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The map label for this gene is glgB

Identifier: 157372879

GI number: 157372879

Start: 5132732

End: 5134918

Strand: Reverse

Name: glgB

Synonym: Spro_4647

Alternate gene names: 157372879

Gene position: 5134918-5132732 (Counterclockwise)

Preceding gene: 157372880

Following gene: 157372878

Centisome position: 94.24

GC content: 58.85

Gene sequence:

>2187_bases
ATGCCTGTACTTCCCGATCGTGACGTGATTGATCAGCTTTTCTCCGGCAATTCTGCCGACCCCTTTTCTCTGCTGGGTAT
GCATGCGGCCGATAACGGTCTGCAGGTGCGTGCGCTGTTGCCTGATGCCAGCGAAGTCTGGCTGCTGGAGCAGCAAACCG
GCCGGCGTCTGGTGCAGCTAAACTGCGACGATGCGCGCGGTTTTTTCAGCGCCACGGTACCGCGTCGCAAAACCCCGTTC
CGCTATCAATTCGAAGTTCGCTGGCAGGATCATCAGCAGATCGTCGACGATCCCTACCGTTTCGGCACCCTGCTGCAGGA
TATTGACAGTTGGTTGCTGGCCGAAGGCACCCATTTGCGTCCTTATGAACGTCTGGGGGCACACCTCAGTACGCTTGATG
ACGTCGAAGGCGTCAGCTTTGCCGTTTGGGCGCCGAATGCCCAGCGCGTCTCGGTGGTTGGCGAGTTTAACTTCTGGGAC
GGCCGCCGCCACCCGATGCGCCTGCGGCGTGAAAACGGGATTTGGGAGCTGTTCCTGCCGGGGGTGAAAGCCGGGCAATT
GTACAAGTACGAAATTATAGACTGCTACGGCAATACCCAGTTGAAGGCCGATCCTTACGCCTTCGAGGCGCAAATGCGGC
CGGATACCGCGTCGCTGGTGACGCCGTTGCCGGAGGTGGTGGAAAACACCCCGCAACGCCAGCGGGCCAATGATTTTGAC
CGACCGATTTCGATCTACGAGGTGCATCTTGGCTCGTGGCGGCGTCACACCGACGATAATTTCTGGCTCAGCTATGGCGA
ACTGGCCGTGCAGCTGATTGATTACGTCAAGGACATGGGGTTCACCCATATCGAGCTGCTGCCGATTAACGAGCATCCGT
TTGACGGCAGTTGGGGCTATCAGCCGTTGGGGTTGTACGCCCCGACCCGGCGTTTCGGTACCCCGGCCGACTTCAAAGAT
TTTGTCGCGGCAGCGCACCGGGCGGGCATCAACGTGATCCTGGACTGGGTGCCGGGGCATTTCCCCAGCGATGCCTATGG
CCTGGCGAATTTCGACGGCACCGCGCTGTATGAATACGCCGACCCGCGCGAAGGCTTCCATCAGGACTGGAATACCCTGA
TTTACAACTATGGCCGCCATGAGGTGCGTAACTATCTGGCGGGTAATGCCCTTTACTGGTTGGAACGCTATGGCATCGAC
GCATTGCGTGTCGATGCGGTGGCTTCGATGATCTACCGCGATTACAGCCGTGCCGAAGGCGAATGGGTACCGAACTACTA
CGGTGGCAACGAAAATCTGGAGGCCATTGCCTTCCTGCGTTACACCAACCAGAGCGTCGGCAAGGAGCGGCCCGGTGCGG
TGACGCTGGCGGAAGAGTCCACCGATTACCCAGGGGTAACGCTGCCGCCGGAATCTAACGGGCTGGGGTTCCACTACAAA
TGGAATCTGGGTTGGATGCACGACACGCTCAACTACATGCAATGCGACCCGGTACACCGCAAGTATCATCACAATCAGAT
GACCTTCGGCATGTTGTACGCCTACACCGAAAACTTCGTGCTGCCGATTTCCCATGACGAAGTGGTGCACGGCAAGAAAT
CGATTCTCGACCGCATGCCCGGCGACGCCTGGCAGAAGTTTGCCAACCTGCGTGCCTACTACGGCTTTATGTGGGCGCAC
CCGGGCAAAAAACTGCTGTTTATGGGCTGCGAGTTCGCCCAGGGGCGTGAATGGAACTTCGACAGCAGCCTCGACTGGCA
TCTGCTGGAAGGGCTGGACGGCTGGCATCACGGGGTGCAGCGGTTGGTGCGCGATCTTAACCATTGCTATCAACAGCAGG
CGCCGTTGTATGAGCGTGACTACCGGCCGGACGGCTTTGAGTGGCTGGTGGTGGACGACCATGACAATTCGGTGTTTGCC
TTCGCCCGCTATGATGCGCATGGCAATGAGCTGATCGCTGTCAGCAACTTTACCCCGGTGCCACGTCACCACTATCGCAT
CGGAATTTCCCGCCCCGGTGAGTACCGGGAAATCCTCAATACCGACTCGCACCATTATCACGGCAGCAATACCGGCAACC
AGGGGCGCGTGGTGTCCGAGCAGATAGGCAACCACGGCCGCGAGCATTCGATTAGCGTGACCGTGCCGCCGCTGGCGACC
ATCTACCTGCTGCGGGAGGCGCAATGA

Upstream 100 bases:

>100_bases
CCCGGTGGATCTCAAGGTGCAGCCATTTCGCTGCAACCTGAAAGACGATGAGCTTACGCGGCCCCTTATAAATCATGCTC
TTAATCTTAGGAAGAAGACT

Downstream 100 bases:

>100_bases
CTGAGCTGACGCCCGGCATCGCCGCGCCTCTCGGGGCGTACTATGACGGTAACGGCATCAACTTTAGCCTGTATTCCGCC
CATGCTACCGGGGTGGAGCT

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 728; Mature: 727

Protein sequence:

>728_residues
MPVLPDRDVIDQLFSGNSADPFSLLGMHAADNGLQVRALLPDASEVWLLEQQTGRRLVQLNCDDARGFFSATVPRRKTPF
RYQFEVRWQDHQQIVDDPYRFGTLLQDIDSWLLAEGTHLRPYERLGAHLSTLDDVEGVSFAVWAPNAQRVSVVGEFNFWD
GRRHPMRLRRENGIWELFLPGVKAGQLYKYEIIDCYGNTQLKADPYAFEAQMRPDTASLVTPLPEVVENTPQRQRANDFD
RPISIYEVHLGSWRRHTDDNFWLSYGELAVQLIDYVKDMGFTHIELLPINEHPFDGSWGYQPLGLYAPTRRFGTPADFKD
FVAAAHRAGINVILDWVPGHFPSDAYGLANFDGTALYEYADPREGFHQDWNTLIYNYGRHEVRNYLAGNALYWLERYGID
ALRVDAVASMIYRDYSRAEGEWVPNYYGGNENLEAIAFLRYTNQSVGKERPGAVTLAEESTDYPGVTLPPESNGLGFHYK
WNLGWMHDTLNYMQCDPVHRKYHHNQMTFGMLYAYTENFVLPISHDEVVHGKKSILDRMPGDAWQKFANLRAYYGFMWAH
PGKKLLFMGCEFAQGREWNFDSSLDWHLLEGLDGWHHGVQRLVRDLNHCYQQQAPLYERDYRPDGFEWLVVDDHDNSVFA
FARYDAHGNELIAVSNFTPVPRHHYRIGISRPGEYREILNTDSHHYHGSNTGNQGRVVSEQIGNHGREHSISVTVPPLAT
IYLLREAQ

Sequences:

>Translated_728_residues
MPVLPDRDVIDQLFSGNSADPFSLLGMHAADNGLQVRALLPDASEVWLLEQQTGRRLVQLNCDDARGFFSATVPRRKTPF
RYQFEVRWQDHQQIVDDPYRFGTLLQDIDSWLLAEGTHLRPYERLGAHLSTLDDVEGVSFAVWAPNAQRVSVVGEFNFWD
GRRHPMRLRRENGIWELFLPGVKAGQLYKYEIIDCYGNTQLKADPYAFEAQMRPDTASLVTPLPEVVENTPQRQRANDFD
RPISIYEVHLGSWRRHTDDNFWLSYGELAVQLIDYVKDMGFTHIELLPINEHPFDGSWGYQPLGLYAPTRRFGTPADFKD
FVAAAHRAGINVILDWVPGHFPSDAYGLANFDGTALYEYADPREGFHQDWNTLIYNYGRHEVRNYLAGNALYWLERYGID
ALRVDAVASMIYRDYSRAEGEWVPNYYGGNENLEAIAFLRYTNQSVGKERPGAVTLAEESTDYPGVTLPPESNGLGFHYK
WNLGWMHDTLNYMQCDPVHRKYHHNQMTFGMLYAYTENFVLPISHDEVVHGKKSILDRMPGDAWQKFANLRAYYGFMWAH
PGKKLLFMGCEFAQGREWNFDSSLDWHLLEGLDGWHHGVQRLVRDLNHCYQQQAPLYERDYRPDGFEWLVVDDHDNSVFA
FARYDAHGNELIAVSNFTPVPRHHYRIGISRPGEYREILNTDSHHYHGSNTGNQGRVVSEQIGNHGREHSISVTVPPLAT
IYLLREAQ
>Mature_727_residues
PVLPDRDVIDQLFSGNSADPFSLLGMHAADNGLQVRALLPDASEVWLLEQQTGRRLVQLNCDDARGFFSATVPRRKTPFR
YQFEVRWQDHQQIVDDPYRFGTLLQDIDSWLLAEGTHLRPYERLGAHLSTLDDVEGVSFAVWAPNAQRVSVVGEFNFWDG
RRHPMRLRRENGIWELFLPGVKAGQLYKYEIIDCYGNTQLKADPYAFEAQMRPDTASLVTPLPEVVENTPQRQRANDFDR
PISIYEVHLGSWRRHTDDNFWLSYGELAVQLIDYVKDMGFTHIELLPINEHPFDGSWGYQPLGLYAPTRRFGTPADFKDF
VAAAHRAGINVILDWVPGHFPSDAYGLANFDGTALYEYADPREGFHQDWNTLIYNYGRHEVRNYLAGNALYWLERYGIDA
LRVDAVASMIYRDYSRAEGEWVPNYYGGNENLEAIAFLRYTNQSVGKERPGAVTLAEESTDYPGVTLPPESNGLGFHYKW
NLGWMHDTLNYMQCDPVHRKYHHNQMTFGMLYAYTENFVLPISHDEVVHGKKSILDRMPGDAWQKFANLRAYYGFMWAHP
GKKLLFMGCEFAQGREWNFDSSLDWHLLEGLDGWHHGVQRLVRDLNHCYQQQAPLYERDYRPDGFEWLVVDDHDNSVFAF
ARYDAHGNELIAVSNFTPVPRHHYRIGISRPGEYREILNTDSHHYHGSNTGNQGRVVSEQIGNHGREHSISVTVPPLATI
YLLREAQ

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=709, Percent_Identity=25.5289139633286, Blast_Score=169, Evalue=9e-42,
Organism=Escherichia coli, GI1789839, Length=723, Percent_Identity=72.1991701244813, Blast_Score=1116, Evalue=0.0,
Organism=Escherichia coli, GI2367229, Length=344, Percent_Identity=25.2906976744186, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17554896, Length=660, Percent_Identity=25.9090909090909, Blast_Score=175, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI32564391, Length=612, Percent_Identity=25.8169934640523, Blast_Score=152, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6320826, Length=683, Percent_Identity=25.0366032210835, Blast_Score=151, Evalue=3e-37,
Organism=Drosophila melanogaster, GI28573410, Length=638, Percent_Identity=27.4294670846395, Blast_Score=160, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_SERP5 (A8GKV0)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480868.1
- ProteinModelPortal:   A8GKV0
- SMR:   A8GKV0
- STRING:   A8GKV0
- GeneID:   5603141
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4647
- eggNOG:   COG0296
- HOGENOM:   HBG287139
- OMA:   RMQASAI
- ProtClustDB:   PRK05402
- BioCyc:   SPRO399741:SPRO_4647-MONOMER
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 83975; Mature: 83843

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: ACT_SITE 300-300 ACT_SITE 335-335 ACT_SITE 340-340 ACT_SITE 403-403 ACT_SITE 405-405 ACT_SITE 458-458 ACT_SITE 525-525 ACT_SITE 526-526

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLPDRDVIDQLFSGNSADPFSLLGMHAADNGLQVRALLPDASEVWLLEQQTGRRLVQL
CCCCCCHHHHHHHHCCCCCCCHHEEEEEECCCCEEEEEECCCCCCEEEEECCCCCEEEEE
NCDDARGFFSATVPRRKTPFRYQFEVRWQDHQQIVDDPYRFGTLLQDIDSWLLAEGTHLR
ECCCCCCCEEECCCCCCCCEEEEEEEEECCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCC
PYERLGAHLSTLDDVEGVSFAVWAPNAQRVSVVGEFNFWDGRRHPMRLRRENGIWELFLP
HHHHHCCHHHHHHCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCHHHHHCCCEEEEEEC
GVKAGQLYKYEIIDCYGNTQLKADPYAFEAQMRPDTASLVTPLPEVVENTPQRQRANDFD
CCCCCCEEEEEEEEECCCCEECCCCEEEEEECCCCHHHHHCCCHHHHCCCCCHHHCCCCC
RPISIYEVHLGSWRRHTDDNFWLSYGELAVQLIDYVKDMGFTHIELLPINEHPFDGSWGY
CCEEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
QPLGLYAPTRRFGTPADFKDFVAAAHRAGINVILDWVPGHFPSDAYGLANFDGTALYEYA
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEEECC
DPREGFHQDWNTLIYNYGRHEVRNYLAGNALYWLERYGIDALRVDAVASMIYRDYSRAEG
CCCHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEHHHCCCCHHHHHHHHHHHHHHHHHCCC
EWVPNYYGGNENLEAIAFLRYTNQSVGKERPGAVTLAEESTDYPGVTLPPESNGLGFHYK
CCCCCCCCCCCCCEEEEEEEECCHHCCCCCCCEEEEECCCCCCCCEECCCCCCCCEEEEE
WNLGWMHDTLNYMQCDPVHRKYHHNQMTFGMLYAYTENFVLPISHDEVVHGKKSILDRMP
EECCCHHHCCCHHCCCHHHHHHCCCCCEEEEEEEECCCEEEECCCCHHHCCHHHHHHCCC
GDAWQKFANLRAYYGFMWAHPGKKLLFMGCEFAQGREWNFDSSLDWHLLEGLDGWHHGVQ
CHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCCHHHHHHH
RLVRDLNHCYQQQAPLYERDYRPDGFEWLVVDDHDNSVFAFARYDAHGNELIAVSNFTPV
HHHHHHHHHHHHCCCCHHCCCCCCCEEEEEEECCCCCEEEEEEECCCCCEEEEECCCCCC
PRHHYRIGISRPGEYREILNTDSHHYHGSNTGNQGRVVSEQIGNHGREHSISVTVPPLAT
CCCEEEEECCCCCHHHHHHCCCCCEECCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCEE
IYLLREAQ
EEEEECCC
>Mature Secondary Structure 
PVLPDRDVIDQLFSGNSADPFSLLGMHAADNGLQVRALLPDASEVWLLEQQTGRRLVQL
CCCCCHHHHHHHHCCCCCCCHHEEEEEECCCCEEEEEECCCCCCEEEEECCCCCEEEEE
NCDDARGFFSATVPRRKTPFRYQFEVRWQDHQQIVDDPYRFGTLLQDIDSWLLAEGTHLR
ECCCCCCCEEECCCCCCCCEEEEEEEEECCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCC
PYERLGAHLSTLDDVEGVSFAVWAPNAQRVSVVGEFNFWDGRRHPMRLRRENGIWELFLP
HHHHHCCHHHHHHCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCHHHHHCCCEEEEEEC
GVKAGQLYKYEIIDCYGNTQLKADPYAFEAQMRPDTASLVTPLPEVVENTPQRQRANDFD
CCCCCCEEEEEEEEECCCCEECCCCEEEEEECCCCHHHHHCCCHHHHCCCCCHHHCCCCC
RPISIYEVHLGSWRRHTDDNFWLSYGELAVQLIDYVKDMGFTHIELLPINEHPFDGSWGY
CCEEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
QPLGLYAPTRRFGTPADFKDFVAAAHRAGINVILDWVPGHFPSDAYGLANFDGTALYEYA
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEEECC
DPREGFHQDWNTLIYNYGRHEVRNYLAGNALYWLERYGIDALRVDAVASMIYRDYSRAEG
CCCHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEHHHCCCCHHHHHHHHHHHHHHHHHCCC
EWVPNYYGGNENLEAIAFLRYTNQSVGKERPGAVTLAEESTDYPGVTLPPESNGLGFHYK
CCCCCCCCCCCCCEEEEEEEECCHHCCCCCCCEEEEECCCCCCCCEECCCCCCCCEEEEE
WNLGWMHDTLNYMQCDPVHRKYHHNQMTFGMLYAYTENFVLPISHDEVVHGKKSILDRMP
EECCCHHHCCCHHCCCHHHHHHCCCCCEEEEEEEECCCEEEECCCCHHHCCHHHHHHCCC
GDAWQKFANLRAYYGFMWAHPGKKLLFMGCEFAQGREWNFDSSLDWHLLEGLDGWHHGVQ
CHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCCHHHHHHH
RLVRDLNHCYQQQAPLYERDYRPDGFEWLVVDDHDNSVFAFARYDAHGNELIAVSNFTPV
HHHHHHHHHHHHCCCCHHCCCCCCCEEEEEEECCCCCEEEEEEECCCCCEEEEECCCCCC
PRHHYRIGISRPGEYREILNTDSHHYHGSNTGNQGRVVSEQIGNHGREHSISVTVPPLAT
CCCEEEEECCCCCHHHHHHCCCCCEECCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCEE
IYLLREAQ
EEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA