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The map label for this gene is xapA [H]

Identifier: 157372488

GI number: 157372488

Start: 4717768

End: 4718625

Strand: Reverse

Name: xapA [H]

Synonym: Spro_4255

Alternate gene names: 157372488

Gene position: 4718625-4717768 (Counterclockwise)

Preceding gene: 157372490

Following gene: 157372487

Centisome position: 86.6

GC content: 60.84

Gene sequence:

>858_bases
ATGGGGATAACCTCCCCCCTCATAGAAGAGAATACAATGTTTAATCAAGAAGATGCTTTTTCCTGTGCCGAAGTGATCCG
CACCAGGCTGCCGGACTTCCAGCCGCACGCTGCTTTGATACTGGGCTCCGGGCTGGGCGCGCTGGCAGAGGTGATGACCG
ACACCCTGACCATCGACTACGCAGAGCTGCCGGGTTTCCCGGTCAGTGGCGTGGTGGGCCATGCCGGGCAACTGGTTACC
GGTTGGCTGGAGGGCGTGCCGGTGCTCTGCATGAAAGGGCGCGGTCACTACTACGAAGGGCGCGGCATGCAGGTGATGAC
CACCGCTGTGCGCACCTTCAAACTGCTGGGCTGCGAATTCCTGCTGGCCACCAATGCTGCCGGCTCGCTGCGTCAGTCGG
TGGCACCGGGCAGCCTGGTGGCACTCACCGACCACATTAATTTTATGCCTGAGTCGCCGCTGGTTGGCGATAACGACGAG
CGTTTTGGCCCGCGTTTCTTCAGCCTGGCCAACGCCTACGATCGCGAGCTGCGCGGCCAGTTGGCACAGGTGGCGCAAAG
CGTAGGCATTCCGCTGGCCGAAGGGGTATTTGCCGCTTACACCGGGCCAAACTTCGAAACCCCGGCGGAAATCCGCATGA
TGCAAACGCTGGGCTGCGACGTGGTCGGTATGTCTATCGTGCCGGAAGTGCTCTCGGCACGTCACTGTGGGCTGAAAGTG
CTGGTAGTGTCCGCGATGACCAACTACGCCGAAGGGCTGTCCGATACGCCGCTGTCCCATGAGCAAACCCTGAGCTGTGC
GGCGTTGGCGGCAGAGGACTTTATGCGCCTGATCCGCGAATTCTTCAAAACGCGGTAA

Upstream 100 bases:

>100_bases
TGATGCTGATGATCGCCTCCTGGAGCTACCAATTTATAGGTAAATGGAATAAATCGGCCGCCAATAATGTATTGGTCGCC
GCCGCGCCTCGCTCTCTACT

Downstream 100 bases:

>100_bases
TGCCGTAAACCTCCCGACGGCAGGGTGCAGGGGCCCTGTCGTGCGAATCATCGTTTTAATCAGAATAATAAGGTGATGGT
GATGGCAATAAAAACGCGAC

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MGITSPLIEENTMFNQEDAFSCAEVIRTRLPDFQPHAALILGSGLGALAEVMTDTLTIDYAELPGFPVSGVVGHAGQLVT
GWLEGVPVLCMKGRGHYYEGRGMQVMTTAVRTFKLLGCEFLLATNAAGSLRQSVAPGSLVALTDHINFMPESPLVGDNDE
RFGPRFFSLANAYDRELRGQLAQVAQSVGIPLAEGVFAAYTGPNFETPAEIRMMQTLGCDVVGMSIVPEVLSARHCGLKV
LVVSAMTNYAEGLSDTPLSHEQTLSCAALAAEDFMRLIREFFKTR

Sequences:

>Translated_285_residues
MGITSPLIEENTMFNQEDAFSCAEVIRTRLPDFQPHAALILGSGLGALAEVMTDTLTIDYAELPGFPVSGVVGHAGQLVT
GWLEGVPVLCMKGRGHYYEGRGMQVMTTAVRTFKLLGCEFLLATNAAGSLRQSVAPGSLVALTDHINFMPESPLVGDNDE
RFGPRFFSLANAYDRELRGQLAQVAQSVGIPLAEGVFAAYTGPNFETPAEIRMMQTLGCDVVGMSIVPEVLSARHCGLKV
LVVSAMTNYAEGLSDTPLSHEQTLSCAALAAEDFMRLIREFFKTR
>Mature_284_residues
GITSPLIEENTMFNQEDAFSCAEVIRTRLPDFQPHAALILGSGLGALAEVMTDTLTIDYAELPGFPVSGVVGHAGQLVTG
WLEGVPVLCMKGRGHYYEGRGMQVMTTAVRTFKLLGCEFLLATNAAGSLRQSVAPGSLVALTDHINFMPESPLVGDNDER
FGPRFFSLANAYDRELRGQLAQVAQSVGIPLAEGVFAAYTGPNFETPAEIRMMQTLGCDVVGMSIVPEVLSARHCGLKVL
VVSAMTNYAEGLSDTPLSHEQTLSCAALAAEDFMRLIREFFKTR

Specific function: The nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the nucleoside molecule, with the formation of the corresponding free bases and pentose-1-phosphate. This protein can degrade all purine nucleosides except ade

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=274, Percent_Identity=41.970802919708, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI47132622, Length=176, Percent_Identity=28.4090909090909, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1788746, Length=262, Percent_Identity=66.030534351145, Blast_Score=359, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17541190, Length=277, Percent_Identity=38.6281588447653, Blast_Score=188, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6323238, Length=271, Percent_Identity=38.0073800738007, Blast_Score=167, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24656090, Length=287, Percent_Identity=40.418118466899, Blast_Score=207, Evalue=5e-54,
Organism=Drosophila melanogaster, GI24656093, Length=268, Percent_Identity=41.4179104477612, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI45552887, Length=268, Percent_Identity=41.4179104477612, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI45552885, Length=268, Percent_Identity=41.4179104477612, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24762376, Length=223, Percent_Identity=34.0807174887892, Blast_Score=125, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR010943
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: 2.4.2.-

Molecular weight: Translated: 30711; Mature: 30579

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS01240 PNP_MTAP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGITSPLIEENTMFNQEDAFSCAEVIRTRLPDFQPHAALILGSGLGALAEVMTDTLTIDY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHEEEH
AELPGFPVSGVVGHAGQLVTGWLEGVPVLCMKGRGHYYEGRGMQVMTTAVRTFKLLGCEF
HHCCCCCCHHHHHCCHHHHHHHHCCCCEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHH
LLATNAAGSLRQSVAPGSLVALTDHINFMPESPLVGDNDERFGPRFFSLANAYDRELRGQ
EEECCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LAQVAQSVGIPLAEGVFAAYTGPNFETPAEIRMMQTLGCDVVGMSIVPEVLSARHCGLKV
HHHHHHHHCCCHHHCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHH
LVVSAMTNYAEGLSDTPLSHEQTLSCAALAAEDFMRLIREFFKTR
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GITSPLIEENTMFNQEDAFSCAEVIRTRLPDFQPHAALILGSGLGALAEVMTDTLTIDY
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHEEEH
AELPGFPVSGVVGHAGQLVTGWLEGVPVLCMKGRGHYYEGRGMQVMTTAVRTFKLLGCEF
HHCCCCCCHHHHHCCHHHHHHHHCCCCEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHH
LLATNAAGSLRQSVAPGSLVALTDHINFMPESPLVGDNDERFGPRFFSLANAYDRELRGQ
EEECCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LAQVAQSVGIPLAEGVFAAYTGPNFETPAEIRMMQTLGCDVVGMSIVPEVLSARHCGLKV
HHHHHHHHCCCHHHCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHH
LVVSAMTNYAEGLSDTPLSHEQTLSCAALAAEDFMRLIREFFKTR
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559336; 9205837; 9278503 [H]