Click here to switch to the map view.

The map label for this gene is mlaD [H]

Identifier: 157372591

GI number: 157372591

Start: 4829158

End: 4829709

Strand: Reverse

Name: mlaD [H]

Synonym: Spro_4358

Alternate gene names: 157372591

Gene position: 4829709-4829158 (Counterclockwise)

Preceding gene: 157372592

Following gene: 157372590

Centisome position: 88.64

GC content: 53.08

Gene sequence:

>552_bases
ATGCAAACGAAGAAGAGTGAAATCTGGGTTGGGGCATTTATGCTGATTGCGCTGTGCGCCGTCGTGTTTTTGTGCCTGCA
AGTGGCAAACATTAAGTCGATCGGTAACGAACCTACATACCGTATTTACGCGACCTTCGATAATATCGGTGGTTTGAAAG
CACGTTCACCGGTGAAAATCGGCGGCGTGGTGATTGGTCGAGTGGCCAATATCTCTCTCGATCCTAAAACTTATACGCCG
CGCGTTGCCCTGGATATCCAGGATAAATTCAACCAGATACCGGATACCAGCTCACTGGCGATCCGCACCTCCGGCCTGCT
GGGGGAGCAATATCTGGCACTGAACGTCGGTTTTGAAGACCCGGATATGGGCACCACCATTCTGAAGGACGGTGGTACCA
TTCAAGACACCAAATCAGCCATGGTTCTGGAAGATCTGATCGGCCAGTTCTTGTATAAGAGCGGCGGCCAGGATAACGCA
GATAAAGCGGGAGACGCGGCTGCCAGCCCGGCTGCCGGTACTGCGCCGCAACCTGCTGCCCCCATTCATTAA

Upstream 100 bases:

>100_bases
GGGATTAGCCGGGCAACGACCCGTACCGTGGTACATTCGTCACTGGCGGTGTTGGGATTGGATTTCGTGCTGACAGCACT
GATGTTTGGGAATTGATTCG

Downstream 100 bases:

>100_bases
GAGAGGAAGCCTGCATGTTTAAACGTTTACTTATGGTTGCCCTGCTGGTGGTTGCGCCGCTGGCGAACGCGGTCGATCAA
ACCAATCCTTACAGCCTGAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MQTKKSEIWVGAFMLIALCAVVFLCLQVANIKSIGNEPTYRIYATFDNIGGLKARSPVKIGGVVIGRVANISLDPKTYTP
RVALDIQDKFNQIPDTSSLAIRTSGLLGEQYLALNVGFEDPDMGTTILKDGGTIQDTKSAMVLEDLIGQFLYKSGGQDNA
DKAGDAAASPAAGTAPQPAAPIH

Sequences:

>Translated_183_residues
MQTKKSEIWVGAFMLIALCAVVFLCLQVANIKSIGNEPTYRIYATFDNIGGLKARSPVKIGGVVIGRVANISLDPKTYTP
RVALDIQDKFNQIPDTSSLAIRTSGLLGEQYLALNVGFEDPDMGTTILKDGGTIQDTKSAMVLEDLIGQFLYKSGGQDNA
DKAGDAAASPAAGTAPQPAAPIH
>Mature_183_residues
MQTKKSEIWVGAFMLIALCAVVFLCLQVANIKSIGNEPTYRIYATFDNIGGLKARSPVKIGGVVIGRVANISLDPKTYTP
RVALDIQDKFNQIPDTSSLAIRTSGLLGEQYLALNVGFEDPDMGTTILKDGGTIQDTKSAMVLEDLIGQFLYKSGGQDNA
DKAGDAAASPAAGTAPQPAAPIH

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG1463

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, periplasmic component

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein; Periplasmic side (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaD family [H]

Homologues:

Organism=Escherichia coli, GI1789584, Length=178, Percent_Identity=71.3483146067416, Blast_Score=257, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003399 [H]

Pfam domain/function: PF02470 MCE [H]

EC number: NA

Molecular weight: Translated: 19342; Mature: 19342

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTKKSEIWVGAFMLIALCAVVFLCLQVANIKSIGNEPTYRIYATFDNIGGLKARSPVKI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEE
GGVVIGRVANISLDPKTYTPRVALDIQDKFNQIPDTSSLAIRTSGLLGEQYLALNVGFED
CCEEEEEEEEEEECCCCCCCEEEEEEHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCC
PDMGTTILKDGGTIQDTKSAMVLEDLIGQFLYKSGGQDNADKAGDAAASPAAGTAPQPAA
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
PIH
CCC
>Mature Secondary Structure
MQTKKSEIWVGAFMLIALCAVVFLCLQVANIKSIGNEPTYRIYATFDNIGGLKARSPVKI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEE
GGVVIGRVANISLDPKTYTPRVALDIQDKFNQIPDTSSLAIRTSGLLGEQYLALNVGFED
CCEEEEEEEEEEECCCCCCCEEEEEEHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCC
PDMGTTILKDGGTIQDTKSAMVLEDLIGQFLYKSGGQDNADKAGDAAASPAAGTAPQPAA
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
PIH
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]