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The map label for this gene is grpE

Identifier: 157371919

GI number: 157371919

Start: 4076625

End: 4077197

Strand: Reverse

Name: grpE

Synonym: Spro_3684

Alternate gene names: 157371919

Gene position: 4077197-4076625 (Counterclockwise)

Preceding gene: 157371923

Following gene: 157371917

Centisome position: 74.83

GC content: 55.32

Gene sequence:

>573_bases
ATGAGTAGTAAAGAACAGAAGACGCCAAACGAGCAAGTCTCGGAAGAAATGGAACAGCAGGAAGTCTTGCCGGAAGCGGC
TGAGGGCGTCGATCTGCGGGATGAGCGCATTGCTGAGCTGGAAGCTCAACTGGCCGAAGCCCAGCAGCATGAACGCGACA
GCCTGCTGCGCGCCAAAGCGGAAATGGAAAACGTCCGCCGCCGCACCGAGCTGGATATTGAAAAAGCGCACAAGTTTGCT
CTGGAGAAATTCTCCGGTGACCTGCTGCCGGTGTTGGATAACCTGGAGCGTGCGTTGGAACTGGCGGATAAAAACAATCC
GGAGCTGACGGCGATGATCGAAGGTATCGAGCTGACGCTGAAGTCCCTGCAGGACGTAGTGCGTAAGTACGGTATCGAAA
TCGTTGGCGACACTAACGTGCCGTTCAACCCGGAAGTACACCAGGCAATGAGCCTGATGGAATCAGCCGATCACCAGCCA
AATCACGTAATGATGGTGATGCAAAAAGGCTATACGCTGAATGGCCGCCTGCTGCGCCCGGCGATGGTCGCGGTTTCCAA
AGCCAAGGCTTAA

Upstream 100 bases:

>100_bases
CAGTTTATGCCCCACATTGTGACCGGTTGACTTGAAACCCCGGTTTTGATCCCCATAATAAGCCAAGTTGCGAGATTAAT
GCTAAAACGCGGAGATATTC

Downstream 100 bases:

>100_bases
GCGAATTAAGTAAAGAAAAGGCACCTTAGGGTGCCTTTTTTACGTCTGAATCAGCTCTGCGGCAGTTGCGGCAGCCAGTC
GATAGGTTGCAACCCCTGTT

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MSSKEQKTPNEQVSEEMEQQEVLPEAAEGVDLRDERIAELEAQLAEAQQHERDSLLRAKAEMENVRRRTELDIEKAHKFA
LEKFSGDLLPVLDNLERALELADKNNPELTAMIEGIELTLKSLQDVVRKYGIEIVGDTNVPFNPEVHQAMSLMESADHQP
NHVMMVMQKGYTLNGRLLRPAMVAVSKAKA

Sequences:

>Translated_190_residues
MSSKEQKTPNEQVSEEMEQQEVLPEAAEGVDLRDERIAELEAQLAEAQQHERDSLLRAKAEMENVRRRTELDIEKAHKFA
LEKFSGDLLPVLDNLERALELADKNNPELTAMIEGIELTLKSLQDVVRKYGIEIVGDTNVPFNPEVHQAMSLMESADHQP
NHVMMVMQKGYTLNGRLLRPAMVAVSKAKA
>Mature_189_residues
SSKEQKTPNEQVSEEMEQQEVLPEAAEGVDLRDERIAELEAQLAEAQQHERDSLLRAKAEMENVRRRTELDIEKAHKFAL
EKFSGDLLPVLDNLERALELADKNNPELTAMIEGIELTLKSLQDVVRKYGIEIVGDTNVPFNPEVHQAMSLMESADHQPN
HVMMVMQKGYTLNGRLLRPAMVAVSKAKA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=194, Percent_Identity=32.9896907216495, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI40255109, Length=137, Percent_Identity=33.5766423357664, Blast_Score=73, Evalue=1e-13,
Organism=Escherichia coli, GI1788967, Length=197, Percent_Identity=67.005076142132, Blast_Score=259, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI17552458, Length=141, Percent_Identity=34.7517730496454, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324806, Length=167, Percent_Identity=35.3293413173653, Blast_Score=101, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24653432, Length=159, Percent_Identity=33.9622641509434, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_SERP5 (A8GI40)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479908.1
- ProteinModelPortal:   A8GI40
- SMR:   A8GI40
- STRING:   A8GI40
- GeneID:   5606930
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3684
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- ProtClustDB:   PRK14150
- BioCyc:   SPRO399741:SPRO_3684-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21458; Mature: 21327

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKEQKTPNEQVSEEMEQQEVLPEAAEGVDLRDERIAELEAQLAEAQQHERDSLLRAKA
CCCCCCCCCHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMENVRRRTELDIEKAHKFALEKFSGDLLPVLDNLERALELADKNNPELTAMIEGIELTL
HHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
KSLQDVVRKYGIEIVGDTNVPFNPEVHQAMSLMESADHQPNHVMMVMQKGYTLNGRLLRP
HHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHH
AMVAVSKAKA
HHHHHHHCCC
>Mature Secondary Structure 
SSKEQKTPNEQVSEEMEQQEVLPEAAEGVDLRDERIAELEAQLAEAQQHERDSLLRAKA
CCCCCCCCHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMENVRRRTELDIEKAHKFALEKFSGDLLPVLDNLERALELADKNNPELTAMIEGIELTL
HHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
KSLQDVVRKYGIEIVGDTNVPFNPEVHQAMSLMESADHQPNHVMMVMQKGYTLNGRLLRP
HHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHH
AMVAVSKAKA
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA