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The map label for this gene is tapD [H]

Identifier: 157372474

GI number: 157372474

Start: 4705029

End: 4705892

Strand: Reverse

Name: tapD [H]

Synonym: Spro_4241

Alternate gene names: 157372474

Gene position: 4705892-4705029 (Counterclockwise)

Preceding gene: 157372475

Following gene: 157372472

Centisome position: 86.36

GC content: 56.71

Gene sequence:

>864_bases
ATGGCGTTTTACACGGATACCTCTCTTTGGCTGGCGTTCTCGGGGCTGGTGGGGTTGTGCGTCGGCAGCTTGCTTAATGT
GGTGATCTATCGCTTGCCGCTGATGATGCAGCAGGAAGAAACAACCTTCGTGATGGATTATTTGACCCGACACCCCAGCG
CCGTACCAACGCCCGCCGGAAAGAGCATTTTCCCCGATAAAAACCGCGCAGTCCTGAACCTGTTTCTGCCGCGTTCACAT
TGCCCCAACTGCAAACATACCGTCGCCTGTTACGATAATATTCCGCTGCTCGGTTGGTTGCTTTTGGCCGGGCGCTGTCG
CTATTGCCGGCAGGCTATCGGCTGGGCTTACCCGGCGGTTGAGCTGGCGATGGCGGTTATCGCCACCCTGTTGGCCTGCT
ATCTGCCCCCGGGAGCGGACTGGGTGATGATGATGGTTTCTAGCGCCTTGCTGCTCTCGCTGGCCATCATCGATTTTCGC
CATCAAATATTGCCGGATATCCTGACTTTGACGTTGTTGTGGTGCGGCCTGTTTTGGCACTGGCTGGAGCAGCGTGATTT
TTTGGGTGATGCGGTGCTCGGCGCTATCGCCGGTTACCTGGTATTGTGGTTGCTCTATTGGAGTCTGCGGCTGATGACCG
GGAAGGAAGGGCTGGGGTATGGCGATTTCAAGCTGCTGGCGGCGCTCGGTGCCTGGCTTGGTTGGCAGAAGTTGCCGGAG
ATCCTGCTGATAGCCTCGACGGCAACGCTGTTTGTTATCCTGTTGTTCGCGAAAAAGCGAGCAAATAGCTGGAGCCAGCC
CCAACCTTTCGGTCCGGGGCTGTGTGTTGCAGGGGCGATTTGCCTGATCTTACAGCTGAAATAG

Upstream 100 bases:

>100_bases
GGGGGTCAATTCGATTGATCTCAGCCTGCATGGGGATGAGGTCAGGGTGCAGGCATTGACTCTTCAAGTCATCTCAGTTC
ATTGACCAATAGGAATGACC

Downstream 100 bases:

>100_bases
CGTTATTGTCGGCGAGAGGCTTCAATAAAAGCGGCTAACGATGAGTTAAAATAGGCACACTTCTGGGCAACCGGCGTGGG
GTCCTGGTTCAGCTTGTTAA

Product: prepilin peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MAFYTDTSLWLAFSGLVGLCVGSLLNVVIYRLPLMMQQEETTFVMDYLTRHPSAVPTPAGKSIFPDKNRAVLNLFLPRSH
CPNCKHTVACYDNIPLLGWLLLAGRCRYCRQAIGWAYPAVELAMAVIATLLACYLPPGADWVMMMVSSALLLSLAIIDFR
HQILPDILTLTLLWCGLFWHWLEQRDFLGDAVLGAIAGYLVLWLLYWSLRLMTGKEGLGYGDFKLLAALGAWLGWQKLPE
ILLIASTATLFVILLFAKKRANSWSQPQPFGPGLCVAGAICLILQLK

Sequences:

>Translated_287_residues
MAFYTDTSLWLAFSGLVGLCVGSLLNVVIYRLPLMMQQEETTFVMDYLTRHPSAVPTPAGKSIFPDKNRAVLNLFLPRSH
CPNCKHTVACYDNIPLLGWLLLAGRCRYCRQAIGWAYPAVELAMAVIATLLACYLPPGADWVMMMVSSALLLSLAIIDFR
HQILPDILTLTLLWCGLFWHWLEQRDFLGDAVLGAIAGYLVLWLLYWSLRLMTGKEGLGYGDFKLLAALGAWLGWQKLPE
ILLIASTATLFVILLFAKKRANSWSQPQPFGPGLCVAGAICLILQLK
>Mature_286_residues
AFYTDTSLWLAFSGLVGLCVGSLLNVVIYRLPLMMQQEETTFVMDYLTRHPSAVPTPAGKSIFPDKNRAVLNLFLPRSHC
PNCKHTVACYDNIPLLGWLLLAGRCRYCRQAIGWAYPAVELAMAVIATLLACYLPPGADWVMMMVSSALLLSLAIIDFRH
QILPDILTLTLLWCGLFWHWLEQRDFLGDAVLGAIAGYLVLWLLYWSLRLMTGKEGLGYGDFKLLAALGAWLGWQKLPEI
LLIASTATLFVILLFAKKRANSWSQPQPFGPGLCVAGAICLILQLK

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the tapA pilin precursor during membrane translocation [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=269, Percent_Identity=38.6617100371747, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1789732, Length=97, Percent_Identity=62.8865979381443, Blast_Score=94, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 31951; Mature: 31819

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFYTDTSLWLAFSGLVGLCVGSLLNVVIYRLPLMMQQEETTFVMDYLTRHPSAVPTPAG
CCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCC
KSIFPDKNRAVLNLFLPRSHCPNCKHTVACYDNIPLLGWLLLAGRCRYCRQAIGWAYPAV
CCCCCCCCCEEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
ELAMAVIATLLACYLPPGADWVMMMVSSALLLSLAIIDFRHQILPDILTLTLLWCGLFWH
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLEQRDFLGDAVLGAIAGYLVLWLLYWSLRLMTGKEGLGYGDFKLLAALGAWLGWQKLPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ILLIASTATLFVILLFAKKRANSWSQPQPFGPGLCVAGAICLILQLK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AFYTDTSLWLAFSGLVGLCVGSLLNVVIYRLPLMMQQEETTFVMDYLTRHPSAVPTPAG
CEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCC
KSIFPDKNRAVLNLFLPRSHCPNCKHTVACYDNIPLLGWLLLAGRCRYCRQAIGWAYPAV
CCCCCCCCCEEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
ELAMAVIATLLACYLPPGADWVMMMVSSALLLSLAIIDFRHQILPDILTLTLLWCGLFWH
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLEQRDFLGDAVLGAIAGYLVLWLLYWSLRLMTGKEGLGYGDFKLLAALGAWLGWQKLPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ILLIASTATLFVILLFAKKRANSWSQPQPFGPGLCVAGAICLILQLK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA