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The map label for this gene is glgX

Identifier: 157372878

GI number: 157372878

Start: 5130750

End: 5132735

Strand: Reverse

Name: glgX

Synonym: Spro_4646

Alternate gene names: 157372878

Gene position: 5132735-5130750 (Counterclockwise)

Preceding gene: 157372879

Following gene: 157372877

Centisome position: 94.2

GC content: 61.93

Gene sequence:

>1986_bases
ATGACTGAGCTGACGCCCGGCATCGCCGCGCCTCTCGGGGCGTACTATGACGGTAACGGCATCAACTTTAGCCTGTATTC
CGCCCATGCTACCGGGGTGGAGCTGTGCCTGTTTGACGAACAACAGCGTGAAGTTCGCCTGCCGTTGGCGACGCGCAGCG
GTGATATTTGGCACGGTTATCTGCCGGGCGGCAAACCGGGCCAGCGTTATGGCTACCGGGTGCATGGCCCGTTTGATCCT
GCGCAGGGGCTGCGTTTTAACCCCAATAAGCTGCTGCTGGATCCCGCCGCCCGCGCGGTCGAGGGGCCGGTGGCCGATGA
CCCCTACCTGCATGGTGGCTATGACCGGCCGGATCGGCATGACAGCGCCGAGCTGATGCCAAAGTGCGTGGTGATCGACG
AAGCCTACGACTGGCAGGATGATTGCCCGCCGGCCACGCCGTGGGGCAAAACGGTGATCTACGAAGCGCATGTGCGCGGT
CTGACCCTGCAACATCCGGAGATCCCGCAGGTGCTGCGTGGCAGTTTTGCCGCGCTCGGCCACCCGGTGATGATCGCCTA
CTTCAAGCGCCTTGGCATTACCGCGCTGGAACTGCTGCCGGTGCAGCAACACAGCTCCGAGCCGCGCCTGCAGCGCCTCG
GGTTGATTAACTACTGGGGCTATAACGTGCTGGCGCCCTATGCGCCGGATAACCGTTACTCCAGCCTGCGTACCGACATG
ACGCCGCTGCGCGAATTCCGCGACGCGGTGAAGGCGCTGCATAAGGCCGGGATTGAAGTGATCCTCGACGTGGTATTCAA
CCACAGCGCCGAGCTGGATACCGACGGGCCGACGCTGTCGCTGCGTGGCATCGATAACCCGGGCTATTACTGGCTCACTC
CCGACGGTGGCTACGTGAACGATACCGGCTGCGGTAACACGCTGCGGCTTGATCAACCACAGGGCGTAGCCTGGGTGATG
GACTGCCTGCGTTTTTGGGTGGGGGAGTGTCACGTAGACGGCTTCCGCTTTGATCTGGGCAGCGTGCTCGGCCGCACGCC
GGCGTTCGATCGCGACGCGCCGCTGTTCCAGGCGATGCTGGCCGACGACCTGCTGTCACGCTGCAAGTTGATTGCCGAAC
CCTGGGATATTGGTCCCGGCGGCTATCAGGTGGGTGAGTTTCCCGGGCGTTTTGCCGAGTGGAATGACCATTACCGGGAC
GATATGCGCCGCTTCTGGCTGCAGGGCGATATTTCGCTCGGTCAGTTCGCCCAGCGTTTTGCCGCTTCCAGTGACCTGTT
CAACCAACGCGGGCGTGCGCCGTACGCCAGCATCAACATGCTGACCGCTCATGACGGTTTTACCCTGCAGGATCTGGTCA
GCTTTAGCCGTAAGCACAACCAACTGAACGGCGAAGGTAACCGCGACGGCAGCGACCGTAACTTTAGCAACAACCACGGT
GTTGAAGGCCCGATAGCCGACGACGCCATTGTGCAACGCCGTAGGGCCAGCCGGCAGGCACTGCTTGCCACGTTATTGCT
GTCCCAGGGAACGCCGATGCTGCTGGCCGGCGATGAACATGGACACAGCCAGCAGGGCAACAACAATGCCTATTGTCAGG
ACAACGCTATCACCTGGCTCGACTGGGCCACGGCGGATGACTCACTCACCGCTTATACCGCCGCACTGATCCACCTGCGC
CAACAAATTCCCGCCCTGCAGCATGACCGCTGGTGGCAGGAGGGCGACGGTAACGTGCAGTGGCTGAACGCGCAGGGGCA
ATCGCTCAGCGCGCAACAGTGGGAGCAGGGAGATCTGCAGTTGCAGATCTGTCTGTCACAACGCTGGCTGGTGGTAGTGA
ACGCCACGCAGCAGGCGGTGGAGATGACGTTGCCTGCCGGCGATTGGCAGCTTGTCGCCCCCTTTACTCAGGAAGATTCG
CGGGCGGTCTTACCCGCCTGGAACCAGGCTGCGCACTCGATTTGCGTGCTGGTCAAGAAAAAATAA

Upstream 100 bases:

>100_bases
GGCGCGTGGTGTCCGAGCAGATAGGCAACCACGGCCGCGAGCATTCGATTAGCGTGACCGTGCCGCCGCTGGCGACCATC
TACCTGCTGCGGGAGGCGCA

Downstream 100 bases:

>100_bases
AAGGAGTCAGCTATGGTTAGGTTTGAAAATAAAGACCCCCTGATGTTGGCACGCCAACTGCCGATTAAGTCCGTCGCCCT
GATTTTGGCCGGTGGCCGCG

Product: glycogen debranching protein

Products: NA

Alternate protein names: Glycogen operon protein GlgX

Number of amino acids: Translated: 661; Mature: 660

Protein sequence:

>661_residues
MTELTPGIAAPLGAYYDGNGINFSLYSAHATGVELCLFDEQQREVRLPLATRSGDIWHGYLPGGKPGQRYGYRVHGPFDP
AQGLRFNPNKLLLDPAARAVEGPVADDPYLHGGYDRPDRHDSAELMPKCVVIDEAYDWQDDCPPATPWGKTVIYEAHVRG
LTLQHPEIPQVLRGSFAALGHPVMIAYFKRLGITALELLPVQQHSSEPRLQRLGLINYWGYNVLAPYAPDNRYSSLRTDM
TPLREFRDAVKALHKAGIEVILDVVFNHSAELDTDGPTLSLRGIDNPGYYWLTPDGGYVNDTGCGNTLRLDQPQGVAWVM
DCLRFWVGECHVDGFRFDLGSVLGRTPAFDRDAPLFQAMLADDLLSRCKLIAEPWDIGPGGYQVGEFPGRFAEWNDHYRD
DMRRFWLQGDISLGQFAQRFAASSDLFNQRGRAPYASINMLTAHDGFTLQDLVSFSRKHNQLNGEGNRDGSDRNFSNNHG
VEGPIADDAIVQRRRASRQALLATLLLSQGTPMLLAGDEHGHSQQGNNNAYCQDNAITWLDWATADDSLTAYTAALIHLR
QQIPALQHDRWWQEGDGNVQWLNAQGQSLSAQQWEQGDLQLQICLSQRWLVVVNATQQAVEMTLPAGDWQLVAPFTQEDS
RAVLPAWNQAAHSICVLVKKK

Sequences:

>Translated_661_residues
MTELTPGIAAPLGAYYDGNGINFSLYSAHATGVELCLFDEQQREVRLPLATRSGDIWHGYLPGGKPGQRYGYRVHGPFDP
AQGLRFNPNKLLLDPAARAVEGPVADDPYLHGGYDRPDRHDSAELMPKCVVIDEAYDWQDDCPPATPWGKTVIYEAHVRG
LTLQHPEIPQVLRGSFAALGHPVMIAYFKRLGITALELLPVQQHSSEPRLQRLGLINYWGYNVLAPYAPDNRYSSLRTDM
TPLREFRDAVKALHKAGIEVILDVVFNHSAELDTDGPTLSLRGIDNPGYYWLTPDGGYVNDTGCGNTLRLDQPQGVAWVM
DCLRFWVGECHVDGFRFDLGSVLGRTPAFDRDAPLFQAMLADDLLSRCKLIAEPWDIGPGGYQVGEFPGRFAEWNDHYRD
DMRRFWLQGDISLGQFAQRFAASSDLFNQRGRAPYASINMLTAHDGFTLQDLVSFSRKHNQLNGEGNRDGSDRNFSNNHG
VEGPIADDAIVQRRRASRQALLATLLLSQGTPMLLAGDEHGHSQQGNNNAYCQDNAITWLDWATADDSLTAYTAALIHLR
QQIPALQHDRWWQEGDGNVQWLNAQGQSLSAQQWEQGDLQLQICLSQRWLVVVNATQQAVEMTLPAGDWQLVAPFTQEDS
RAVLPAWNQAAHSICVLVKKK
>Mature_660_residues
TELTPGIAAPLGAYYDGNGINFSLYSAHATGVELCLFDEQQREVRLPLATRSGDIWHGYLPGGKPGQRYGYRVHGPFDPA
QGLRFNPNKLLLDPAARAVEGPVADDPYLHGGYDRPDRHDSAELMPKCVVIDEAYDWQDDCPPATPWGKTVIYEAHVRGL
TLQHPEIPQVLRGSFAALGHPVMIAYFKRLGITALELLPVQQHSSEPRLQRLGLINYWGYNVLAPYAPDNRYSSLRTDMT
PLREFRDAVKALHKAGIEVILDVVFNHSAELDTDGPTLSLRGIDNPGYYWLTPDGGYVNDTGCGNTLRLDQPQGVAWVMD
CLRFWVGECHVDGFRFDLGSVLGRTPAFDRDAPLFQAMLADDLLSRCKLIAEPWDIGPGGYQVGEFPGRFAEWNDHYRDD
MRRFWLQGDISLGQFAQRFAASSDLFNQRGRAPYASINMLTAHDGFTLQDLVSFSRKHNQLNGEGNRDGSDRNFSNNHGV
EGPIADDAIVQRRRASRQALLATLLLSQGTPMLLAGDEHGHSQQGNNNAYCQDNAITWLDWATADDSLTAYTAALIHLRQ
QIPALQHDRWWQEGDGNVQWLNAQGQSLSAQQWEQGDLQLQICLSQRWLVVVNATQQAVEMTLPAGDWQLVAPFTQEDSR
AVLPAWNQAAHSICVLVKKK

Specific function: Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Escherichia coli, GI2367229, Length=659, Percent_Identity=62.8224582701062, Blast_Score=864, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGX_SERP5 (A8GKU9)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480867.1
- ProteinModelPortal:   A8GKU9
- SMR:   A8GKU9
- STRING:   A8GKU9
- GeneID:   5603140
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4646
- eggNOG:   COG1523
- HOGENOM:   HBG350946
- OMA:   EMALDEF
- ProtClustDB:   PRK03705
- BioCyc:   SPRO399741:SPRO_4646-MONOMER
- HAMAP:   MF_01248
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR022844
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR02100

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: 3.2.1.- [C]

Molecular weight: Translated: 73764; Mature: 73633

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: ACT_SITE 338-338 ACT_SITE 373-373

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELTPGIAAPLGAYYDGNGINFSLYSAHATGVELCLFDEQQREVRLPLATRSGDIWHGY
CCCCCCCHHCCCCCEECCCCCEEEEEECCCCCEEEEEECCCCCEEECEEEECCCCEEEEE
LPGGKPGQRYGYRVHGPFDPAQGLRFNPNKLLLDPAARAVEGPVADDPYLHGGYDRPDRH
CCCCCCCCCCCEEEECCCCCCCCCEECCCEEEECCHHHHHCCCCCCCCCCCCCCCCCCCC
DSAELMPKCVVIDEAYDWQDDCPPATPWGKTVIYEAHVRGLTLQHPEIPQVLRGSFAALG
CCHHHCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCHHHHHHHHHHHC
HPVMIAYFKRLGITALELLPVQQHSSEPRLQRLGLINYWGYNVLAPYAPDNRYSSLRTDM
CHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHCCEEEECCCCEECCCCCCCCHHHHHCCH
TPLREFRDAVKALHKAGIEVILDVVFNHSAELDTDGPTLSLRGIDNPGYYWLTPDGGYVN
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEECCCCCEEC
DTGCGNTLRLDQPQGVAWVMDCLRFWVGECHVDGFRFDLGSVLGRTPAFDRDAPLFQAML
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCEEECHHHHHCCCCCCCCCCHHHHHHH
ADDLLSRCKLIAEPWDIGPGGYQVGEFPGRFAEWNDHYRDDMRRFWLQGDISLGQFAQRF
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
AASSDLFNQRGRAPYASINMLTAHDGFTLQDLVSFSRKHNQLNGEGNRDGSDRNFSNNHG
HHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
VEGPIADDAIVQRRRASRQALLATLLLSQGTPMLLAGDEHGHSQQGNNNAYCQDNAITWL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEECCCCEEEE
DWATADDSLTAYTAALIHLRQQIPALQHDRWWQEGDGNVQWLNAQGQSLSAQQWEQGDLQ
EECCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCEEEEECCCCCCCHHHHCCCCEE
LQICLSQRWLVVVNATQQAVEMTLPAGDWQLVAPFTQEDSRAVLPAWNQAAHSICVLVKK
EEEEECCCEEEEEECCCEEEEEECCCCCCEEEECCCCCCCCEECCCHHHCCCEEEEEEEC
K
C
>Mature Secondary Structure 
TELTPGIAAPLGAYYDGNGINFSLYSAHATGVELCLFDEQQREVRLPLATRSGDIWHGY
CCCCCCHHCCCCCEECCCCCEEEEEECCCCCEEEEEECCCCCEEECEEEECCCCEEEEE
LPGGKPGQRYGYRVHGPFDPAQGLRFNPNKLLLDPAARAVEGPVADDPYLHGGYDRPDRH
CCCCCCCCCCCEEEECCCCCCCCCEECCCEEEECCHHHHHCCCCCCCCCCCCCCCCCCCC
DSAELMPKCVVIDEAYDWQDDCPPATPWGKTVIYEAHVRGLTLQHPEIPQVLRGSFAALG
CCHHHCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCHHHHHHHHHHHC
HPVMIAYFKRLGITALELLPVQQHSSEPRLQRLGLINYWGYNVLAPYAPDNRYSSLRTDM
CHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHCCEEEECCCCEECCCCCCCCHHHHHCCH
TPLREFRDAVKALHKAGIEVILDVVFNHSAELDTDGPTLSLRGIDNPGYYWLTPDGGYVN
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEECCCCCEEC
DTGCGNTLRLDQPQGVAWVMDCLRFWVGECHVDGFRFDLGSVLGRTPAFDRDAPLFQAML
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCEEECHHHHHCCCCCCCCCCHHHHHHH
ADDLLSRCKLIAEPWDIGPGGYQVGEFPGRFAEWNDHYRDDMRRFWLQGDISLGQFAQRF
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
AASSDLFNQRGRAPYASINMLTAHDGFTLQDLVSFSRKHNQLNGEGNRDGSDRNFSNNHG
HHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
VEGPIADDAIVQRRRASRQALLATLLLSQGTPMLLAGDEHGHSQQGNNNAYCQDNAITWL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEECCCCEEEE
DWATADDSLTAYTAALIHLRQQIPALQHDRWWQEGDGNVQWLNAQGQSLSAQQWEQGDLQ
EECCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCEEEEECCCCCCCHHHHCCCCEE
LQICLSQRWLVVVNATQQAVEMTLPAGDWQLVAPFTQEDSRAVLPAWNQAAHSICVLVKK
EEEEECCCEEEEEECCCEEEEEECCCCCCEEEECCCCCCCCEECCCHHHCCCEEEEEEEC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA