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The map label for this gene is pulD [H]

Identifier: 157372484

GI number: 157372484

Start: 4712998

End: 4714980

Strand: Reverse

Name: pulD [H]

Synonym: Spro_4251

Alternate gene names: 157372484

Gene position: 4714980-4712998 (Counterclockwise)

Preceding gene: 157372485

Following gene: 157372483

Centisome position: 86.53

GC content: 53.61

Gene sequence:

>1983_bases
ATGTACAACCTCGACTTATTTCCAGGCAGACATTGGTACATGCACGCAAAAATGACAAATATGCTCTTCTCGAAAATGAT
CGCATTAATGGCAGTGCTCGTCATGCTGCTGTGGGTGAGCGCGCCCGTTCAGGCCGAGGCGTTTTCCGCCAATTTTAAAG
GAACGGACATACACGAATTTATCAGTACAGCCAGTAAGGCGCTGGGGAAAACCATCATTATTGACGATGCGGTAAAAGGC
AAGGTTACCGTGCGCAGTTATGAGCCGCTGGATGCCGGGCAATATTACCAATTTTTCCTCAACGTGCTTGATGTCTATGG
TTATGCGGTGATCAGCATGCCCAACAACGTTCTGAAGGTGATCCCGGCGAAAGACGGCAAAAGGGCGGCGCTCAACGCCC
TGAATACCCCAGCCGAAGGCGATGAGCTGATCATGCGGATTGTGCCCCTGAACAATATCGCGGCGAAAGAGGTGGCGCCG
TTGATGCGCCAGTTTAACGACAGCATTGGTATCGGGAACGTGGTGTTCCTGGAAAGTGGCAATGCGTTGTTGATCACCGG
CCGTGCCGATGTAGTCAATAGTCTGGTTGAGCTGGCCCATGAAATGGATCGTACCGAACAGGCCGGAGTGGTCTCTGTCG
CCTTGCAACATGCCTCCGCCACCGATGTGGTCAGCATTGTCACCACGTTATTTCGGGATGAAAACCGCACGAAAAAGGGC
GCGCTTCCCGGCATGAAAGTGGTTGCCGATGAACGGACCAACACCGTGCTGATTGCCGGCGAACCGAGCCTGCAGGCGCG
CATCGAAGGGATTATCCAGCAGTTGGATCAAGAAAAAGCCAGCCAGGGGAATACCAAGGTGATCCGGCTGAAATATGCCA
AAGCGCAAGGCCTGCTTGAAGTGTTGTCCGGCGTCAGCGCCAATCTGCAAGACGAGAAAAAAACGGTCGGCAACAACGTT
TCCGTCATGAAGAACATGGTGATCAAGGCAGATGAACAGACCAATGCGCTGATCATCAACGCCACACCGGACGTGATGAG
CAACCTGGAAAAAGTCATTGAGCAGCTTGATATTCGCCGGGCTCAGGTACTGGTTGAAGCGGTGATTGTCGAAATGCAAA
ACGCAGACGGTCTGAACCTGGGTATCCAATGGGCCAACCGTTACGGTGGCGGTACGCAGTTCGGCAATGCTCAGGCGCAG
ATCGCTCCCGGCTTTAAAGGGGGAATGACGGAGGTGTTTAAAAACGCCAACGGTCTGGTCACGGGATTTTACAGCGGTAA
CTGGGCCGGCTTGCTCACCGCCATCAGTAACCAGAGCCAGAACAATATTCTCGCCACGCCGAGCATCATGACGTTGGACA
ACGAAGATGCCGAGTTTAGCGTGGGTCAGGATGTGCCAATTCTGACCGGCTCGCAAACCACCAACAGTGACAACGTGTTT
AATACGGTGGCGCGCAAAACCGTCGGCATCAAGCTCAAGGTCAAGCCGCAAATCAACAAAGGGCAGTCGGTACTGCTGCA
GATCGAACAGGAAGTTTCCAGCGTGGCGGAAAACAGCTCGGTGGATAAGGATAACCTGGGCGCGACTTTCAATATCCGGA
CGGTGAATAACACGGTGCTGGTTGAAAGTGGTGAGACCGTGGTGGTCGGCGGTTTGCTGGACAAGTCGCAATCTGACAGC
AAAAGTGCGGTGCCGTTTTTGGAACGCATACCGCTGCTTGGCGCTTTGTTCCGCTCTACTTCTCATAAAGAGAGCAAACG
GAATCTTATCTTGTTTATTCGCCCGACGGTCATACGCTCGACCGAGGAATACGCGCAAGAGAGTATGCGCAAAGCGGCCC
GCTTCCGATCACTCGACGCTCAGCCTCTCCCGTTAAAAACCCATGCGGAGGCGCAGCTCGATAGGGTCGGTTCCACTGAG
AATGGCACTTTCCAGCGGATCCAACGGCAAATTGATGCATTTTACCCGCGGGGGGAGCTGTGA

Upstream 100 bases:

>100_bases
GATCATTATCAATGAAAACGGATTTTATGCCGCATTAATGCTCGAAAATTGAGTGCAATCACAGCGGTTGCATGACAGCA
GGCCGCAATCGGATACAGAT

Downstream 100 bases:

>100_bases
ACACACCCTGCTTGTCTTATGCCTGGGCCCGTCAGCATCACGCAGTACTCACCAGCGAAGAAAACCAATACACCCTATGG
TGTCGACAGGACGTGGGGCT

Product: general secretion pathway protein D

Products: NA

Alternate protein names: Pullulanase secretion envelope pulD [H]

Number of amino acids: Translated: 660; Mature: 660

Protein sequence:

>660_residues
MYNLDLFPGRHWYMHAKMTNMLFSKMIALMAVLVMLLWVSAPVQAEAFSANFKGTDIHEFISTASKALGKTIIIDDAVKG
KVTVRSYEPLDAGQYYQFFLNVLDVYGYAVISMPNNVLKVIPAKDGKRAALNALNTPAEGDELIMRIVPLNNIAAKEVAP
LMRQFNDSIGIGNVVFLESGNALLITGRADVVNSLVELAHEMDRTEQAGVVSVALQHASATDVVSIVTTLFRDENRTKKG
ALPGMKVVADERTNTVLIAGEPSLQARIEGIIQQLDQEKASQGNTKVIRLKYAKAQGLLEVLSGVSANLQDEKKTVGNNV
SVMKNMVIKADEQTNALIINATPDVMSNLEKVIEQLDIRRAQVLVEAVIVEMQNADGLNLGIQWANRYGGGTQFGNAQAQ
IAPGFKGGMTEVFKNANGLVTGFYSGNWAGLLTAISNQSQNNILATPSIMTLDNEDAEFSVGQDVPILTGSQTTNSDNVF
NTVARKTVGIKLKVKPQINKGQSVLLQIEQEVSSVAENSSVDKDNLGATFNIRTVNNTVLVESGETVVVGGLLDKSQSDS
KSAVPFLERIPLLGALFRSTSHKESKRNLILFIRPTVIRSTEEYAQESMRKAARFRSLDAQPLPLKTHAEAQLDRVGSTE
NGTFQRIQRQIDAFYPRGEL

Sequences:

>Translated_660_residues
MYNLDLFPGRHWYMHAKMTNMLFSKMIALMAVLVMLLWVSAPVQAEAFSANFKGTDIHEFISTASKALGKTIIIDDAVKG
KVTVRSYEPLDAGQYYQFFLNVLDVYGYAVISMPNNVLKVIPAKDGKRAALNALNTPAEGDELIMRIVPLNNIAAKEVAP
LMRQFNDSIGIGNVVFLESGNALLITGRADVVNSLVELAHEMDRTEQAGVVSVALQHASATDVVSIVTTLFRDENRTKKG
ALPGMKVVADERTNTVLIAGEPSLQARIEGIIQQLDQEKASQGNTKVIRLKYAKAQGLLEVLSGVSANLQDEKKTVGNNV
SVMKNMVIKADEQTNALIINATPDVMSNLEKVIEQLDIRRAQVLVEAVIVEMQNADGLNLGIQWANRYGGGTQFGNAQAQ
IAPGFKGGMTEVFKNANGLVTGFYSGNWAGLLTAISNQSQNNILATPSIMTLDNEDAEFSVGQDVPILTGSQTTNSDNVF
NTVARKTVGIKLKVKPQINKGQSVLLQIEQEVSSVAENSSVDKDNLGATFNIRTVNNTVLVESGETVVVGGLLDKSQSDS
KSAVPFLERIPLLGALFRSTSHKESKRNLILFIRPTVIRSTEEYAQESMRKAARFRSLDAQPLPLKTHAEAQLDRVGSTE
NGTFQRIQRQIDAFYPRGEL
>Mature_660_residues
MYNLDLFPGRHWYMHAKMTNMLFSKMIALMAVLVMLLWVSAPVQAEAFSANFKGTDIHEFISTASKALGKTIIIDDAVKG
KVTVRSYEPLDAGQYYQFFLNVLDVYGYAVISMPNNVLKVIPAKDGKRAALNALNTPAEGDELIMRIVPLNNIAAKEVAP
LMRQFNDSIGIGNVVFLESGNALLITGRADVVNSLVELAHEMDRTEQAGVVSVALQHASATDVVSIVTTLFRDENRTKKG
ALPGMKVVADERTNTVLIAGEPSLQARIEGIIQQLDQEKASQGNTKVIRLKYAKAQGLLEVLSGVSANLQDEKKTVGNNV
SVMKNMVIKADEQTNALIINATPDVMSNLEKVIEQLDIRRAQVLVEAVIVEMQNADGLNLGIQWANRYGGGTQFGNAQAQ
IAPGFKGGMTEVFKNANGLVTGFYSGNWAGLLTAISNQSQNNILATPSIMTLDNEDAEFSVGQDVPILTGSQTTNSDNVF
NTVARKTVGIKLKVKPQINKGQSVLLQIEQEVSSVAENSSVDKDNLGATFNIRTVNNTVLVESGETVVVGGLLDKSQSDS
KSAVPFLERIPLLGALFRSTSHKESKRNLILFIRPTVIRSTEEYAQESMRKAARFRSLDAQPLPLKTHAEAQLDRVGSTE
NGTFQRIQRQIDAFYPRGEL

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=624, Percent_Identity=46.7948717948718, Blast_Score=529, Evalue=1e-151,
Organism=Escherichia coli, GI1789793, Length=283, Percent_Identity=25.7950530035336, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 71953; Mature: 71953

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNLDLFPGRHWYMHAKMTNMLFSKMIALMAVLVMLLWVSAPVQAEAFSANFKGTDIHEF
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEECCCCCCHHHHH
ISTASKALGKTIIIDDAVKGKVTVRSYEPLDAGQYYQFFLNVLDVYGYAVISMPNNVLKV
HHHHHHHHCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCEEEEECCCCEEEE
IPAKDGKRAALNALNTPAEGDELIMRIVPLNNIAAKEVAPLMRQFNDSIGIGNVVFLESG
EECCCCCHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECC
NALLITGRADVVNSLVELAHEMDRTEQAGVVSVALQHASATDVVSIVTTLFRDENRTKKG
CEEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHCCHHHHHHHHHHHHHCCCCCCCC
ALPGMKVVADERTNTVLIAGEPSLQARIEGIIQQLDQEKASQGNTKVIRLKYAKAQGLLE
CCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHH
VLSGVSANLQDEKKTVGNNVSVMKNMVIKADEQTNALIINATPDVMSNLEKVIEQLDIRR
HHCCCCCCCCHHHHHHCCCHHHHHHHHEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHH
AQVLVEAVIVEMQNADGLNLGIQWANRYGGGTQFGNAQAQIAPGFKGGMTEVFKNANGLV
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCHHHHHHCCCCEE
TGFYSGNWAGLLTAISNQSQNNILATPSIMTLDNEDAEFSVGQDVPILTGSQTTNSDNVF
EEEECCCHHHHHHHHCCCCCCCEEECCEEEEECCCCCCEECCCCCCEEECCCCCCCCHHH
NTVARKTVGIKLKVKPQINKGQSVLLQIEQEVSSVAENSSVDKDNLGATFNIRTVNNTVL
HHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCEEE
VESGETVVVGGLLDKSQSDSKSAVPFLERIPLLGALFRSTSHKESKRNLILFIRPTVIRS
EECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHCC
TEEYAQESMRKAARFRSLDAQPLPLKTHAEAQLDRVGSTENGTFQRIQRQIDAFYPRGEL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MYNLDLFPGRHWYMHAKMTNMLFSKMIALMAVLVMLLWVSAPVQAEAFSANFKGTDIHEF
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEECCCCCCHHHHH
ISTASKALGKTIIIDDAVKGKVTVRSYEPLDAGQYYQFFLNVLDVYGYAVISMPNNVLKV
HHHHHHHHCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCEEEEECCCCEEEE
IPAKDGKRAALNALNTPAEGDELIMRIVPLNNIAAKEVAPLMRQFNDSIGIGNVVFLESG
EECCCCCHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEECC
NALLITGRADVVNSLVELAHEMDRTEQAGVVSVALQHASATDVVSIVTTLFRDENRTKKG
CEEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHCCHHHHHHHHHHHHHCCCCCCCC
ALPGMKVVADERTNTVLIAGEPSLQARIEGIIQQLDQEKASQGNTKVIRLKYAKAQGLLE
CCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHH
VLSGVSANLQDEKKTVGNNVSVMKNMVIKADEQTNALIINATPDVMSNLEKVIEQLDIRR
HHCCCCCCCCHHHHHHCCCHHHHHHHHEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHH
AQVLVEAVIVEMQNADGLNLGIQWANRYGGGTQFGNAQAQIAPGFKGGMTEVFKNANGLV
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCHHHHHHCCCCEE
TGFYSGNWAGLLTAISNQSQNNILATPSIMTLDNEDAEFSVGQDVPILTGSQTTNSDNVF
EEEECCCHHHHHHHHCCCCCCCEEECCEEEEECCCCCCEECCCCCCEEECCCCCCCCHHH
NTVARKTVGIKLKVKPQINKGQSVLLQIEQEVSSVAENSSVDKDNLGATFNIRTVNNTVL
HHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCEEE
VESGETVVVGGLLDKSQSDSKSAVPFLERIPLLGALFRSTSHKESKRNLILFIRPTVIRS
EECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHCC
TEEYAQESMRKAARFRSLDAQPLPLKTHAEAQLDRVGSTENGTFQRIQRQIDAFYPRGEL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2677007 [H]