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The map label for this gene is outK [H]

Identifier: 157372477

GI number: 157372477

Start: 4707448

End: 4708404

Strand: Reverse

Name: outK [H]

Synonym: Spro_4244

Alternate gene names: 157372477

Gene position: 4708404-4707448 (Counterclockwise)

Preceding gene: 157372478

Following gene: 157372476

Centisome position: 86.41

GC content: 53.29

Gene sequence:

>957_bases
ATGAAAAAATCACGGGGAATGGCGCTGCTGATCGTCCTGTTATTGCTGGCGCTGATGGCCACTTTGTCGATGGGCATTCA
TCAGTTTTGGCAGCTTACTTTCACCCGAACATTGCACGCGCAAACGACTTTTCAGGCCAAATGGGATCTGTTGGCGGGGG
AGCAATTTGTTCGGCAGTTATTGGTGGAAAGTTTGCAAGAACAGACCTCCGTTAATCTGGGGCAGCGATGGGCGACGCCT
GGGATGATACGCATGGAAGACAGGGGGATTTCTTATGTTATCAAGGATGCCCAGGCCTGTTTTAACATCAATACGTTACG
CTACCACTACTTGCCGGATAAGCAAAAATCGCCCCCGCAGGACCCGCCGCCGGCCCCAGCGTCCGAGGTTAAAATCGACA
GAGATATTGCCCGTCAGGTGTTCGGCGCTCTGTTATCGAATCTGGCGTTCACCGATGATGAAATCAAAGGCATCGGCAGT
GCGATAGAACAGCGGCTGATGCCGGAGAATTCGCTTTTTACTGACGTTTCCGAATTGCGCGCATTTCCTTTCATTGGCCG
CCAGCAGTATCTGCGTTTACTGCCCTGGTTATGTGCATTGCCGGAACGTAAGCCGGCAATCAACATCAACACGCTGGATG
AGGCTCACTTACCTCTGCTGCGCGCCTTGTTTCTGAACCAGGCCAGCGACACTCAATTGAAGAAATGGCTCAATGCCCGT
CCCGAGGCAGGTTGGCGCGCGCTGGACGATCAGGATTGGCAAAAAGCACTGGCCGGGTTGCGCCTGTCCTCGCCGGCGGG
GGAGAAAATGTTGGTGACGGAGAGCCGTTATTTTAACGTCCTGCTGCTGACTCAAGATGCAGACGCCGGTTATTACCTGC
GCAGCAAATTGCATTATGAAAAAGGAAGGGTGACGGTGAAAGGCCGTCAAACCGGGCAGGGAGAGAAGGTTCAATGA

Upstream 100 bases:

>100_bases
TTAACGGTGCCGGTTTACCTCAGGCGATAGAGGTGTCCGTGACCTTGTTCGACCGGGGGGAACTACAGCGTATTTTCTTG
ACGGCCGATGGAGAGGCGGC

Downstream 100 bases:

>100_bases
CGTGTTCAAAACCCTTGTTGGTGCTGGCATTAGGGGAGGCCGGAGCGCCAATCCTATGGTGTCAGGGAGATCCGAGCGAA
TCGCAATGGCGGCATGCGGG

Product: general secretion pathway protein K

Products: NA

Alternate protein names: Pectic enzymes secretion protein OUTK [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKKSRGMALLIVLLLLALMATLSMGIHQFWQLTFTRTLHAQTTFQAKWDLLAGEQFVRQLLVESLQEQTSVNLGQRWATP
GMIRMEDRGISYVIKDAQACFNINTLRYHYLPDKQKSPPQDPPPAPASEVKIDRDIARQVFGALLSNLAFTDDEIKGIGS
AIEQRLMPENSLFTDVSELRAFPFIGRQQYLRLLPWLCALPERKPAININTLDEAHLPLLRALFLNQASDTQLKKWLNAR
PEAGWRALDDQDWQKALAGLRLSSPAGEKMLVTESRYFNVLLLTQDADAGYYLRSKLHYEKGRVTVKGRQTGQGEKVQ

Sequences:

>Translated_318_residues
MKKSRGMALLIVLLLLALMATLSMGIHQFWQLTFTRTLHAQTTFQAKWDLLAGEQFVRQLLVESLQEQTSVNLGQRWATP
GMIRMEDRGISYVIKDAQACFNINTLRYHYLPDKQKSPPQDPPPAPASEVKIDRDIARQVFGALLSNLAFTDDEIKGIGS
AIEQRLMPENSLFTDVSELRAFPFIGRQQYLRLLPWLCALPERKPAININTLDEAHLPLLRALFLNQASDTQLKKWLNAR
PEAGWRALDDQDWQKALAGLRLSSPAGEKMLVTESRYFNVLLLTQDADAGYYLRSKLHYEKGRVTVKGRQTGQGEKVQ
>Mature_318_residues
MKKSRGMALLIVLLLLALMATLSMGIHQFWQLTFTRTLHAQTTFQAKWDLLAGEQFVRQLLVESLQEQTSVNLGQRWATP
GMIRMEDRGISYVIKDAQACFNINTLRYHYLPDKQKSPPQDPPPAPASEVKIDRDIARQVFGALLSNLAFTDDEIKGIGS
AIEQRLMPENSLFTDVSELRAFPFIGRQQYLRLLPWLCALPERKPAININTLDEAHLPLLRALFLNQASDTQLKKWLNAR
PEAGWRALDDQDWQKALAGLRLSSPAGEKMLVTESRYFNVLLLTQDADAGYYLRSKLHYEKGRVTVKGRQTGQGEKVQ

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG3156

COG function: function code U; Type II secretory pathway, component PulK

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP K family [H]

Homologues:

Organism=Escherichia coli, GI1789729, Length=272, Percent_Identity=28.3088235294118, Blast_Score=98, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005628 [H]

Pfam domain/function: PF03934 GspK [H]

EC number: NA

Molecular weight: Translated: 36134; Mature: 36134

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSRGMALLIVLLLLALMATLSMGIHQFWQLTFTRTLHAQTTFQAKWDLLAGEQFVRQL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEHHHHHHHHHHHHHHHHH
LVESLQEQTSVNLGQRWATPGMIRMEDRGISYVIKDAQACFNINTLRYHYLPDKQKSPPQ
HHHHHHHHHCCCCCHHCCCCCEEEECCCCCEEEEHHHHHHCCCCCEEEEECCCCCCCCCC
DPPPAPASEVKIDRDIARQVFGALLSNLAFTDDEIKGIGSAIEQRLMPENSLFTDVSELR
CCCCCCHHHEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AFPFIGRQQYLRLLPWLCALPERKPAININTLDEAHLPLLRALFLNQASDTQLKKWLNAR
HCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCC
PEAGWRALDDQDWQKALAGLRLSSPAGEKMLVTESRYFNVLLLTQDADAGYYLRSKLHYE
CCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEEEECCCCCHHHHHHHHHC
KGRVTVKGRQTGQGEKVQ
CCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKKSRGMALLIVLLLLALMATLSMGIHQFWQLTFTRTLHAQTTFQAKWDLLAGEQFVRQL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEHHHHHHHHHHHHHHHHH
LVESLQEQTSVNLGQRWATPGMIRMEDRGISYVIKDAQACFNINTLRYHYLPDKQKSPPQ
HHHHHHHHHCCCCCHHCCCCCEEEECCCCCEEEEHHHHHHCCCCCEEEEECCCCCCCCCC
DPPPAPASEVKIDRDIARQVFGALLSNLAFTDDEIKGIGSAIEQRLMPENSLFTDVSELR
CCCCCCHHHEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AFPFIGRQQYLRLLPWLCALPERKPAININTLDEAHLPLLRALFLNQASDTQLKKWLNAR
HCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCC
PEAGWRALDDQDWQKALAGLRLSSPAGEKMLVTESRYFNVLLLTQDADAGYYLRSKLHYE
CCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEEEECCCCCHHHHHHHHHC
KGRVTVKGRQTGQGEKVQ
CCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1429461; 1992458 [H]