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The map label for this gene is nuoE [H]

Identifier: 157371542

GI number: 157371542

Start: 3659920

End: 3660471

Strand: Reverse

Name: nuoE [H]

Synonym: Spro_3305

Alternate gene names: 157371542

Gene position: 3660471-3659920 (Counterclockwise)

Preceding gene: 157371543

Following gene: 157371541

Centisome position: 67.18

GC content: 53.99

Gene sequence:

>552_bases
ATGCATGATCAAAAAGACAATCACGCGAATAACCAAGCGCTTGAACCTATCAATGCGGCCGCTCCCCAGAGCGGCGTTGA
TGCATTTGAGCTGAGTGCGGAAGAACGTGATGCGATCGAGCACGAAAAGCACCATTACGAGGATCCACGCGCTGCCTCCA
TTGAAGCACTGAAAATTGTGCAGAAGCAGCGTGGCTGGGTTCCGGATGGGGCGATTCATGCCATCGCGGAGCTGCTGGGC
ATTCCTGCCAGCGACGTAGAAGGCGTGGCCACGTTCTACAGCCAGATTTTCCGTCAGCCAGTAGGGCGCCACGTGATCCG
TTATTGTGACAGCGTTGTTTGTCACATCACCGGTTACCAGGGCATTCAGGCCGCCATTGAGAAGAAGCTGAGCATCAAAC
CGGGTCAAACCACCTTTGATGGCCGTTTCACTCTGCTGCCAACCTGCTGCCTGGGCAACTGCGATAAAGGGCCAACCATG
ATGATTGATGAGGACACTCACAGTCAGCTGAAGCCTGAAGATATCGAGACGCTACTGGAGCAGTATCAATGA

Upstream 100 bases:

>100_bases
TGCAGCAAATCCCAGCGGTAATCCGTGGCAGCCTGGTCTCCGACCTGATCGTCTATCTGGGTAGTATCGATTTTGTAATG
TCAGATGTGGACCGCTAACT

Downstream 100 bases:

>100_bases
TTAAAGACATTGTACGCACTCCCGAAATGCACCCGCTGACCTGGCGGCTGCGCGATGACAAACAGCCGGTCTGGCTGGAT
GAATATCGCAGCAAGAACGG

Product: NADH dehydrogenase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E; NUO5 [H]

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MHDQKDNHANNQALEPINAAAPQSGVDAFELSAEERDAIEHEKHHYEDPRAASIEALKIVQKQRGWVPDGAIHAIAELLG
IPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHITGYQGIQAAIEKKLSIKPGQTTFDGRFTLLPTCCLGNCDKGPTM
MIDEDTHSQLKPEDIETLLEQYQ

Sequences:

>Translated_183_residues
MHDQKDNHANNQALEPINAAAPQSGVDAFELSAEERDAIEHEKHHYEDPRAASIEALKIVQKQRGWVPDGAIHAIAELLG
IPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHITGYQGIQAAIEKKLSIKPGQTTFDGRFTLLPTCCLGNCDKGPTM
MIDEDTHSQLKPEDIETLLEQYQ
>Mature_183_residues
MHDQKDNHANNQALEPINAAAPQSGVDAFELSAEERDAIEHEKHHYEDPRAASIEALKIVQKQRGWVPDGAIHAIAELLG
IPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHITGYQGIQAAIEKKLSIKPGQTTFDGRFTLLPTCCLGNCDKGPTM
MIDEDTHSQLKPEDIETLLEQYQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=166, Percent_Identity=34.9397590361446, Blast_Score=107, Evalue=4e-24,
Organism=Escherichia coli, GI1788621, Length=161, Percent_Identity=86.9565217391304, Blast_Score=298, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI17561328, Length=157, Percent_Identity=35.6687898089172, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI18859877, Length=157, Percent_Identity=34.3949044585987, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21355353, Length=166, Percent_Identity=29.5180722891566, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 20305; Mature: 20305

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDQKDNHANNQALEPINAAAPQSGVDAFELSAEERDAIEHEKHHYEDPRAASIEALKIV
CCCCCCCCCCCHHCCCHHHCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
QKQRGWVPDGAIHAIAELLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHITGYQ
HHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
GIQAAIEKKLSIKPGQTTFDGRFTLLPTCCLGNCDKGPTMMIDEDTHSQLKPEDIETLLE
HHHHHHHHHHCCCCCCCCCCCCEEEEHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHH
QYQ
HCC
>Mature Secondary Structure
MHDQKDNHANNQALEPINAAAPQSGVDAFELSAEERDAIEHEKHHYEDPRAASIEALKIV
CCCCCCCCCCCHHCCCHHHCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
QKQRGWVPDGAIHAIAELLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHITGYQ
HHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
GIQAAIEKKLSIKPGQTTFDGRFTLLPTCCLGNCDKGPTMMIDEDTHSQLKPEDIETLLE
HHHHHHHHHHCCCCCCCCCCCCEEEEHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHH
QYQ
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]