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The map label for this gene is cysU [H]

Identifier: 157371693

GI number: 157371693

Start: 3827380

End: 3828213

Strand: Reverse

Name: cysU [H]

Synonym: Spro_3456

Alternate gene names: 157371693

Gene position: 3828213-3827380 (Counterclockwise)

Preceding gene: 157371694

Following gene: 157371692

Centisome position: 70.26

GC content: 59.95

Gene sequence:

>834_bases
ATGTTGTCGTTGACCAGCAAGCGGGTTTTACCTGGGTTTGGCCTCAGCCTGGGGAGCAGCCTGTTTTATACCTGCCTGAT
CCTGCTGTTGCCGCTCAGCGCGTTGGTGATGCAGTTGGCGCAGATGAGCCTGGCGCAGTATTGGGAAGTGATTTCCAACC
CGCAGGTGGTGGCGGCTTATAAGGTCACGCTGCTGGCGGCGGGCGTTGCCAGCCTGTTTAACGCGGTGTTCGGCATGCTG
ATGGCCTGGATCCTGACCCGCTATCGTTTTCCCGGTCGTACGCTGCTGGACGGTCTGATCGATCTGCCGTTCGCGCTGCC
GACGGCGGTAGCCGGCCTGACGCTGGCGGGGCTGTTTTCCACCACTGGCTGGTATGGCCAATGGCTGGCGCATTTTGACA
TTAAGGTGACGTTTACCTGGCTCGGCATTGCTGTGGCGATGGCCTTTACCAGCTTGCCGTTCGTGGTACGCACCGTACAG
CCGGTTCTGGAAGAGCTGGGGCCGGAATACGAAGAGGCGGCAGAAACCCTGGGCGCGACTCGCTGGCAGAGTTTTCGTCG
CGTGGTGATCCCTGAGCTGGCACCGGCGCTGTTGGCCGGTACGGCAATCTCTTTTACCCGCAGCCTGGGCGAGTTCGGCG
CGGTGATCTTTATCGCCGGCAACATCGCCTGGAAGACGGAAGTGACCTCGCTGATGATTTTTGTTCGTTTGCAGGAATTT
GATTATCCGGCGGCCAGTGCGATTGCCTCGGTGATCCTGGCGGCCTCGCTGCTGTTGCTGTTCAGCATTAACGTTCTGCA
GAGCCGCTTTGGCCGACGTATCGGGGGGCATTGA

Upstream 100 bases:

>100_bases
TCCGGGTGGAAGATCAGTTTGGTGGCTGGCCGCAGGTGATGAAAACGCATTTCTCCACCGGTGGCGAGTTGGATCAGCTG
TTAGCCGCAGGGCATAAGTA

Downstream 100 bases:

>100_bases
TGGCGGATATCTCTGAGTTCAACGGCGTCGAACGCCCGCGCGTCAACTGGGGCAAGTGGACGCTGATCGGTATCGGCGTG
CTGTTTTCGGTACTGCTGCT

Product: sulfate/thiosulfate transporter subunit

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MLSLTSKRVLPGFGLSLGSSLFYTCLILLLPLSALVMQLAQMSLAQYWEVISNPQVVAAYKVTLLAAGVASLFNAVFGML
MAWILTRYRFPGRTLLDGLIDLPFALPTAVAGLTLAGLFSTTGWYGQWLAHFDIKVTFTWLGIAVAMAFTSLPFVVRTVQ
PVLEELGPEYEEAAETLGATRWQSFRRVVIPELAPALLAGTAISFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFVRLQEF
DYPAASAIASVILAASLLLLFSINVLQSRFGRRIGGH

Sequences:

>Translated_277_residues
MLSLTSKRVLPGFGLSLGSSLFYTCLILLLPLSALVMQLAQMSLAQYWEVISNPQVVAAYKVTLLAAGVASLFNAVFGML
MAWILTRYRFPGRTLLDGLIDLPFALPTAVAGLTLAGLFSTTGWYGQWLAHFDIKVTFTWLGIAVAMAFTSLPFVVRTVQ
PVLEELGPEYEEAAETLGATRWQSFRRVVIPELAPALLAGTAISFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFVRLQEF
DYPAASAIASVILAASLLLLFSINVLQSRFGRRIGGH
>Mature_277_residues
MLSLTSKRVLPGFGLSLGSSLFYTCLILLLPLSALVMQLAQMSLAQYWEVISNPQVVAAYKVTLLAAGVASLFNAVFGML
MAWILTRYRFPGRTLLDGLIDLPFALPTAVAGLTLAGLFSTTGWYGQWLAHFDIKVTFTWLGIAVAMAFTSLPFVVRTVQ
PVLEELGPEYEEAAETLGATRWQSFRRVVIPELAPALLAGTAISFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFVRLQEF
DYPAASAIASVILAASLLLLFSINVLQSRFGRRIGGH

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=277, Percent_Identity=86.6425992779783, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI87082099, Length=259, Percent_Identity=35.1351351351351, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1786980, Length=229, Percent_Identity=33.1877729257642, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1787368, Length=215, Percent_Identity=28.8372093023256, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787369, Length=207, Percent_Identity=28.0193236714976, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787080, Length=246, Percent_Identity=25.2032520325203, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30116; Mature: 30116

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSLTSKRVLPGFGLSLGSSLFYTCLILLLPLSALVMQLAQMSLAQYWEVISNPQVVAAY
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
KVTLLAAGVASLFNAVFGMLMAWILTRYRFPGRTLLDGLIDLPFALPTAVAGLTLAGLFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHH
TTGWYGQWLAHFDIKVTFTWLGIAVAMAFTSLPFVVRTVQPVLEELGPEYEEAAETLGAT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RWQSFRRVVIPELAPALLAGTAISFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFVRLQEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHC
DYPAASAIASVILAASLLLLFSINVLQSRFGRRIGGH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLSLTSKRVLPGFGLSLGSSLFYTCLILLLPLSALVMQLAQMSLAQYWEVISNPQVVAAY
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
KVTLLAAGVASLFNAVFGMLMAWILTRYRFPGRTLLDGLIDLPFALPTAVAGLTLAGLFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHH
TTGWYGQWLAHFDIKVTFTWLGIAVAMAFTSLPFVVRTVQPVLEELGPEYEEAAETLGAT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RWQSFRRVVIPELAPALLAGTAISFTRSLGEFGAVIFIAGNIAWKTEVTSLMIFVRLQEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHHHC
DYPAASAIASVILAASLLLLFSINVLQSRFGRRIGGH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]