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The map label for this gene is iolD1 [H]

Identifier: 157372891

GI number: 157372891

Start: 5146601

End: 5148541

Strand: Reverse

Name: iolD1 [H]

Synonym: Spro_4659

Alternate gene names: 157372891

Gene position: 5148541-5146601 (Counterclockwise)

Preceding gene: 157372894

Following gene: 157372890

Centisome position: 94.49

GC content: 58.94

Gene sequence:

>1941_bases
ATGGGCAAGATCCGGTTAACCATGGCACAGGCACTCGTCAGGTTCCTTGATAACCAGTATCTGGTAGTCGATGGCGTGGA
AACCAAATTCGTCAAAGGCATTTTTGCGATTTTCGGTCACGGCAACGTACTCGGTCTGGGGCAAGCGCTGGAACAGGACA
GCGGTGATCTGATCGTGCATCAGGGGCGTAATGAGCAGGGCATGGCTCACGCAGCGACCGGTTTTGCCAAGCAGAAACTG
CGTCAACAGATCTACGCCTGCACCTCTTCTGTCGGTCCCGGCGCCGCCAATATGATCACCGCCGCTGCGACCGCGACCGC
CAACCGCATTCCTTTATTACTGTTGCCGGGCGATGTTTACGCTTCACGCCAACCGGACCCGGTGTTACAGCAGATTGAAC
AATCCTACGATCTCAGTATCAGCACCAACGACGCTTTCCGCGCGGTGAGCAAGTATTGGGATCGCATTGTGCGACCGGAA
CAGTTGATGAGCGCCTGTATCAACGCGATGCGCGTGCTGACCGATCCGGCGGAAACCGGTGCGGTGACGCTGAGTTTGCC
GCAGGACGTGCAGGGTGAGGCCTATGACTACCCTGACTACTTCTTCCAAAAACGCGTGCACCGGCTGGATCGCCGTCCGG
CGACCGAAGGCATGTTGGCCGACGCGCTGGCGCTGCTGACCACCAAGCGTCAACCGCTGCTGGTGTGCGGCGGTGGGGTG
AAATATTCGCAGGCCGGTCAGGCGCTGCGCGAGTTCGCCGAACGCTTCCGGATCCCGTTTGTGGAAACCCAGGCCGGCAA
GGGCACAGTCCCGTCCAACCACCCGTTTAACCTGGGCGGCATTGGTGAAACCGGCTGTCTGGCGGCCAATACCCTGGCCA
GACAGGCCGATCTGGTGATTGGCGTGGGCACCCGTTATACCGATTTCACTACTTCGTCCAAATGGCTGTTCCAGCATCCT
GACGTTGATTTTCTCAACGTCAACGTCAGCGCCTTCGACGCCGGTAAGCTCGACGGCCTGCAGGTACTGGCCGATGCCCG
TGAGGCACTGTCTGCCCTGGGGGCATTATTGGCTCAGGCCGATTACCGCGCCGGCTGGGGCAACGCCATTGCCGAAGCGC
GTAGCGCACAACAGCAGGAAACCGCCCGAGTTTATGCGGTGGAATACACCGGCGAAGGTTTTGTACCGGAAATTGACGAC
CACCTCGATCGCGACAGCGTGTTTGCCGAATTTATCGAAAAGACCGATTCACTGCTAACCCAAAGCCGGGTGCTGGGGGT
GCTAAACCAGCATCTGCCGCAGGATAGCGTGATCGTCGCCGCTGCCGGTAGCCTGCCGGGGGATTTACAGCGCGTGTGGC
AGAACCATGGCGAGCACGGCTACCACGTGGAATACGGTTATTCCTGCATGGGTTACGAGGTCAACGCCGCGCTGGGGGTC
AAGCTGGCAGAGCCGCAGCGTGAAGTGTACGCGATGGTCGGCGATGGCGCCTTTATGATGCTGCACTCCGAGCTGGTCAC
CTCAATTCAGGAGGGCTGCAAAATCAACGTGGTGCTGTTCGACAACATGACCAACGGCTGCATCAATAACCTGCAGATGG
AACACGGCATGGACAGCTACACCACCGAATTCCGCTTCCGCAACCCGCAGGGCGGCAAACTCGACGGCAAACTGGTTCCG
GTCAACTTCGCCATGCTGGCGGCGGCTTACGGTTGTAAAACCTACAGCGTGACCACCGAACAACAGCTGATCGAGGCGCT
GGCCGATGCTCGCCTGCAAAGCGTTTCTACCCTGCTGGACATCAAAGTGCTGCCAAAAACCATGGTGCACAAATATCTCA
GCTGGTGGCGCGTGGGCGGTGCTCAGGTGGCCGACAGCGAAAAAATTGTCGCCGTGGCCCGCAAGCTGCAAGAGAACATC
GACAAGGCACGCGACTACTGA

Upstream 100 bases:

>100_bases
AGCGCAGCGCGCTTGAAGGCAACGGGGACCACAGAGATTCCAGGCTCGGCATGCAGGTATTTTGCCGGAGCGGGCGCGAC
TAATCTAAAAGGGTGAGCAA

Downstream 100 bases:

>100_bases
TTAACACATTGCTTGATGACCCAGACCTTGCGTCTGCCGCAACTATTTGAAGAGGATAACGAAAATGACACTGAACATTG
GGGTTATTGGTACCGGCGCA

Product: thiamine pyrophosphate protein central region

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase 1 [H]

Number of amino acids: Translated: 646; Mature: 645

Protein sequence:

>646_residues
MGKIRLTMAQALVRFLDNQYLVVDGVETKFVKGIFAIFGHGNVLGLGQALEQDSGDLIVHQGRNEQGMAHAATGFAKQKL
RQQIYACTSSVGPGAANMITAAATATANRIPLLLLPGDVYASRQPDPVLQQIEQSYDLSISTNDAFRAVSKYWDRIVRPE
QLMSACINAMRVLTDPAETGAVTLSLPQDVQGEAYDYPDYFFQKRVHRLDRRPATEGMLADALALLTTKRQPLLVCGGGV
KYSQAGQALREFAERFRIPFVETQAGKGTVPSNHPFNLGGIGETGCLAANTLARQADLVIGVGTRYTDFTTSSKWLFQHP
DVDFLNVNVSAFDAGKLDGLQVLADAREALSALGALLAQADYRAGWGNAIAEARSAQQQETARVYAVEYTGEGFVPEIDD
HLDRDSVFAEFIEKTDSLLTQSRVLGVLNQHLPQDSVIVAAAGSLPGDLQRVWQNHGEHGYHVEYGYSCMGYEVNAALGV
KLAEPQREVYAMVGDGAFMMLHSELVTSIQEGCKINVVLFDNMTNGCINNLQMEHGMDSYTTEFRFRNPQGGKLDGKLVP
VNFAMLAAAYGCKTYSVTTEQQLIEALADARLQSVSTLLDIKVLPKTMVHKYLSWWRVGGAQVADSEKIVAVARKLQENI
DKARDY

Sequences:

>Translated_646_residues
MGKIRLTMAQALVRFLDNQYLVVDGVETKFVKGIFAIFGHGNVLGLGQALEQDSGDLIVHQGRNEQGMAHAATGFAKQKL
RQQIYACTSSVGPGAANMITAAATATANRIPLLLLPGDVYASRQPDPVLQQIEQSYDLSISTNDAFRAVSKYWDRIVRPE
QLMSACINAMRVLTDPAETGAVTLSLPQDVQGEAYDYPDYFFQKRVHRLDRRPATEGMLADALALLTTKRQPLLVCGGGV
KYSQAGQALREFAERFRIPFVETQAGKGTVPSNHPFNLGGIGETGCLAANTLARQADLVIGVGTRYTDFTTSSKWLFQHP
DVDFLNVNVSAFDAGKLDGLQVLADAREALSALGALLAQADYRAGWGNAIAEARSAQQQETARVYAVEYTGEGFVPEIDD
HLDRDSVFAEFIEKTDSLLTQSRVLGVLNQHLPQDSVIVAAAGSLPGDLQRVWQNHGEHGYHVEYGYSCMGYEVNAALGV
KLAEPQREVYAMVGDGAFMMLHSELVTSIQEGCKINVVLFDNMTNGCINNLQMEHGMDSYTTEFRFRNPQGGKLDGKLVP
VNFAMLAAAYGCKTYSVTTEQQLIEALADARLQSVSTLLDIKVLPKTMVHKYLSWWRVGGAQVADSEKIVAVARKLQENI
DKARDY
>Mature_645_residues
GKIRLTMAQALVRFLDNQYLVVDGVETKFVKGIFAIFGHGNVLGLGQALEQDSGDLIVHQGRNEQGMAHAATGFAKQKLR
QQIYACTSSVGPGAANMITAAATATANRIPLLLLPGDVYASRQPDPVLQQIEQSYDLSISTNDAFRAVSKYWDRIVRPEQ
LMSACINAMRVLTDPAETGAVTLSLPQDVQGEAYDYPDYFFQKRVHRLDRRPATEGMLADALALLTTKRQPLLVCGGGVK
YSQAGQALREFAERFRIPFVETQAGKGTVPSNHPFNLGGIGETGCLAANTLARQADLVIGVGTRYTDFTTSSKWLFQHPD
VDFLNVNVSAFDAGKLDGLQVLADAREALSALGALLAQADYRAGWGNAIAEARSAQQQETARVYAVEYTGEGFVPEIDDH
LDRDSVFAEFIEKTDSLLTQSRVLGVLNQHLPQDSVIVAAAGSLPGDLQRVWQNHGEHGYHVEYGYSCMGYEVNAALGVK
LAEPQREVYAMVGDGAFMMLHSELVTSIQEGCKINVVLFDNMTNGCINNLQMEHGMDSYTTEFRFRNPQGGKLDGKLVPV
NFAMLAAAYGCKTYSVTTEQQLIEALADARLQSVSTLLDIKVLPKTMVHKYLSWWRVGGAQVADSEKIVAVARKLQENID
KARDY

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=559, Percent_Identity=21.4669051878354, Blast_Score=100, Evalue=7e-21,
Organism=Escherichia coli, GI1790104, Length=582, Percent_Identity=25.9450171821306, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI87081685, Length=576, Percent_Identity=26.7361111111111, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1786717, Length=544, Percent_Identity=25.3676470588235, Blast_Score=126, Evalue=5e-30,
Organism=Escherichia coli, GI1787096, Length=528, Percent_Identity=22.1590909090909, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788716, Length=294, Percent_Identity=25.8503401360544, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323755, Length=571, Percent_Identity=24.6935201401051, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922626, Length=608, Percent_Identity=23.0263157894737, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 70661; Mature: 70530

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKIRLTMAQALVRFLDNQYLVVDGVETKFVKGIFAIFGHGNVLGLGQALEQDSGDLIVH
CCCEEHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCEEECCHHHCCCCCCEEEE
QGRNEQGMAHAATGFAKQKLRQQIYACTSSVGPGAANMITAAATATANRIPLLLLPGDVY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
ASRQPDPVLQQIEQSYDLSISTNDAFRAVSKYWDRIVRPEQLMSACINAMRVLTDPAETG
CCCCCCHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AVTLSLPQDVQGEAYDYPDYFFQKRVHRLDRRPATEGMLADALALLTTKRQPLLVCGGGV
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCC
KYSQAGQALREFAERFRIPFVETQAGKGTVPSNHPFNLGGIGETGCLAANTLARQADLVI
CHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEE
GVGTRYTDFTTSSKWLFQHPDVDFLNVNVSAFDAGKLDGLQVLADAREALSALGALLAQA
ECCCCEECCCCCCCEEEECCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHC
DYRAGWGNAIAEARSAQQQETARVYAVEYTGEGFVPEIDDHLDRDSVFAEFIEKTDSLLT
CCCCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHHHHH
QSRVLGVLNQHLPQDSVIVAAAGSLPGDLQRVWQNHGEHGYHVEYGYSCMGYEVNAALGV
HHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEECCEEECEEECCEECE
KLAEPQREVYAMVGDGAFMMLHSELVTSIQEGCKINVVLFDNMTNGCINNLQMEHGMDSY
EECCCHHHEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHCCCHHCCCCCC
TTEFRFRNPQGGKLDGKLVPVNFAMLAAAYGCKTYSVTTEQQLIEALADARLQSVSTLLD
EEEEEEECCCCCCCCCEEEEHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
IKVLPKTMVHKYLSWWRVGGAQVADSEKIVAVARKLQENIDKARDY
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GKIRLTMAQALVRFLDNQYLVVDGVETKFVKGIFAIFGHGNVLGLGQALEQDSGDLIVH
CCEEHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCEEECCHHHCCCCCCEEEE
QGRNEQGMAHAATGFAKQKLRQQIYACTSSVGPGAANMITAAATATANRIPLLLLPGDVY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
ASRQPDPVLQQIEQSYDLSISTNDAFRAVSKYWDRIVRPEQLMSACINAMRVLTDPAETG
CCCCCCHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AVTLSLPQDVQGEAYDYPDYFFQKRVHRLDRRPATEGMLADALALLTTKRQPLLVCGGGV
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCC
KYSQAGQALREFAERFRIPFVETQAGKGTVPSNHPFNLGGIGETGCLAANTLARQADLVI
CHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEE
GVGTRYTDFTTSSKWLFQHPDVDFLNVNVSAFDAGKLDGLQVLADAREALSALGALLAQA
ECCCCEECCCCCCCEEEECCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHC
DYRAGWGNAIAEARSAQQQETARVYAVEYTGEGFVPEIDDHLDRDSVFAEFIEKTDSLLT
CCCCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHHHHH
QSRVLGVLNQHLPQDSVIVAAAGSLPGDLQRVWQNHGEHGYHVEYGYSCMGYEVNAALGV
HHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEECCEEECEEECCEECE
KLAEPQREVYAMVGDGAFMMLHSELVTSIQEGCKINVVLFDNMTNGCINNLQMEHGMDSY
EECCCHHHEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHCCCHHCCCCCC
TTEFRFRNPQGGKLDGKLVPVNFAMLAAAYGCKTYSVTTEQQLIEALADARLQSVSTLLD
EEEEEEECCCCCCCCCEEEEHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
IKVLPKTMVHKYLSWWRVGGAQVADSEKIVAVARKLQENIDKARDY
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA