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The map label for this gene is menF [H]

Identifier: 157371521

GI number: 157371521

Start: 3627926

End: 3629224

Strand: Reverse

Name: menF [H]

Synonym: Spro_3284

Alternate gene names: 157371521

Gene position: 3629224-3627926 (Counterclockwise)

Preceding gene: 157371523

Following gene: 157371520

Centisome position: 66.61

GC content: 60.2

Gene sequence:

>1299_bases
GTGGTCGTGGAACAACTTTCGGGATTGCTGAGGCGGTTACGCCAGCAGCTTGAGCAACATTTGCCTTCTGATGGCGGTTT
CCGGCAGTTTGCACTGCCGGTACCGGCGCCGTTGGCCACCCGGTTGCTGGAATGGCTGGCGGTGCAACCGGCATTCCCGC
AGTTTTACTGGCGTCATCGCGACGGGCATGAGGAAGCGGCGGTCTGCGGCTCTGTGCGTCAGTTTACCCAGCCACGACAG
GCACAGGCGTTTCTCAGGACGCATGAGGGAGCGCGAGTCTGGGGACTGAATGCTTTTGACGGCGTGGCGCATTTATTTTT
GCCCCGTCTGGAAATTCTGAGCCGTGACGGTGAAACACAATTAACGCTGAATCTGTTTTCTGAAAGCTCGCTGCGTGAAG
ATGCGGCGTTTGCCGGCCGCTGGCTGGAGCAGGTATCGGCTGCAAGGCCCATCACGCCATTAAAGGCGCAGGTGGCAAAG
GTGCAGCATCGACCGGAACGGGCTGGGTGGGAAATCATGCTGCAAGACGCGCTGAAAGCGATAGGCCGGCAACAGATGGA
GAAGGTGGTGTTGGCGCGCAGTACGTTACTGACGCTGGATTGTCCGCTGTCTGCACCGGCGATGATGGATGCCAGCCGCA
GCGTGAACCACCGATGCTACCACTTTATGCTGCGCTTTGATGCACATTCCGCATTTCTCGGCTCCAGCCCGGAAAGACTG
TATCTGCGAAACGGCCGCCATCTGCTGACCGAAGCGCTGGCGGGCACCGTCGCCAATCACCCCGATCGGCCGCAGGCCCA
GGCGCTGGCGCAGTGGTTACTGCAGGACGGCAAGAACCAGCATGAGAATCTGCTGGTGGTGGATGATATCTGCCAGCGTC
TGCAAGGCGGTGCCGACGCGGTGGATGTGTTGCCGCCCGAAGTGGTGCGCTTGCGTAAGGTGCAGCATTTGCGGCGGCGT
ATTGAAGGCCGGCTCAACGATAACGACGATGCCGATTGCCTGCAGCGGTTACAGCCCACGGCGGCGGTAGCCGGGTTGCC
GCGTCAGGCTGCGCGCGAGTTTATTTTGCAACATGAGCCTTTCCCCCGTGGTTGGTATGCCGGATCTGCCGGCTATCTTT
CCTCCTCCCGCAGTGAGTTTTGCGTAGCGCTGCGCTGCGCGCAAATTGACGACCAGGAATTACGACTCTATGCCGGTGCC
GGCATTGTGGCCGGTTCTGACGCCGAGGCAGAGTGGCAGGAAATCGAAAACAAGGCCGCCGGGCTGCTGACTTTACTGCT
GCCAGAGCCATTGCGTTGA

Upstream 100 bases:

>100_bases
TAAACAATTGGCCGCTACTGACGTTGACTCCCAACGCTATTTTTCCGCCGTTCTGGCTTGATTTGCCGTATAATTCCCTA
CATTAAGCTTGGTTATTGAG

Downstream 100 bases:

>100_bases
ATTTCCCTGCTTATGCTCCGGATTTATTGAGCGAATGGCGCGGTGAGTGTAAACTGCTTGTGATTTTTATGTTTATTTTT
TGTTCTAAATCAATTAATTG

Product: isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MVVEQLSGLLRRLRQQLEQHLPSDGGFRQFALPVPAPLATRLLEWLAVQPAFPQFYWRHRDGHEEAAVCGSVRQFTQPRQ
AQAFLRTHEGARVWGLNAFDGVAHLFLPRLEILSRDGETQLTLNLFSESSLREDAAFAGRWLEQVSAARPITPLKAQVAK
VQHRPERAGWEIMLQDALKAIGRQQMEKVVLARSTLLTLDCPLSAPAMMDASRSVNHRCYHFMLRFDAHSAFLGSSPERL
YLRNGRHLLTEALAGTVANHPDRPQAQALAQWLLQDGKNQHENLLVVDDICQRLQGGADAVDVLPPEVVRLRKVQHLRRR
IEGRLNDNDDADCLQRLQPTAAVAGLPRQAAREFILQHEPFPRGWYAGSAGYLSSSRSEFCVALRCAQIDDQELRLYAGA
GIVAGSDAEAEWQEIENKAAGLLTLLLPEPLR

Sequences:

>Translated_432_residues
MVVEQLSGLLRRLRQQLEQHLPSDGGFRQFALPVPAPLATRLLEWLAVQPAFPQFYWRHRDGHEEAAVCGSVRQFTQPRQ
AQAFLRTHEGARVWGLNAFDGVAHLFLPRLEILSRDGETQLTLNLFSESSLREDAAFAGRWLEQVSAARPITPLKAQVAK
VQHRPERAGWEIMLQDALKAIGRQQMEKVVLARSTLLTLDCPLSAPAMMDASRSVNHRCYHFMLRFDAHSAFLGSSPERL
YLRNGRHLLTEALAGTVANHPDRPQAQALAQWLLQDGKNQHENLLVVDDICQRLQGGADAVDVLPPEVVRLRKVQHLRRR
IEGRLNDNDDADCLQRLQPTAAVAGLPRQAAREFILQHEPFPRGWYAGSAGYLSSSRSEFCVALRCAQIDDQELRLYAGA
GIVAGSDAEAEWQEIENKAAGLLTLLLPEPLR
>Mature_432_residues
MVVEQLSGLLRRLRQQLEQHLPSDGGFRQFALPVPAPLATRLLEWLAVQPAFPQFYWRHRDGHEEAAVCGSVRQFTQPRQ
AQAFLRTHEGARVWGLNAFDGVAHLFLPRLEILSRDGETQLTLNLFSESSLREDAAFAGRWLEQVSAARPITPLKAQVAK
VQHRPERAGWEIMLQDALKAIGRQQMEKVVLARSTLLTLDCPLSAPAMMDASRSVNHRCYHFMLRFDAHSAFLGSSPERL
YLRNGRHLLTEALAGTVANHPDRPQAQALAQWLLQDGKNQHENLLVVDDICQRLQGGADAVDVLPPEVVRLRKVQHLRRR
IEGRLNDNDDADCLQRLQPTAAVAGLPRQAAREFILQHEPFPRGWYAGSAGYLSSSRSEFCVALRCAQIDDQELRLYAGA
GIVAGSDAEAEWQEIENKAAGLLTLLLPEPLR

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=428, Percent_Identity=54.4392523364486, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1786809, Length=293, Percent_Identity=30.3754266211604, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1788114, Length=216, Percent_Identity=31.0185185185185, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787518, Length=276, Percent_Identity=27.536231884058, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320935, Length=217, Percent_Identity=30.4147465437788, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 48438; Mature: 48438

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVEQLSGLLRRLRQQLEQHLPSDGGFRQFALPVPAPLATRLLEWLAVQPAFPQFYWRHR
CCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHCC
DGHEEAAVCGSVRQFTQPRQAQAFLRTHEGARVWGLNAFDGVAHLFLPRLEILSRDGETQ
CCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCE
LTLNLFSESSLREDAAFAGRWLEQVSAARPITPLKAQVAKVQHRPERAGWEIMLQDALKA
EEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
IGRQQMEKVVLARSTLLTLDCPLSAPAMMDASRSVNHRCYHFMLRFDAHSAFLGSSPERL
HHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHEECCHHHHHCCCCCEE
YLRNGRHLLTEALAGTVANHPDRPQAQALAQWLLQDGKNQHENLLVVDDICQRLQGGADA
EHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCCCE
VDVLPPEVVRLRKVQHLRRRIEGRLNDNDDADCLQRLQPTAAVAGLPRQAAREFILQHEP
ECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCHHHHHHCCCHHHHHHHHHCCCC
FPRGWYAGSAGYLSSSRSEFCVALRCAQIDDQELRLYAGAGIVAGSDAEAEWQEIENKAA
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCHHHHHHHHHHH
GLLTLLLPEPLR
HEEEEECCCCCC
>Mature Secondary Structure
MVVEQLSGLLRRLRQQLEQHLPSDGGFRQFALPVPAPLATRLLEWLAVQPAFPQFYWRHR
CCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHCC
DGHEEAAVCGSVRQFTQPRQAQAFLRTHEGARVWGLNAFDGVAHLFLPRLEILSRDGETQ
CCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCE
LTLNLFSESSLREDAAFAGRWLEQVSAARPITPLKAQVAKVQHRPERAGWEIMLQDALKA
EEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
IGRQQMEKVVLARSTLLTLDCPLSAPAMMDASRSVNHRCYHFMLRFDAHSAFLGSSPERL
HHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHEECCHHHHHCCCCCEE
YLRNGRHLLTEALAGTVANHPDRPQAQALAQWLLQDGKNQHENLLVVDDICQRLQGGADA
EHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCCCE
VDVLPPEVVRLRKVQHLRRRIEGRLNDNDDADCLQRLQPTAAVAGLPRQAAREFILQHEP
ECCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCHHHHHHCCCHHHHHHHHHCCCC
FPRGWYAGSAGYLSSSRSEFCVALRCAQIDDQELRLYAGAGIVAGSDAEAEWQEIENKAA
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCHHHHHHHHHHH
GLLTLLLPEPLR
HEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8549818; 8764478; 9205837; 9278503; 2666397; 7984428 [H]