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The map label for this gene is php [H]

Identifier: 157372726

GI number: 157372726

Start: 4979034

End: 4979927

Strand: Direct

Name: php [H]

Synonym: Spro_4493

Alternate gene names: 157372726

Gene position: 4979034-4979927 (Clockwise)

Preceding gene: 157372725

Following gene: 157372727

Centisome position: 91.38

GC content: 51.23

Gene sequence:

>894_bases
ATGGATCTTTCAACGCAATTCGATAGCCGTGGTTATACCTATGCGCACGAGCATCTGCATATCGACCTGTCGCCATTCAA
GCAGGATATGGATTGCAGGCTGGATTGTTACGCTTTGCTCGTCGATGAAATGAGGACGCTGGTCAGTAAAGGCGTTCTCA
ACCTGGTTGAGGTCACCAATCGCTATATGGGACGCAACCCGCAATTTATGCTGGATCTGATGCAAGAAAGCGGCATTAAC
ATCCTGGCGTCAACCGGATACTACCAACAGGAGTTCTACCCGCCACATGTCGCGCAACGTAGCGCGCAGCAATTGGCGCA
GGAAATGATCGACGAAATAGAACAGGGCATTGACGGCACAGAGTTACGTGCAAGCGTCATCGCAGAAATAGGCAGCAGTG
AAGGCACCATTACCCCGACGGAAGAAAAAGTGTTTCACGCCGCCGCATTGGCACATTTGGCGACCGGTAAACCTATTTCG
ACCCACACCAGTTTCAGCACCATGGGGCTGGAGCAGCTACGGCTATTAACCGGCTACGGGGTGGCCGCAGAGCATATTGT
TATCGGCCATTGCGATCTTCGCGATCAGTGGGAAACGTTACTACGCATTCTGGATACCGGTGCGTTAATTCAATTCGATA
CCATCGGGAAAAATAATTATTACCCAGACGAAAAACGTGTGGCTACCTTACTGGCACTCGCTCAACGGGGTTTATCAGGT
CAGGTCATGCTCTCCATGGATATCACCCGGCGCTCACACCTGGCGGCCAACGAAGGCCCAGGGTTCAGCTACCTCATTGA
TACGTTCATTCCCATGTTATTACAGGCGGGGATCCACCAGCGGGATATTGACCTTATGCTGCGCGATAACCCCAATACAT
TCTTCAAAGGATAA

Upstream 100 bases:

>100_bases
TGAGGCAACAGCAACGATGCAGACATTGCGGTGACACCTTTGGCGCCTCTGAACCACAAAGTGGTCATGGGTGTTTTTCA
CAACTCAACAAGGGGCAACA

Downstream 100 bases:

>100_bases
TAATAATGAAAAAAATCGGCATTGCAGGCTTACAACGTGAGCAAATAAAACAGGTTATTGAATCCACCGCACCCGGTAAA
TTTGAGTGCTTTATCCAAAA

Product: putative hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MDLSTQFDSRGYTYAHEHLHIDLSPFKQDMDCRLDCYALLVDEMRTLVSKGVLNLVEVTNRYMGRNPQFMLDLMQESGIN
ILASTGYYQQEFYPPHVAQRSAQQLAQEMIDEIEQGIDGTELRASVIAEIGSSEGTITPTEEKVFHAAALAHLATGKPIS
THTSFSTMGLEQLRLLTGYGVAAEHIVIGHCDLRDQWETLLRILDTGALIQFDTIGKNNYYPDEKRVATLLALAQRGLSG
QVMLSMDITRRSHLAANEGPGFSYLIDTFIPMLLQAGIHQRDIDLMLRDNPNTFFKG

Sequences:

>Translated_297_residues
MDLSTQFDSRGYTYAHEHLHIDLSPFKQDMDCRLDCYALLVDEMRTLVSKGVLNLVEVTNRYMGRNPQFMLDLMQESGIN
ILASTGYYQQEFYPPHVAQRSAQQLAQEMIDEIEQGIDGTELRASVIAEIGSSEGTITPTEEKVFHAAALAHLATGKPIS
THTSFSTMGLEQLRLLTGYGVAAEHIVIGHCDLRDQWETLLRILDTGALIQFDTIGKNNYYPDEKRVATLLALAQRGLSG
QVMLSMDITRRSHLAANEGPGFSYLIDTFIPMLLQAGIHQRDIDLMLRDNPNTFFKG
>Mature_297_residues
MDLSTQFDSRGYTYAHEHLHIDLSPFKQDMDCRLDCYALLVDEMRTLVSKGVLNLVEVTNRYMGRNPQFMLDLMQESGIN
ILASTGYYQQEFYPPHVAQRSAQQLAQEMIDEIEQGIDGTELRASVIAEIGSSEGTITPTEEKVFHAAALAHLATGKPIS
THTSFSTMGLEQLRLLTGYGVAAEHIVIGHCDLRDQWETLLRILDTGALIQFDTIGKNNYYPDEKRVATLLALAQRGLSG
QVMLSMDITRRSHLAANEGPGFSYLIDTFIPMLLQAGIHQRDIDLMLRDNPNTFFKG

Specific function: Its real enzymatic activity is not yet known. It was tested for general esterase, aminopeptidase, sulfatase, phosphatase, carbonic anhydrase, phosphodiesterase, and phosphotriesterase activities with the following substrates:P- nitrophenyl acetate, L-alan

COG id: COG1735

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphotriesterase family [H]

Homologues:

Organism=Homo sapiens, GI20070186, Length=267, Percent_Identity=26.9662921348315, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI47933341, Length=267, Percent_Identity=26.9662921348315, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1789780, Length=290, Percent_Identity=66.551724137931, Blast_Score=416, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24645325, Length=294, Percent_Identity=23.8095238095238, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017947
- InterPro:   IPR001559 [H]

Pfam domain/function: PF02126 PTE [H]

EC number: NA

Molecular weight: Translated: 33286; Mature: 33286

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS01323 PHOSPHOTRIESTERASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSTQFDSRGYTYAHEHLHIDLSPFKQDMDCRLDCYALLVDEMRTLVSKGVLNLVEVTN
CCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYMGRNPQFMLDLMQESGINILASTGYYQQEFYPPHVAQRSAQQLAQEMIDEIEQGIDGT
HHHCCCHHHHHHHHHHCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCH
ELRASVIAEIGSSEGTITPTEEKVFHAAALAHLATGKPISTHTSFSTMGLEQLRLLTGYG
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC
VAAEHIVIGHCDLRDQWETLLRILDTGALIQFDTIGKNNYYPDEKRVATLLALAQRGLSG
CEECEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCC
QVMLSMDITRRSHLAANEGPGFSYLIDTFIPMLLQAGIHQRDIDLMLRDNPNTFFKG
EEEEEEECHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MDLSTQFDSRGYTYAHEHLHIDLSPFKQDMDCRLDCYALLVDEMRTLVSKGVLNLVEVTN
CCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYMGRNPQFMLDLMQESGINILASTGYYQQEFYPPHVAQRSAQQLAQEMIDEIEQGIDGT
HHHCCCHHHHHHHHHHCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCH
ELRASVIAEIGSSEGTITPTEEKVFHAAALAHLATGKPISTHTSFSTMGLEQLRLLTGYG
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC
VAAEHIVIGHCDLRDQWETLLRILDTGALIQFDTIGKNNYYPDEKRVATLLALAQRGLSG
CEECEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCC
QVMLSMDITRRSHLAANEGPGFSYLIDTFIPMLLQAGIHQRDIDLMLRDNPNTFFKG
EEEEEEECHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9548740 [H]