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The map label for this gene is thiM

Identifier: 157371813

GI number: 157371813

Start: 3953585

End: 3954379

Strand: Reverse

Name: thiM

Synonym: Spro_3578

Alternate gene names: 157371813

Gene position: 3954379-3953585 (Counterclockwise)

Preceding gene: 157371815

Following gene: 157371812

Centisome position: 72.57

GC content: 64.28

Gene sequence:

>795_bases
ATGCTCGCTCGACCTGATGTTTTCCCCGGCGCCCGCGCCGCGGCCTGTTTAACCCAATTTAAACGCCAATCCCCATTGAT
TCACTGCCTGACCAACGAAGTGGTGCAGGAGCTGACGGCTAACGTGTTGCTGGCGCTCGGTGCTTCCCCGGCGATGGTGG
TTGAGCCGACCGAAGCGGCACAGTTCAGCCGTTTGGCAGACGCTTTGTTGATCAATATCGGCACCCTCAATGCATCACGC
GCCGAATCCATGCTGGCGGCGATAGAAGCTGCCAATGCGGCAGGCACGCCCTGGACGCTGGATCCGGTCGCCGTCGGTGG
GCTGGCGTATCGCACCGCCTTTGCCCAAAGTTTGCTGGGCGAAAAGCCGGCGGCAATCCGCGGCAATGCTTCTGAAATTA
TGGCGCTGAGTGGCCTGCAGGCGAGTGGGCGCGGCGTCGACAGCGCTGACGATTCACTGGCGGCGTTACCGGCGGCGCGC
GAATTGGCACGAAACAGTGGGGCCGTGGTGGCGGTGACCGGCGTGGTGGATTACATCACCGACGGCCAGCGTGACTGGGC
GGTTGCCGGGGGCGACGTGCTGATGACGCGAGTGGTGGGTACCGGTTGCGCACTTTCGGCCGTGGTGGCGGCATTTTGCA
GCCTGCCGGGCGATCGGTTGGACAACGTGGCGACCGCCTGCCGGGTGATGTCACATTGCGGTGAGATGGCCACGCGCCGC
GCTGCCGGGCCGGGCAGTTTTACCCCGGCATTCCTCGACGCGCTGTACCAACTGCGCCCGGAGGATCTGCAATGA

Upstream 100 bases:

>100_bases
TGAGATTTTACCCGTATTACCTGATCTGGATTATGCCAGCGTAGGGAAGTCTCGGTGCCCCAACCGGTACCCGCCTTCTT
GAACGCCGGTTGGGAGGAAT

Downstream 100 bases:

>100_bases
AACGGATTAACGCGTTGACCATCGCCGGTACCGATCCGAGCGGGGGGGCCGGCATCCAGGCCGACTTGAAAGCCTTCTCC
GCGCTGGGGGCCTACGGTGC

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MLARPDVFPGARAAACLTQFKRQSPLIHCLTNEVVQELTANVLLALGASPAMVVEPTEAAQFSRLADALLINIGTLNASR
AESMLAAIEAANAAGTPWTLDPVAVGGLAYRTAFAQSLLGEKPAAIRGNASEIMALSGLQASGRGVDSADDSLAALPAAR
ELARNSGAVVAVTGVVDYITDGQRDWAVAGGDVLMTRVVGTGCALSAVVAAFCSLPGDRLDNVATACRVMSHCGEMATRR
AAGPGSFTPAFLDALYQLRPEDLQ

Sequences:

>Translated_264_residues
MLARPDVFPGARAAACLTQFKRQSPLIHCLTNEVVQELTANVLLALGASPAMVVEPTEAAQFSRLADALLINIGTLNASR
AESMLAAIEAANAAGTPWTLDPVAVGGLAYRTAFAQSLLGEKPAAIRGNASEIMALSGLQASGRGVDSADDSLAALPAAR
ELARNSGAVVAVTGVVDYITDGQRDWAVAGGDVLMTRVVGTGCALSAVVAAFCSLPGDRLDNVATACRVMSHCGEMATRR
AAGPGSFTPAFLDALYQLRPEDLQ
>Mature_264_residues
MLARPDVFPGARAAACLTQFKRQSPLIHCLTNEVVQELTANVLLALGASPAMVVEPTEAAQFSRLADALLINIGTLNASR
AESMLAAIEAANAAGTPWTLDPVAVGGLAYRTAFAQSLLGEKPAAIRGNASEIMALSGLQASGRGVDSADDSLAALPAAR
ELARNSGAVVAVTGVVDYITDGQRDWAVAGGDVLMTRVVGTGCALSAVVAAFCSLPGDRLDNVATACRVMSHCGEMATRR
AAGPGSFTPAFLDALYQLRPEDLQ

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=256, Percent_Identity=61.71875, Blast_Score=310, Evalue=6e-86,
Organism=Saccharomyces cerevisiae, GI6325042, Length=275, Percent_Identity=31.6363636363636, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_SERP5 (A8GHT4)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479802.1
- ProteinModelPortal:   A8GHT4
- SMR:   A8GHT4
- STRING:   A8GHT4
- GeneID:   5605594
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3578
- eggNOG:   COG2145
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- ProtClustDB:   PRK09355
- BioCyc:   SPRO399741:SPRO_3578-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 27151; Mature: 27151

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: BINDING 52-52 BINDING 127-127 BINDING 173-173 BINDING 200-200

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLARPDVFPGARAAACLTQFKRQSPLIHCLTNEVVQELTANVLLALGASPAMVVEPTEAA
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHH
QFSRLADALLINIGTLNASRAESMLAAIEAANAAGTPWTLDPVAVGGLAYRTAFAQSLLG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
EKPAAIRGNASEIMALSGLQASGRGVDSADDSLAALPAARELARNSGAVVAVTGVVDYIT
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHH
DGQRDWAVAGGDVLMTRVVGTGCALSAVVAAFCSLPGDRLDNVATACRVMSHCGEMATRR
CCCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AAGPGSFTPAFLDALYQLRPEDLQ
CCCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLARPDVFPGARAAACLTQFKRQSPLIHCLTNEVVQELTANVLLALGASPAMVVEPTEAA
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHH
QFSRLADALLINIGTLNASRAESMLAAIEAANAAGTPWTLDPVAVGGLAYRTAFAQSLLG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
EKPAAIRGNASEIMALSGLQASGRGVDSADDSLAALPAARELARNSGAVVAVTGVVDYIT
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHH
DGQRDWAVAGGDVLMTRVVGTGCALSAVVAAFCSLPGDRLDNVATACRVMSHCGEMATRR
CCCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AAGPGSFTPAFLDALYQLRPEDLQ
CCCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA