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The map label for this gene is dhaD [H]

Identifier: 157372506

GI number: 157372506

Start: 4738233

End: 4739339

Strand: Reverse

Name: dhaD [H]

Synonym: Spro_4273

Alternate gene names: 157372506

Gene position: 4739339-4738233 (Counterclockwise)

Preceding gene: 157372511

Following gene: 157372505

Centisome position: 86.98

GC content: 60.52

Gene sequence:

>1107_bases
ATGTTGAGAATCATCCAGTCTCCAGGCAAGTACATCCAGGGCGCCAACGCATTGGCCGCCGTGGGCGAATATGCGAAAGC
CCTTGCCGACCATTATTTCGTGATCGCCGACGACTTCGTGATGAAGCTGACGGGCGATACCCTGATGGGCAGCCTGCACC
AGCACGGGGTGAAGCATCACGCCTGTGTGTTCAACGGCGAATGCTGCCATAAGGAAATCGACCGGCTGGGCCAGGAACTG
AAGGCCCATGGCTGCCGCGGTGTGATTGGCGTCGGCGGCGGTAAAACGCTGGACACCGCCAAGGCCATCGCCCATTACCA
GCATCTGCCGGTGGTGCTGATCCCGACCATTGCTTCCACCGATGCGCCAACCAGCGCGCTGTCGGTGATCTACACCGAGC
AGGGCGAATTTGACGAATATCTGATCCACACCAGTAATCCGGACATGGTGATAATGGACAGCTCGATTATCGCCAAGGCA
CCGGTGCGGCTGCTGGTGGCAGGCATGGGCGACGCGCTTTCAACCTATTTTGAAGCACAGGCCTGTTTCGATGCCCAGGC
GACCAGCATGGCGGGCGGCAAGTCCACGCTGGCGGCATTGAGCCTGGCGCGGCTGTGCTATGAGACGCTGCTGGCAGAGG
GCGTGAAGGCCAAGCTGGCGGTTGAGGCCGGCGTGGTGACCGAGGCGGTCGAGCGCATTATCGAGGCCAATACCTATCTG
AGCGGCATCGGTTTCGAAAGCAGTGGCCTGGCGGCCGCGCATGCCATTCACAATGGTTTTACCGTGCTGGAAGAGTGCCA
TCACCTTTATCACGGCGAAAAGGTGGCGTTTGGCACGCTGTCACAGTTGGTGCTGCAAAACAGCAGCATGGCGCAGATCG
AAACGGTGCTGGATTTCTGTCAGCGTCTCGGCCTGCCAATTACGCTGGCGCAGATGGGCGTTAGCGGCGACCCGGCGGAG
AAAATCATGGCGGTGGCCGAAGCCAGCTGCGCGGCAGGCGAAACCACCCACAATATGCCGTTCGAGGTCACGCCTGCCAG
CGTACAGGCGGCCATTCTGACGGCAGACCGGCTGGGCAACGCCTGGCTGCAACAACACTCAAGCTGA

Upstream 100 bases:

>100_bases
CCCTATAGTCAGTCCATAACAACGCGACGGCACAAGACCGACGCACTATCACCTGATATCTGAACGTTGCCATCGCCAAT
TTCCCTACAAGGATCCGGTT

Downstream 100 bases:

>100_bases
TAGAGCAGGGGCGCAGCACGCTGCGTCCCGTTGGCCAAACGAGGAAATAACCGTGAAAAAACTGATTAACAAGGTTGAGT
CGGTACTGGAAGAGCAACTG

Product: iron-containing alcohol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MLRIIQSPGKYIQGANALAAVGEYAKALADHYFVIADDFVMKLTGDTLMGSLHQHGVKHHACVFNGECCHKEIDRLGQEL
KAHGCRGVIGVGGGKTLDTAKAIAHYQHLPVVLIPTIASTDAPTSALSVIYTEQGEFDEYLIHTSNPDMVIMDSSIIAKA
PVRLLVAGMGDALSTYFEAQACFDAQATSMAGGKSTLAALSLARLCYETLLAEGVKAKLAVEAGVVTEAVERIIEANTYL
SGIGFESSGLAAAHAIHNGFTVLEECHHLYHGEKVAFGTLSQLVLQNSSMAQIETVLDFCQRLGLPITLAQMGVSGDPAE
KIMAVAEASCAAGETTHNMPFEVTPASVQAAILTADRLGNAWLQQHSS

Sequences:

>Translated_368_residues
MLRIIQSPGKYIQGANALAAVGEYAKALADHYFVIADDFVMKLTGDTLMGSLHQHGVKHHACVFNGECCHKEIDRLGQEL
KAHGCRGVIGVGGGKTLDTAKAIAHYQHLPVVLIPTIASTDAPTSALSVIYTEQGEFDEYLIHTSNPDMVIMDSSIIAKA
PVRLLVAGMGDALSTYFEAQACFDAQATSMAGGKSTLAALSLARLCYETLLAEGVKAKLAVEAGVVTEAVERIIEANTYL
SGIGFESSGLAAAHAIHNGFTVLEECHHLYHGEKVAFGTLSQLVLQNSSMAQIETVLDFCQRLGLPITLAQMGVSGDPAE
KIMAVAEASCAAGETTHNMPFEVTPASVQAAILTADRLGNAWLQQHSS
>Mature_368_residues
MLRIIQSPGKYIQGANALAAVGEYAKALADHYFVIADDFVMKLTGDTLMGSLHQHGVKHHACVFNGECCHKEIDRLGQEL
KAHGCRGVIGVGGGKTLDTAKAIAHYQHLPVVLIPTIASTDAPTSALSVIYTEQGEFDEYLIHTSNPDMVIMDSSIIAKA
PVRLLVAGMGDALSTYFEAQACFDAQATSMAGGKSTLAALSLARLCYETLLAEGVKAKLAVEAGVVTEAVERIIEANTYL
SGIGFESSGLAAAHAIHNGFTVLEECHHLYHGEKVAFGTLSQLVLQNSSMAQIETVLDFCQRLGLPITLAQMGVSGDPAE
KIMAVAEASCAAGETTHNMPFEVTPASVQAAILTADRLGNAWLQQHSS

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. Exhibits a rather broad substrate specificity since it can also oxidize 1,2-prop

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=365, Percent_Identity=58.3561643835616, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1786815, Length=303, Percent_Identity=33.993399339934, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87082107, Length=298, Percent_Identity=27.1812080536913, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.6 [H]

Molecular weight: Translated: 38936; Mature: 38936

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2 ; PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIIQSPGKYIQGANALAAVGEYAKALADHYFVIADDFVMKLTGDTLMGSLHQHGVKHH
CCEEECCCCCHHCCCHHHHHHHHHHHHHHHCEEEEECCEEEEECCHHHHHHHHHCCCCCC
ACVFNGECCHKEIDRLGQELKAHGCRGVIGVGGGKTLDTAKAIAHYQHLPVVLIPTIAST
EEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCC
DAPTSALSVIYTEQGEFDEYLIHTSNPDMVIMDSSIIAKAPVRLLVAGMGDALSTYFEAQ
CCCCHHEEEEEECCCCCCCEEEECCCCCEEEECCCHHHHCCHHHHHHCCCHHHHHHHHHH
ACFDAQATSMAGGKSTLAALSLARLCYETLLAEGVKAKLAVEAGVVTEAVERIIEANTYL
HHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHH
SGIGFESSGLAAAHAIHNGFTVLEECHHLYHGEKVAFGTLSQLVLQNSSMAQIETVLDFC
CCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCHHHHHHHHHHH
QRLGLPITLAQMGVSGDPAEKIMAVAEASCAAGETTHNMPFEVTPASVQAAILTADRLGN
HHCCCCEEEHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHH
AWLQQHSS
HHHHCCCC
>Mature Secondary Structure
MLRIIQSPGKYIQGANALAAVGEYAKALADHYFVIADDFVMKLTGDTLMGSLHQHGVKHH
CCEEECCCCCHHCCCHHHHHHHHHHHHHHHCEEEEECCEEEEECCHHHHHHHHHCCCCCC
ACVFNGECCHKEIDRLGQELKAHGCRGVIGVGGGKTLDTAKAIAHYQHLPVVLIPTIAST
EEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCC
DAPTSALSVIYTEQGEFDEYLIHTSNPDMVIMDSSIIAKAPVRLLVAGMGDALSTYFEAQ
CCCCHHEEEEEECCCCCCCEEEECCCCCEEEECCCHHHHCCHHHHHHCCCHHHHHHHHHH
ACFDAQATSMAGGKSTLAALSLARLCYETLLAEGVKAKLAVEAGVVTEAVERIIEANTYL
HHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHH
SGIGFESSGLAAAHAIHNGFTVLEECHHLYHGEKVAFGTLSQLVLQNSSMAQIETVLDFC
CCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCHHHHHHHHHHH
QRLGLPITLAQMGVSGDPAEKIMAVAEASCAAGETTHNMPFEVTPASVQAAILTADRLGN
HHCCCCEEEHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHH
AWLQQHSS
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA