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The map label for this gene is outE [H]

Identifier: 157372483

GI number: 157372483

Start: 4711517

End: 4713001

Strand: Reverse

Name: outE [H]

Synonym: Spro_4250

Alternate gene names: 157372483

Gene position: 4713001-4711517 (Counterclockwise)

Preceding gene: 157372484

Following gene: 157372482

Centisome position: 86.5

GC content: 56.97

Gene sequence:

>1485_bases
GTGAACACACCCTGCTTGTCTTATGCCTGGGCCCGTCAGCATCACGCAGTACTCACCAGCGAAGAAAACCAATACACCCT
ATGGTGTCGACAGGACGTGGGGCTGGCCACGTTGGCCGAGCTTGCGCGTCTGCTGGAGGCCGACATTCCGCTGTGTTTGC
TGGACGAAAATGCCTTTGAGCAGAAGTTGCTGGAACATTACCAACAGGGCAACAGCAATACGCAAAAGACCATGGACGAC
CTGGGTAAGGAGCTTGATTTCTACAGCCTGGCGCAGGCGCTGCCGGCCACCCAGGATCTGCTTGATGAACAGGACGATGC
GCCGATTATCCGTTTGCTTAATGCGATGCTCAGTGAAGCCATTAAAGAGCGGGCCTCAGATATTCATATCGAGACCTATG
ACAGCGAACTGGTGATCCGCTTTCGTATTGATGGGGTGCTGAAAACCATTCTGACTCCCCAGCATCAACTGGCGGCCTTG
CTGACATCTCGCATTAAGGTGATGTCCAAACTGGACATTGCCGAGAAGCGCATCCCGCAGGATGGCCGCATCACGTTGAA
GATTGGCGGCCGAGCCGTCGATGTGCGGGTGTCCGTACTTCCGACCAATCATGGTGAGCGGGTGGTGATGCGTCTGCTGG
ATAAAAGCAGCGTCAGTCTGGATCTCGCCAGGATGGGCATACGCTCGGTGGAGTTCGCGGCGCTGACGCGACTGGTTCAT
CAGCCCCATGGGGTGATCCTGGTGACCGGCCCGACCGGCTCGGGCAAAAGCACCACGCTTTATGCGTTACTGATGCAGAT
TAATGACGGCAATCGCAACATCATGACCATAGAAGACCCGGTGGAGTTTGAATTAAAGGGCATTTCGCAAACCCAGGTCA
ACACGCGGGTCGATATGACGTTTGCCCGCGGGTTACGCGCCCTGCTGCGGCAAGATCCGGATGTGGTGTTGCTGGGCGAA
ATACGCGATACCGAAACCGCACATATCGCCGTACAGGCTTCACTGACCGGGCACTTGGTGTTGTCCACGCTGCATACCAA
TACCGCCATCGGCGCCATCACCCGCCTGCGCGATATGGGGATCGAACCCTTCCTGCTGGCCAGTTCGCTGACGGGCATTC
TCGCTCAGCGGCTGGTGCGAAAACTGTGCCCGCATTGCCGCCAGGCCTATGTGCTTTCTGCAGAGCAAGCGCTCTCGCTA
CAGCTCTCTGTCGAGATTGAGCAGGTCGCGTGGCGTCCGATCGGCTGTGAAAAATGCAATGGCCTGGGCTACCGTGGGCG
TCTGGGGATCCATGAACTGCTGCTGATTGATGACGCCATCCGCAAAGCCATTCATTTGGGCAGCAGTGAGCAGGAGATAG
AACAATTGTGCGGCCATGCGCGACACACCCTGCGGCAAGATGGTGCGCGCAAACTGCTGACCGGTGAAACCAGTATTGAG
GAAGTTCTGCGGGTCACCGGGGGGGATCATTGCGGTGAAGTTTAA

Upstream 100 bases:

>100_bases
CCCATGCGGAGGCGCAGCTCGATAGGGTCGGTTCCACTGAGAATGGCACTTTCCAGCGGATCCAACGGCAAATTGATGCA
TTTTACCCGCGGGGGGAGCT

Downstream 100 bases:

>100_bases
CTATCACGCCTACGATGCCGCCGGCAAAGGCGTCAGAGGGGTACTGGAGACGGATTCTTTGCGCAGTGCCCGGCAACGGC
TCCGTGAGCAAAACCTGACG

Product: general secretory pathway protein E

Products: NA

Alternate protein names: Pectic enzymes secretion protein outE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MNTPCLSYAWARQHHAVLTSEENQYTLWCRQDVGLATLAELARLLEADIPLCLLDENAFEQKLLEHYQQGNSNTQKTMDD
LGKELDFYSLAQALPATQDLLDEQDDAPIIRLLNAMLSEAIKERASDIHIETYDSELVIRFRIDGVLKTILTPQHQLAAL
LTSRIKVMSKLDIAEKRIPQDGRITLKIGGRAVDVRVSVLPTNHGERVVMRLLDKSSVSLDLARMGIRSVEFAALTRLVH
QPHGVILVTGPTGSGKSTTLYALLMQINDGNRNIMTIEDPVEFELKGISQTQVNTRVDMTFARGLRALLRQDPDVVLLGE
IRDTETAHIAVQASLTGHLVLSTLHTNTAIGAITRLRDMGIEPFLLASSLTGILAQRLVRKLCPHCRQAYVLSAEQALSL
QLSVEIEQVAWRPIGCEKCNGLGYRGRLGIHELLLIDDAIRKAIHLGSSEQEIEQLCGHARHTLRQDGARKLLTGETSIE
EVLRVTGGDHCGEV

Sequences:

>Translated_494_residues
MNTPCLSYAWARQHHAVLTSEENQYTLWCRQDVGLATLAELARLLEADIPLCLLDENAFEQKLLEHYQQGNSNTQKTMDD
LGKELDFYSLAQALPATQDLLDEQDDAPIIRLLNAMLSEAIKERASDIHIETYDSELVIRFRIDGVLKTILTPQHQLAAL
LTSRIKVMSKLDIAEKRIPQDGRITLKIGGRAVDVRVSVLPTNHGERVVMRLLDKSSVSLDLARMGIRSVEFAALTRLVH
QPHGVILVTGPTGSGKSTTLYALLMQINDGNRNIMTIEDPVEFELKGISQTQVNTRVDMTFARGLRALLRQDPDVVLLGE
IRDTETAHIAVQASLTGHLVLSTLHTNTAIGAITRLRDMGIEPFLLASSLTGILAQRLVRKLCPHCRQAYVLSAEQALSL
QLSVEIEQVAWRPIGCEKCNGLGYRGRLGIHELLLIDDAIRKAIHLGSSEQEIEQLCGHARHTLRQDGARKLLTGETSIE
EVLRVTGGDHCGEV
>Mature_494_residues
MNTPCLSYAWARQHHAVLTSEENQYTLWCRQDVGLATLAELARLLEADIPLCLLDENAFEQKLLEHYQQGNSNTQKTMDD
LGKELDFYSLAQALPATQDLLDEQDDAPIIRLLNAMLSEAIKERASDIHIETYDSELVIRFRIDGVLKTILTPQHQLAAL
LTSRIKVMSKLDIAEKRIPQDGRITLKIGGRAVDVRVSVLPTNHGERVVMRLLDKSSVSLDLARMGIRSVEFAALTRLVH
QPHGVILVTGPTGSGKSTTLYALLMQINDGNRNIMTIEDPVEFELKGISQTQVNTRVDMTFARGLRALLRQDPDVVLLGE
IRDTETAHIAVQASLTGHLVLSTLHTNTAIGAITRLRDMGIEPFLLASSLTGILAQRLVRKLCPHCRQAYVLSAEQALSL
QLSVEIEQVAWRPIGCEKCNGLGYRGRLGIHELLLIDDAIRKAIHLGSSEQEIEQLCGHARHTLRQDGARKLLTGETSIE
EVLRVTGGDHCGEV

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pectate lyases and cellulases across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=435, Percent_Identity=58.3908045977011, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1786296, Length=395, Percent_Identity=43.7974683544304, Blast_Score=324, Evalue=1e-89,
Organism=Escherichia coli, GI87082188, Length=195, Percent_Identity=43.5897435897436, Blast_Score=125, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 54785; Mature: 54785

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPCLSYAWARQHHAVLTSEENQYTLWCRQDVGLATLAELARLLEADIPLCLLDENAFE
CCCCCCCHHHHHHCCEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCHHH
QKLLEHYQQGNSNTQKTMDDLGKELDFYSLAQALPATQDLLDEQDDAPIIRLLNAMLSEA
HHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHH
IKERASDIHIETYDSELVIRFRIDGVLKTILTPQHQLAALLTSRIKVMSKLDIAEKRIPQ
HHHHHCCCEEEEECCEEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
DGRITLKIGGRAVDVRVSVLPTNHGERVVMRLLDKSSVSLDLARMGIRSVEFAALTRLVH
CCEEEEEECCEEEEEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHH
QPHGVILVTGPTGSGKSTTLYALLMQINDGNRNIMTIEDPVEFELKGISQTQVNTRVDMT
CCCCEEEEECCCCCCCCCEEEEEEEEEECCCCEEEEECCCCCEEECCCCHHHCCCEEHHH
FARGLRALLRQDPDVVLLGEIRDTETAHIAVQASLTGHLVLSTLHTNTAIGAITRLRDMG
HHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCEEEEEEHHCCHHHHHHHHHHHCC
IEPFLLASSLTGILAQRLVRKLCPHCRQAYVLSAEQALSLQLSVEIEQVAWRPIGCEKCN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEEEEEEHHHCCCCCCCHHCC
GLGYRGRLGIHELLLIDDAIRKAIHLGSSEQEIEQLCGHARHTLRQDGARKLLTGETSIE
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCHHHH
EVLRVTGGDHCGEV
HHHHHCCCCCCCCC
>Mature Secondary Structure
MNTPCLSYAWARQHHAVLTSEENQYTLWCRQDVGLATLAELARLLEADIPLCLLDENAFE
CCCCCCCHHHHHHCCEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCHHH
QKLLEHYQQGNSNTQKTMDDLGKELDFYSLAQALPATQDLLDEQDDAPIIRLLNAMLSEA
HHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHH
IKERASDIHIETYDSELVIRFRIDGVLKTILTPQHQLAALLTSRIKVMSKLDIAEKRIPQ
HHHHHCCCEEEEECCEEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
DGRITLKIGGRAVDVRVSVLPTNHGERVVMRLLDKSSVSLDLARMGIRSVEFAALTRLVH
CCEEEEEECCEEEEEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHH
QPHGVILVTGPTGSGKSTTLYALLMQINDGNRNIMTIEDPVEFELKGISQTQVNTRVDMT
CCCCEEEEECCCCCCCCCEEEEEEEEEECCCCEEEEECCCCCEEECCCCHHHCCCEEHHH
FARGLRALLRQDPDVVLLGEIRDTETAHIAVQASLTGHLVLSTLHTNTAIGAITRLRDMG
HHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCEEEEEEHHCCHHHHHHHHHHHCC
IEPFLLASSLTGILAQRLVRKLCPHCRQAYVLSAEQALSLQLSVEIEQVAWRPIGCEKCN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEEEEEEHHHCCCCCCCHHCC
GLGYRGRLGIHELLLIDDAIRKAIHLGSSEQEIEQLCGHARHTLRQDGARKLLTGETSIE
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCHHHH
EVLRVTGGDHCGEV
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]