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The map label for this gene is nuoF [H]

Identifier: 157371541

GI number: 157371541

Start: 3658571

End: 3659923

Strand: Reverse

Name: nuoF [H]

Synonym: Spro_3304

Alternate gene names: 157371541

Gene position: 3659923-3658571 (Counterclockwise)

Preceding gene: 157371542

Following gene: 157371540

Centisome position: 67.17

GC content: 58.46

Gene sequence:

>1353_bases
ATGATTAAAGACATTGTACGCACTCCCGAAATGCACCCGCTGACCTGGCGGCTGCGCGATGACAAACAGCCGGTCTGGCT
GGATGAATATCGCAGCAAGAACGGCTATGCCGGTGCTGAAAAGGCGATTAAAGGCCTGGCTCCGGACGAGATCGTCACCC
TGGTGAAAGACGCCGGGCTGAAAGGTCGCGGCGGTGCAGGTTTCTCCACCGGTCTGAAGTGGAGCCTGATGCCGAAAGAC
GAATCCATGAACATCCGTTACCTGCTGTGTAACGCCGATGAAATGGAGCCGGGCACCTATAAAGACCGCTTGCTGATGGA
GCAACTGCCGCACCTGTTGGTGGAAGGCATGCTGATTTCGGCGTTCGCGCTGAAGGCTTATCGTGGCTACATCTTCCTGC
GCGGTGAGTACATCGAAGCTGCGGTGCATTTGCGCCGTGCGATCGCCGAAGCCACCGAAGCCGGTTATTTGGGCAAGAAC
ATCATGGGCAGCGGCTTCGACTTCGAGCTGATTGTTCATACCGGTGCCGGTCGTTACATCTGTGGCGAAGAAACCGCCCT
GATCAACTCGCTGGAAGGCCGCCGTGCCAACCCGCGTTCCAAGCCACCTTTCCCGGCTTCCTCCGGCGTTTGGGGCAAAC
CGACCTGCGTCAATAACGTAGAAACCCTGTGTAACGTTCCGGCGATCCTGGCGAACGGCGTTGAGTGGTACAAGGGGATC
TCCGCCGGTAAGAGTAACGATGCCGGTACCAAACTGATGGGCTTCTCCGGCCGGGTGAAAAACCCAGGCGTTTGGGAGCT
GCCGTTTGGTACCACCGCACGTGAAATTCTGGAAGATTACGCCGGTGGCATGCGTGACGGTCTGCGTTTCAAAGCCTGGC
AGCCAGGCGGCGCGGGCACCGACTTCCTGACCGCCGATCACCTCGATCTGCCGATGGACTTCGAGAGCATCGGTAAAGCC
GGCAGCCGTCTCGGTACTGCACTGGCGATGGCAGTAGACCACGAGATTGGCATGGTTCCGCTGGTGCGCAACCTGGAAGA
GTTTTTCTCTCGCGAGTCCTGCGGCTGGTGTACGCCATGCCGTGACGGTCTGCCGTGGAGCGTGAAGATCCTGCGTGCGC
TGGAAAACGGCGAAGGGCAGCCGGGGGATATCGAAACCCTTGAGCAGCTGTGCCGTTCTCTCGGCCCGGGCAAAACCTTC
TGCGCCCACGCGCCAGGTGCCGTAGAGCCACTGCAAAGCGCAATTAAATATTTCCGTGAAGAGTTCGAAGCGGGCATCAA
AGTGAAGCACTTCAGCAACGCGCATGCGATTGGCGGTATTCAGGCCAATAATCTGCTGAAGCAACGCTGGTAA

Upstream 100 bases:

>100_bases
GCCTGGGCAACTGCGATAAAGGGCCAACCATGATGATTGATGAGGACACTCACAGTCAGCTGAAGCCTGAAGATATCGAG
ACGCTACTGGAGCAGTATCA

Downstream 100 bases:

>100_bases
GCCGACTCGCCCCGACGACACGGCCAGAATTTTTGATTAACGCCTGCCGCAAGGCAGGCCACTTGGAAGCATGCTGACTA
TGGCTACGATTCATGTAGAC

Product: NADH dehydrogenase I subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F; NDH-1 subunit F; NUO6 [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MIKDIVRTPEMHPLTWRLRDDKQPVWLDEYRSKNGYAGAEKAIKGLAPDEIVTLVKDAGLKGRGGAGFSTGLKWSLMPKD
ESMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVHLRRAIAEATEAGYLGKN
IMGSGFDFELIVHTGAGRYICGEETALINSLEGRRANPRSKPPFPASSGVWGKPTCVNNVETLCNVPAILANGVEWYKGI
SAGKSNDAGTKLMGFSGRVKNPGVWELPFGTTAREILEDYAGGMRDGLRFKAWQPGGAGTDFLTADHLDLPMDFESIGKA
GSRLGTALAMAVDHEIGMVPLVRNLEEFFSRESCGWCTPCRDGLPWSVKILRALENGEGQPGDIETLEQLCRSLGPGKTF
CAHAPGAVEPLQSAIKYFREEFEAGIKVKHFSNAHAIGGIQANNLLKQRW

Sequences:

>Translated_450_residues
MIKDIVRTPEMHPLTWRLRDDKQPVWLDEYRSKNGYAGAEKAIKGLAPDEIVTLVKDAGLKGRGGAGFSTGLKWSLMPKD
ESMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVHLRRAIAEATEAGYLGKN
IMGSGFDFELIVHTGAGRYICGEETALINSLEGRRANPRSKPPFPASSGVWGKPTCVNNVETLCNVPAILANGVEWYKGI
SAGKSNDAGTKLMGFSGRVKNPGVWELPFGTTAREILEDYAGGMRDGLRFKAWQPGGAGTDFLTADHLDLPMDFESIGKA
GSRLGTALAMAVDHEIGMVPLVRNLEEFFSRESCGWCTPCRDGLPWSVKILRALENGEGQPGDIETLEQLCRSLGPGKTF
CAHAPGAVEPLQSAIKYFREEFEAGIKVKHFSNAHAIGGIQANNLLKQRW
>Mature_450_residues
MIKDIVRTPEMHPLTWRLRDDKQPVWLDEYRSKNGYAGAEKAIKGLAPDEIVTLVKDAGLKGRGGAGFSTGLKWSLMPKD
ESMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVHLRRAIAEATEAGYLGKN
IMGSGFDFELIVHTGAGRYICGEETALINSLEGRRANPRSKPPFPASSGVWGKPTCVNNVETLCNVPAILANGVEWYKGI
SAGKSNDAGTKLMGFSGRVKNPGVWELPFGTTAREILEDYAGGMRDGLRFKAWQPGGAGTDFLTADHLDLPMDFESIGKA
GSRLGTALAMAVDHEIGMVPLVRNLEEFFSRESCGWCTPCRDGLPWSVKILRALENGEGQPGDIETLEQLCRSLGPGKTF
CAHAPGAVEPLQSAIKYFREEFEAGIKVKHFSNAHAIGGIQANNLLKQRW

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=390, Percent_Identity=43.8461538461538, Blast_Score=314, Evalue=1e-85,
Organism=Homo sapiens, GI260656005, Length=390, Percent_Identity=43.8461538461538, Blast_Score=314, Evalue=1e-85,
Organism=Escherichia coli, GI1788620, Length=449, Percent_Identity=90.8685968819599, Blast_Score=834, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17531727, Length=401, Percent_Identity=41.645885286783, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI19920794, Length=402, Percent_Identity=43.0348258706468, Blast_Score=315, Evalue=4e-86,
Organism=Drosophila melanogaster, GI45550452, Length=381, Percent_Identity=44.6194225721785, Blast_Score=311, Evalue=5e-85,
Organism=Drosophila melanogaster, GI24653819, Length=379, Percent_Identity=34.3007915567282, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24653817, Length=354, Percent_Identity=32.4858757062147, Blast_Score=205, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 49367; Mature: 49367

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKDIVRTPEMHPLTWRLRDDKQPVWLDEYRSKNGYAGAEKAIKGLAPDEIVTLVKDAGL
CCCCHHCCCCCCCEEEEECCCCCCCCHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCCC
KGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLIS
CCCCCCCCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AFALKAYRGYIFLRGEYIEAAVHLRRAIAEATEAGYLGKNIMGSGFDFELIVHTGAGRYI
HHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCEEEEEECCCCCEE
CGEETALINSLEGRRANPRSKPPFPASSGVWGKPTCVNNVETLCNVPAILANGVEWYKGI
ECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHCCHHHHHCC
SAGKSNDAGTKLMGFSGRVKNPGVWELPFGTTAREILEDYAGGMRDGLRFKAWQPGGAGT
CCCCCCCCCCEEECCCCCCCCCCEEECCCCCHHHHHHHHHCCCHHCCCEEEEECCCCCCC
DFLTADHLDLPMDFESIGKAGSRLGTALAMAVDHEIGMVPLVRNLEEFFSRESCGWCTPC
CCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCC
RDGLPWSVKILRALENGEGQPGDIETLEQLCRSLGPGKTFCAHAPGAVEPLQSAIKYFRE
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHH
EFEAGIKVKHFSNAHAIGGIQANNLLKQRW
HHHCCEEEEECCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MIKDIVRTPEMHPLTWRLRDDKQPVWLDEYRSKNGYAGAEKAIKGLAPDEIVTLVKDAGL
CCCCHHCCCCCCCEEEEECCCCCCCCHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCCC
KGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLIS
CCCCCCCCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AFALKAYRGYIFLRGEYIEAAVHLRRAIAEATEAGYLGKNIMGSGFDFELIVHTGAGRYI
HHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCEEEEEECCCCCEE
CGEETALINSLEGRRANPRSKPPFPASSGVWGKPTCVNNVETLCNVPAILANGVEWYKGI
ECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHCCHHHHHCC
SAGKSNDAGTKLMGFSGRVKNPGVWELPFGTTAREILEDYAGGMRDGLRFKAWQPGGAGT
CCCCCCCCCCEEECCCCCCCCCCEEECCCCCHHHHHHHHHCCCHHCCCEEEEECCCCCCC
DFLTADHLDLPMDFESIGKAGSRLGTALAMAVDHEIGMVPLVRNLEEFFSRESCGWCTPC
CCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCC
RDGLPWSVKILRALENGEGQPGDIETLEQLCRSLGPGKTFCAHAPGAVEPLQSAIKYFRE
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHH
EFEAGIKVKHFSNAHAIGGIQANNLLKQRW
HHHCCEEEEECCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7690854; 8157582; 9205837; 9278503; 8366049 [H]