Click here to switch to the map view.

The map label for this gene is ada [H]

Identifier: 157371782

GI number: 157371782

Start: 3918942

End: 3920480

Strand: Reverse

Name: ada [H]

Synonym: Spro_3547

Alternate gene names: 157371782

Gene position: 3920480-3918942 (Counterclockwise)

Preceding gene: 157371786

Following gene: 157371778

Centisome position: 71.95

GC content: 61.34

Gene sequence:

>1539_bases
ATGTCCGAATCCCGCACGTCAGCGCACCAGCCGAGTCGCATAATGAACAAAAAACTGACCCCGAGCCTAGCCATGAAAAA
CCAACAACAGGCGCTGTATCAGGCACTGAGTGCGCGCGATCGTAAATTCGACGGCCGCTTTTTTGTCGGCGTTTCTTCGA
CCGGTATTTATTGCCGCCCGGTGTGCAGTGCCCGCACGCCGAAAATCGAGAACTGTACCTTTTACCCTAGCGCGGCGGCG
GCGGAGCTGGCCGGGTTTCGCCCCTGCCTGAAGTGCCGGCCGGAGTTGGCGCCGGGGCTGGCGTTGATTGATCTGGGCAA
TCGCTATGCCCAGGTGGCGGTACAGTTGATTGAACAGGGCTATCTGTCGGAGCATAGCTGTGAGCAACTGGCCGCGCGGT
TGGGCATTTCCGACCGCCACCTGCGGCGTATCTTTGCCGATCAGTTTGGCGCGTCACCGATTGATTATGCCCAGTCGCAC
CGGCTGTTACAGGCCAAAAGGCTGCTGGCGGATACCGATATGCCGCTAAGTGAGGTGGCGTTTGCCGCCGGTTTCGGCAG
CCTGCGGCGTTTCAACGAGTTGTTCAAGGCACGCTACCGGCTGATCCCCTCGGCGCTGCGCAGCAGCAGCGGCCGTAATG
AACGCACCGTCGCCAGCGGGCTGGTATTTCATCTGGGTTACCGTCCGCCCTATGATTGGCCGCGTATGCTGAGTTTTTTG
CAAACCCGTGCGGTGAGCGGAGTGGAAAAGGTGGAGGGGCAACAGTATCTGCGGGCGATCGCCATCACCCAGGGCGGCAT
TGATTATCACGGCTGGGTCAGCGTGCAGCCGGAAGAAAGCCACAATCGAGTGCGGGTTGAGATTGCCCCCGCCCTCAGCC
GGGTGACCACGGAAGTGCTGCGTCGAATCCGGCAGCTGTTTGATCTGGACGCGGCGCCAGATCTGATCGTCCAGGCCTTG
GGGCAGCTTGCCGCCGATGCGCCGGGCCTGCGTTTGCCCGGCTGCGTCAACGGCTTTGAACAGGCGACCCGGGCGGTGCT
TGGGCAGTTGGTCAGCGTCAAGATGGCGGCGACCTTTGCCGGATGCATGGCTGAACGCTGGGGAACGCCGCTGGAACAGC
CTTACGCCGGCATTACTCATGTGTTCCCCAATGCCGAACAGGTGGCGCGGTTGCAACCCGAGGAACTGCGCCCGCTGGGG
GTGCAGCTGAAACGCGCAGCGGCGCTGATCGCCATCGCACGGGCGGTCACCGAAGGGCGGCTGCAGTTGGAAAACGTGCT
GGATATTGAGCAAGGGATCAAGGCGCTGACCGCGTTGCCGGGGATTGGCAGTTGGACGGCCTGTTACATCGCGATGCGCG
CCTGGTCATGGCCGGATGTGTTTCTGACAGGGGATTATCTGATCAAGCAGCGCTTTCCTGGGATGACGCCACGCCAGATT
GAAAACTATGCCGAATGCTGGCGGCCCTGGCGCTCTTATGCCACGCTGCATCTGTGGCATAACCAGGGTTGGGTACCGTC
AACGGACACTGACGAGTAG

Upstream 100 bases:

>100_bases
TGAAGGGGGGCGACTTTACCCGAGACTTGCCCCTCGGGCAACGTGCCAGCGCATAACTGACGAAAATTTTAGTGACTAAC
GGGAGCGCGTGCTTAACTTA

Downstream 100 bases:

>100_bases
CCGCGTTATTCTTCGGCAGCAATCAGCGCCAGCAGGCTGTCGATCCCTTCACGCATCAGGAAGGTGAGTACTTTCTCGCG
CTCGCTTTCGCTCAGGTGAT

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: Regulatory protein of adaptative response; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MSESRTSAHQPSRIMNKKLTPSLAMKNQQQALYQALSARDRKFDGRFFVGVSSTGIYCRPVCSARTPKIENCTFYPSAAA
AELAGFRPCLKCRPELAPGLALIDLGNRYAQVAVQLIEQGYLSEHSCEQLAARLGISDRHLRRIFADQFGASPIDYAQSH
RLLQAKRLLADTDMPLSEVAFAAGFGSLRRFNELFKARYRLIPSALRSSSGRNERTVASGLVFHLGYRPPYDWPRMLSFL
QTRAVSGVEKVEGQQYLRAIAITQGGIDYHGWVSVQPEESHNRVRVEIAPALSRVTTEVLRRIRQLFDLDAAPDLIVQAL
GQLAADAPGLRLPGCVNGFEQATRAVLGQLVSVKMAATFAGCMAERWGTPLEQPYAGITHVFPNAEQVARLQPEELRPLG
VQLKRAAALIAIARAVTEGRLQLENVLDIEQGIKALTALPGIGSWTACYIAMRAWSWPDVFLTGDYLIKQRFPGMTPRQI
ENYAECWRPWRSYATLHLWHNQGWVPSTDTDE

Sequences:

>Translated_512_residues
MSESRTSAHQPSRIMNKKLTPSLAMKNQQQALYQALSARDRKFDGRFFVGVSSTGIYCRPVCSARTPKIENCTFYPSAAA
AELAGFRPCLKCRPELAPGLALIDLGNRYAQVAVQLIEQGYLSEHSCEQLAARLGISDRHLRRIFADQFGASPIDYAQSH
RLLQAKRLLADTDMPLSEVAFAAGFGSLRRFNELFKARYRLIPSALRSSSGRNERTVASGLVFHLGYRPPYDWPRMLSFL
QTRAVSGVEKVEGQQYLRAIAITQGGIDYHGWVSVQPEESHNRVRVEIAPALSRVTTEVLRRIRQLFDLDAAPDLIVQAL
GQLAADAPGLRLPGCVNGFEQATRAVLGQLVSVKMAATFAGCMAERWGTPLEQPYAGITHVFPNAEQVARLQPEELRPLG
VQLKRAAALIAIARAVTEGRLQLENVLDIEQGIKALTALPGIGSWTACYIAMRAWSWPDVFLTGDYLIKQRFPGMTPRQI
ENYAECWRPWRSYATLHLWHNQGWVPSTDTDE
>Mature_511_residues
SESRTSAHQPSRIMNKKLTPSLAMKNQQQALYQALSARDRKFDGRFFVGVSSTGIYCRPVCSARTPKIENCTFYPSAAAA
ELAGFRPCLKCRPELAPGLALIDLGNRYAQVAVQLIEQGYLSEHSCEQLAARLGISDRHLRRIFADQFGASPIDYAQSHR
LLQAKRLLADTDMPLSEVAFAAGFGSLRRFNELFKARYRLIPSALRSSSGRNERTVASGLVFHLGYRPPYDWPRMLSFLQ
TRAVSGVEKVEGQQYLRAIAITQGGIDYHGWVSVQPEESHNRVRVEIAPALSRVTTEVLRRIRQLFDLDAAPDLIVQALG
QLAADAPGLRLPGCVNGFEQATRAVLGQLVSVKMAATFAGCMAERWGTPLEQPYAGITHVFPNAEQVARLQPEELRPLGV
QLKRAAALIAIARAVTEGRLQLENVLDIEQGIKALTALPGIGSWTACYIAMRAWSWPDVFLTGDYLIKQRFPGMTPRQIE
NYAECWRPWRSYATLHLWHNQGWVPSTDTDE

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=286, Percent_Identity=47.5524475524476, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1788542, Length=155, Percent_Identity=35.4838709677419, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR010316
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 57038; Mature: 56907

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESRTSAHQPSRIMNKKLTPSLAMKNQQQALYQALSARDRKFDGRFFVGVSSTGIYCRP
CCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEE
VCSARTPKIENCTFYPSAAAAELAGFRPCLKCRPELAPGLALIDLGNRYAQVAVQLIEQG
CCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHC
YLSEHSCEQLAARLGISDRHLRRIFADQFGASPIDYAQSHRLLQAKRLLADTDMPLSEVA
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
FAAGFGSLRRFNELFKARYRLIPSALRSSSGRNERTVASGLVFHLGYRPPYDWPRMLSFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCCCCHHHHHHHH
QTRAVSGVEKVEGQQYLRAIAITQGGIDYHGWVSVQPEESHNRVRVEIAPALSRVTTEVL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCEEEEEEHHHHHHHHHHHH
RRIRQLFDLDAAPDLIVQALGQLAADAPGLRLPGCVNGFEQATRAVLGQLVSVKMAATFA
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GCMAERWGTPLEQPYAGITHVFPNAEQVARLQPEELRPLGVQLKRAAALIAIARAVTEGR
HHHHHHCCCCCCCCCCCHHHCCCCHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LQLENVLDIEQGIKALTALPGIGSWTACYIAMRAWSWPDVFLTGDYLIKQRFPGMTPRQI
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEECHHHHHHHCCCCCHHHH
ENYAECWRPWRSYATLHLWHNQGWVPSTDTDE
HHHHHHHHHHHHCEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SESRTSAHQPSRIMNKKLTPSLAMKNQQQALYQALSARDRKFDGRFFVGVSSTGIYCRP
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEE
VCSARTPKIENCTFYPSAAAAELAGFRPCLKCRPELAPGLALIDLGNRYAQVAVQLIEQG
CCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHC
YLSEHSCEQLAARLGISDRHLRRIFADQFGASPIDYAQSHRLLQAKRLLADTDMPLSEVA
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
FAAGFGSLRRFNELFKARYRLIPSALRSSSGRNERTVASGLVFHLGYRPPYDWPRMLSFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCCCCCHHHHHHHH
QTRAVSGVEKVEGQQYLRAIAITQGGIDYHGWVSVQPEESHNRVRVEIAPALSRVTTEVL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCEEEEEEHHHHHHHHHHHH
RRIRQLFDLDAAPDLIVQALGQLAADAPGLRLPGCVNGFEQATRAVLGQLVSVKMAATFA
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GCMAERWGTPLEQPYAGITHVFPNAEQVARLQPEELRPLGVQLKRAAALIAIARAVTEGR
HHHHHHCCCCCCCCCCCHHHCCCCHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LQLENVLDIEQGIKALTALPGIGSWTACYIAMRAWSWPDVFLTGDYLIKQRFPGMTPRQI
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEECHHHHHHHCCCCCHHHH
ENYAECWRPWRSYATLHLWHNQGWVPSTDTDE
HHHHHHHHHHHHCEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]