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The map label for this gene is yiaJ [H]

Identifier: 157372162

GI number: 157372162

Start: 4351526

End: 4352344

Strand: Reverse

Name: yiaJ [H]

Synonym: Spro_3928

Alternate gene names: 157372162

Gene position: 4352344-4351526 (Counterclockwise)

Preceding gene: 157372176

Following gene: 157372161

Centisome position: 79.88

GC content: 54.46

Gene sequence:

>819_bases
ATGAGCCTGAAAGCTGTCGACGAGAAAGATGACAAGAAAGACAAACCCCTGGGCAGCCAAAGCCTGTTCCGTGGTTTGCA
ACTGATTGAGATCCTGAGCAACTTCCCTAACGGCTGCCCACTGGCGCACCTGTCTGAGTTGGCCGGCATGAATAAAAGTA
CCGTCCATCGCCTGCTGCAAGGGCTGCATACCAGCGGCTATGTCACTCAGGCGCCGTCGCCCGGCAGCTATCGCCTGACC
ACCAAGTTTATTTCTGTCGGTCAGAAGGCGCTTTCTTCGCTGAATATTATCCATGTCGCTGCGCCGCATCTGGAACAGCT
CAATCTGGAAGTGGGGGAGACGGTCAACTTCTCCAGCCGCGAAGACGACCATGTCATTTTGATCTACAAGCTGGAGCCAA
CCACCGGCATGATGCGCACTCGTGCCTATATAGGCCAGCATATGCCGCTGTATTGCTCGGCGATGGGCAAGATTTTTATG
GCCTACGGCAACAGCGATTATCCCGATCGTTACTGGCAAACCCATCAGCATGAAATTCAGACGTTGACGCGCAATACCAT
CACCGAGTTGCCGAAGATGTATGAGGAACTGGCCGATATTCGCAAGCAGGGCATGGCGATGGATCGCGAAGAGAATGAGC
TGGGGGTGTCCTGCGTGGCGGCACCGGTATTTGATATTCAACAGCGGGTGCCTTATGCGGTGTCGGTCTCTTTGCCGACC
GCCAAACTGCAACAGATTGGTGTCAAAGCCCTGATTAAGCCGGTGCTGGCGACCGCACAGCGTATTTCTCAGGAGCTGGG
ATTTGTTATTCCCGCCTGA

Upstream 100 bases:

>100_bases
GTCTTCACCTGATGCGGGCCTTGGGGTAACATTCCCGGCAAAGGATCCGTTTGCCACGCCGTGGCGTTGGCTGGCGGCCC
GACACTGGAGAAATTGCGCT

Downstream 100 bases:

>100_bases
TTATTAGCATTGCTGATAGTTGATTAGAAATATCAATTTCACCTAATAATTTTTTCCTCCTATGATGCGCGTATTGACCG
ATACGCGCCGCTGAACGGCG

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: YiaKLMNOPQRS operon repressor; YiaK-S operon repressor [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MSLKAVDEKDDKKDKPLGSQSLFRGLQLIEILSNFPNGCPLAHLSELAGMNKSTVHRLLQGLHTSGYVTQAPSPGSYRLT
TKFISVGQKALSSLNIIHVAAPHLEQLNLEVGETVNFSSREDDHVILIYKLEPTTGMMRTRAYIGQHMPLYCSAMGKIFM
AYGNSDYPDRYWQTHQHEIQTLTRNTITELPKMYEELADIRKQGMAMDREENELGVSCVAAPVFDIQQRVPYAVSVSLPT
AKLQQIGVKALIKPVLATAQRISQELGFVIPA

Sequences:

>Translated_272_residues
MSLKAVDEKDDKKDKPLGSQSLFRGLQLIEILSNFPNGCPLAHLSELAGMNKSTVHRLLQGLHTSGYVTQAPSPGSYRLT
TKFISVGQKALSSLNIIHVAAPHLEQLNLEVGETVNFSSREDDHVILIYKLEPTTGMMRTRAYIGQHMPLYCSAMGKIFM
AYGNSDYPDRYWQTHQHEIQTLTRNTITELPKMYEELADIRKQGMAMDREENELGVSCVAAPVFDIQQRVPYAVSVSLPT
AKLQQIGVKALIKPVLATAQRISQELGFVIPA
>Mature_271_residues
SLKAVDEKDDKKDKPLGSQSLFRGLQLIEILSNFPNGCPLAHLSELAGMNKSTVHRLLQGLHTSGYVTQAPSPGSYRLTT
KFISVGQKALSSLNIIHVAAPHLEQLNLEVGETVNFSSREDDHVILIYKLEPTTGMMRTRAYIGQHMPLYCSAMGKIFMA
YGNSDYPDRYWQTHQHEIQTLTRNTITELPKMYEELADIRKQGMAMDREENELGVSCVAAPVFDIQQRVPYAVSVSLPTA
KLQQIGVKALIKPVLATAQRISQELGFVIPA

Specific function: Negatively controls the transcription of the yiaKLMNOPQRS operon, which may be involved in the utilization of 2,3-diketo-L-gulonate [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1789999, Length=267, Percent_Identity=75.6554307116105, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1788131, Length=228, Percent_Identity=30.2631578947368, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1786468, Length=251, Percent_Identity=30.2788844621514, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI87082359, Length=250, Percent_Identity=32, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786715, Length=255, Percent_Identity=28.2352941176471, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1790752, Length=220, Percent_Identity=28.6363636363636, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788581, Length=259, Percent_Identity=27.4131274131274, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI145693096, Length=143, Percent_Identity=31.4685314685315, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 30267; Mature: 30136

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKAVDEKDDKKDKPLGSQSLFRGLQLIEILSNFPNGCPLAHLSELAGMNKSTVHRLLQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHH
GLHTSGYVTQAPSPGSYRLTTKFISVGQKALSSLNIIHVAAPHLEQLNLEVGETVNFSSR
HHCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHCCEEEEECCCHHHCCCCCCCEECCCCC
EDDHVILIYKLEPTTGMMRTRAYIGQHMPLYCSAMGKIFMAYGNSDYPDRYWQTHQHEIQ
CCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHEEEECCCCCCCHHHHHHHHHHHH
TLTRNTITELPKMYEELADIRKQGMAMDREENELGVSCVAAPVFDIQQRVPYAVSVSLPT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCH
AKLQQIGVKALIKPVLATAQRISQELGFVIPA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SLKAVDEKDDKKDKPLGSQSLFRGLQLIEILSNFPNGCPLAHLSELAGMNKSTVHRLLQ
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHH
GLHTSGYVTQAPSPGSYRLTTKFISVGQKALSSLNIIHVAAPHLEQLNLEVGETVNFSSR
HHCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHCCEEEEECCCHHHCCCCCCCEECCCCC
EDDHVILIYKLEPTTGMMRTRAYIGQHMPLYCSAMGKIFMAYGNSDYPDRYWQTHQHEIQ
CCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHEEEECCCCCCCHHHHHHHHHHHH
TLTRNTITELPKMYEELADIRKQGMAMDREENELGVSCVAAPVFDIQQRVPYAVSVSLPT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCH
AKLQQIGVKALIKPVLATAQRISQELGFVIPA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]