMKIWQDTIELLKNAGAEIVDIILPHAKYGVAVYYVIAPAE
ASSNLSRYDGVRYGLRVERENMTLDEMYEMTRSAGFGEEVKRRIMIGTYVLSSNCMDAYYLKAQKVRRLVANDFNNAFEK
VDAIVLPAAPTEAFKIAEKQNDPTIMYLNDLFTIPASLAGLPCASIPAGLSARGLPLGMQIIGKQLDEYNVLKVASTIES
GVKHIKFEPAGF
>Mature_491_residues
TELNKLTVADSIKGLKNKDFTSKELVNAHITQIEKHRNLNAYVTETFDLALKQAEAADQNYAQNQPRTLEGIPFAAKDLF
CTKGIRTTACSNILKNFVPNYESSVTQNIFDKGGVMLGKTNMDEFAMGSANITSCFGNVISPWKANDDNADLVPGGSSGG
SAAAVSGFMASAALGSDTGGSVRQPASFTGLVGFKPTYGRCSRYGMVSFASSLDQAGIFTRSVLDSSIMLEAMMGFDAKD
STSIKAEVPELQSAIGSSMKNIKIGVPLNLGEGGIEPDVMKIWQDTIELLKNAGAEIVDIILPHAKYGVAVYYVIAPAEA
SSNLSRYDGVRYGLRVERENMTLDEMYEMTRSAGFGEEVKRRIMIGTYVLSSNCMDAYYLKAQKVRRLVANDFNNAFEKV
DAIVLPAAPTEAFKIAEKQNDPTIMYLNDLFTIPASLAGLPCASIPAGLSARGLPLGMQIIGKQLDEYNVLKVASTIESG
VKHIKFEPAGF

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=490, Percent_Identity=40.8163265306122, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI195972892, Length=492, Percent_Identity=23.9837398373984, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17543272, Length=460, Percent_Identity=38.4782608695652, Blast_Score=304, Evalue=8e-83,
Organism=Caenorhabditis elegans, GI17556264, Length=290, Percent_Identity=25.5172413793103, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17537465, Length=248, Percent_Identity=27.4193548387097, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556278, Length=307, Percent_Identity=26.7100977198697, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=307, Percent_Identity=26.7100977198697, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71990152, Length=317, Percent_Identity=25.5520504731861, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=427, Percent_Identity=33.9578454332553, Blast_Score=247, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6319685, Length=414, Percent_Identity=27.2946859903382, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320448, Length=192, Percent_Identity=30.2083333333333, Blast_Score=63, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24648113, Length=466, Percent_Identity=39.4849785407725, Blast_Score=311, Evalue=6e-85,
Organism=Drosophila melanogaster, GI24644968, Length=506, Percent_Identity=25.296442687747, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24652985, Length=490, Percent_Identity=23.8775510204082, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19922090, Length=490, Percent_Identity=23.8775510204082, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24652981, Length=490, Percent_Identity=23.8775510204082, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24652983, Length=490, Percent_Identity=23.8775510204082, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI161078093, Length=521, Percent_Identity=24.1842610364683, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21356731, Length=485, Percent_Identity=23.9175257731959, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI45550774, Length=206, Percent_Identity=29.126213592233, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24648435, Length=206, Percent_Identity=29.126213592233, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648437, Length=206, Percent_Identity=29.126213592233, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53069; Mature: 52938

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELNKLTVADSIKGLKNKDFTSKELVNAHITQIEKHRNLNAYVTETFDLALKQAEAADQ
CCCCHHEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHH
NYAQNQPRTLEGIPFAAKDLFCTKGIRTTACSNILKNFVPNYESSVTQNIFDKGGVMLGK
HHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEC
TNMDEFAMGSANITSCFGNVISPWKANDDNADLVPGGSSGGSAAAVSGFMASAALGSDTG
CCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
GSVRQPASFTGLVGFKPTYGRCSRYGMVSFASSLDQAGIFTRSVLDSSIMLEAMMGFDAK
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DSTSIKAEVPELQSAIGSSMKNIKIGVPLNLGEGGIEPDVMKIWQDTIELLKNAGAEIVD
CCCCCEEHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEH
IILPHAKYGVAVYYVIAPAEASSNLSRYDGVRYGLRVERENMTLDEMYEMTRSAGFGEEV
HHHCCCCCCEEEEEEEECCCCCCCHHHHCCCEECEEEECCCCCHHHHHHHHHCCCCCHHH
KRRIMIGTYVLSSNCMDAYYLKAQKVRRLVANDFNNAFEKVDAIVLPAAPTEAFKIAEKQ
HHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHCC
NDPTIMYLNDLFTIPASLAGLPCASIPAGLSARGLPLGMQIIGKQLDEYNVLKVASTIES
CCCEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHH
GVKHIKFEPAGF
HHHEEECCCCCC
>Mature Secondary Structure 
TELNKLTVADSIKGLKNKDFTSKELVNAHITQIEKHRNLNAYVTETFDLALKQAEAADQ
CCCHHEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHH
NYAQNQPRTLEGIPFAAKDLFCTKGIRTTACSNILKNFVPNYESSVTQNIFDKGGVMLGK
HHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEC
TNMDEFAMGSANITSCFGNVISPWKANDDNADLVPGGSSGGSAAAVSGFMASAALGSDTG
CCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
GSVRQPASFTGLVGFKPTYGRCSRYGMVSFASSLDQAGIFTRSVLDSSIMLEAMMGFDAK
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DSTSIKAEVPELQSAIGSSMKNIKIGVPLNLGEGGIEPDVMKIWQDTIELLKNAGAEIVD
CCCCCEEHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEH
IILPHAKYGVAVYYVIAPAEASSNLSRYDGVRYGLRVERENMTLDEMYEMTRSAGFGEEV
HHHCCCCCCEEEEEEEECCCCCCCHHHHCCCEECEEEECCCCCHHHHHHHHHCCCCCHHH
KRRIMIGTYVLSSNCMDAYYLKAQKVRRLVANDFNNAFEKVDAIVLPAAPTEAFKIAEKQ
HHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHCC
NDPTIMYLNDLFTIPASLAGLPCASIPAGLSARGLPLGMQIIGKQLDEYNVLKVASTIES
CCCEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHH
GVKHIKFEPAGF
HHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA