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The map label for this gene is entE [H]

Identifier: 157371657

GI number: 157371657

Start: 3784139

End: 3785767

Strand: Reverse

Name: entE [H]

Synonym: Spro_3420

Alternate gene names: 157371657

Gene position: 3785767-3784139 (Counterclockwise)

Preceding gene: 157371658

Following gene: 157371656

Centisome position: 69.48

GC content: 58.69

Gene sequence:

>1629_bases
ATGACCATTGCTTTTACCCCCTGGCCTGAGGCGTTAGCCCATCGCTATCGCGAACGCGGCTATTGGACCGACCGGCCCCT
GACCGACATCATCACTCGCCAGGCCAAAAATGACGCTATCGCGCTGATCGACCCGGAGCGCAGCCTGAGTTACCGCCGGC
TTAATCAGCTCTCCGATCGGTTGGCTGCCGCCCTGCAGCGCCGCGGCATTCAGAGCGGAGATACTGCTCTGGTGCAGCTG
GGCAACGTGGTCGAGTTCTATGTAACCTTCTTCGCGTTGTTGAAAATTGGCGTGGTACCGGTGAACGCGTTGTTCAGCCA
TCAACGCAACGAGCTGAACGCCTACGCCGTACAGATCAAACCAGCATTGCTGATTGCCGACCGTCAGCATGGCCTGTTTG
GCAACGACGAATTTCTGACGGCGTTTCGCGCCGAGCATCCTTCATTGCGCGTGGTGGCATTGCGCAATCAGGACGGGGGC
GAGCAGTCGCTGGCGGCCTGGCTGGAAGAGGACAGCAGTGGTTTTGTTGCGACGCCAAGCCCGGCCGACCAGGTGGCGTT
TTTCCAGCTTTCCGGTGGCAGCACCGGAACGCCAAAGCTGATCCCGCGGACTCACAACGATTACTACTACAGCATTCGCC
GCAGCGTGGAAATTTGCCATTTCGACGTCGATACCCGCTACCTGTGCGCGCTGCCGGTGGCCCATAACTATCCGATGAGT
TCTCCGGGCGTCCTGGGCGTGTTTTATGGCGCCGGGCTGGTGGTGTTTGCCAGCGACCCGGATGCCGGACAATGTTTCCG
TTTGATCGAGCAGCATCAGATTAACGTGACGGCGCTGGTGCCACCGGCGGTGACGCTGTGGCTGCAGGCGATTGAAGAGT
GGGGCGGTTGCCAGCAACTGACCAGCCTTAAGCTGTTGCAGGTGGGCGGCGCCAAGCTGGGCGAAACCCTGGCGGCACGT
ATTCCGGCCGAGATCGGCTGCCAGTTGCAGCAGGTGTTCGGCATGGCGGAAGGCCTGGTGAACTACACCCGTCTGGACGA
TGACGATCAGCATATTCTGACCACTCAGGGCTGCCCGATGTCGCCGGATGACGAGCTGTGGGTGGCCGATGAGGACGGCA
ACCCGCTGCCGGTGGGGGAAACCGGGCGTCTGATGACCCGTGGCCCTTATACCTTCCGCGGTTATTACCAAAGTCCGGAG
CACAACGCCGCCGCCTTTGATAAGGACGGTTTCTACTGTTCCGGCGATTTGATCAGCCTGACCGAAGATGGCTATGTGAA
AGTTGAAGGGCGACAAAAAGATCAGATTAACCGTGGTGGGGAAAAGATCGCTGCGGAAGAAATCGAAAACCTGTTATTGC
GCCATCCGGAAGTGATCAATGCGGCGTTGGTGTCGATGCCGGACGAACTGATGGGTGAAAAAAGCTGCGCCTATATCATC
GCGACCAGCGCGTTGAAGCCGGTAGTGTTGCGCCGCCATTTACGCGGCGAGGGCGTGGCCGAATTTAAATTACCCGACCG
TTTTATACAGGTTGATACGCTGCCGCTCACCCCGGTTGGCAAAGTGGATAAAAAACTGTTGCGCCAACGCCTGGAGGCGC
AACAACTGACTCTGGTCCAGGGAGAATAA

Upstream 100 bases:

>100_bases
TTGTGCAAGACTCTTCGCCGGAATCCGAATGGCATGAAACCGGCACCAAACTCAGTACCATTTCGCGGGCGTTTGGCCTG
AACCAAGGATAGACATTCTG

Downstream 100 bases:

>100_bases
GTCATGGCCATTCCAAAACTGAATGATTACGCGTTGCCGACTCAGGCTGAACTGCCGACCAACAAAGTGAGCTGGACGGT
GGAACCGCAGCGCGCTGCAC

Product: enterobactin synthase subunit E

Products: NA

Alternate protein names: Enterochelin synthase E; 2,3-dihydroxybenzoate-AMP ligase; Dihydroxybenzoic acid-activating enzyme; S-dihydroxybenzoyltransferase [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MTIAFTPWPEALAHRYRERGYWTDRPLTDIITRQAKNDAIALIDPERSLSYRRLNQLSDRLAAALQRRGIQSGDTALVQL
GNVVEFYVTFFALLKIGVVPVNALFSHQRNELNAYAVQIKPALLIADRQHGLFGNDEFLTAFRAEHPSLRVVALRNQDGG
EQSLAAWLEEDSSGFVATPSPADQVAFFQLSGGSTGTPKLIPRTHNDYYYSIRRSVEICHFDVDTRYLCALPVAHNYPMS
SPGVLGVFYGAGLVVFASDPDAGQCFRLIEQHQINVTALVPPAVTLWLQAIEEWGGCQQLTSLKLLQVGGAKLGETLAAR
IPAEIGCQLQQVFGMAEGLVNYTRLDDDDQHILTTQGCPMSPDDELWVADEDGNPLPVGETGRLMTRGPYTFRGYYQSPE
HNAAAFDKDGFYCSGDLISLTEDGYVKVEGRQKDQINRGGEKIAAEEIENLLLRHPEVINAALVSMPDELMGEKSCAYII
ATSALKPVVLRRHLRGEGVAEFKLPDRFIQVDTLPLTPVGKVDKKLLRQRLEAQQLTLVQGE

Sequences:

>Translated_542_residues
MTIAFTPWPEALAHRYRERGYWTDRPLTDIITRQAKNDAIALIDPERSLSYRRLNQLSDRLAAALQRRGIQSGDTALVQL
GNVVEFYVTFFALLKIGVVPVNALFSHQRNELNAYAVQIKPALLIADRQHGLFGNDEFLTAFRAEHPSLRVVALRNQDGG
EQSLAAWLEEDSSGFVATPSPADQVAFFQLSGGSTGTPKLIPRTHNDYYYSIRRSVEICHFDVDTRYLCALPVAHNYPMS
SPGVLGVFYGAGLVVFASDPDAGQCFRLIEQHQINVTALVPPAVTLWLQAIEEWGGCQQLTSLKLLQVGGAKLGETLAAR
IPAEIGCQLQQVFGMAEGLVNYTRLDDDDQHILTTQGCPMSPDDELWVADEDGNPLPVGETGRLMTRGPYTFRGYYQSPE
HNAAAFDKDGFYCSGDLISLTEDGYVKVEGRQKDQINRGGEKIAAEEIENLLLRHPEVINAALVSMPDELMGEKSCAYII
ATSALKPVVLRRHLRGEGVAEFKLPDRFIQVDTLPLTPVGKVDKKLLRQRLEAQQLTLVQGE
>Mature_541_residues
TIAFTPWPEALAHRYRERGYWTDRPLTDIITRQAKNDAIALIDPERSLSYRRLNQLSDRLAAALQRRGIQSGDTALVQLG
NVVEFYVTFFALLKIGVVPVNALFSHQRNELNAYAVQIKPALLIADRQHGLFGNDEFLTAFRAEHPSLRVVALRNQDGGE
QSLAAWLEEDSSGFVATPSPADQVAFFQLSGGSTGTPKLIPRTHNDYYYSIRRSVEICHFDVDTRYLCALPVAHNYPMSS
PGVLGVFYGAGLVVFASDPDAGQCFRLIEQHQINVTALVPPAVTLWLQAIEEWGGCQQLTSLKLLQVGGAKLGETLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDDDQHILTTQGCPMSPDDELWVADEDGNPLPVGETGRLMTRGPYTFRGYYQSPEH
NAAAFDKDGFYCSGDLISLTEDGYVKVEGRQKDQINRGGEKIAAEEIENLLLRHPEVINAALVSMPDELMGEKSCAYIIA
TSALKPVVLRRHLRGEGVAEFKLPDRFIQVDTLPLTPVGKVDKKLLRQRLEAQQLTLVQGE

Specific function: Activates the carboxylate group of 2,3-dihydroxy- benzoate (2,3-DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate. Then, catalyzes the acylation of holo-EntB with 2,3-DHB adenylate, preparing that molecule for amide bond formation with

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. EntE subfamily [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=514, Percent_Identity=25.6809338521401, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI122937307, Length=521, Percent_Identity=25.7197696737044, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI28416953, Length=534, Percent_Identity=25.2808988764045, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI156151445, Length=534, Percent_Identity=21.3483146067416, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI58082049, Length=524, Percent_Identity=24.0458015267176, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI187761345, Length=559, Percent_Identity=24.1502683363148, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI187761343, Length=559, Percent_Identity=24.1502683363148, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI157311624, Length=529, Percent_Identity=24.3856332703214, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI157311622, Length=529, Percent_Identity=24.3856332703214, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI42544132, Length=556, Percent_Identity=21.4028776978417, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI115511026, Length=532, Percent_Identity=23.8721804511278, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI8923896, Length=549, Percent_Identity=21.4936247723133, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI253970489, Length=378, Percent_Identity=23.5449735449735, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI40807491, Length=283, Percent_Identity=25.4416961130742, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI57165410, Length=273, Percent_Identity=26.007326007326, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI57165412, Length=273, Percent_Identity=26.007326007326, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1786810, Length=534, Percent_Identity=71.3483146067416, Blast_Score=788, Evalue=0.0,
Organism=Escherichia coli, GI145693145, Length=534, Percent_Identity=30.7116104868914, Blast_Score=208, Evalue=8e-55,
Organism=Escherichia coli, GI1788107, Length=535, Percent_Identity=25.0467289719626, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI221142682, Length=492, Percent_Identity=25, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1786529, Length=563, Percent_Identity=22.3801065719361, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1790505, Length=530, Percent_Identity=23.0188679245283, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786801, Length=455, Percent_Identity=22.6373626373626, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17557194, Length=534, Percent_Identity=26.9662921348315, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17538037, Length=540, Percent_Identity=25.9259259259259, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17560308, Length=531, Percent_Identity=24.6704331450094, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI32563687, Length=515, Percent_Identity=23.3009708737864, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17558820, Length=511, Percent_Identity=23.2876712328767, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71994694, Length=521, Percent_Identity=24.1842610364683, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71994703, Length=521, Percent_Identity=24.1842610364683, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71994690, Length=521, Percent_Identity=24.1842610364683, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17559526, Length=527, Percent_Identity=23.1499051233397, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71996755, Length=495, Percent_Identity=23.8383838383838, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17531443, Length=322, Percent_Identity=23.2919254658385, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71985884, Length=386, Percent_Identity=24.8704663212435, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71983001, Length=547, Percent_Identity=22.4862888482633, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71982997, Length=547, Percent_Identity=22.3034734917733, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=550, Percent_Identity=21.6363636363636, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6319264, Length=551, Percent_Identity=22.5045372050817, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323182, Length=547, Percent_Identity=22.8519195612431, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24581924, Length=537, Percent_Identity=24.7672253258845, Blast_Score=142, Evalue=8e-34,
Organism=Drosophila melanogaster, GI19922652, Length=540, Percent_Identity=23.7037037037037, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24656500, Length=529, Percent_Identity=24.3856332703214, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21355181, Length=569, Percent_Identity=23.5500878734622, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI18859661, Length=372, Percent_Identity=27.1505376344086, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24667955, Length=582, Percent_Identity=23.7113402061856, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI62472339, Length=558, Percent_Identity=23.6559139784946, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356947, Length=430, Percent_Identity=23.7209302325581, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24667959, Length=381, Percent_Identity=25.1968503937008, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI20130357, Length=363, Percent_Identity=25.068870523416, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21356441, Length=481, Percent_Identity=21.2058212058212, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24648260, Length=281, Percent_Identity=23.4875444839858, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648255, Length=481, Percent_Identity=20.5821205821206, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24648253, Length=482, Percent_Identity=20.5394190871369, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =2.7.7.58 [H]

Molecular weight: Translated: 60065; Mature: 59934

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAFTPWPEALAHRYRERGYWTDRPLTDIITRQAKNDAIALIDPERSLSYRRLNQLSDR
CEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHH
LAAALQRRGIQSGDTALVQLGNVVEFYVTFFALLKIGVVPVNALFSHQRNELNAYAVQIK
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEEE
PALLIADRQHGLFGNDEFLTAFRAEHPSLRVVALRNQDGGEQSLAAWLEEDSSGFVATPS
EEEEEEECCCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCEEECCC
PADQVAFFQLSGGSTGTPKLIPRTHNDYYYSIRRSVEICHFDVDTRYLCALPVAHNYPMS
CCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCEEEEEEECCCCEEEEECCCCCCCCC
SPGVLGVFYGAGLVVFASDPDAGQCFRLIEQHQINVTALVPPAVTLWLQAIEEWGGCQQL
CCCEEEEEECCCEEEEECCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCHHHH
TSLKLLQVGGAKLGETLAARIPAEIGCQLQQVFGMAEGLVNYTRLDDDDQHILTTQGCPM
HHHHHHHHCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
SPDDELWVADEDGNPLPVGETGRLMTRGPYTFRGYYQSPEHNAAAFDKDGFYCSGDLISL
CCCCCEEEECCCCCCCCCCCCCCEEECCCCEEECEECCCCCCCCEECCCCCEECCCEEEE
TEDGYVKVEGRQKDQINRGGEKIAAEEIENLLLRHPEVINAALVSMPDELMGEKSCAYII
CCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHCCCCEEEEE
ATSALKPVVLRRHLRGEGVAEFKLPDRFIQVDTLPLTPVGKVDKKLLRQRLEAQQLTLVQ
EHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEE
GE
CC
>Mature Secondary Structure 
TIAFTPWPEALAHRYRERGYWTDRPLTDIITRQAKNDAIALIDPERSLSYRRLNQLSDR
EEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHH
LAAALQRRGIQSGDTALVQLGNVVEFYVTFFALLKIGVVPVNALFSHQRNELNAYAVQIK
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEEE
PALLIADRQHGLFGNDEFLTAFRAEHPSLRVVALRNQDGGEQSLAAWLEEDSSGFVATPS
EEEEEEECCCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCEEECCC
PADQVAFFQLSGGSTGTPKLIPRTHNDYYYSIRRSVEICHFDVDTRYLCALPVAHNYPMS
CCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCEEEEEEECCCCEEEEECCCCCCCCC
SPGVLGVFYGAGLVVFASDPDAGQCFRLIEQHQINVTALVPPAVTLWLQAIEEWGGCQQL
CCCEEEEEECCCEEEEECCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCHHHH
TSLKLLQVGGAKLGETLAARIPAEIGCQLQQVFGMAEGLVNYTRLDDDDQHILTTQGCPM
HHHHHHHHCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
SPDDELWVADEDGNPLPVGETGRLMTRGPYTFRGYYQSPEHNAAAFDKDGFYCSGDLISL
CCCCCEEEECCCCCCCCCCCCCCEEECCCCEEECEECCCCCCCCEECCCCCEECCCEEEE
TEDGYVKVEGRQKDQINRGGEKIAAEEIENLLLRHPEVINAALVSMPDELMGEKSCAYII
CCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHCCCCEEEEE
ATSALKPVVLRRHLRGEGVAEFKLPDRFIQVDTLPLTPVGKVDKKLLRQRLEAQQLTLVQ
EHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEE
GE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]