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The map label for this gene is intS [H]

Identifier: 157372122

GI number: 157372122

Start: 4305958

End: 4307172

Strand: Reverse

Name: intS [H]

Synonym: Spro_3887

Alternate gene names: 157372122

Gene position: 4307172-4305958 (Counterclockwise)

Preceding gene: 157372123

Following gene: 157372121

Centisome position: 79.05

GC content: 52.26

Gene sequence:

>1215_bases
ATGCCCCTCACAGACCTTGAGCTACGGCGCTCTAAACCGCAAGACAAGCCCTATACACTCAGTGATGGCGGTGGGCTTTC
TATACTCATTGAGCCGAACGGTTCCAAAGGCTGGCGCTTTCGCTACCGCTTCGACGGTAAACCAAAAATGCTTTCCCTCG
GCACATACCCCACAGTTTCACTAACCGACGCAAGGCAAAAGCGCGATGAGGCTAAAAAGCTGGTGGTGTCAGGTATCAAT
CCCAGCGAAGCCAGGAAGCAGCAGAAACGAGATCGGCAAGATGTTACTGGCAATACCTTTGAGGGGATAGCGCGGGAATG
GTACGAAAAGCGGCGCGATCGCTGGTCAGAGTCATACGCAGAAGAGATGATGAAAACGTTTGAGAGCGATGTTTTCCCAT
ACATCGGCAATCGGCCAATTGCCGAGATAAAGCCAATGGAGCTTATGGGGACGCTTTCTAAACTGGATGAACGCGGCGCA
ACAGAGAAATTGCGCAAGGTGCGCCAGCGATGCGGGGAGGTATGGAAATACGCCATCGTTACTGGCCGGGCTGAATATAA
CCCAGCCCCGGATCTCGCCAGTGCGTTCTCACCGCATAAAAAAGAGCACTATGCGTTCTTGTCAGTTGCCGAGCTGCCCG
AGTTTTTCCGCATCTTTAACACATATACCGGCAGCCTGATCGTTAAGCTGGCTATGCGCTTGCTTATTATCACAGGGACA
AGGCCGGGAGAGCTGCGCAAGGCTGAATGGTCGGAGTTTGATTTTGATAACCGGCTTTGGGAAGTACCGCCTGAACGCAT
GAAGAAACGTCGCCCTCATTGCGTGCCACTATCTGATCAGGCTATCAGCATTCTGGAACAGCTAAAACCGCTGACCGGTC
ATTACAGCTTTGTGTTTCCGGGCAGGATCCACCACAGCAAGCCAATAAGCGAAATGGCTATGAATGTGCTGGTTCGCCGT
ATTGGTTATGCCGGGCGTGTAACGGGGCACGGATTCCGGCATACCATGAGTACCATCCTGCATGAACAAGGCTATAACAC
TGCGTGGATTGAAACCCAGTTGGCCCACGTTGATAAGAATAGTATTCGTGGAACCTATAACCATGCCCAATACCTTGATG
GACGCCGGGAGATGCTTCAGTGGTATGCCGACTATATGGACAGCCTGGAGCTGGGAGGCAATGTGGTTCACGGCTCGTTC
GGAAAGCAGGCATAA

Upstream 100 bases:

>100_bases
AGCTCGTTATCTCAACGTCTACACACATCTACCCAAATCTATACATCCATGTGTATAGTAATGAGTATAGGACGTTTGAT
TTACCTCGGAGCTATACACA

Downstream 100 bases:

>100_bases
CTGGATGGATGGACAGTGCAATTAGACCTCAGTATACTTGTATAGACGAACAAAAAATGGGCTATGCCTAGGTTGATCCC
CGAAAATCCGTACATATCTG

Product: integrase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MPLTDLELRRSKPQDKPYTLSDGGGLSILIEPNGSKGWRFRYRFDGKPKMLSLGTYPTVSLTDARQKRDEAKKLVVSGIN
PSEARKQQKRDRQDVTGNTFEGIAREWYEKRRDRWSESYAEEMMKTFESDVFPYIGNRPIAEIKPMELMGTLSKLDERGA
TEKLRKVRQRCGEVWKYAIVTGRAEYNPAPDLASAFSPHKKEHYAFLSVAELPEFFRIFNTYTGSLIVKLAMRLLIITGT
RPGELRKAEWSEFDFDNRLWEVPPERMKKRRPHCVPLSDQAISILEQLKPLTGHYSFVFPGRIHHSKPISEMAMNVLVRR
IGYAGRVTGHGFRHTMSTILHEQGYNTAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLELGGNVVHGSF
GKQA

Sequences:

>Translated_404_residues
MPLTDLELRRSKPQDKPYTLSDGGGLSILIEPNGSKGWRFRYRFDGKPKMLSLGTYPTVSLTDARQKRDEAKKLVVSGIN
PSEARKQQKRDRQDVTGNTFEGIAREWYEKRRDRWSESYAEEMMKTFESDVFPYIGNRPIAEIKPMELMGTLSKLDERGA
TEKLRKVRQRCGEVWKYAIVTGRAEYNPAPDLASAFSPHKKEHYAFLSVAELPEFFRIFNTYTGSLIVKLAMRLLIITGT
RPGELRKAEWSEFDFDNRLWEVPPERMKKRRPHCVPLSDQAISILEQLKPLTGHYSFVFPGRIHHSKPISEMAMNVLVRR
IGYAGRVTGHGFRHTMSTILHEQGYNTAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLELGGNVVHGSF
GKQA
>Mature_403_residues
PLTDLELRRSKPQDKPYTLSDGGGLSILIEPNGSKGWRFRYRFDGKPKMLSLGTYPTVSLTDARQKRDEAKKLVVSGINP
SEARKQQKRDRQDVTGNTFEGIAREWYEKRRDRWSESYAEEMMKTFESDVFPYIGNRPIAEIKPMELMGTLSKLDERGAT
EKLRKVRQRCGEVWKYAIVTGRAEYNPAPDLASAFSPHKKEHYAFLSVAELPEFFRIFNTYTGSLIVKLAMRLLIITGTR
PGELRKAEWSEFDFDNRLWEVPPERMKKRRPHCVPLSDQAISILEQLKPLTGHYSFVFPGRIHHSKPISEMAMNVLVRRI
GYAGRVTGHGFRHTMSTILHEQGYNTAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLELGGNVVHGSFG
KQA

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=386, Percent_Identity=44.0414507772021, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI145693166, Length=397, Percent_Identity=42.3173803526448, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI1788974, Length=397, Percent_Identity=39.0428211586902, Blast_Score=311, Evalue=7e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 46596; Mature: 46465

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTDLELRRSKPQDKPYTLSDGGGLSILIEPNGSKGWRFRYRFDGKPKMLSLGTYPTVS
CCCCCCHHHHCCCCCCCEEECCCCCEEEEEECCCCCCEEEEEEECCCCCEEEECCCCCEE
LTDARQKRDEAKKLVVSGINPSEARKQQKRDRQDVTGNTFEGIAREWYEKRRDRWSESYA
ECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
EEMMKTFESDVFPYIGNRPIAEIKPMELMGTLSKLDERGATEKLRKVRQRCGEVWKYAIV
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHE
TGRAEYNPAPDLASAFSPHKKEHYAFLSVAELPEFFRIFNTYTGSLIVKLAMRLLIITGT
ECCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
RPGELRKAEWSEFDFDNRLWEVPPERMKKRRPHCVPLSDQAISILEQLKPLTGHYSFVFP
CCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEC
GRIHHSKPISEMAMNVLVRRIGYAGRVTGHGFRHTMSTILHEQGYNTAWIETQLAHVDKN
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHCCHH
SIRGTYNHAQYLDGRREMLQWYADYMDSLELGGNVVHGSFGKQA
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCC
>Mature Secondary Structure 
PLTDLELRRSKPQDKPYTLSDGGGLSILIEPNGSKGWRFRYRFDGKPKMLSLGTYPTVS
CCCCCHHHHCCCCCCCEEECCCCCEEEEEECCCCCCEEEEEEECCCCCEEEECCCCCEE
LTDARQKRDEAKKLVVSGINPSEARKQQKRDRQDVTGNTFEGIAREWYEKRRDRWSESYA
ECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
EEMMKTFESDVFPYIGNRPIAEIKPMELMGTLSKLDERGATEKLRKVRQRCGEVWKYAIV
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHE
TGRAEYNPAPDLASAFSPHKKEHYAFLSVAELPEFFRIFNTYTGSLIVKLAMRLLIITGT
ECCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
RPGELRKAEWSEFDFDNRLWEVPPERMKKRRPHCVPLSDQAISILEQLKPLTGHYSFVFP
CCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEC
GRIHHSKPISEMAMNVLVRRIGYAGRVTGHGFRHTMSTILHEQGYNTAWIETQLAHVDKN
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHCCHH
SIRGTYNHAQYLDGRREMLQWYADYMDSLELGGNVVHGSFGKQA
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]