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The map label for this gene is agaV [H]

Identifier: 157372082

GI number: 157372082

Start: 4257414

End: 4257890

Strand: Reverse

Name: agaV [H]

Synonym: Spro_3847

Alternate gene names: 157372082

Gene position: 4257890-4257414 (Counterclockwise)

Preceding gene: 157372083

Following gene: 157372081

Centisome position: 78.14

GC content: 59.33

Gene sequence:

>477_bases
ATGCCGAATATCCTGATGACCCGCATTGATAACCGACTGGTACATGGCCAGGTCGGTGTTACCTGGACCAACACCCTGGG
GGCCAATCTGGTGGTGGTGGCCAACGATGAAGCGGCAGCCGATCCGGTGCAGCAGAACCTGATGGATATGGTGGTGGCGG
AGGGGGTGCAGACGCGCTACTTCACGCTGCAGAAAACCATCGACGTGATCCACAAGGCGGCGGATCGCCAGAAGATCTTT
TTAGTGTGCAAAACGCCGCAGGATGTTTTGCGGCTGGTACAGGGCGGAGTACCGATCCGTTTCGTCAACGTCGGCAATAT
GCATTTCGCCGAGGGCAAGCGGCAGGTGCATAAAACGGTCTCGCTGGATGAGGGTGACGTGGCCGCCTTTCGCGAACTGG
CGGCGCTGGGCGTCGAGTGTGAGGTGCGTCGGGTGCCGGATGAAGCGGGTGAGCCTGTCGGCAAACTGCTGTTTTGA

Upstream 100 bases:

>100_bases
GCCGGCGAATTGAGCGAGGATCCGCAGGACTGGGTGATGCACAAGGTGACGCAGGTGCTCGATGATTATCACGCCGCCTG
CTACCCGGAGGTGCACTGAA

Downstream 100 bases:

>100_bases
GTTTGGGGGAAATCATGTTGACTGATGCGTTATTGATTGCACTGCTGGCCGGGCTGGCGGGGGTCGATCTGTTCGACGGC
CTGACCCACTTCCATCGTCC

Product: PTS system mannose/fructose/sorbose family transporter subunit IIB

Products: NA

Alternate protein names: EIIB-Aga'; PTS system N-acetylgalactosamine-specific EIIB component 2 [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MPNILMTRIDNRLVHGQVGVTWTNTLGANLVVVANDEAAADPVQQNLMDMVVAEGVQTRYFTLQKTIDVIHKAADRQKIF
LVCKTPQDVLRLVQGGVPIRFVNVGNMHFAEGKRQVHKTVSLDEGDVAAFRELAALGVECEVRRVPDEAGEPVGKLLF

Sequences:

>Translated_158_residues
MPNILMTRIDNRLVHGQVGVTWTNTLGANLVVVANDEAAADPVQQNLMDMVVAEGVQTRYFTLQKTIDVIHKAADRQKIF
LVCKTPQDVLRLVQGGVPIRFVNVGNMHFAEGKRQVHKTVSLDEGDVAAFRELAALGVECEVRRVPDEAGEPVGKLLF
>Mature_157_residues
PNILMTRIDNRLVHGQVGVTWTNTLGANLVVVANDEAAADPVQQNLMDMVVAEGVQTRYFTLQKTIDVIHKAADRQKIFL
VCKTPQDVLRLVQGGVPIRFVNVGNMHFAEGKRQVHKTVSLDEGDVAAFRELAALGVECEVRRVPDEAGEPVGKLLF

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI87082217, Length=157, Percent_Identity=64.968152866242, Blast_Score=214, Evalue=2e-57,
Organism=Escherichia coli, GI1789527, Length=153, Percent_Identity=53.5947712418301, Blast_Score=158, Evalue=2e-40,
Organism=Escherichia coli, GI1788120, Length=143, Percent_Identity=43.3566433566434, Blast_Score=115, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17401; Mature: 17270

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNILMTRIDNRLVHGQVGVTWTNTLGANLVVVANDEAAADPVQQNLMDMVVAEGVQTRY
CCCEEEECCCCEEEECCCCEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHH
FTLQKTIDVIHKAADRQKIFLVCKTPQDVLRLVQGGVPIRFVNVGNMHFAEGKRQVHKTV
HHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCEEEEEECCEEHHHHHHHHHHHC
SLDEGDVAAFRELAALGVECEVRRVPDEAGEPVGKLLF
CCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHCC
>Mature Secondary Structure 
PNILMTRIDNRLVHGQVGVTWTNTLGANLVVVANDEAAADPVQQNLMDMVVAEGVQTRY
CCEEEECCCCEEEECCCCEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHH
FTLQKTIDVIHKAADRQKIFLVCKTPQDVLRLVQGGVPIRFVNVGNMHFAEGKRQVHKTV
HHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCEEEEEECCEEHHHHHHHHHHHC
SLDEGDVAAFRELAALGVECEVRRVPDEAGEPVGKLLF
CCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 8932697 [H]