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The map label for this gene is pulL [H]

Identifier: 157372476

GI number: 157372476

Start: 4706357

End: 4707451

Strand: Reverse

Name: pulL [H]

Synonym: Spro_4243

Alternate gene names: 157372476

Gene position: 4707451-4706357 (Counterclockwise)

Preceding gene: 157372477

Following gene: 157372475

Centisome position: 86.39

GC content: 59.27

Gene sequence:

>1095_bases
ATGACGTGTTCAAAACCCTTGTTGGTGCTGGCATTAGGGGAGGCCGGAGCGCCAATCCTATGGTGTCAGGGAGATCCGAG
CGAATCGCAATGGCGGCATGCGGGGGCAGATACCTTGCCGGCGACGCTGCCGATAAAGGCATCGCGATGCAGAGTCGTGG
TCTGCGTGCCGGGCCAGTCGGTGACATTGCAGAGGGTGACATTCTCTGGCCCCAGCCGCGCGGCAACGCCTCTGGCCCTG
GCTTATCAGTGTGAGGATAGCCTGCTGGAGGAGGTTGAGCAGTTGTATTGGGTAATCCTGGGTCGGCAGGACGACCACTA
TGCCCTTGCCGGTTATCGTCATGTGGATATGCAGCGCTGGCTGTCGCAATTGGAACGCTGGGGCATTGTTCCCGACAGCC
TGTTGCCAGACACGCTTTGCGGACCGGTCAACGACCGTCATTTCTACCGTTGGCGGGGAAGGTTGTTGCGCAGGACGTCG
CCGTGGTCAGGCTATTCGCTGCCGTCACAGTGGCTTTTGGCTCCGTCCGACGTCGTCGACGCCGTTCCCCTGTCTCCTTG
CGATGTGTTGTGGGACCGGGTTCGGGGAGAGGCCGAAACGGCAGTTTCACTCCTGCAGGGTAAATATAAGGCGCCGCCAC
GCTGGCAACGAAACCCGTTCTGGCGCCACTGGCCCAGCATCGCTGGGGCGGCGTTAACGCTATGCGCCCTGGCGATCGGT
GGAGCGCATTATCACCATCAGGCTAAGCAGGCCAACCAGCAACGTGACGCTTTATATCAGCGGCTTTTTGTTGGCCAGCC
GATACCGGCGGAACCTGTGAGTGAGAGTGCGCGTTATATCCGAGCGCTACAAGAAGCAAAATCTCCGCGCCAATTCTTCG
AACTTGCCGCGCAGGCGCAACGGGCCCTGCAGGGGATCCCGAAACACCGGGTCATTGGCCTGACTTTCGACGCCGACAGG
TCAATGCTGGCCATCAGATTACAGGCGCCTGAATTGCAGCCCTTCACGCAAATAAATGATGAAGGTAACACCCTGGAGCT
GCAAGTTAACCCGGCCAAACAGCAGGGGACGCTTACCCTGAAGGAAACTCAATGA

Upstream 100 bases:

>100_bases
AAGATGCAGACGCCGGTTATTACCTGCGCAGCAAATTGCATTATGAAAAAGGAAGGGTGACGGTGAAAGGCCGTCAAACC
GGGCAGGGAGAGAAGGTTCA

Downstream 100 bases:

>100_bases
TGTCTCCAACAATGCAGCAGCGTGCGGGCAAAAAGAACGCGGCGCTTATCTTTTGCGGCATGCTCGTCGCGGTCAGCCTT
TTGGCATTTTATCTTCAGCA

Product: general secretion pathway L

Products: NA

Alternate protein names: Pullulanase secretion protein pulL [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MTCSKPLLVLALGEAGAPILWCQGDPSESQWRHAGADTLPATLPIKASRCRVVVCVPGQSVTLQRVTFSGPSRAATPLAL
AYQCEDSLLEEVEQLYWVILGRQDDHYALAGYRHVDMQRWLSQLERWGIVPDSLLPDTLCGPVNDRHFYRWRGRLLRRTS
PWSGYSLPSQWLLAPSDVVDAVPLSPCDVLWDRVRGEAETAVSLLQGKYKAPPRWQRNPFWRHWPSIAGAALTLCALAIG
GAHYHHQAKQANQQRDALYQRLFVGQPIPAEPVSESARYIRALQEAKSPRQFFELAAQAQRALQGIPKHRVIGLTFDADR
SMLAIRLQAPELQPFTQINDEGNTLELQVNPAKQQGTLTLKETQ

Sequences:

>Translated_364_residues
MTCSKPLLVLALGEAGAPILWCQGDPSESQWRHAGADTLPATLPIKASRCRVVVCVPGQSVTLQRVTFSGPSRAATPLAL
AYQCEDSLLEEVEQLYWVILGRQDDHYALAGYRHVDMQRWLSQLERWGIVPDSLLPDTLCGPVNDRHFYRWRGRLLRRTS
PWSGYSLPSQWLLAPSDVVDAVPLSPCDVLWDRVRGEAETAVSLLQGKYKAPPRWQRNPFWRHWPSIAGAALTLCALAIG
GAHYHHQAKQANQQRDALYQRLFVGQPIPAEPVSESARYIRALQEAKSPRQFFELAAQAQRALQGIPKHRVIGLTFDADR
SMLAIRLQAPELQPFTQINDEGNTLELQVNPAKQQGTLTLKETQ
>Mature_363_residues
TCSKPLLVLALGEAGAPILWCQGDPSESQWRHAGADTLPATLPIKASRCRVVVCVPGQSVTLQRVTFSGPSRAATPLALA
YQCEDSLLEEVEQLYWVILGRQDDHYALAGYRHVDMQRWLSQLERWGIVPDSLLPDTLCGPVNDRHFYRWRGRLLRRTSP
WSGYSLPSQWLLAPSDVVDAVPLSPCDVLWDRVRGEAETAVSLLQGKYKAPPRWQRNPFWRHWPSIAGAALTLCALAIGG
AHYHHQAKQANQQRDALYQRLFVGQPIPAEPVSESARYIRALQEAKSPRQFFELAAQAQRALQGIPKHRVIGLTFDADRS
MLAIRLQAPELQPFTQINDEGNTLELQVNPAKQQGTLTLKETQ

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG3297

COG function: function code U; Type II secretory pathway, component PulL

Gene ontology:

Cell location: Cell inner membrane (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP L family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007812 [H]

Pfam domain/function: PF05134 GspL [H]

EC number: NA

Molecular weight: Translated: 40789; Mature: 40658

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCSKPLLVLALGEAGAPILWCQGDPSESQWRHAGADTLPATLPIKASRCRVVVCVPGQS
CCCCCCEEEEEECCCCCCEEEECCCCCHHHHHHCCCCCCCCCCCEECCCEEEEEEECCCC
VTLQRVTFSGPSRAATPLALAYQCEDSLLEEVEQLYWVILGRQDDHYALAGYRHVDMQRW
EEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHEEEECCCCCCEEECCEEHHHHHHH
LSQLERWGIVPDSLLPDTLCGPVNDRHFYRWRGRLLRRTSPWSGYSLPSQWLLAPSDVVD
HHHHHHCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCCHHHHH
AVPLSPCDVLWDRVRGEAETAVSLLQGKYKAPPRWQRNPFWRHWPSIAGAALTLCALAIG
CCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GAHYHHQAKQANQQRDALYQRLFVGQPIPAEPVSESARYIRALQEAKSPRQFFELAAQAQ
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RALQGIPKHRVIGLTFDADRSMLAIRLQAPELQPFTQINDEGNTLELQVNPAKQQGTLTL
HHHHCCCCCEEEEEEECCCCCEEEEEEECCCCCCHHEECCCCCEEEEEECCCCCCCCEEE
KETQ
ECCC
>Mature Secondary Structure 
TCSKPLLVLALGEAGAPILWCQGDPSESQWRHAGADTLPATLPIKASRCRVVVCVPGQS
CCCCCEEEEEECCCCCCEEEECCCCCHHHHHHCCCCCCCCCCCEECCCEEEEEEECCCC
VTLQRVTFSGPSRAATPLALAYQCEDSLLEEVEQLYWVILGRQDDHYALAGYRHVDMQRW
EEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHEEEECCCCCCEEECCEEHHHHHHH
LSQLERWGIVPDSLLPDTLCGPVNDRHFYRWRGRLLRRTSPWSGYSLPSQWLLAPSDVVD
HHHHHHCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCCHHHHH
AVPLSPCDVLWDRVRGEAETAVSLLQGKYKAPPRWQRNPFWRHWPSIAGAALTLCALAIG
CCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GAHYHHQAKQANQQRDALYQRLFVGQPIPAEPVSESARYIRALQEAKSPRQFFELAAQAQ
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RALQGIPKHRVIGLTFDADRSMLAIRLQAPELQPFTQINDEGNTLELQVNPAKQQGTLTL
HHHHCCCCCEEEEEEECCCCCEEEEEEECCCCCCHHEECCCCCEEEEEECCCCCCCCEEE
KETQ
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2162463 [H]