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The map label for this gene is cysP [H]

Identifier: 157371694

GI number: 157371694

Start: 3828213

End: 3829235

Strand: Reverse

Name: cysP [H]

Synonym: Spro_3457

Alternate gene names: 157371694

Gene position: 3829235-3828213 (Counterclockwise)

Preceding gene: 157371695

Following gene: 157371693

Centisome position: 70.28

GC content: 56.3

Gene sequence:

>1023_bases
ATGAAACAAACGCGGGTTAAAAATATCGTACTGAAAGGATGGTTGGCGGCGGCGCTGTTAGCCAGCGGCCCGCTGTCGGC
AACCGAATTGCTGAACAGCTCCTATGACGTTTCCCGTGAATTGTTTACCGCGCTGAACCCAGGTTTTGAGCAGCAATGGA
ACCAACAGCATCCGGGCGACAAGCTGACCATCAAACAATCCCACGCCGGCTCCTCCAAGCAGGCGCTGGCGATCCTGCAG
GGGTTGCGCGCCGACGTAGTGACCTACAACCAGGTGACCGACGTGCAGATCCTGCACGATCGCGGCCAACTGATCCCGGC
TGACTGGCAGACCCGTTTACCGAACAACAGCTCTCCTTTCTATTCCACCATGGCTTTCCTGGTGCGTAAGGACAATCCAA
AAGGCATTCATAACTGGAATGATTTGGTGCGTGACGACGTGAAGCTGGTGTTCCCGAACCCGAAAACCTCCGGTAACGGC
CGTTATACCTATCTGGCCGCCTGGGGCGCTGCCAATCAGGCGGATGGCAATGATCAGGCCAAAACCCGCGCCTTTATGAC
CCGTTTCCTGAAAAACGTTCTGGTGTTTGACACCGGCGGCCGCGGCGCGACCACTACCTTCGTCGAGCGCGGCCTGGGCG
ACGTGCTGATCAGTTTTGAGTCGGAAGTGAACAATATTCGCAAGCAGTATGGCGAAGACAAATACGAGGTGATCGTGCCG
CCGGTCGATATTCTGGCGGAATTCCCGGTGGCCTGGGTCGATAAAAACGTGGCAAGAAACGGCACCGAACAGGCGGCCAA
AGACTACCTGAATTATCTCTACAGCCCGGCAGCACAGCAGGTGATCACCAGCTACTACTATCGCGTTTACGACAAACAGG
CGATGGCCGCGGCCAAAGGGCAGTTCCCGGAGACCAAGTTGTTCCGGGTGGAAGATCAGTTTGGTGGCTGGCCGCAGGTG
ATGAAAACGCATTTCTCCACCGGTGGCGAGTTGGATCAGCTGTTAGCCGCAGGGCATAAGTAA

Upstream 100 bases:

>100_bases
AACGGTATATAAAAGCGTTACAGCTCCGCACCTAGTTATAAATACACTGGTCAACATCAGGGCGAAAGCCCGACGGATTG
ACTCAGTGATAAGGTGCACA

Downstream 100 bases:

>100_bases
TGTTGTCGTTGACCAGCAAGCGGGTTTTACCTGGGTTTGGCCTCAGCCTGGGGAGCAGCCTGTTTTATACCTGCCTGATC
CTGCTGTTGCCGCTCAGCGC

Product: thiosulfate transporter subunit

Products: SO42- [Cytoplasm]; phosphate; ADP; S2O32- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKQTRVKNIVLKGWLAAALLASGPLSATELLNSSYDVSRELFTALNPGFEQQWNQQHPGDKLTIKQSHAGSSKQALAILQ
GLRADVVTYNQVTDVQILHDRGQLIPADWQTRLPNNSSPFYSTMAFLVRKDNPKGIHNWNDLVRDDVKLVFPNPKTSGNG
RYTYLAAWGAANQADGNDQAKTRAFMTRFLKNVLVFDTGGRGATTTFVERGLGDVLISFESEVNNIRKQYGEDKYEVIVP
PVDILAEFPVAWVDKNVARNGTEQAAKDYLNYLYSPAAQQVITSYYYRVYDKQAMAAAKGQFPETKLFRVEDQFGGWPQV
MKTHFSTGGELDQLLAAGHK

Sequences:

>Translated_340_residues
MKQTRVKNIVLKGWLAAALLASGPLSATELLNSSYDVSRELFTALNPGFEQQWNQQHPGDKLTIKQSHAGSSKQALAILQ
GLRADVVTYNQVTDVQILHDRGQLIPADWQTRLPNNSSPFYSTMAFLVRKDNPKGIHNWNDLVRDDVKLVFPNPKTSGNG
RYTYLAAWGAANQADGNDQAKTRAFMTRFLKNVLVFDTGGRGATTTFVERGLGDVLISFESEVNNIRKQYGEDKYEVIVP
PVDILAEFPVAWVDKNVARNGTEQAAKDYLNYLYSPAAQQVITSYYYRVYDKQAMAAAKGQFPETKLFRVEDQFGGWPQV
MKTHFSTGGELDQLLAAGHK
>Mature_340_residues
MKQTRVKNIVLKGWLAAALLASGPLSATELLNSSYDVSRELFTALNPGFEQQWNQQHPGDKLTIKQSHAGSSKQALAILQ
GLRADVVTYNQVTDVQILHDRGQLIPADWQTRLPNNSSPFYSTMAFLVRKDNPKGIHNWNDLVRDDVKLVFPNPKTSGNG
RYTYLAAWGAANQADGNDQAKTRAFMTRFLKNVLVFDTGGRGATTTFVERGLGDVLISFESEVNNIRKQYGEDKYEVIVP
PVDILAEFPVAWVDKNVARNGTEQAAKDYLNYLYSPAAQQVITSYYYRVYDKQAMAAAKGQFPETKLFRVEDQFGGWPQV
MKTHFSTGGELDQLLAAGHK

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. This protein specifically binds thiosulfate and is involved in its transmembrane transport [H]

COG id: COG4150

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1788765, Length=317, Percent_Identity=78.2334384858044, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1790351, Length=318, Percent_Identity=47.4842767295598, Blast_Score=306, Evalue=9e-85,

Paralogues:

None

Copy number: 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37970; Mature: 37970

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTRVKNIVLKGWLAAALLASGPLSATELLNSSYDVSRELFTALNPGFEQQWNQQHPGD
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCCCCCC
KLTIKQSHAGSSKQALAILQGLRADVVTYNQVTDVQILHDRGQLIPADWQTRLPNNSSPF
EEEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCHHHCCCCCCCCH
YSTMAFLVRKDNPKGIHNWNDLVRDDVKLVFPNPKTSGNGRYTYLAAWGAANQADGNDQA
HHHHHHHHCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCHHH
KTRAFMTRFLKNVLVFDTGGRGATTTFVERGLGDVLISFESEVNNIRKQYGEDKYEVIVP
HHHHHHHHHHHHHEEEECCCCCCHHHHHHHCHHHEEEHHHHHHHHHHHHHCCCCEEEEEC
PVDILAEFPVAWVDKNVARNGTEQAAKDYLNYLYSPAAQQVITSYYYRVYDKQAMAAAKG
CHHHHHHCCHHHHCCHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCC
QFPETKLFRVEDQFGGWPQVMKTHFSTGGELDQLLAAGHK
CCCCCEEEEECHHCCCCHHHHHHHHCCCCCHHHHHHCCCC
>Mature Secondary Structure
MKQTRVKNIVLKGWLAAALLASGPLSATELLNSSYDVSRELFTALNPGFEQQWNQQHPGD
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCCCCCC
KLTIKQSHAGSSKQALAILQGLRADVVTYNQVTDVQILHDRGQLIPADWQTRLPNNSSPF
EEEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCHHHCCCCCCCCH
YSTMAFLVRKDNPKGIHNWNDLVRDDVKLVFPNPKTSGNGRYTYLAAWGAANQADGNDQA
HHHHHHHHCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCHHH
KTRAFMTRFLKNVLVFDTGGRGATTTFVERGLGDVLISFESEVNNIRKQYGEDKYEVIVP
HHHHHHHHHHHHHEEEECCCCCCHHHHHHHCHHHEEEHHHHHHHHHHHHHCCCCEEEEEC
PVDILAEFPVAWVDKNVARNGTEQAAKDYLNYLYSPAAQQVITSYYYRVYDKQAMAAAKG
CHHHHHHCCHHHHCCHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCC
QFPETKLFRVEDQFGGWPQVMKTHFSTGGELDQLLAAGHK
CCCCCEEEEECHHCCCCHHHHHHHHCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: SO42- [Periplasm]; H2O; ATP; S2O32- [Periplasm] [C]

Specific reaction: SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2188959; 9205837; 9278503; 9298646 [H]