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The map label for this gene is argG

Identifier: 157373011

GI number: 157373011

Start: 5294214

End: 5295434

Strand: Reverse

Name: argG

Synonym: Spro_4779

Alternate gene names: 157373011

Gene position: 5295434-5294214 (Counterclockwise)

Preceding gene: 157373012

Following gene: 157373010

Centisome position: 97.18

GC content: 56.67

Gene sequence:

>1221_bases
ATGCAAAACCAAGGCATCAAAAAAATCGTTCTGGCGTACTCTGGCGGTCTGGATACTTCGGCCATCATTCCCTGGCTGAA
AGAAAACTACGGCGGCTGCGAAGTGGTAGCCTTCGTGGCGGATATCGGCCAGGAGCGCAGCGATCTGGAAGGTGTGGAGC
AAAAAGCCCTGCAATCCGGTGCCTCTGAGTGCCACGTGGTTGATCTGCGGGAAGAATTCATCCGTGATTACGTTTACCCG
GTACTGCAGACCGGCGCCCTGTATGAAGGCAGCTACCTGCTGGGCACCTCCATGGCGCGCCCGATTATCGCTAAAGCGCA
GGTTGAACTGGCGCTGAAAGTGGGTGCTGACGCGGTGTGCCACGGCGCAACCGGTAAAGGTAATGACCAGGTGCGTTTCG
AAACCACCTATACCGCACTGGCCCCACATCTGAAAGTGGTTGCTCCTTGGCGTGAGTGGAACCTGCGTTCCCGTGAAGCG
CTGCTGGACTACCTGAAAGAGCGCAACATCCCGACCACCGCGTCGCTGGAAAAAATCTACAGCCGTGACGAGAATGCCTG
GCATATCTCTACCGAAGGCGGCGTGCTGGAAAGCCCGTGGAACGCACCGAACAAAGACTGCTGGGTCTGGACTGTCGATC
CGCAGGAAGCGCCGGATCAGCCTGAGCAAGTGACCATCACCGTAGAGAAAGGCTGCGTAGTGGCGGTTAACGGTGAAGCT
CTTAGCCCGTACAAATGTCTGGAAACCCTGAACGTGCTGGGTGCCAAGCATGGCGTGGGCCGTATCGATATCGTAGAAAA
CCGTCTGGTAGGCATCAAATCCCGCGGCTGCTACGAAACCCCGGGCGGCACCATTATGGTGGCGGCACTGCGTGGCGTTG
AACAGCTGGTGCTGGATCGCGACAGTTTCAAATGGCGTGAGCAACTGGGCCTGGAAATGTCTTACGTGGTATACGATGGC
CGTTGGTTTGCTCCACTGCGTCGTTCACTGCAGGCTTCCGCCGAAGCGCTGGCCGAAGAGGTTAACGGTGAAGTGGTGCT
GCAGCTGTATAAAGGCCAGGTGACGGCGATTCAGAAAAAATCCGCCAACAGCCTGTACTCCGAAGAGTTCGCGACCTTCG
GCGAAGACGAAGTTTACGATCACAGCCACGCGGGCGGTTTTATCCGTCTGTTCTCACTCTCTTCACGCATTCGCGCGCTG
AACGAGATCAAGAACAAGTAA

Upstream 100 bases:

>100_bases
GCCAGTTGGCGCCATGCCGATCAGCTTCCTGCACTGTTTAACGGCGTGTCAATTGGCACCCGGATCCTCGCTTAAATATA
GAATTAAAAAGGAAAAGACC

Downstream 100 bases:

>100_bases
CTTATTAATTATTCGGCGTGACTTGATAGGGGCGCAGCGTGCTGCGCCCCTATTCGCATAAAATCAGGAGTAAGGTATGG
CACTTTGGGGCGGACGGTTC

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MQNQGIKKIVLAYSGGLDTSAIIPWLKENYGGCEVVAFVADIGQERSDLEGVEQKALQSGASECHVVDLREEFIRDYVYP
VLQTGALYEGSYLLGTSMARPIIAKAQVELALKVGADAVCHGATGKGNDQVRFETTYTALAPHLKVVAPWREWNLRSREA
LLDYLKERNIPTTASLEKIYSRDENAWHISTEGGVLESPWNAPNKDCWVWTVDPQEAPDQPEQVTITVEKGCVVAVNGEA
LSPYKCLETLNVLGAKHGVGRIDIVENRLVGIKSRGCYETPGGTIMVAALRGVEQLVLDRDSFKWREQLGLEMSYVVYDG
RWFAPLRRSLQASAEALAEEVNGEVVLQLYKGQVTAIQKKSANSLYSEEFATFGEDEVYDHSHAGGFIRLFSLSSRIRAL
NEIKNK

Sequences:

>Translated_406_residues
MQNQGIKKIVLAYSGGLDTSAIIPWLKENYGGCEVVAFVADIGQERSDLEGVEQKALQSGASECHVVDLREEFIRDYVYP
VLQTGALYEGSYLLGTSMARPIIAKAQVELALKVGADAVCHGATGKGNDQVRFETTYTALAPHLKVVAPWREWNLRSREA
LLDYLKERNIPTTASLEKIYSRDENAWHISTEGGVLESPWNAPNKDCWVWTVDPQEAPDQPEQVTITVEKGCVVAVNGEA
LSPYKCLETLNVLGAKHGVGRIDIVENRLVGIKSRGCYETPGGTIMVAALRGVEQLVLDRDSFKWREQLGLEMSYVVYDG
RWFAPLRRSLQASAEALAEEVNGEVVLQLYKGQVTAIQKKSANSLYSEEFATFGEDEVYDHSHAGGFIRLFSLSSRIRAL
NEIKNK
>Mature_406_residues
MQNQGIKKIVLAYSGGLDTSAIIPWLKENYGGCEVVAFVADIGQERSDLEGVEQKALQSGASECHVVDLREEFIRDYVYP
VLQTGALYEGSYLLGTSMARPIIAKAQVELALKVGADAVCHGATGKGNDQVRFETTYTALAPHLKVVAPWREWNLRSREA
LLDYLKERNIPTTASLEKIYSRDENAWHISTEGGVLESPWNAPNKDCWVWTVDPQEAPDQPEQVTITVEKGCVVAVNGEA
LSPYKCLETLNVLGAKHGVGRIDIVENRLVGIKSRGCYETPGGTIMVAALRGVEQLVLDRDSFKWREQLGLEMSYVVYDG
RWFAPLRRSLQASAEALAEEVNGEVVLQLYKGQVTAIQKKSANSLYSEEFATFGEDEVYDHSHAGGFIRLFSLSSRIRAL
NEIKNK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=407, Percent_Identity=43.2432432432432, Blast_Score=308, Evalue=4e-84,
Organism=Homo sapiens, GI16950633, Length=407, Percent_Identity=43.2432432432432, Blast_Score=308, Evalue=4e-84,
Organism=Escherichia coli, GI1789563, Length=390, Percent_Identity=28.4615384615385, Blast_Score=139, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6324514, Length=413, Percent_Identity=40.6779661016949, Blast_Score=290, Evalue=2e-79,
Organism=Drosophila melanogaster, GI21358151, Length=407, Percent_Identity=40.7862407862408, Blast_Score=292, Evalue=3e-79,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_SERP5 (A8GL82)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001481000.1
- ProteinModelPortal:   A8GL82
- SMR:   A8GL82
- STRING:   A8GL82
- GeneID:   5604671
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4779
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- ProtClustDB:   PRK00509
- BioCyc:   SPRO399741:SPRO_4779-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44973; Mature: 44973

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 40-40 BINDING 92-92 BINDING 97-97 BINDING 122-122 BINDING 124-124 BINDING 128-128 BINDING 128-128 BINDING 129-129 BINDING 132-132 BINDING 181-181 BINDING 190-190 BINDING 266-266 BINDING 278-278

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNQGIKKIVLAYSGGLDTSAIIPWLKENYGGCEVVAFVADIGQERSDLEGVEQKALQSG
CCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
ASECHVVDLREEFIRDYVYPVLQTGALYEGSYLLGTSMARPIIAKAQVELALKVGADAVC
CCCEEEEHHHHHHHHHHHHHHHHCCCEECCCEEECCHHCCCHHHHHHEEEEEEECCCCEE
HGATGKGNDQVRFETTYTALAPHLKVVAPWREWNLRSREALLDYLKERNIPTTASLEKIY
ECCCCCCCCCEEEEEEHHHHCCCEEEEECHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHH
SRDENAWHISTEGGVLESPWNAPNKDCWVWTVDPQEAPDQPEQVTITVEKGCVVAVNGEA
CCCCCCEEEECCCCEECCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCEEEEECCCC
LSPYKCLETLNVLGAKHGVGRIDIVENRLVGIKSRGCYETPGGTIMVAALRGVEQLVLDR
CCHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCEECCCCEEEEHHHHHHHHHHHCC
DSFKWREQLGLEMSYVVYDGRWFAPLRRSLQASAEALAEEVNGEVVLQLYKGQVTAIQKK
CCCHHHHHHCCEEEEEEECCEECHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEHHH
SANSLYSEEFATFGEDEVYDHSHAGGFIRLFSLSSRIRALNEIKNK
CCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQNQGIKKIVLAYSGGLDTSAIIPWLKENYGGCEVVAFVADIGQERSDLEGVEQKALQSG
CCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
ASECHVVDLREEFIRDYVYPVLQTGALYEGSYLLGTSMARPIIAKAQVELALKVGADAVC
CCCEEEEHHHHHHHHHHHHHHHHCCCEECCCEEECCHHCCCHHHHHHEEEEEEECCCCEE
HGATGKGNDQVRFETTYTALAPHLKVVAPWREWNLRSREALLDYLKERNIPTTASLEKIY
ECCCCCCCCCEEEEEEHHHHCCCEEEEECHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHH
SRDENAWHISTEGGVLESPWNAPNKDCWVWTVDPQEAPDQPEQVTITVEKGCVVAVNGEA
CCCCCCEEEECCCCEECCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCEEEEECCCC
LSPYKCLETLNVLGAKHGVGRIDIVENRLVGIKSRGCYETPGGTIMVAALRGVEQLVLDR
CCHHHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCEECCCCEEEEHHHHHHHHHHHCC
DSFKWREQLGLEMSYVVYDGRWFAPLRRSLQASAEALAEEVNGEVVLQLYKGQVTAIQKK
CCCHHHHHHCCEEEEEEECCEECHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEHHH
SANSLYSEEFATFGEDEVYDHSHAGGFIRLFSLSSRIRALNEIKNK
CCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA