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The map label for this gene is entB [H]

Identifier: 157371656

GI number: 157371656

Start: 3783281

End: 3784135

Strand: Reverse

Name: entB [H]

Synonym: Spro_3419

Alternate gene names: 157371656

Gene position: 3784135-3783281 (Counterclockwise)

Preceding gene: 157371657

Following gene: 157371655

Centisome position: 69.45

GC content: 55.79

Gene sequence:

>855_bases
ATGGCCATTCCAAAACTGAATGATTACGCGTTGCCGACTCAGGCTGAACTGCCGACCAACAAAGTGAGCTGGACGGTGGA
ACCGCAGCGCGCTGCACTGTTGATCCATGATATGCAGCAGTATTTCCTGAATTTCTGGGGTGAAGACAGCCCGTTGATCA
AGCAGGTGGTGGAGAATATTGCCAACCTGCGTCGTTACTGCAAATCACAGGGGATCCCGGTGTTTTACACCGCGCAGCCT
AATCAGCAGAGCGATGAAGACCGTGCATTACTCAATGACATGTGGGGGCCGGGCCTGAATAAGCACCCGGAACAGCAGGC
GGTGGTCGCCGCTCTGGCCCCGGAAGAAGACGATACGGTGTTAGTGAAGTGGCGCTACAGTGCCTTCCACCGCTCACCGC
TGCAGGAAATTCTGCAGGAGTCGGGGCGCGATCAGTTGATCATCTGTGGTGTCTACGCCCATATCGGCTGTATGACTACG
GCGATCGACGCCTTCATGCGCAATATTCAGCCGTTTATGGTGGCTGACGGCCTGGCCGATTTCTCCCGCGAAGAGCATCT
GATGGCGCTGCGTTATACCGCCGGCCGTTGTGGCCGCGTGGTGACCACTGCCGAACTGTTGCCGGGCATTGCCAGCAAGG
CTGCCTTACGCCAGCAAATCATGCCTTTTCTCGATGAAGACAGTGAAGATATGGGCGACGATGAAAACCTGATCGACTAC
GGTCTGGATTCAGTGCGCATCATGGAGTTGGCGGCACGTTGGCGTAAAATTCGTGGCGATATTGATTTTATCGCACTGGC
CAAGAACCCGACCATCGATGGCTGGTGGACGCTGCTGTCGGAGGGAAAAGCCTGA

Upstream 100 bases:

>100_bases
TGATACGCTGCCGCTCACCCCGGTTGGCAAAGTGGATAAAAAACTGTTGCGCCAACGCCTGGAGGCGCAACAACTGACTC
TGGTCCAGGGAGAATAAGTC

Downstream 100 bases:

>100_bases
TGGAACAACGGGGCGATTTCGCCGGTAAGCGAGTCTGGGTCACTGGTGCTGGGGCCGGGATTGGTCTGGCAACGGCATTG
GCGTTTGTGCAGGCCGGTGC

Product: isochorismatase

Products: NA

Alternate protein names: 2,3 dihydro-2,3 dihydroxybenzoate synthase; Enterobactin synthase component B; Enterochelin synthase B; Isochorismate lyase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MAIPKLNDYALPTQAELPTNKVSWTVEPQRAALLIHDMQQYFLNFWGEDSPLIKQVVENIANLRRYCKSQGIPVFYTAQP
NQQSDEDRALLNDMWGPGLNKHPEQQAVVAALAPEEDDTVLVKWRYSAFHRSPLQEILQESGRDQLIICGVYAHIGCMTT
AIDAFMRNIQPFMVADGLADFSREEHLMALRYTAGRCGRVVTTAELLPGIASKAALRQQIMPFLDEDSEDMGDDENLIDY
GLDSVRIMELAARWRKIRGDIDFIALAKNPTIDGWWTLLSEGKA

Sequences:

>Translated_284_residues
MAIPKLNDYALPTQAELPTNKVSWTVEPQRAALLIHDMQQYFLNFWGEDSPLIKQVVENIANLRRYCKSQGIPVFYTAQP
NQQSDEDRALLNDMWGPGLNKHPEQQAVVAALAPEEDDTVLVKWRYSAFHRSPLQEILQESGRDQLIICGVYAHIGCMTT
AIDAFMRNIQPFMVADGLADFSREEHLMALRYTAGRCGRVVTTAELLPGIASKAALRQQIMPFLDEDSEDMGDDENLIDY
GLDSVRIMELAARWRKIRGDIDFIALAKNPTIDGWWTLLSEGKA
>Mature_283_residues
AIPKLNDYALPTQAELPTNKVSWTVEPQRAALLIHDMQQYFLNFWGEDSPLIKQVVENIANLRRYCKSQGIPVFYTAQPN
QQSDEDRALLNDMWGPGLNKHPEQQAVVAALAPEEDDTVLVKWRYSAFHRSPLQEILQESGRDQLIICGVYAHIGCMTTA
IDAFMRNIQPFMVADGLADFSREEHLMALRYTAGRCGRVVTTAELLPGIASKAALRQQIMPFLDEDSEDMGDDENLIDYG
LDSVRIMELAARWRKIRGDIDFIALAKNPTIDGWWTLLSEGKA

Specific function: Required for production of 2,3-DHB. Also serves as an aryl carrier protein and plays a role in enterobactin assembly [H]

COG id: COG1535

COG function: function code Q; Isochorismate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Escherichia coli, GI1786811, Length=282, Percent_Identity=72.6950354609929, Blast_Score=433, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009081
- InterPro:   IPR016291
- InterPro:   IPR000868
- InterPro:   IPR006163 [H]

Pfam domain/function: PF00857 Isochorismatase; PF00550 PP-binding [H]

EC number: =3.3.2.1 [H]

Molecular weight: Translated: 32097; Mature: 31966

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50075 ACP_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPKLNDYALPTQAELPTNKVSWTVEPQRAALLIHDMQQYFLNFWGEDSPLIKQVVENI
CCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ANLRRYCKSQGIPVFYTAQPNQQSDEDRALLNDMWGPGLNKHPEQQAVVAALAPEEDDTV
HHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHHEEEEECCCCCCEE
LVKWRYSAFHRSPLQEILQESGRDQLIICGVYAHIGCMTTAIDAFMRNIQPFMVADGLAD
EEEEEHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
FSREEHLMALRYTAGRCGRVVTTAELLPGIASKAALRQQIMPFLDEDSEDMGDDENLIDY
HCHHHHEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
GLDSVRIMELAARWRKIRGDIDFIALAKNPTIDGWWTLLSEGKA
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCCC
>Mature Secondary Structure 
AIPKLNDYALPTQAELPTNKVSWTVEPQRAALLIHDMQQYFLNFWGEDSPLIKQVVENI
CCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ANLRRYCKSQGIPVFYTAQPNQQSDEDRALLNDMWGPGLNKHPEQQAVVAALAPEEDDTV
HHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHHEEEEECCCCCCEE
LVKWRYSAFHRSPLQEILQESGRDQLIICGVYAHIGCMTTAIDAFMRNIQPFMVADGLAD
EEEEEHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
FSREEHLMALRYTAGRCGRVVTTAELLPGIASKAALRQQIMPFLDEDSEDMGDDENLIDY
HCHHHHEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
GLDSVRIMELAARWRKIRGDIDFIALAKNPTIDGWWTLLSEGKA
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]