Click here to switch to the map view.

The map label for this gene is gatB [H]

Identifier: 157825332

GI number: 157825332

Start: 206276

End: 207727

Strand: Reverse

Name: gatB [H]

Synonym: A1C_01070

Alternate gene names: 157825332

Gene position: 207727-206276 (Counterclockwise)

Preceding gene: 157825333

Following gene: 157825331

Centisome position: 16.87

GC content: 35.61

Gene sequence:

>1452_bases
ATGGCATATATTGAAGGTAATACGGGGAAATGGGAATATGTAATAGGACTTGAAATCCATGCTCAAATTTCCTCAAAATC
TAAGCTTTTTTCAGGAAGTAGTACTATTTTTGCAGCAAGTCCAAATTCGCAGGTTTCCTATGTTGATGCAGCAATGCCCG
GGATGTTACCGGTATTAAATCAGCACTGTGTACACCAAGCAATTAAAACAGGACTTGGGCTTAAAGCTAAAATAAATAAA
TATTCGGTATTTGATCGTAAAAATTATTTTTATGCCGATCTGCCGCAAGGTTACCAAATTTCACAATTTTACTATCCTAT
AGTACAAAACGGCACTATGGAAATACCGACTAGTACCGGCGATCTTAAAACTATTCGTATTAACCGTTTGCATTTAGAGC
AAGATGCTGGTAAATCTATGCATGATCAATCACCACATTACAGCTTTATCGATTTAAACCGTGCCGGTATTGGGCTTATG
GAAATTGTAACCGAACCTGATATATCATCACCGTATGAAGCGGCTGAATTTGTAAAAAAGCTGAGAAATTTGTTACGTTA
TATAGGCAGCTGCGACGGTGATATGGAAAAAGGCTCAATGCGTTGTGATGCTAATATTTCAGTACGACGTAGCGGCGAAC
CACTCGGCACAAGATGTGAAATTAAAAATATTAATTCAATTCGTAATATTGTTAAAGCAATAGAATTTGAAGCTAAAAGA
CAAGTAGATTTGCTTGAAAGCGGTGAAGCAATAATTCAAGAAACACGCTTATTTAATGCCGATAGCGGTGAAACAAGAAC
TATGCGTTTCAAAGAAGAAGCACTTGATTATAGATATTTTCCTGATCCTGATTTATTACCGCTTGTTATTTCCGATAAGT
TAATAGACGAGCTAAAAGCAAATTTACCTGAGCTACCTGATCAAAAGATTGAAAAATATACGAAGGAATTTGGCTTAAGT
AAATATGAGGCCGAAGTAATAGTAGCCGATGAATCAGTTGCTGACTATTTTGAGAAAGCAGTAAATGAATGTAACCCTAA
AATGCTTACTAATTGGCTAACTAGTGAGTTGTTCGGACAATTAAACAAAGCATCGATTGGAATAAATGAATGCAAAATTA
CTCCTAGTGACTTTGCAAAACTAATAAAATTAATAGAAAATGGTATTATTTCCGGTAAAATTGCAAAAACTGTTTTTGAA
ATTATGTTTGCAACCGGTAAAGCACCTGATAAAATAGTAGAGGAACAAGGACTTGTACAAGTTTCGGATAATAACGTGCT
AAAAACGGTCATTGACGAGGTGATAGCTGAAAATCCTGAATTGGTAGAGGGTTATAGAAGTGGTAAGGATAAATTATTCG
GTTTCTTTGTAGGGCAAGTAATGAAAAAAACTGGCGGTAAGGCTAATCCGACGCTTCTGAATCAGCTTTTAAATGAGAAA
TTAAGTTCTTGA

Upstream 100 bases:

>100_bases
TCAAGCGTAGGAGTGTTCAAAGCTTGGTTCCTAAAGTATTTTATTCAGTTAGTAATGACTTTAGTAATTGCTAACTGGGC
GTTCTTATGGGTTTTTAATT

Downstream 100 bases:

>100_bases
TGTTTGTGGTAATATCATGCTTTGTTGCACGGATACATATATCGGAACCGTCATTGCGAGGAGGCGTTGCCAACGCTGGT
CAATTTCGCCTATGTCATAC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MAYIEGNTGKWEYVIGLEIHAQISSKSKLFSGSSTIFAASPNSQVSYVDAAMPGMLPVLNQHCVHQAIKTGLGLKAKINK
YSVFDRKNYFYADLPQGYQISQFYYPIVQNGTMEIPTSTGDLKTIRINRLHLEQDAGKSMHDQSPHYSFIDLNRAGIGLM
EIVTEPDISSPYEAAEFVKKLRNLLRYIGSCDGDMEKGSMRCDANISVRRSGEPLGTRCEIKNINSIRNIVKAIEFEAKR
QVDLLESGEAIIQETRLFNADSGETRTMRFKEEALDYRYFPDPDLLPLVISDKLIDELKANLPELPDQKIEKYTKEFGLS
KYEAEVIVADESVADYFEKAVNECNPKMLTNWLTSELFGQLNKASIGINECKITPSDFAKLIKLIENGIISGKIAKTVFE
IMFATGKAPDKIVEEQGLVQVSDNNVLKTVIDEVIAENPELVEGYRSGKDKLFGFFVGQVMKKTGGKANPTLLNQLLNEK
LSS

Sequences:

>Translated_483_residues
MAYIEGNTGKWEYVIGLEIHAQISSKSKLFSGSSTIFAASPNSQVSYVDAAMPGMLPVLNQHCVHQAIKTGLGLKAKINK
YSVFDRKNYFYADLPQGYQISQFYYPIVQNGTMEIPTSTGDLKTIRINRLHLEQDAGKSMHDQSPHYSFIDLNRAGIGLM
EIVTEPDISSPYEAAEFVKKLRNLLRYIGSCDGDMEKGSMRCDANISVRRSGEPLGTRCEIKNINSIRNIVKAIEFEAKR
QVDLLESGEAIIQETRLFNADSGETRTMRFKEEALDYRYFPDPDLLPLVISDKLIDELKANLPELPDQKIEKYTKEFGLS
KYEAEVIVADESVADYFEKAVNECNPKMLTNWLTSELFGQLNKASIGINECKITPSDFAKLIKLIENGIISGKIAKTVFE
IMFATGKAPDKIVEEQGLVQVSDNNVLKTVIDEVIAENPELVEGYRSGKDKLFGFFVGQVMKKTGGKANPTLLNQLLNEK
LSS
>Mature_482_residues
AYIEGNTGKWEYVIGLEIHAQISSKSKLFSGSSTIFAASPNSQVSYVDAAMPGMLPVLNQHCVHQAIKTGLGLKAKINKY
SVFDRKNYFYADLPQGYQISQFYYPIVQNGTMEIPTSTGDLKTIRINRLHLEQDAGKSMHDQSPHYSFIDLNRAGIGLME
IVTEPDISSPYEAAEFVKKLRNLLRYIGSCDGDMEKGSMRCDANISVRRSGEPLGTRCEIKNINSIRNIVKAIEFEAKRQ
VDLLESGEAIIQETRLFNADSGETRTMRFKEEALDYRYFPDPDLLPLVISDKLIDELKANLPELPDQKIEKYTKEFGLSK
YEAEVIVADESVADYFEKAVNECNPKMLTNWLTSELFGQLNKASIGINECKITPSDFAKLIKLIENGIISGKIAKTVFEI
MFATGKAPDKIVEEQGLVQVSDNNVLKTVIDEVIAENPELVEGYRSGKDKLFGFFVGQVMKKTGGKANPTLLNQLLNEKL
SS

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=42.2222222222222, Blast_Score=394, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI115534094, Length=302, Percent_Identity=44.7019867549669, Blast_Score=232, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6319391, Length=512, Percent_Identity=33.0078125, Blast_Score=258, Evalue=1e-69,
Organism=Drosophila melanogaster, GI45550808, Length=490, Percent_Identity=38.5714285714286, Blast_Score=346, Evalue=2e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53995; Mature: 53864

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00867 CPSASE_2 ; PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYIEGNTGKWEYVIGLEIHAQISSKSKLFSGSSTIFAASPNSQVSYVDAAMPGMLPVLN
CEEEECCCCCEEEEEEEEEEEEECCCCCEECCCCEEEEECCCCCEEEEHHHCCCHHHHHH
QHCVHQAIKTGLGLKAKINKYSVFDRKNYFYADLPQGYQISQFYYPIVQNGTMEIPTSTG
HHHHHHHHHHCCCCEEECCCEEEEECCCCEEEECCCCCEEHHEEEHHHCCCEEECCCCCC
DLKTIRINRLHLEQDAGKSMHDQSPHYSFIDLNRAGIGLMEIVTEPDISSPYEAAEFVKK
CEEEEEEEEEEECHHCCCCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCHHHHHHHHH
LRNLLRYIGSCDGDMEKGSMRCDANISVRRSGEPLGTRCEIKNINSIRNIVKAIEFEAKR
HHHHHHHHHCCCCCCCCCCEEECCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHH
QVDLLESGEAIIQETRLFNADSGETRTMRFKEEALDYRYFPDPDLLPLVISDKLIDELKA
HHHHHHCCHHHHHHHHHCCCCCCCCEEEEEHHHHCCCCCCCCCCCEEHHHHHHHHHHHHH
NLPELPDQKIEKYTKEFGLSKYEAEVIVADESVADYFEKAVNECNPKMLTNWLTSELFGQ
CCCCCCHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
LNKASIGINECKITPSDFAKLIKLIENGIISGKIAKTVFEIMFATGKAPDKIVEEQGLVQ
CCCCCCCCCCEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCEE
VSDNNVLKTVIDEVIAENPELVEGYRSGKDKLFGFFVGQVMKKTGGKANPTLLNQLLNEK
ECCCCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LSS
HCC
>Mature Secondary Structure 
AYIEGNTGKWEYVIGLEIHAQISSKSKLFSGSSTIFAASPNSQVSYVDAAMPGMLPVLN
EEEECCCCCEEEEEEEEEEEEECCCCCEECCCCEEEEECCCCCEEEEHHHCCCHHHHHH
QHCVHQAIKTGLGLKAKINKYSVFDRKNYFYADLPQGYQISQFYYPIVQNGTMEIPTSTG
HHHHHHHHHHCCCCEEECCCEEEEECCCCEEEECCCCCEEHHEEEHHHCCCEEECCCCCC
DLKTIRINRLHLEQDAGKSMHDQSPHYSFIDLNRAGIGLMEIVTEPDISSPYEAAEFVKK
CEEEEEEEEEEECHHCCCCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCHHHHHHHHH
LRNLLRYIGSCDGDMEKGSMRCDANISVRRSGEPLGTRCEIKNINSIRNIVKAIEFEAKR
HHHHHHHHHCCCCCCCCCCEEECCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHH
QVDLLESGEAIIQETRLFNADSGETRTMRFKEEALDYRYFPDPDLLPLVISDKLIDELKA
HHHHHHCCHHHHHHHHHCCCCCCCCEEEEEHHHHCCCCCCCCCCCEEHHHHHHHHHHHHH
NLPELPDQKIEKYTKEFGLSKYEAEVIVADESVADYFEKAVNECNPKMLTNWLTSELFGQ
CCCCCCHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
LNKASIGINECKITPSDFAKLIKLIENGIISGKIAKTVFEIMFATGKAPDKIVEEQGLVQ
CCCCCCCCCCEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCEE
VSDNNVLKTVIDEVIAENPELVEGYRSGKDKLFGFFVGQVMKKTGGKANPTLLNQLLNEK
ECCCCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LSS
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA