Click here to switch to the map view.

The map label for this gene is thiD [H]

Identifier: 157371812

GI number: 157371812

Start: 3952788

End: 3953588

Strand: Reverse

Name: thiD [H]

Synonym: Spro_3577

Alternate gene names: 157371812

Gene position: 3953588-3952788 (Counterclockwise)

Preceding gene: 157371813

Following gene: 157371811

Centisome position: 72.56

GC content: 62.05

Gene sequence:

>801_bases
ATGAAACGGATTAACGCGTTGACCATCGCCGGTACCGATCCGAGCGGGGGGGCCGGCATCCAGGCCGACTTGAAAGCCTT
CTCCGCGCTGGGGGCCTACGGTGCCTCGGTAATCACCGCGCTGGTGGCGCAAAATACCCGCGGCGTGCAGTCGGTGTATA
ACATCGAACCGGCTTTTGTCGCTGCCCAGCTTGATTCCGTGTTCAGCGATTTGCGTATCGACAGCACCAAGATTGGCATG
TTGGCCAACGCCGATATTGTGCAGGCGGTCGCGGAGCGTTTACGTCACTATCAGCCGTCGTTTGTGGTACTGGATACGGT
GATGCTGGCGAAAAGCGGCGATCCTCTGCTGGCGCCGGAGGCGGTGGAATCGATTCGCTGCGAGCTGTTGCCGCTGGTGT
CGATCATCACGCCGAACCTGCCGGAAGCGGCGGCCCTGCTGGAATGCGCCCCGGCGGAAAATGAACGCCAGATGCGTGAG
CAGGGGCGAGCACTGTTGGCGATGGGTTGCCAGGCGGTATTGATGAAGGGCGGCCATCTTAGCGAAAGTGAAAGCCCGGA
CTGGCTGTTCAGTGCGGGACTGGAGCAACGCTTTACTGCACCGCGCGTAGCGACCCGCCATACGCATGGCACCGGCTGCA
CGCTGTCTGCCGCATTGGCCGCCCTGCGTCCGCGTCACGACGACTGGGCAGGCACGGTGGCATCCGCCAAGGATTATCTG
CAAAAAGCCTTGCAGCAGGCCGATACGCTGGAAGTGGGGCACGGCATCGGGCCGGTGCATCATTTTCACGCCTGGTGGTG
A

Upstream 100 bases:

>100_bases
CACATTGCGGTGAGATGGCCACGCGCCGCGCTGCCGGGCCGGGCAGTTTTACCCCGGCATTCCTCGACGCGCTGTACCAA
CTGCGCCCGGAGGATCTGCA

Downstream 100 bases:

>100_bases
GCCAACTTTTTGCTACCCTTGTGGTCTGACTAGATCCAGGCCCGGTATTGGCCGGGTGCGATTAAATTTTATCAAGGGAG
AAGGCCATGACAGGACAAAA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKRINALTIAGTDPSGGAGIQADLKAFSALGAYGASVITALVAQNTRGVQSVYNIEPAFVAAQLDSVFSDLRIDSTKIGM
LANADIVQAVAERLRHYQPSFVVLDTVMLAKSGDPLLAPEAVESIRCELLPLVSIITPNLPEAAALLECAPAENERQMRE
QGRALLAMGCQAVLMKGGHLSESESPDWLFSAGLEQRFTAPRVATRHTHGTGCTLSAALAALRPRHDDWAGTVASAKDYL
QKALQQADTLEVGHGIGPVHHFHAWW

Sequences:

>Translated_266_residues
MKRINALTIAGTDPSGGAGIQADLKAFSALGAYGASVITALVAQNTRGVQSVYNIEPAFVAAQLDSVFSDLRIDSTKIGM
LANADIVQAVAERLRHYQPSFVVLDTVMLAKSGDPLLAPEAVESIRCELLPLVSIITPNLPEAAALLECAPAENERQMRE
QGRALLAMGCQAVLMKGGHLSESESPDWLFSAGLEQRFTAPRVATRHTHGTGCTLSAALAALRPRHDDWAGTVASAKDYL
QKALQQADTLEVGHGIGPVHHFHAWW
>Mature_266_residues
MKRINALTIAGTDPSGGAGIQADLKAFSALGAYGASVITALVAQNTRGVQSVYNIEPAFVAAQLDSVFSDLRIDSTKIGM
LANADIVQAVAERLRHYQPSFVVLDTVMLAKSGDPLLAPEAVESIRCELLPLVSIITPNLPEAAALLECAPAENERQMRE
QGRALLAMGCQAVLMKGGHLSESESPDWLFSAGLEQRFTAPRVATRHTHGTGCTLSAALAALRPRHDDWAGTVASAKDYL
QKALQQADTLEVGHGIGPVHHFHAWW

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=266, Percent_Identity=78.9473684210526, Blast_Score=429, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6325378, Length=277, Percent_Identity=30.6859205776173, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324997, Length=272, Percent_Identity=36.0294117647059, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324517, Length=273, Percent_Identity=37.3626373626374, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28369; Mature: 28369

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRINALTIAGTDPSGGAGIQADLKAFSALGAYGASVITALVAQNTRGVQSVYNIEPAFV
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHH
AAQLDSVFSDLRIDSTKIGMLANADIVQAVAERLRHYQPSFVVLDTVMLAKSGDPLLAPE
HHHHHHHHHHHCCCCHHCCEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHH
AVESIRCELLPLVSIITPNLPEAAALLECAPAENERQMREQGRALLAMGCQAVLMKGGHL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHCCCCC
SESESPDWLFSAGLEQRFTAPRVATRHTHGTGCTLSAALAALRPRHDDWAGTVASAKDYL
CCCCCCCHHHHCCHHHHCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
QKALQQADTLEVGHGIGPVHHFHAWW
HHHHHHHHHHHHCCCCCCCCHHCCCC
>Mature Secondary Structure
MKRINALTIAGTDPSGGAGIQADLKAFSALGAYGASVITALVAQNTRGVQSVYNIEPAFV
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHH
AAQLDSVFSDLRIDSTKIGMLANADIVQAVAERLRHYQPSFVVLDTVMLAKSGDPLLAPE
HHHHHHHHHHHCCCCHHCCEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHH
AVESIRCELLPLVSIITPNLPEAAALLECAPAENERQMREQGRALLAMGCQAVLMKGGHL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHCCCCC
SESESPDWLFSAGLEQRFTAPRVATRHTHGTGCTLSAALAALRPRHDDWAGTVASAKDYL
CCCCCCCHHHHCCHHHHCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
QKALQQADTLEVGHGIGPVHHFHAWW
HHHHHHHHHHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]