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The map label for this gene is mlaA [H]

Identifier: 157371619

GI number: 157371619

Start: 3743556

End: 3744314

Strand: Reverse

Name: mlaA [H]

Synonym: Spro_3382

Alternate gene names: 157371619

Gene position: 3744314-3743556 (Counterclockwise)

Preceding gene: 157371620

Following gene: 157371617

Centisome position: 68.72

GC content: 56.79

Gene sequence:

>759_bases
ATGAACTTTCGCCTGACTGGGCTGGCTTTCACAACGGTGTTATTGGTGGGCTGTGCCAGCACGCCACAAGATGAACCACA
AGGGCGATCCGATCCTCTGGAAGGATTTAACCGGACGATGTTCAACTTTAACTATAACGTCCTGGATCCGTATGTCCTGC
GCCCGGTGGCCGTGGCCTGGCGTGACTACCTGCCGATGCCGGCGCGTAATGGCATCAGCAACTTCACCTCCAACCTGGAA
GAGCCGGCCAGCATGGTCAACTCCTTCCTGAAAGGTGATCCGTACAAGGGGATGATTCACTTTAACCGTTTCTTCCTCAA
CACCCTGTTGGGGATGGGCGGCCTGATCGACGTGGCTGGCATGGCTAACCCGAAACTGGCACGCGAAGAGCCGAACCGTT
TCGGCAGCACTCTGGGGCACTACGGTGTCGGCTATGGTCCTTATGTGATGTTGCCGGGCTATGGCAGTTTCACCCTGCGT
GAAGAGGGCGGTGACTGGGCAGATGACCTTTATCCGGTGCTGAGCTACCTGACCTTCTGGATGTCGGCAGGCAAGTGGGT
GGTTGAGGGGATTGAAACCCGTGCCGAGCTGCTCGATTCCGACGGCCTGTTGCGTAACTCTTCCGATCCATACCTGATGG
TGCGTGAGGCCTACTTCCAGCGCAACGATTTCCTGGCGAACGGTGGTTCACTGAAACCGGAAGTTAACCCGAACGCCCAG
GCGATCCAGGGTGATTTGGACGAGATCGACTCGCAATAA

Upstream 100 bases:

>100_bases
GTGCGCTGCAGAATTTCAGCGGTAAAGAGCAGATTGACGTTCAGATAAACAAACAGGTCCCCTGAATAAAAAGACCGAAC
GTGTTTTCATGGAGAAGAAT

Downstream 100 bases:

>100_bases
CCTCGGTTAGCGAACAGAAACAACCCGTCGACTGGCGGGTTTTTTTTCGCCCGGCAAATAGTAGGCAATAAAAAAGCGCC
CCGCAGGGCGCTTTGGCTTC

Product: VacJ family lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MNFRLTGLAFTTVLLVGCASTPQDEPQGRSDPLEGFNRTMFNFNYNVLDPYVLRPVAVAWRDYLPMPARNGISNFTSNLE
EPASMVNSFLKGDPYKGMIHFNRFFLNTLLGMGGLIDVAGMANPKLAREEPNRFGSTLGHYGVGYGPYVMLPGYGSFTLR
EEGGDWADDLYPVLSYLTFWMSAGKWVVEGIETRAELLDSDGLLRNSSDPYLMVREAYFQRNDFLANGGSLKPEVNPNAQ
AIQGDLDEIDSQ

Sequences:

>Translated_252_residues
MNFRLTGLAFTTVLLVGCASTPQDEPQGRSDPLEGFNRTMFNFNYNVLDPYVLRPVAVAWRDYLPMPARNGISNFTSNLE
EPASMVNSFLKGDPYKGMIHFNRFFLNTLLGMGGLIDVAGMANPKLAREEPNRFGSTLGHYGVGYGPYVMLPGYGSFTLR
EEGGDWADDLYPVLSYLTFWMSAGKWVVEGIETRAELLDSDGLLRNSSDPYLMVREAYFQRNDFLANGGSLKPEVNPNAQ
AIQGDLDEIDSQ
>Mature_252_residues
MNFRLTGLAFTTVLLVGCASTPQDEPQGRSDPLEGFNRTMFNFNYNVLDPYVLRPVAVAWRDYLPMPARNGISNFTSNLE
EPASMVNSFLKGDPYKGMIHFNRFFLNTLLGMGGLIDVAGMANPKLAREEPNRFGSTLGHYGVGYGPYVMLPGYGSFTLR
EEGGDWADDLYPVLSYLTFWMSAGKWVVEGIETRAELLDSDGLLRNSSDPYLMVREAYFQRNDFLANGGSLKPEVNPNAQ
AIQGDLDEIDSQ

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=252, Percent_Identity=77.7777777777778, Blast_Score=410, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 28055; Mature: 28055

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFRLTGLAFTTVLLVGCASTPQDEPQGRSDPLEGFNRTMFNFNYNVLDPYVLRPVAVAW
CCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHEEEECCCCCCCHHHHHHHHHHH
RDYLPMPARNGISNFTSNLEEPASMVNSFLKGDPYKGMIHFNRFFLNTLLGMGGLIDVAG
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEECCC
MANPKLAREEPNRFGSTLGHYGVGYGPYVMLPGYGSFTLREEGGDWADDLYPVLSYLTFW
CCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHH
MSAGKWVVEGIETRAELLDSDGLLRNSSDPYLMVREAYFQRNDFLANGGSLKPEVNPNAQ
HHCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHCCEECCCCCCCCCCCCCHH
AIQGDLDEIDSQ
HHCCCHHHCCCC
>Mature Secondary Structure
MNFRLTGLAFTTVLLVGCASTPQDEPQGRSDPLEGFNRTMFNFNYNVLDPYVLRPVAVAW
CCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHEEEECCCCCCCHHHHHHHHHHH
RDYLPMPARNGISNFTSNLEEPASMVNSFLKGDPYKGMIHFNRFFLNTLLGMGGLIDVAG
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEECCC
MANPKLAREEPNRFGSTLGHYGVGYGPYVMLPGYGSFTLREEGGDWADDLYPVLSYLTFW
CCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHH
MSAGKWVVEGIETRAELLDSDGLLRNSSDPYLMVREAYFQRNDFLANGGSLKPEVNPNAQ
HHCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHCCEECCCCCCCCCCCCCHH
AIQGDLDEIDSQ
HHCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]