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The map label for this gene is menD

Identifier: 157371520

GI number: 157371520

Start: 3626000

End: 3627673

Strand: Reverse

Name: menD

Synonym: Spro_3283

Alternate gene names: 157371520

Gene position: 3627673-3626000 (Counterclockwise)

Preceding gene: 157371521

Following gene: 157371519

Centisome position: 66.58

GC content: 62.6

Gene sequence:

>1674_bases
ATGTCGACAAGTGTTTTTAATCGCCGGTGGGCGGCGTTGCTGTTGGAAGCGCTGGCCCGCCATGGAGTGCGACATGTCTG
TATCGCTCCGGGATCGCGCTCTACGCCGTTGACGCTGGCCGCGGCAGCTAACAAGTCTTTTATCTGCCATACCCATTTTG
ATGAGCGCGGGCTGGGCCATTTGGCGCTTGGCCTGGCGAAGGCATCGCGCGAACCTGTGGCGGTGATCGTCACTTCCGGC
ACCGCCGCCGCTAACCTTTATCCCTCTTTGATCGAAGCCGGGCTGACCGGTGAACGGCTGGTGTTTCTTACCGCCGACCG
GCCGCCTGAACTGATCAACTGCGGTGCCAATCAGGCGATCCGCCAAAACGGCCTGTATGCCAGCCATCCGGCGCTGAGTA
TTGATCTGCCGCGGCCGACGACGGACATCCCTGCTCGCTGGTTGGTGTCCACCGTCGACAGCGCGATGGCACGCCTGCAG
CACGGCGCTTTACACATTAATTGCCCGTTTGCCGAACCCTTGTACGGCGGTGATGAGCAACAGTATGCCGACTGGTCGGC
GACGCTCGGCGACTGGTGGCAAGGCAACCACCCCTGGCTGCGGGAAGTGGATCCGCACCAGGTGCTCAAGCAACCGGACT
GGTTCTTCTGGCGGCAAAAGCGCGGCGTGATAGTGGCGGGGCGGATGAGCGCGGATGAGGGCGAACATCTGGCGCAGTGG
GCCGAGATGCTTGGCTGGCCGCTGATTGGCGACGTGCTGTCCCAGACCGGGCAGCCGCTGCCCTGTGCCGATTTATGGCT
GGCCCAGCCGCAGGCGCAAAAACGGCTGGCCGAGGCACAATTGGTGGTGCAGTTTGGCAGCAGCCTGACCGGCAAACGCC
TGCTGCAGTGGCAAGAGCAGTGTCAGCCGCAAGAGTATTGGCTGATCGACGATTTACCGGGGCGTCTCGATCCGGCGCAT
CATCGCGGGCGCAGGATCCGTTCCAGCGTGGCGCAGTGGCTCGAACTGCATCCGGCTCAGCCGCGAACCCCGTGGGCGGA
TGAACTGACGATGTTGGCGGACAACGCGCTGGATGCCGTCTCCGGCCATTTGGTTAACCGCTTTGGCGAGGCGCAGTTGG
CGCATCGCCTGCCGGAACTGCTGCCGGAAAACGGCCAACTGTTCCTCGGCAACAGCCTGATCGTACGGTTGATTGATGCG
TTGACCCGCTTGCCGGCCGGTTATCCGGTATTCAGCAACCGCGGGGCCAGCGGCATCGACGGTCTGATTTCCACTGCCGC
AGGCGTACAGCGGGCCACCGCCAAACCGACGCTGGCGGTGGTGGGAGATTTATCGGCACTGTATGACCTGAACGCGCTGG
CGCTGCTGCGCCAATGTTCTGCGCCGACGGTGCTGATTGTGGTTAATAATAACGGCGGCCAGATTTTCTCACTGTTGCCG
ACGCCGGAAGAAGACCGGCAACGGTTCTACTGCATGCCGCAGAACGTAGAGTTCAGCCATGCGGCGGCGATGTTCCAGCT
GGCTTATGCCCGCCCGGAAAACTGGGGGCAATTGCTGCAGGCGGTGGAACAGGGCTGGCGTCACGGTGGCGCTACGCTGA
TTGAGCTGCAGGTGCCGCCGAGCGATGGTGCGCAGACGCTGCAGCATCTGGTGCAACAGATGACGGAGCAATGA

Upstream 100 bases:

>100_bases
GTAAAGATAACTACTCATATAATGCAGAACATCATTTTTGGCATGCCGTCGATTGGCGGGCGCCAGGGGACGATTCTGTT
AACCGACTTGTGAGCGAATT

Downstream 100 bases:

>100_bases
TGCTGGCGACAAAAGTATTGCAACAGGGCGAAGCCGATCGCCCATGGCTAATCTGGTTACACGGCCTGTTGGGCAATAAT
AATGAATGGCGAGTGATCGC

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MSTSVFNRRWAALLLEALARHGVRHVCIAPGSRSTPLTLAAAANKSFICHTHFDERGLGHLALGLAKASREPVAVIVTSG
TAAANLYPSLIEAGLTGERLVFLTADRPPELINCGANQAIRQNGLYASHPALSIDLPRPTTDIPARWLVSTVDSAMARLQ
HGALHINCPFAEPLYGGDEQQYADWSATLGDWWQGNHPWLREVDPHQVLKQPDWFFWRQKRGVIVAGRMSADEGEHLAQW
AEMLGWPLIGDVLSQTGQPLPCADLWLAQPQAQKRLAEAQLVVQFGSSLTGKRLLQWQEQCQPQEYWLIDDLPGRLDPAH
HRGRRIRSSVAQWLELHPAQPRTPWADELTMLADNALDAVSGHLVNRFGEAQLAHRLPELLPENGQLFLGNSLIVRLIDA
LTRLPAGYPVFSNRGASGIDGLISTAAGVQRATAKPTLAVVGDLSALYDLNALALLRQCSAPTVLIVVNNNGGQIFSLLP
TPEEDRQRFYCMPQNVEFSHAAAMFQLAYARPENWGQLLQAVEQGWRHGGATLIELQVPPSDGAQTLQHLVQQMTEQ

Sequences:

>Translated_557_residues
MSTSVFNRRWAALLLEALARHGVRHVCIAPGSRSTPLTLAAAANKSFICHTHFDERGLGHLALGLAKASREPVAVIVTSG
TAAANLYPSLIEAGLTGERLVFLTADRPPELINCGANQAIRQNGLYASHPALSIDLPRPTTDIPARWLVSTVDSAMARLQ
HGALHINCPFAEPLYGGDEQQYADWSATLGDWWQGNHPWLREVDPHQVLKQPDWFFWRQKRGVIVAGRMSADEGEHLAQW
AEMLGWPLIGDVLSQTGQPLPCADLWLAQPQAQKRLAEAQLVVQFGSSLTGKRLLQWQEQCQPQEYWLIDDLPGRLDPAH
HRGRRIRSSVAQWLELHPAQPRTPWADELTMLADNALDAVSGHLVNRFGEAQLAHRLPELLPENGQLFLGNSLIVRLIDA
LTRLPAGYPVFSNRGASGIDGLISTAAGVQRATAKPTLAVVGDLSALYDLNALALLRQCSAPTVLIVVNNNGGQIFSLLP
TPEEDRQRFYCMPQNVEFSHAAAMFQLAYARPENWGQLLQAVEQGWRHGGATLIELQVPPSDGAQTLQHLVQQMTEQ
>Mature_556_residues
STSVFNRRWAALLLEALARHGVRHVCIAPGSRSTPLTLAAAANKSFICHTHFDERGLGHLALGLAKASREPVAVIVTSGT
AAANLYPSLIEAGLTGERLVFLTADRPPELINCGANQAIRQNGLYASHPALSIDLPRPTTDIPARWLVSTVDSAMARLQH
GALHINCPFAEPLYGGDEQQYADWSATLGDWWQGNHPWLREVDPHQVLKQPDWFFWRQKRGVIVAGRMSADEGEHLAQWA
EMLGWPLIGDVLSQTGQPLPCADLWLAQPQAQKRLAEAQLVVQFGSSLTGKRLLQWQEQCQPQEYWLIDDLPGRLDPAHH
RGRRIRSSVAQWLELHPAQPRTPWADELTMLADNALDAVSGHLVNRFGEAQLAHRLPELLPENGQLFLGNSLIVRLIDAL
TRLPAGYPVFSNRGASGIDGLISTAAGVQRATAKPTLAVVGDLSALYDLNALALLRQCSAPTVLIVVNNNGGQIFSLLPT
PEEDRQRFYCMPQNVEFSHAAAMFQLAYARPENWGQLLQAVEQGWRHGGATLIELQVPPSDGAQTLQHLVQQMTEQ

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=555, Percent_Identity=71.3513513513514, Blast_Score=821, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_SERP5 (A8GGZ1)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479509.1
- ProteinModelPortal:   A8GGZ1
- STRING:   A8GGZ1
- GeneID:   5607103
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3283
- eggNOG:   COG1165
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- ProtClustDB:   PRK07449
- BioCyc:   SPRO399741:SPRO_3283-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 61273; Mature: 61142

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSVFNRRWAALLLEALARHGVRHVCIAPGSRSTPLTLAAAANKSFICHTHFDERGLGH
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCCCHH
LALGLAKASREPVAVIVTSGTAAANLYPSLIEAGLTGERLVFLTADRPPELINCGANQAI
HHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHCCCCCHHH
RQNGLYASHPALSIDLPRPTTDIPARWLVSTVDSAMARLQHGALHINCPFAEPLYGGDEQ
HHCCCEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCH
QYADWSATLGDWWQGNHPWLREVDPHQVLKQPDWFFWRQKRGVIVAGRMSADEGEHLAQW
HHCCHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCCEEEEECCCCCCHHHHHHH
AEMLGWPLIGDVLSQTGQPLPCADLWLAQPQAQKRLAEAQLVVQFGSSLTGKRLLQWQEQ
HHHHCCHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
CQPQEYWLIDDLPGRLDPAHHRGRRIRSSVAQWLELHPAQPRTPWADELTMLADNALDAV
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
SGHLVNRFGEAQLAHRLPELLPENGQLFLGNSLIVRLIDALTRLPAGYPVFSNRGASGID
HHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
GLISTAAGVQRATAKPTLAVVGDLSALYDLNALALLRQCSAPTVLIVVNNNGGQIFSLLP
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECC
TPEEDRQRFYCMPQNVEFSHAAAMFQLAYARPENWGQLLQAVEQGWRHGGATLIELQVPP
CCHHHCCEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECCC
SDGAQTLQHLVQQMTEQ
CCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STSVFNRRWAALLLEALARHGVRHVCIAPGSRSTPLTLAAAANKSFICHTHFDERGLGH
CCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCCCHH
LALGLAKASREPVAVIVTSGTAAANLYPSLIEAGLTGERLVFLTADRPPELINCGANQAI
HHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHCCCCCHHH
RQNGLYASHPALSIDLPRPTTDIPARWLVSTVDSAMARLQHGALHINCPFAEPLYGGDEQ
HHCCCEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCH
QYADWSATLGDWWQGNHPWLREVDPHQVLKQPDWFFWRQKRGVIVAGRMSADEGEHLAQW
HHCCHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCCEEEEECCCCCCHHHHHHH
AEMLGWPLIGDVLSQTGQPLPCADLWLAQPQAQKRLAEAQLVVQFGSSLTGKRLLQWQEQ
HHHHCCHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
CQPQEYWLIDDLPGRLDPAHHRGRRIRSSVAQWLELHPAQPRTPWADELTMLADNALDAV
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
SGHLVNRFGEAQLAHRLPELLPENGQLFLGNSLIVRLIDALTRLPAGYPVFSNRGASGID
HHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
GLISTAAGVQRATAKPTLAVVGDLSALYDLNALALLRQCSAPTVLIVVNNNGGQIFSLLP
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECC
TPEEDRQRFYCMPQNVEFSHAAAMFQLAYARPENWGQLLQAVEQGWRHGGATLIELQVPP
CCHHHCCEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECCC
SDGAQTLQHLVQQMTEQ
CCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA