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The map label for this gene is purR [H]

Identifier: 157372805

GI number: 157372805

Start: 5047368

End: 5048348

Strand: Direct

Name: purR [H]

Synonym: Spro_4573

Alternate gene names: 157372805

Gene position: 5047368-5048348 (Clockwise)

Preceding gene: 157372794

Following gene: 157372808

Centisome position: 92.63

GC content: 59.84

Gene sequence:

>981_bases
ATGTCGATCGAAAAGGTGGCGAAACTCGCCGGCGTCTCTGCCGCGACGGTCTCACGGGTATTGAACGGCCAGCAAATCGT
CAAACCGGCCACTCGCGACAAGGTACTGGCGGCGATCCGCGCATGCGACTACCAACCCAACCTGCTGGCGCGTCAGCTAC
GCACTTCGCGTAGCCGTATGCTGCTGGTACTGGTTTCCAGTATCACCAATCCTTTTTGTTCCCTGGTAGTACGCGGTATC
GAAGAAGAAGCCGAACGCCACGGCTACCACATTTTGCTGTGCAACTCCGAATCCAATCCAACCCGGGAGTCCGCCTATCT
CAGCCTGCTGAGCGGTAAAGTGGTCGACGGCGTGATTACCATGGACGCCGTCAGTTGCCTGCCCGGCCTGACCGCAATGA
TTGGCGATTTTCCCTGGGTACAATGCGGCGAAGGCGATCCCGAGTTCCGTGCCTCTTCAGTCACCATCGACAACCATCAG
GCGGCGAGGGCCGGCGTGCGTTATTTAGCTGCACGGGGCCGACGACGTATTGCCCTGATCAACAGCGACATGCGCTATCT
CTACTCGCAGCAGCGAGAGCAAGGTTATCGACAGGCGCTGGCCGAAGCCGGATTCGACTATTGTCAGGTGGAATACGTCG
AGGGGTTGGAATATGCCGCCGGTGCGTTGGCCATGGCTCGCCTGCTGGATGCCGGGCCGCGACCGGATGCGGTATTCACC
ATCTCCGACGTGATCGCCGGTGGGGCGATGAGCACCCTGCACCAACAGGGATTGCGCATTCCCGAAGATGTCGCGGTAAT
GGGCTTTGACGGCGTGCCATTCGGGGCCGTCACCACGCCACCGCTGACCACCGTCGAACAACCGATGCACCAGTTCGGCG
TGCGCAGCGTGCAGTTGCTGCTGGAAAAAATCAAAAATCCGCAACAACCACCGCGCAATGAAGTGCTTGAGTGGCGGCTG
GTTGAGCGTGGCTCAGCCTGA

Upstream 100 bases:

>100_bases
TGAATTTGTAACCCACATCACAATCCTGAGGCCAGGCGCAGACTTTTTGCCGCGCTTGGGCATATTGTGCGGGGCAATTG
GGCAGAAGGAACAGCAAACC

Downstream 100 bases:

>100_bases
TTACAAGCTCAATTCCCCACGCAAAAACGTGTGTGCATAGCCGCCAATCAAGGTGCGATCGCCCTTCAGCTCACAGAACA
ACTCGCCGCCGCGCGCCGAA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSIEKVAKLAGVSAATVSRVLNGQQIVKPATRDKVLAAIRACDYQPNLLARQLRTSRSRMLLVLVSSITNPFCSLVVRGI
EEEAERHGYHILLCNSESNPTRESAYLSLLSGKVVDGVITMDAVSCLPGLTAMIGDFPWVQCGEGDPEFRASSVTIDNHQ
AARAGVRYLAARGRRRIALINSDMRYLYSQQREQGYRQALAEAGFDYCQVEYVEGLEYAAGALAMARLLDAGPRPDAVFT
ISDVIAGGAMSTLHQQGLRIPEDVAVMGFDGVPFGAVTTPPLTTVEQPMHQFGVRSVQLLLEKIKNPQQPPRNEVLEWRL
VERGSA

Sequences:

>Translated_326_residues
MSIEKVAKLAGVSAATVSRVLNGQQIVKPATRDKVLAAIRACDYQPNLLARQLRTSRSRMLLVLVSSITNPFCSLVVRGI
EEEAERHGYHILLCNSESNPTRESAYLSLLSGKVVDGVITMDAVSCLPGLTAMIGDFPWVQCGEGDPEFRASSVTIDNHQ
AARAGVRYLAARGRRRIALINSDMRYLYSQQREQGYRQALAEAGFDYCQVEYVEGLEYAAGALAMARLLDAGPRPDAVFT
ISDVIAGGAMSTLHQQGLRIPEDVAVMGFDGVPFGAVTTPPLTTVEQPMHQFGVRSVQLLLEKIKNPQQPPRNEVLEWRL
VERGSA
>Mature_325_residues
SIEKVAKLAGVSAATVSRVLNGQQIVKPATRDKVLAAIRACDYQPNLLARQLRTSRSRMLLVLVSSITNPFCSLVVRGIE
EEAERHGYHILLCNSESNPTRESAYLSLLSGKVVDGVITMDAVSCLPGLTAMIGDFPWVQCGEGDPEFRASSVTIDNHQA
ARAGVRYLAARGRRRIALINSDMRYLYSQQREQGYRQALAEAGFDYCQVEYVEGLEYAAGALAMARLLDAGPRPDAVFTI
SDVIAGGAMSTLHQQGLRIPEDVAVMGFDGVPFGAVTTPPLTTVEQPMHQFGVRSVQLLLEKIKNPQQPPRNEVLEWRLV
ERGSA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=330, Percent_Identity=35.7575757575758, Blast_Score=184, Evalue=5e-48,
Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=34.9544072948328, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI1787948, Length=329, Percent_Identity=32.5227963525836, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1788474, Length=329, Percent_Identity=29.483282674772, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI48994940, Length=326, Percent_Identity=30.3680981595092, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1789202, Length=332, Percent_Identity=30.421686746988, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1787580, Length=322, Percent_Identity=30.1242236024845, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1789068, Length=293, Percent_Identity=30.0341296928328, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1790715, Length=316, Percent_Identity=30.0632911392405, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786540, Length=340, Percent_Identity=27.9411764705882, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1786268, Length=335, Percent_Identity=25.3731343283582, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787906, Length=333, Percent_Identity=26.1261261261261, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789456, Length=339, Percent_Identity=25.0737463126844, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790689, Length=322, Percent_Identity=22.6708074534161, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35628; Mature: 35497

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEKVAKLAGVSAATVSRVLNGQQIVKPATRDKVLAAIRACDYQPNLLARQLRTSRSRM
CCHHHHHHHHCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LLVLVSSITNPFCSLVVRGIEEEAERHGYHILLCNSESNPTRESAYLSLLSGKVVDGVIT
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCHHHHHHHH
MDAVSCLPGLTAMIGDFPWVQCGEGDPEFRASSVTIDNHQAARAGVRYLAARGRRRIALI
HHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEE
NSDMRYLYSQQREQGYRQALAEAGFDYCQVEYVEGLEYAAGALAMARLLDAGPRPDAVFT
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
ISDVIAGGAMSTLHQQGLRIPEDVAVMGFDGVPFGAVTTPPLTTVEQPMHQFGVRSVQLL
HHHHHCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LEKIKNPQQPPRNEVLEWRLVERGSA
HHHHCCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SIEKVAKLAGVSAATVSRVLNGQQIVKPATRDKVLAAIRACDYQPNLLARQLRTSRSRM
CHHHHHHHHCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LLVLVSSITNPFCSLVVRGIEEEAERHGYHILLCNSESNPTRESAYLSLLSGKVVDGVIT
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCHHHHHHHH
MDAVSCLPGLTAMIGDFPWVQCGEGDPEFRASSVTIDNHQAARAGVRYLAARGRRRIALI
HHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEE
NSDMRYLYSQQREQGYRQALAEAGFDYCQVEYVEGLEYAAGALAMARLLDAGPRPDAVFT
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
ISDVIAGGAMSTLHQQGLRIPEDVAVMGFDGVPFGAVTTPPLTTVEQPMHQFGVRSVQLL
HHHHHCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LEKIKNPQQPPRNEVLEWRLVERGSA
HHHHCCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA