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The map label for this gene is nlpE [H]

Identifier: 157371994

GI number: 157371994

Start: 4156037

End: 4156720

Strand: Reverse

Name: nlpE [H]

Synonym: Spro_3759

Alternate gene names: 157371994

Gene position: 4156720-4156037 (Counterclockwise)

Preceding gene: 157371995

Following gene: 157371987

Centisome position: 76.29

GC content: 53.51

Gene sequence:

>684_bases
GTGAAGAAAATAACACTGGCCCTGTTTTTAGCGGCAGGGGCACTTTCGTTACTGGGATGTAATAACCACTTCCAGCCGAA
AGAACAACCGCTTCAGGCGATGCAACAAAGTTATCAGGGTGTGTTGCCTTGCGCTGACTGCGGCGGTCTGGATACCGCAT
TATTCCTGGATAAAGACGGTACTTTCGTCCTGCAGGAAACCTATCGCGACACCAAAGACGGCGATCAAACCTTTGCCGAC
TACGGTAAATGGGCGCGTACCGCCGATAAGCTGGTGCTGACCGACAGCCGTGGTGATAAGCGTTATTTCCGCCCGGTGGG
TAAAAGTTTGGAAATGCTGGATCAAACCGGCATGCCGATTGAGTCGCAGTTGAATTACCGTCTTGATCCGGTAGAGAAAG
CCTTGCCTAAAACGCCGATGGCGCTGAAAGGCAGCTACACCTACATGGCGGATGCGGCCGTGTTCAAAGATTGCGCCACC
GGTAAAACGTTCCCGGTCGACAATACAATCGCGCTGGAGCAGGGGTATGCCAAGGCGTACAAAACGCCGGGCGAGCCGGT
GTTCCTGACGCTGAATGGCCACTTTAGCGTTCAGCCGTCAATGGAAGAGGGGTTAACCGAGAAGGCGCTGGTGCCGGACG
GTAAAGTAGCCTTCGACCGCAGTAAAAATTGCGACAGCAAATAA

Upstream 100 bases:

>100_bases
AATTACGTCGTCTGGAAGGCAAGGTACGGAAAGGCGCGACTAAAGCGCTGCGCGGCAAGATCCGCACTTAAGGACACATG
AACACAGTAGGAGAATAACT

Downstream 100 bases:

>100_bases
CCGCTACAAAAAAACCCGCCTAGGCGGGTTTTTAGTTTCTGCGATAGTGGATTAGCGCTTGATCTGCCCCAGCAAGAAAT
CAATGACTTCGCCGGTTTTA

Product: hypothetical protein

Products: NA

Alternate protein names: Copper homeostasis protein CutF [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MKKITLALFLAAGALSLLGCNNHFQPKEQPLQAMQQSYQGVLPCADCGGLDTALFLDKDGTFVLQETYRDTKDGDQTFAD
YGKWARTADKLVLTDSRGDKRYFRPVGKSLEMLDQTGMPIESQLNYRLDPVEKALPKTPMALKGSYTYMADAAVFKDCAT
GKTFPVDNTIALEQGYAKAYKTPGEPVFLTLNGHFSVQPSMEEGLTEKALVPDGKVAFDRSKNCDSK

Sequences:

>Translated_227_residues
MKKITLALFLAAGALSLLGCNNHFQPKEQPLQAMQQSYQGVLPCADCGGLDTALFLDKDGTFVLQETYRDTKDGDQTFAD
YGKWARTADKLVLTDSRGDKRYFRPVGKSLEMLDQTGMPIESQLNYRLDPVEKALPKTPMALKGSYTYMADAAVFKDCAT
GKTFPVDNTIALEQGYAKAYKTPGEPVFLTLNGHFSVQPSMEEGLTEKALVPDGKVAFDRSKNCDSK
>Mature_227_residues
MKKITLALFLAAGALSLLGCNNHFQPKEQPLQAMQQSYQGVLPCADCGGLDTALFLDKDGTFVLQETYRDTKDGDQTFAD
YGKWARTADKLVLTDSRGDKRYFRPVGKSLEMLDQTGMPIESQLNYRLDPVEKALPKTPMALKGSYTYMADAAVFKDCAT
GKTFPVDNTIALEQGYAKAYKTPGEPVFLTLNGHFSVQPSMEEGLTEKALVPDGKVAFDRSKNCDSK

Specific function: Involved in copper homeostasis. Could be involved in both copper efflux and the delivery of copper to copper-dependent enzymes. When overproduced induces degP through the activation of the two-component system CpxA/CpxR [H]

COG id: COG3015

COG function: function code MP; Uncharacterized lipoprotein NlpE involved in copper resistance

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786390, Length=232, Percent_Identity=46.9827586206897, Blast_Score=222, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007298 [H]

Pfam domain/function: PF04170 NlpE [H]

EC number: NA

Molecular weight: Translated: 24988; Mature: 24988

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKITLALFLAAGALSLLGCNNHFQPKEQPLQAMQQSYQGVLPCADCGGLDTALFLDKDG
CCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCC
TFVLQETYRDTKDGDQTFADYGKWARTADKLVLTDSRGDKRYFRPVGKSLEMLDQTGMPI
CEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCH
ESQLNYRLDPVEKALPKTPMALKGSYTYMADAAVFKDCATGKTFPVDNTIALEQGYAKAY
HHCCCCCCCHHHHHCCCCCCEECCCCCEEHHHHHHHHHCCCCCCCCCCEEECCCCHHHHH
KTPGEPVFLTLNGHFSVQPSMEEGLTEKALVPDGKVAFDRSKNCDSK
CCCCCEEEEEECCCEEECCCHHHCCCCCCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure
MKKITLALFLAAGALSLLGCNNHFQPKEQPLQAMQQSYQGVLPCADCGGLDTALFLDKDG
CCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCC
TFVLQETYRDTKDGDQTFADYGKWARTADKLVLTDSRGDKRYFRPVGKSLEMLDQTGMPI
CEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCH
ESQLNYRLDPVEKALPKTPMALKGSYTYMADAAVFKDCATGKTFPVDNTIALEQGYAKAY
HHCCCCCCCHHHHHCCCCCCEECCCCCEEHHHHHHHHHCCCCCCCCCCEEECCCCHHHHH
KTPGEPVFLTLNGHFSVQPSMEEGLTEKALVPDGKVAFDRSKNCDSK
CCCCCEEEEEECCCEEECCCHHHCCCCCCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7635808; 7635807; 9278503 [H]