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The map label for this gene is shiA [H]

Identifier: 157372136

GI number: 157372136

Start: 4321567

End: 4322892

Strand: Reverse

Name: shiA [H]

Synonym: Spro_3902

Alternate gene names: 157372136

Gene position: 4322892-4321567 (Counterclockwise)

Preceding gene: 157372137

Following gene: 157372134

Centisome position: 79.34

GC content: 59.35

Gene sequence:

>1326_bases
ATGAGCACTTCAACCACACAGCCGTCAATCGCGGAAGCCTCCACTGCCGAGGCTACCGCCCACCCGCCAAACACCGGCAA
GCTGGCGGCGGCCAGTTCGATTGGCACCGCGCTGGAATGGTATGACTTCACCGTCTACAACATCATGGCGGCGCTGATTT
TCAACCACGTATTTTTCCCCTCTTTCGATCCGCTGGTGGGCACCATTTTGGCGTTTTCCACTTACGCGGTGGGTTATGTT
TCGCGGCCGATTGGCGGTATCGTCTTTGGTCATCTGGGTGACGTGCTGGGACGGCGTTTTGTATTGGTGGCCACGCTGGT
GATCATGGGGGTGACAACCGCGCTGATGGGGTTGCTACCCGGTTATGCCAGCTGGGGGATTTGGAGCCCGCTGCTGTTGG
TGACGCTGCGCTTTATTCAGGGAATTGCGCTGGGGGGCGAATGGGCGGGGGCGGTATTGCTGTCGATGGAGCACGGCAAG
CCTCATCAGCGAGGACGCAACGCCTCCTTTGCTCAGGTTGGCCCTTCCTGCGGAACGCTGATCGGCACCGGGCTTATCAC
GCTGGTGACGGTGGCGATGACGGCGGAGGATTTCCAGCAGTGGGGCTGGCGTATTCCCTTCCTGCTTAGCCTGGTACTGG
TGGTGTTTGGCCTGTGGCTACGCCGTGGCGTGGGGGAAACGCCGGCGTTTCTGAAACTGGAGGCATCGAAGGACGTTACC
CATTCACCAATCCGCGAAGTTTTCACCCAGCATCGGCGTCCGCTGTTGATCGCCGGAGGTTCGCGTATTGGTTCCGACGT
GCTGTATGCGCTGGTGGTGGTTTTTACCCTGACCTACGTCACCACGGTACTGAACCTGCCGCGCCCGGTGGCGTTGGCTG
CCACCATGTTGGGGGCGATCGGTAACGCGATTACCGTGCCGATGTTCGGCGCACTGTCCGATCGTTTTGGCCGTCGGCCG
GTGTATATCGGTGGGGCTTTGGCGGCGATGGTTTGGGCATTTGTGTTCTTTATGCTGCTCGACAGCAGCCAGCCGGTGCT
GATCTGCCTGGCGGTGGTGGTCGGTCTGTTGATCCATGCGGCAATGTATGGCCCGCAGGCGGCCTTTATCACCGAACAAT
TCCCAACCCGGGTACGTTATGCGGGGTCGTCGTTGGCTTATACGTTGGCAGGTATTATCGGCGGTGGCTTTGCGCCGCTG
ATCATCGCCTCATTGTTCAAGTCTTACGACAGCACGCTGGCTATTTCGGCCTATGTGGTGCTGGCACTGCTAATCACACT
GTGGGCAGTGATTGCCGCGAAGGAAACGGCACACAAGCCGTTATGA

Upstream 100 bases:

>100_bases
AGCGGCTGAGTGAGTTGGCCTGTAGCCAACTGAAAATCCCCGCCACCGATGCCCTGCATTATGCCCTTGGCTGGCAATTG
ACCACACAGGAGCTTTCATC

Downstream 100 bases:

>100_bases
AAGGCGTGCCGGGTGAAGGGATCACCCGGCCTGTCACAATAGCAACGCTGTTACTTCAGCCAGCCTTTGCGTTGTGCGTC
CTGCAGGTGTTGCAGCAGGT

Product: major facilitator transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSTSTTQPSIAEASTAEATAHPPNTGKLAAASSIGTALEWYDFTVYNIMAALIFNHVFFPSFDPLVGTILAFSTYAVGYV
SRPIGGIVFGHLGDVLGRRFVLVATLVIMGVTTALMGLLPGYASWGIWSPLLLVTLRFIQGIALGGEWAGAVLLSMEHGK
PHQRGRNASFAQVGPSCGTLIGTGLITLVTVAMTAEDFQQWGWRIPFLLSLVLVVFGLWLRRGVGETPAFLKLEASKDVT
HSPIREVFTQHRRPLLIAGGSRIGSDVLYALVVVFTLTYVTTVLNLPRPVALAATMLGAIGNAITVPMFGALSDRFGRRP
VYIGGALAAMVWAFVFFMLLDSSQPVLICLAVVVGLLIHAAMYGPQAAFITEQFPTRVRYAGSSLAYTLAGIIGGGFAPL
IIASLFKSYDSTLAISAYVVLALLITLWAVIAAKETAHKPL

Sequences:

>Translated_441_residues
MSTSTTQPSIAEASTAEATAHPPNTGKLAAASSIGTALEWYDFTVYNIMAALIFNHVFFPSFDPLVGTILAFSTYAVGYV
SRPIGGIVFGHLGDVLGRRFVLVATLVIMGVTTALMGLLPGYASWGIWSPLLLVTLRFIQGIALGGEWAGAVLLSMEHGK
PHQRGRNASFAQVGPSCGTLIGTGLITLVTVAMTAEDFQQWGWRIPFLLSLVLVVFGLWLRRGVGETPAFLKLEASKDVT
HSPIREVFTQHRRPLLIAGGSRIGSDVLYALVVVFTLTYVTTVLNLPRPVALAATMLGAIGNAITVPMFGALSDRFGRRP
VYIGGALAAMVWAFVFFMLLDSSQPVLICLAVVVGLLIHAAMYGPQAAFITEQFPTRVRYAGSSLAYTLAGIIGGGFAPL
IIASLFKSYDSTLAISAYVVLALLITLWAVIAAKETAHKPL
>Mature_440_residues
STSTTQPSIAEASTAEATAHPPNTGKLAAASSIGTALEWYDFTVYNIMAALIFNHVFFPSFDPLVGTILAFSTYAVGYVS
RPIGGIVFGHLGDVLGRRFVLVATLVIMGVTTALMGLLPGYASWGIWSPLLLVTLRFIQGIALGGEWAGAVLLSMEHGKP
HQRGRNASFAQVGPSCGTLIGTGLITLVTVAMTAEDFQQWGWRIPFLLSLVLVVFGLWLRRGVGETPAFLKLEASKDVTH
SPIREVFTQHRRPLLIAGGSRIGSDVLYALVVVFTLTYVTTVLNLPRPVALAATMLGAIGNAITVPMFGALSDRFGRRPV
YIGGALAAMVWAFVFFMLLDSSQPVLICLAVVVGLLIHAAMYGPQAAFITEQFPTRVRYAGSSLAYTLAGIIGGGFAPLI
IASLFKSYDSTLAISAYVVLALLITLWAVIAAKETAHKPL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=426, Percent_Identity=37.793427230047, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1789941, Length=431, Percent_Identity=39.2111368909513, Blast_Score=262, Evalue=2e-71,
Organism=Escherichia coli, GI1790550, Length=430, Percent_Identity=33.7209302325581, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1788942, Length=433, Percent_Identity=31.4087759815243, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI87082404, Length=424, Percent_Identity=25.2358490566038, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082231, Length=459, Percent_Identity=22.8758169934641, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46976; Mature: 46845

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSTTQPSIAEASTAEATAHPPNTGKLAAASSIGTALEWYDFTVYNIMAALIFNHVFFP
CCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SFDPLVGTILAFSTYAVGYVSRPIGGIVFGHLGDVLGRRFVLVATLVIMGVTTALMGLLP
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYASWGIWSPLLLVTLRFIQGIALGGEWAGAVLLSMEHGKPHQRGRNASFAQVGPSCGTL
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEECCCCCHHCCCCCCHHHCCCCHHHH
IGTGLITLVTVAMTAEDFQQWGWRIPFLLSLVLVVFGLWLRRGVGETPAFLKLEASKDVT
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCC
HSPIREVFTQHRRPLLIAGGSRIGSDVLYALVVVFTLTYVTTVLNLPRPVALAATMLGAI
HHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
GNAITVPMFGALSDRFGRRPVYIGGALAAMVWAFVFFMLLDSSQPVLICLAVVVGLLIHA
CCHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AMYGPQAAFITEQFPTRVRYAGSSLAYTLAGIIGGGFAPLIIASLFKSYDSTLAISAYVV
HHCCCCHHHHHHHCCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHH
LALLITLWAVIAAKETAHKPL
HHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STSTTQPSIAEASTAEATAHPPNTGKLAAASSIGTALEWYDFTVYNIMAALIFNHVFFP
CCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SFDPLVGTILAFSTYAVGYVSRPIGGIVFGHLGDVLGRRFVLVATLVIMGVTTALMGLLP
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYASWGIWSPLLLVTLRFIQGIALGGEWAGAVLLSMEHGKPHQRGRNASFAQVGPSCGTL
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEECCCCCHHCCCCCCHHHCCCCHHHH
IGTGLITLVTVAMTAEDFQQWGWRIPFLLSLVLVVFGLWLRRGVGETPAFLKLEASKDVT
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCC
HSPIREVFTQHRRPLLIAGGSRIGSDVLYALVVVFTLTYVTTVLNLPRPVALAATMLGAI
HHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
GNAITVPMFGALSDRFGRRPVYIGGALAAMVWAFVFFMLLDSSQPVLICLAVVVGLLIHA
CCHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AMYGPQAAFITEQFPTRVRYAGSSLAYTLAGIIGGGFAPLIIASLFKSYDSTLAISAYVV
HHCCCCHHHHHHHCCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHH
LALLITLWAVIAAKETAHKPL
HHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]