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The map label for this gene is kefB [H]

Identifier: 157372792

GI number: 157372792

Start: 5033698

End: 5035506

Strand: Reverse

Name: kefB [H]

Synonym: Spro_4560

Alternate gene names: 157372792

Gene position: 5035506-5033698 (Counterclockwise)

Preceding gene: 157372793

Following gene: 157372791

Centisome position: 92.41

GC content: 59.7

Gene sequence:

>1809_bases
ATGGAAGGCTCAACCTTACTGACCGCAATTTTACTGTTTCTGTTCGCTGCGGTGGTCACGGTGCCGATTGCACGCCGCCT
GGGTATTGGCGCGGTCTTGGGCTACCTGATCGCCGGCATTGCCATCGGCCCCTGGGGGCTGGGCTTCATTCGCGACGTCG
ACGAGATCCTGCACTTTTCCGAACTGGGCGTGGTGTTTCTGATGTTTATCATCGGCCTGGAGCTGAACCCCAGCAAGCTG
TGGGAGTTGCGGCGTTCAATCTTTGGCGTCGGTGCCGGACAGGTGCTGATCACCGCCGCGGTGCTGGGGGCCTTGCTTTA
TCTGACCCATTTTGCCTGGCAGGCCGCCGTGATAGGCGGTATCGGGCTGGCGATGTCGTCAACCGCCATGGCGTTGCAGC
TAATGCGCGAGAAGGGCATGAACCGCAATGAGGGCGGCCAACTGGGGTTCTCGGTGCTGCTGTTCCAGGATATGGCGGTG
ATCCCGGCGCTGGCGCTGATCCCGATCCTCGCCGGGGCCGGGGGCAGCAGTGACGACTGGGCACAAATTGCACTCAAGGT
GGCGGCGTTCGGCGGCATGCTGATTGGCGGGCGGTTCCTGTTGCGGCCACTGTTCCGCTATATCGCCTCTTCCGGAGTAC
GCGAAATCTTCACCGCTGCCGCTCTGCTGGTGGTGTTGGGGGCGGCGATGTTTATGGATGCGCTCGGCCTGTCGATGGCG
CTCGGCACCTTTATCGCCGGCGTGTTGCTGGCGGAGAGTGAATATCGCCATGAGCTGGAAATTTCAATCGAGCCATTCAA
GGGGCTGCTGCTGGGGCTGTTCTTTATCTCGGTGGGCATGGCGCTGAATATCGGCGTACTCTATACCCATCTGGCCGAAG
TGCTGCTGGGCGTGGTGCTGCTGGTGGCGATCAAGTCGGGCGTCCTGTATTCGCTGTCGCGTATTTTTGGCCTGCGCAGT
TCGGTGCGTTTGCAGTTTGCCGGCGTGCTTAGCCAGGGCGGCGAGTTTGCCTTCGTACTGTTTTCGGCGGCGGGCACGCA
GAAGGTGCTGCAGCCCGATCAACTCGCGCTGTTGCTGGTGGTGGTGACCTTGTCGATGATGACTACGCCGCTGTTGATGC
AAATTATTGACCGTATTCTGGTGCGCCGATACAACGCCAGGGATGAAGACGAAGAGACACCCTATGTGGAGGACGATGAC
CCGCAGGTGATCATCGTCGGCTTCGGGCGTTTTGGTCAGGTGATCGGCCGATTGCTGATGGCCAACAAGATGCGCATCAC
CGTATTGGAGCGCGATGTCAGCGCCGTTGGCGTGCTGCGCCGCTACGGTTACAAGGTATATTACGGCGACGCCACCGAGC
TGGAGCTGCTGCGTGCGGCCGGTGCGGCGAAGGCCAGGTCGATCGTGATCACCTGTAATGAGCCGGAAGACACTATGGAA
ATTGTGCGGCTGTGTCAGCAGCATTTCCCCGATCTGAGCATTTTGGCGCGAGCACGCGGTCGTGTTGAAGCGCACGAATT
GCTGCAGGCCGGGGTCAAGCAGTTCAGCCGCGAAACCTTCTCCAGCGCGCTGGAACTGGGGCGAAAGGCACTGATGGAGT
TAGGTATGCATCCGCATCAGGCCTATCGTGCGCAACAGCACTTCCGCCGGCTGGACATGCGTATGCTGCGTGAATTGATG
CCGCCGCATCACGGCGATGTGGCGCAAATTTCCCGCGTGAAAGAAGCGCGGCGTGAGTTGGAAGAACTTTTCCACCGAGA
AATGCAAAAAGAGAGTCGCCAGTTCGACGGCTGGGATGAATACGAATAA

Upstream 100 bases:

>100_bases
TGTATTGGGCGCGTCGGCAGAAGCCAGAGGTGCTGGAAAGCCATGCCACGGCGTATGGCGACTGGTTGCGCAACCCGCTG
CCACACGGAGGGAGGTAAAC

Downstream 100 bases:

>100_bases
TTTGGAGCACTGAGATGTCTGCAACACGTAAAAGATTTATCGCCGGAGCAGTCTGCCCGAGCTGTAAGGCTATGGATACG
CTGGCAGTTTGGCGTGAAGA

Product: glutathione-regulated potassium-efflux system protein KefB

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 602; Mature: 602

Protein sequence:

>602_residues
MEGSTLLTAILLFLFAAVVTVPIARRLGIGAVLGYLIAGIAIGPWGLGFIRDVDEILHFSELGVVFLMFIIGLELNPSKL
WELRRSIFGVGAGQVLITAAVLGALLYLTHFAWQAAVIGGIGLAMSSTAMALQLMREKGMNRNEGGQLGFSVLLFQDMAV
IPALALIPILAGAGGSSDDWAQIALKVAAFGGMLIGGRFLLRPLFRYIASSGVREIFTAAALLVVLGAAMFMDALGLSMA
LGTFIAGVLLAESEYRHELEISIEPFKGLLLGLFFISVGMALNIGVLYTHLAEVLLGVVLLVAIKSGVLYSLSRIFGLRS
SVRLQFAGVLSQGGEFAFVLFSAAGTQKVLQPDQLALLLVVVTLSMMTTPLLMQIIDRILVRRYNARDEDEETPYVEDDD
PQVIIVGFGRFGQVIGRLLMANKMRITVLERDVSAVGVLRRYGYKVYYGDATELELLRAAGAAKARSIVITCNEPEDTME
IVRLCQQHFPDLSILARARGRVEAHELLQAGVKQFSRETFSSALELGRKALMELGMHPHQAYRAQQHFRRLDMRMLRELM
PPHHGDVAQISRVKEARRELEELFHREMQKESRQFDGWDEYE

Sequences:

>Translated_602_residues
MEGSTLLTAILLFLFAAVVTVPIARRLGIGAVLGYLIAGIAIGPWGLGFIRDVDEILHFSELGVVFLMFIIGLELNPSKL
WELRRSIFGVGAGQVLITAAVLGALLYLTHFAWQAAVIGGIGLAMSSTAMALQLMREKGMNRNEGGQLGFSVLLFQDMAV
IPALALIPILAGAGGSSDDWAQIALKVAAFGGMLIGGRFLLRPLFRYIASSGVREIFTAAALLVVLGAAMFMDALGLSMA
LGTFIAGVLLAESEYRHELEISIEPFKGLLLGLFFISVGMALNIGVLYTHLAEVLLGVVLLVAIKSGVLYSLSRIFGLRS
SVRLQFAGVLSQGGEFAFVLFSAAGTQKVLQPDQLALLLVVVTLSMMTTPLLMQIIDRILVRRYNARDEDEETPYVEDDD
PQVIIVGFGRFGQVIGRLLMANKMRITVLERDVSAVGVLRRYGYKVYYGDATELELLRAAGAAKARSIVITCNEPEDTME
IVRLCQQHFPDLSILARARGRVEAHELLQAGVKQFSRETFSSALELGRKALMELGMHPHQAYRAQQHFRRLDMRMLRELM
PPHHGDVAQISRVKEARRELEELFHREMQKESRQFDGWDEYE
>Mature_602_residues
MEGSTLLTAILLFLFAAVVTVPIARRLGIGAVLGYLIAGIAIGPWGLGFIRDVDEILHFSELGVVFLMFIIGLELNPSKL
WELRRSIFGVGAGQVLITAAVLGALLYLTHFAWQAAVIGGIGLAMSSTAMALQLMREKGMNRNEGGQLGFSVLLFQDMAV
IPALALIPILAGAGGSSDDWAQIALKVAAFGGMLIGGRFLLRPLFRYIASSGVREIFTAAALLVVLGAAMFMDALGLSMA
LGTFIAGVLLAESEYRHELEISIEPFKGLLLGLFFISVGMALNIGVLYTHLAEVLLGVVLLVAIKSGVLYSLSRIFGLRS
SVRLQFAGVLSQGGEFAFVLFSAAGTQKVLQPDQLALLLVVVTLSMMTTPLLMQIIDRILVRRYNARDEDEETPYVEDDD
PQVIIVGFGRFGQVIGRLLMANKMRITVLERDVSAVGVLRRYGYKVYYGDATELELLRAAGAAKARSIVITCNEPEDTME
IVRLCQQHFPDLSILARARGRVEAHELLQAGVKQFSRETFSSALELGRKALMELGMHPHQAYRAQQHFRRLDMRMLRELM
PPHHGDVAQISRVKEARRELEELFHREMQKESRQFDGWDEYE

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=354, Percent_Identity=28.5310734463277, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1789749, Length=602, Percent_Identity=77.7408637873754, Blast_Score=916, Evalue=0.0,
Organism=Escherichia coli, GI1786232, Length=606, Percent_Identity=43.5643564356436, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1786685, Length=547, Percent_Identity=29.4332723948812, Blast_Score=177, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 66197; Mature: 66197

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGSTLLTAILLFLFAAVVTVPIARRLGIGAVLGYLIAGIAIGPWGLGFIRDVDEILHFS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ELGVVFLMFIIGLELNPSKLWELRRSIFGVGAGQVLITAAVLGALLYLTHFAWQAAVIGG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGLAMSSTAMALQLMREKGMNRNEGGQLGFSVLLFQDMAVIPALALIPILAGAGGSSDDW
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AQIALKVAAFGGMLIGGRFLLRPLFRYIASSGVREIFTAAALLVVLGAAMFMDALGLSMA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTFIAGVLLAESEYRHELEISIEPFKGLLLGLFFISVGMALNIGVLYTHLAEVLLGVVL
HHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVAIKSGVLYSLSRIFGLRSSVRLQFAGVLSQGGEFAFVLFSAAGTQKVLQPDQLALLLV
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHCCHHHHHHHHH
VVTLSMMTTPLLMQIIDRILVRRYNARDEDEETPYVEDDDPQVIIVGFGRFGQVIGRLLM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHH
ANKMRITVLERDVSAVGVLRRYGYKVYYGDATELELLRAAGAAKARSIVITCNEPEDTME
HCCCEEEEEHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCEEEEEECCCHHHHH
IVRLCQQHFPDLSILARARGRVEAHELLQAGVKQFSRETFSSALELGRKALMELGMHPHQ
HHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AYRAQQHFRRLDMRMLRELMPPHHGDVAQISRVKEARRELEELFHREMQKESRQFDGWDE
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YE
CC
>Mature Secondary Structure
MEGSTLLTAILLFLFAAVVTVPIARRLGIGAVLGYLIAGIAIGPWGLGFIRDVDEILHFS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ELGVVFLMFIIGLELNPSKLWELRRSIFGVGAGQVLITAAVLGALLYLTHFAWQAAVIGG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGLAMSSTAMALQLMREKGMNRNEGGQLGFSVLLFQDMAVIPALALIPILAGAGGSSDDW
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AQIALKVAAFGGMLIGGRFLLRPLFRYIASSGVREIFTAAALLVVLGAAMFMDALGLSMA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTFIAGVLLAESEYRHELEISIEPFKGLLLGLFFISVGMALNIGVLYTHLAEVLLGVVL
HHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVAIKSGVLYSLSRIFGLRSSVRLQFAGVLSQGGEFAFVLFSAAGTQKVLQPDQLALLLV
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHCCHHHHHHHHH
VVTLSMMTTPLLMQIIDRILVRRYNARDEDEETPYVEDDDPQVIIVGFGRFGQVIGRLLM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHH
ANKMRITVLERDVSAVGVLRRYGYKVYYGDATELELLRAAGAAKARSIVITCNEPEDTME
HCCCEEEEEHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCEEEEEECCCHHHHH
IVRLCQQHFPDLSILARARGRVEAHELLQAGVKQFSRETFSSALELGRKALMELGMHPHQ
HHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AYRAQQHFRRLDMRMLRELMPPHHGDVAQISRVKEARRELEELFHREMQKESRQFDGWDE
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA