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The map label for this gene is dhaK [H]

Identifier: 157372505

GI number: 157372505

Start: 4737110

End: 4738180

Strand: Reverse

Name: dhaK [H]

Synonym: Spro_4272

Alternate gene names: 157372505

Gene position: 4738180-4737110 (Counterclockwise)

Preceding gene: 157372506

Following gene: 157372504

Centisome position: 86.96

GC content: 60.32

Gene sequence:

>1071_bases
GTGAAAAAACTGATTAACAAGGTTGAGTCGGTACTGGAAGAGCAACTGCTGGGGTTGGCAGAGGCTCATCCTGAGCTGTT
GGTGCATCAGGAGCCGGTGTTTGTCACCCGCGCCGATGCGCCGGTAGCGGGCAAGGTGGCGATCCTGTCCGGCGGCGGCA
GCGGCCACGAGCCGATGCACTGCGGTTTTGTCGGCGAAGGCATGCTCGACGGCGCCTGCCCGGGGGAAATTTTTACCTCG
CCAACGCCGGATAAAATGTACGAGTGCGGCCAGGCGATCGACGGCGGCGCCGGGGTATTACTGCTGATTAAAAACTACAC
CGGCGACGTACTGAACTTTGAAACCGCCACTGAGCTGCTGCATGACAGCGGTGTCCAGGTGGCGACGGTGCTGGTGGATG
ATGACGTGGCGGTTAAAGACAGCCTGTTCACCGCCGGGCGACGCGGGGTGGCCAATACCGTGCTGATGGAGAAACTGCTC
GGTGCGGCAACGGCGCGTGGTGATGACCTCGATAGCATCGTGACGCTGGGCCACAGCATCAATAATCACGGGCATTCGAT
CGGTATCGCGCTGGGGGCCTGTACCGTACCGGCTGCCGGTAAACCGTCATTCGTGTTGGCGGAAAACGAGATGGAATTCG
GCGTCGGTATTCACGGTGAACCGGGGATTGCGCGGCGCCCGTTCACCACCCTGAATGCGGCGGTGGACGACATGTTCCAT
ACCCTGATCGACCATGGTCATTACCAGCGCACCATCCGCGTCTGGGATCGTCAACAGGGCGAATGGCGTGATGAAGTGCA
GACCAAACAACCGCTGACGCGCGGTGACCGGGTGATCGCACTGGTAAACAACCTGGGTGCGACGCCGCTGTCCGAACTGT
ATGGCGTCTATCACCGGCTGGCGGAGCGTTGTGCCGAGGCGGGCATGATTGTTGAGCGCAGCCTGGTGGGTTCCTATTGC
ACATCGCTGGATATGCAAGGGGTCTCAATCACGCTGCTGAAAGTGGACGATGAACTGTTGTCCCTGTGGGATGCGCCGGT
GAAAACGCCTGCGCTGCGTTGGGGTTGCTGA

Upstream 100 bases:

>100_bases
ACGGCAGACCGGCTGGGCAACGCCTGGCTGCAACAACACTCAAGCTGATAGAGCAGGGGCGCAGCACGCTGCGTCCCGTT
GGCCAAACGAGGAAATAACC

Downstream 100 bases:

>100_bases
GGAGGAATCATGGCGTTAACCAAACAACAAGTGGTCGACTGGCTGATGCGCTGCGGCGAGGTATTTACTCAGCAGCGTGA
TTTTTTAACCCGGCTGGATA

Product: dihydroxyacetone kinase subunit DhaK

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MKKLINKVESVLEEQLLGLAEAHPELLVHQEPVFVTRADAPVAGKVAILSGGGSGHEPMHCGFVGEGMLDGACPGEIFTS
PTPDKMYECGQAIDGGAGVLLLIKNYTGDVLNFETATELLHDSGVQVATVLVDDDVAVKDSLFTAGRRGVANTVLMEKLL
GAATARGDDLDSIVTLGHSINNHGHSIGIALGACTVPAAGKPSFVLAENEMEFGVGIHGEPGIARRPFTTLNAAVDDMFH
TLIDHGHYQRTIRVWDRQQGEWRDEVQTKQPLTRGDRVIALVNNLGATPLSELYGVYHRLAERCAEAGMIVERSLVGSYC
TSLDMQGVSITLLKVDDELLSLWDAPVKTPALRWGC

Sequences:

>Translated_356_residues
MKKLINKVESVLEEQLLGLAEAHPELLVHQEPVFVTRADAPVAGKVAILSGGGSGHEPMHCGFVGEGMLDGACPGEIFTS
PTPDKMYECGQAIDGGAGVLLLIKNYTGDVLNFETATELLHDSGVQVATVLVDDDVAVKDSLFTAGRRGVANTVLMEKLL
GAATARGDDLDSIVTLGHSINNHGHSIGIALGACTVPAAGKPSFVLAENEMEFGVGIHGEPGIARRPFTTLNAAVDDMFH
TLIDHGHYQRTIRVWDRQQGEWRDEVQTKQPLTRGDRVIALVNNLGATPLSELYGVYHRLAERCAEAGMIVERSLVGSYC
TSLDMQGVSITLLKVDDELLSLWDAPVKTPALRWGC
>Mature_356_residues
MKKLINKVESVLEEQLLGLAEAHPELLVHQEPVFVTRADAPVAGKVAILSGGGSGHEPMHCGFVGEGMLDGACPGEIFTS
PTPDKMYECGQAIDGGAGVLLLIKNYTGDVLNFETATELLHDSGVQVATVLVDDDVAVKDSLFTAGRRGVANTVLMEKLL
GAATARGDDLDSIVTLGHSINNHGHSIGIALGACTVPAAGKPSFVLAENEMEFGVGIHGEPGIARRPFTTLNAAVDDMFH
TLIDHGHYQRTIRVWDRQQGEWRDEVQTKQPLTRGDRVIALVNNLGATPLSELYGVYHRLAERCAEAGMIVERSLVGSYC
TSLDMQGVSITLLKVDDELLSLWDAPVKTPALRWGC

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=357, Percent_Identity=37.8151260504202, Blast_Score=206, Evalue=4e-53,
Organism=Escherichia coli, GI87081857, Length=355, Percent_Identity=76.3380281690141, Blast_Score=583, Evalue=1e-168,
Organism=Caenorhabditis elegans, GI17565018, Length=350, Percent_Identity=33.4285714285714, Blast_Score=188, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6321055, Length=356, Percent_Identity=35.9550561797753, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6323570, Length=331, Percent_Identity=34.1389728096677, Blast_Score=158, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 38140; Mature: 38140

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLINKVESVLEEQLLGLAEAHPELLVHQEPVFVTRADAPVAGKVAILSGGGSGHEPMH
CHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCEEEEECCCCCCCEEEEEECCCCCCCCEE
CGFVGEGMLDGACPGEIFTSPTPDKMYECGQAIDGGAGVLLLIKNYTGDVLNFETATELL
ECCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCEECHHHHHHHH
HDSGVQVATVLVDDDVAVKDSLFTAGRRGVANTVLMEKLLGAATARGDDLDSIVTLGHSI
HCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
NNHGHSIGIALGACTVPAAGKPSFVLAENEMEFGVGIHGEPGIARRPFTTLNAAVDDMFH
CCCCCEEEEEEEEEECCCCCCCCEEEECCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHH
TLIDHGHYQRTIRVWDRQQGEWRDEVQTKQPLTRGDRVIALVNNLGATPLSELYGVYHRL
HHHHCCCCEEHHEECCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
AERCAEAGMIVERSLVGSYCTSLDMQGVSITLLKVDDELLSLWDAPVKTPALRWGC
HHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKKLINKVESVLEEQLLGLAEAHPELLVHQEPVFVTRADAPVAGKVAILSGGGSGHEPMH
CHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCEEEEECCCCCCCEEEEEECCCCCCCCEE
CGFVGEGMLDGACPGEIFTSPTPDKMYECGQAIDGGAGVLLLIKNYTGDVLNFETATELL
ECCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCEECHHHHHHHH
HDSGVQVATVLVDDDVAVKDSLFTAGRRGVANTVLMEKLLGAATARGDDLDSIVTLGHSI
HCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
NNHGHSIGIALGACTVPAAGKPSFVLAENEMEFGVGIHGEPGIARRPFTTLNAAVDDMFH
CCCCCEEEEEEEEEECCCCCCCCEEEECCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHH
TLIDHGHYQRTIRVWDRQQGEWRDEVQTKQPLTRGDRVIALVNNLGATPLSELYGVYHRL
HHHHCCCCEEHHEECCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
AERCAEAGMIVERSLVGSYCTSLDMQGVSITLLKVDDELLSLWDAPVKTPALRWGC
HHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]