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The map label for this gene is mscL

Identifier: 157372748

GI number: 157372748

Start: 5008214

End: 5008630

Strand: Direct

Name: mscL

Synonym: Spro_4515

Alternate gene names: 157372748

Gene position: 5008214-5008630 (Clockwise)

Preceding gene: 157372747

Following gene: 157372789

Centisome position: 91.91

GC content: 45.8

Gene sequence:

>417_bases
ATGAGTATGATGAAAGAGTTCCGCGAATTTGCCATGCGTGGCAACGTGGTTGATTTGGCAGTGGGTGTAATTATTGGTGC
GGCGTTTGGTAAGATCGTATCGTCTTTCGTTGCAGATATCATCATGCCACCGCTAGGCTTGCTGATCGGTGGTGTTGATT
TCAAACAATTCCACCTAGTATTACGCGAAGCTCAAGGCGCGGTTCCTGCAGTAGTGATGAACTACGGTTCATTCATCCAA
ACCATTTTCGATTTCGTGATTGTTGCCTTCGCTATTTTCCTGGCAATTAAATTAATGAATAAAGTACGCCGTAAACAGGA
AGAAGCGCCGGCAGCGCCGCCTGCGCCAACTGCAGAAGAAAAATTGCTGACTGAAATTCGCGATTTACTGAGCCAACAGC
AACAGCCAAAACTATAA

Upstream 100 bases:

>100_bases
TCTCTGCCATAGAAAGAGATAAAACTTTTCGCTCCTTTTGTGGCAAAGGGAAATTGCTTTGTTAAACTTGAGTTATTAAA
TTCTGCAAGGGGTGCGTTCT

Downstream 100 bases:

>100_bases
AATAGTTTATCTGGGATTACTAAAAAGCCATCTTATTCAGATGGCTTTTTTAATGCCTTAAAACCAGAAGGCCAGTGGTA
AATTATCCTTTGATAGTTTA

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 138; Mature: 137

Protein sequence:

>138_residues
MSMMKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSFVADIIMPPLGLLIGGVDFKQFHLVLREAQGAVPAVVMNYGSFIQ
TIFDFVIVAFAIFLAIKLMNKVRRKQEEAPAAPPAPTAEEKLLTEIRDLLSQQQQPKL

Sequences:

>Translated_138_residues
MSMMKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSFVADIIMPPLGLLIGGVDFKQFHLVLREAQGAVPAVVMNYGSFIQ
TIFDFVIVAFAIFLAIKLMNKVRRKQEEAPAAPPAPTAEEKLLTEIRDLLSQQQQPKL
>Mature_137_residues
SMMKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSFVADIIMPPLGLLIGGVDFKQFHLVLREAQGAVPAVVMNYGSFIQT
IFDFVIVAFAIFLAIKLMNKVRRKQEEAPAAPPAPTAEEKLLTEIRDLLSQQQQPKL

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=135, Percent_Identity=79.2592592592593, Blast_Score=215, Evalue=8e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_SERP5 (A8GKG9)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480737.1
- ProteinModelPortal:   A8GKG9
- SMR:   A8GKG9
- STRING:   A8GKG9
- GeneID:   5606039
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4515
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- ProtClustDB:   PRK00567
- BioCyc:   SPRO399741:SPRO_4515-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15177; Mature: 15046

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11e5c2e4)-; HASH(0x12089f7c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMMKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSFVADIIMPPLGLLIGGVDFKQFHLV
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LREAQGAVPAVVMNYGSFIQTIFDFVIVAFAIFLAIKLMNKVRRKQEEAPAAPPAPTAEE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
KLLTEIRDLLSQQQQPKL
HHHHHHHHHHHHHCCCCH
>Mature Secondary Structure 
SMMKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSFVADIIMPPLGLLIGGVDFKQFHLV
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LREAQGAVPAVVMNYGSFIQTIFDFVIVAFAIFLAIKLMNKVRRKQEEAPAAPPAPTAEE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
KLLTEIRDLLSQQQQPKL
HHHHHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA