Click here to switch to the map view.

The map label for this gene is yfgF [H]

Identifier: 157371778

GI number: 157371778

Start: 3913901

End: 3916153

Strand: Reverse

Name: yfgF [H]

Synonym: Spro_3543

Alternate gene names: 157371778

Gene position: 3916153-3913901 (Counterclockwise)

Preceding gene: 157371782

Following gene: 157371775

Centisome position: 71.87

GC content: 54.86

Gene sequence:

>2253_bases
ATGGAATTGCGCCCTGACGGACTCGTTATTTTTATGGTTAAGCCAATATTCTGGAAGCATTTACGCGAGCGCTGGTGGGG
TTATCCCCTCATCTGGGGCTTGTTGTTGATCCCGTTCGCCGGTTGGTTGTCGCCGCGCATTATGTTGCCAGAGGGCAAAG
TCTATCTGCTCTATCTGCCACTGGCAGCCTGCATTGCGTTGTTGATGGTCTACGACTGGCGGGCGCTGCCGGGCATTACC
CTTGCCATCATGGTGCGATACGCGAGCCGACTGGGGCTGGAGAGCGGCATATTGGTCACCTCGTTGTACCTGCTTTGTCT
GATCCTGTGCTGGTTGGGCTATCGCAGCCAGACTCAGCGTCGTTGGTGTGCGGCCCCCGGCGTACCGACATTGGTCAAAG
CGAGGTTGATCTGGTTGGTGGTGCTGCCGGCGTTGCTGTTTGTCTTCGGCCTGAAACTGCTGGTCGCTCTGGCCCAATTG
CCTGACGAACTGGGGATGATGCCCGGTAATAGTGATTATTTGTTGGCCCTGGTCAATTTTCAGGCGCTGCTGCTGGGTTG
TCTGTCCGCTATGCCGCTGTTCTACTTTATGTTGCGTCTATTGAACAAGCCCAGGTTCGCACTGACGCTGTGGCGACGTT
TTGGCCGTGAAATGGCCCCCCAGGCCGATAAAAAAGAGTTGTCTCTGTGGTTGCTGGTGCTGGTCAGCATGGTGGCCTTG
CTGTCTTACTACGCCAAAAATTCCAATCCTCTGCTGCTGGGGGGCTATGGCCTGATCCTGTTGCTGCCGGTGATGCTGTA
TGGCGCGATGCGCTTTGGCTATCAATTCAACTGCATTATCTGGTCTTCTTCGCTGCTGGTGCTGTTTTTCCATTATCCCG
GTTATGCGCAGCGCGACAATTTATTGCATAACCTGGCAGTCATCTCTTCGATGATGGTGGTGTTTACCCTGTGTATTTAC
CTGATGTCGGCGCTCAATACTCGCCAGCGATTAAGTCATGCCAGGGCGCAAAGCGCTGCGCTGCAAGATCCGGTGATTGG
CTTGCCTAACCTGCGCGCCCTCAAGCGCGATCTGACCTTGTCATTACCTTCAACGCTGTGTTTCCTGCGCGTTAGCGAGC
TGGATTTGCTGAGCCGCAACTACGGTATGCAATTGCGCATTCGTTTCAAACAGCAGTTGGCGGCGCTGCTGCATCAGGTA
TTGGCACCCGGTGAGGGCGTATATCATCTGCCGGGTTATGATCTGTTGCTGCGGGTGGATGGCGAAGACAGCGAAGAAAA
GGTTTTGCAGATCTACCACGCGCTGGAGAAATTCCGTCTGATATGGAACGGGTTGCCGCTCCATCCGCCGCTGGGGTTAG
GCTATTGCAGCGTGAATGCGGATTTCGAACATCTCTACCTTCTGCTGGGGGAGCTTAGCTCGTTGGCCGAAGTATCGTTG
ACCAGCGGCAAACCGGAGAGCGTGCAAAACGGTGGGCAATGGGTACAGGAAGAAATTAAGCGCAAAGTCGAGTTACTGCA
TGGTGTACAGCATGCGTTGGATAACGATGGGTTCATTCTGATGGCGCAGCCGATCGTGGGCCTGCGCGGTGACCGATATC
ATGAAATTCTACTGCGCATGCGCGATACGTGCGGAGAGATCATATCGCCTAATGATTTTCTGCCGGTGGCACATGAGTTC
GGCCTGGCGTATCGGCTGGACCTGTGGGTGTTGCGCAATACGCTGAAATTTATGGATCTGTACCGTGCCGAACTGCCCAG
TGCGCGTTTCGCGGTTAATCTGTCACCTTCTTCGCTGTGCCGGCCTATGCTGCGCCACGATGTCAACAGCCTGCTGGAGC
AGTACCAGATTGAGCCCTATCAATTGACGCTGGAAGTGACCGAATCGCATCTGTTGCAGGATACGGCCTATGCCGGGGCC
AATTTGCAGGCACTGCGTGAGATAGGGTGTCGCATTGCGCTGGATGACTTCGGTACCGGCTATGCCAGCTACGATCGGCT
GAAGATGCTGCCGGTCGACATGTTGAAAATCGACGGCAGTTTCGTGCGTGACATGCTGACCAATCCGGTGGATTTCCAGG
TCATTACGTCTATCTGCAAGGTTGCGCGCATGAAACGGCTGGCGATAGTGGCTGAATATGTGGAAAGCATGGAGCAGTTG
GTCGCGCTGAAAGGGGTTGGCGTGGACTATATGCAGGGCTACTTCATTGGCGAGCCACAACCCTTGCTGGCGCTGTTACC
GACACCGCGTTGA

Upstream 100 bases:

>100_bases
AAATTCCACTCCATTCTCCTGTCGCATATGTTACTTTCACTTTGCCTCATGAATGGATAGAGGTACGTAAAGGAACCTGC
CTTATTACCTGAACGCGCGA

Downstream 100 bases:

>100_bases
GTGTTGCCCCCCGGCAGGCAGGGGGCGAAAGACTCAGGCAGTACGTTGCTCGTTTTGGGTCTCTTCTTCTTCGCAAATCA
GCTTCCAGCCGACGACGTCA

Product: diguanylate cyclase/phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 750; Mature: 750

Protein sequence:

>750_residues
MELRPDGLVIFMVKPIFWKHLRERWWGYPLIWGLLLIPFAGWLSPRIMLPEGKVYLLYLPLAACIALLMVYDWRALPGIT
LAIMVRYASRLGLESGILVTSLYLLCLILCWLGYRSQTQRRWCAAPGVPTLVKARLIWLVVLPALLFVFGLKLLVALAQL
PDELGMMPGNSDYLLALVNFQALLLGCLSAMPLFYFMLRLLNKPRFALTLWRRFGREMAPQADKKELSLWLLVLVSMVAL
LSYYAKNSNPLLLGGYGLILLLPVMLYGAMRFGYQFNCIIWSSSLLVLFFHYPGYAQRDNLLHNLAVISSMMVVFTLCIY
LMSALNTRQRLSHARAQSAALQDPVIGLPNLRALKRDLTLSLPSTLCFLRVSELDLLSRNYGMQLRIRFKQQLAALLHQV
LAPGEGVYHLPGYDLLLRVDGEDSEEKVLQIYHALEKFRLIWNGLPLHPPLGLGYCSVNADFEHLYLLLGELSSLAEVSL
TSGKPESVQNGGQWVQEEIKRKVELLHGVQHALDNDGFILMAQPIVGLRGDRYHEILLRMRDTCGEIISPNDFLPVAHEF
GLAYRLDLWVLRNTLKFMDLYRAELPSARFAVNLSPSSLCRPMLRHDVNSLLEQYQIEPYQLTLEVTESHLLQDTAYAGA
NLQALREIGCRIALDDFGTGYASYDRLKMLPVDMLKIDGSFVRDMLTNPVDFQVITSICKVARMKRLAIVAEYVESMEQL
VALKGVGVDYMQGYFIGEPQPLLALLPTPR

Sequences:

>Translated_750_residues
MELRPDGLVIFMVKPIFWKHLRERWWGYPLIWGLLLIPFAGWLSPRIMLPEGKVYLLYLPLAACIALLMVYDWRALPGIT
LAIMVRYASRLGLESGILVTSLYLLCLILCWLGYRSQTQRRWCAAPGVPTLVKARLIWLVVLPALLFVFGLKLLVALAQL
PDELGMMPGNSDYLLALVNFQALLLGCLSAMPLFYFMLRLLNKPRFALTLWRRFGREMAPQADKKELSLWLLVLVSMVAL
LSYYAKNSNPLLLGGYGLILLLPVMLYGAMRFGYQFNCIIWSSSLLVLFFHYPGYAQRDNLLHNLAVISSMMVVFTLCIY
LMSALNTRQRLSHARAQSAALQDPVIGLPNLRALKRDLTLSLPSTLCFLRVSELDLLSRNYGMQLRIRFKQQLAALLHQV
LAPGEGVYHLPGYDLLLRVDGEDSEEKVLQIYHALEKFRLIWNGLPLHPPLGLGYCSVNADFEHLYLLLGELSSLAEVSL
TSGKPESVQNGGQWVQEEIKRKVELLHGVQHALDNDGFILMAQPIVGLRGDRYHEILLRMRDTCGEIISPNDFLPVAHEF
GLAYRLDLWVLRNTLKFMDLYRAELPSARFAVNLSPSSLCRPMLRHDVNSLLEQYQIEPYQLTLEVTESHLLQDTAYAGA
NLQALREIGCRIALDDFGTGYASYDRLKMLPVDMLKIDGSFVRDMLTNPVDFQVITSICKVARMKRLAIVAEYVESMEQL
VALKGVGVDYMQGYFIGEPQPLLALLPTPR
>Mature_750_residues
MELRPDGLVIFMVKPIFWKHLRERWWGYPLIWGLLLIPFAGWLSPRIMLPEGKVYLLYLPLAACIALLMVYDWRALPGIT
LAIMVRYASRLGLESGILVTSLYLLCLILCWLGYRSQTQRRWCAAPGVPTLVKARLIWLVVLPALLFVFGLKLLVALAQL
PDELGMMPGNSDYLLALVNFQALLLGCLSAMPLFYFMLRLLNKPRFALTLWRRFGREMAPQADKKELSLWLLVLVSMVAL
LSYYAKNSNPLLLGGYGLILLLPVMLYGAMRFGYQFNCIIWSSSLLVLFFHYPGYAQRDNLLHNLAVISSMMVVFTLCIY
LMSALNTRQRLSHARAQSAALQDPVIGLPNLRALKRDLTLSLPSTLCFLRVSELDLLSRNYGMQLRIRFKQQLAALLHQV
LAPGEGVYHLPGYDLLLRVDGEDSEEKVLQIYHALEKFRLIWNGLPLHPPLGLGYCSVNADFEHLYLLLGELSSLAEVSL
TSGKPESVQNGGQWVQEEIKRKVELLHGVQHALDNDGFILMAQPIVGLRGDRYHEILLRMRDTCGEIISPNDFLPVAHEF
GLAYRLDLWVLRNTLKFMDLYRAELPSARFAVNLSPSSLCRPMLRHDVNSLLEQYQIEPYQLTLEVTESHLLQDTAYAGA
NLQALREIGCRIALDDFGTGYASYDRLKMLPVDMLKIDGSFVRDMLTNPVDFQVITSICKVARMKRLAIVAEYVESMEQL
VALKGVGVDYMQGYFIGEPQPLLALLPTPR

Specific function: Truncated proteins consisting of the GGDEF/EAL domains (residues 319-747) or of the EAL domain alone (481-747) have c-di- GMP phosphodiesterase activity. They do not have diguanylate cyclase activity. Cyclic-di-GMP is a second messenger which controls cel

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=737, Percent_Identity=43.5549525101764, Blast_Score=620, Evalue=1e-178,
Organism=Escherichia coli, GI87082096, Length=715, Percent_Identity=30.2097902097902, Blast_Score=343, Evalue=2e-95,
Organism=Escherichia coli, GI1790496, Length=235, Percent_Identity=33.6170212765957, Blast_Score=131, Evalue=2e-31,
Organism=Escherichia coli, GI1787541, Length=239, Percent_Identity=31.7991631799163, Blast_Score=130, Evalue=4e-31,
Organism=Escherichia coli, GI87081921, Length=439, Percent_Identity=26.879271070615, Blast_Score=123, Evalue=5e-29,
Organism=Escherichia coli, GI87081743, Length=238, Percent_Identity=32.7731092436975, Blast_Score=122, Evalue=1e-28,
Organism=Escherichia coli, GI226510982, Length=362, Percent_Identity=30.1104972375691, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI87081845, Length=233, Percent_Identity=31.7596566523605, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1786507, Length=255, Percent_Identity=29.0196078431373, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI87081980, Length=232, Percent_Identity=34.051724137931, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1788502, Length=241, Percent_Identity=29.045643153527, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1787055, Length=252, Percent_Identity=26.984126984127, Blast_Score=95, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895 [H]

Pfam domain/function: PF00563 EAL; PF05231 MASE1 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 85080; Mature: 85080

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRPDGLVIFMVKPIFWKHLRERWWGYPLIWGLLLIPFAGWLSPRIMLPEGKVYLLYLP
CCCCCCCEEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCCEEEEEHH
LAACIALLMVYDWRALPGITLAIMVRYASRLGLESGILVTSLYLLCLILCWLGYRSQTQR
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHH
RWCAAPGVPTLVKARLIWLVVLPALLFVFGLKLLVALAQLPDELGMMPGNSDYLLALVNF
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCEEHHHHH
QALLLGCLSAMPLFYFMLRLLNKPRFALTLWRRFGREMAPQADKKELSLWLLVLVSMVAL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHH
LSYYAKNSNPLLLGGYGLILLLPVMLYGAMRFGYQFNCIIWSSSLLVLFFHYPGYAQRDN
HHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEEEEECCCCCCHHH
LLHNLAVISSMMVVFTLCIYLMSALNTRQRLSHARAQSAALQDPVIGLPNLRALKRDLTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
SLPSTLCFLRVSELDLLSRNYGMQLRIRFKQQLAALLHQVLAPGEGVYHLPGYDLLLRVD
CCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEC
GEDSEEKVLQIYHALEKFRLIWNGLPLHPPLGLGYCSVNADFEHLYLLLGELSSLAEVSL
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHC
TSGKPESVQNGGQWVQEEIKRKVELLHGVQHALDNDGFILMAQPIVGLRGDRYHEILLRM
CCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHH
RDTCGEIISPNDFLPVAHEFGLAYRLDLWVLRNTLKFMDLYRAELPSARFAVNLSPSSLC
HHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHH
RPMLRHDVNSLLEQYQIEPYQLTLEVTESHLLQDTAYAGANLQALREIGCRIALDDFGTG
HHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHCCCCHHHHHHCCCEEEECCCCCC
YASYDRLKMLPVDMLKIDGSFVRDMLTNPVDFQVITSICKVARMKRLAIVAEYVESMEQL
CCCCCHHEECCHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VALKGVGVDYMQGYFIGEPQPLLALLPTPR
HHHHCCCHHHHCCEECCCCCCCEEECCCCC
>Mature Secondary Structure
MELRPDGLVIFMVKPIFWKHLRERWWGYPLIWGLLLIPFAGWLSPRIMLPEGKVYLLYLP
CCCCCCCEEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCCEEEEEHH
LAACIALLMVYDWRALPGITLAIMVRYASRLGLESGILVTSLYLLCLILCWLGYRSQTQR
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHH
RWCAAPGVPTLVKARLIWLVVLPALLFVFGLKLLVALAQLPDELGMMPGNSDYLLALVNF
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCEEHHHHH
QALLLGCLSAMPLFYFMLRLLNKPRFALTLWRRFGREMAPQADKKELSLWLLVLVSMVAL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHH
LSYYAKNSNPLLLGGYGLILLLPVMLYGAMRFGYQFNCIIWSSSLLVLFFHYPGYAQRDN
HHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEEEEECCCCCCHHH
LLHNLAVISSMMVVFTLCIYLMSALNTRQRLSHARAQSAALQDPVIGLPNLRALKRDLTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
SLPSTLCFLRVSELDLLSRNYGMQLRIRFKQQLAALLHQVLAPGEGVYHLPGYDLLLRVD
CCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEC
GEDSEEKVLQIYHALEKFRLIWNGLPLHPPLGLGYCSVNADFEHLYLLLGELSSLAEVSL
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHC
TSGKPESVQNGGQWVQEEIKRKVELLHGVQHALDNDGFILMAQPIVGLRGDRYHEILLRM
CCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHH
RDTCGEIISPNDFLPVAHEFGLAYRLDLWVLRNTLKFMDLYRAELPSARFAVNLSPSSLC
HHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHH
RPMLRHDVNSLLEQYQIEPYQLTLEVTESHLLQDTAYAGANLQALREIGCRIALDDFGTG
HHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHCCCCHHHHHHCCCEEEECCCCCC
YASYDRLKMLPVDMLKIDGSFVRDMLTNPVDFQVITSICKVARMKRLAIVAEYVESMEQL
CCCCCHHEECCHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VALKGVGVDYMQGYFIGEPQPLLALLPTPR
HHHHCCCHHHHCCEECCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]