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The map label for this gene is braE [H]

Identifier: 157371438

GI number: 157371438

Start: 3524650

End: 3525936

Strand: Reverse

Name: braE [H]

Synonym: Spro_3201

Alternate gene names: 157371438

Gene position: 3525936-3524650 (Counterclockwise)

Preceding gene: 157371439

Following gene: 157371437

Centisome position: 64.71

GC content: 60.76

Gene sequence:

>1287_bases
ATGTCGCAATCTATGGTTCACCCAGGGCTGAACGTTAAACGCAGCCTGCTGGATGCGGTGCTGGCCGGGCTGATCGCCCT
GATCGTCTTCGGGCCGATCGTCGGTATTGTTCTCAACGGATACAGTTTTAACTTTGAACCGCGCCGCCTGGCGTGGATTA
TCGCCGCGGTAATGGCCGGGCGGCTGTTGCTCAGCCTGTTCCTGCAAACCGCGCCGGGGCGGAAATTGTTGACGCGTTTT
GACGGCGGCAATGACGGGGTGTATGTCCGGCCGATAGGGGCCAAAACCAGTCTGCGATGGATCATCCCGTTAATGGCGGT
GATCGCGCTGCTGTTTCCGTTTGTCGCCACCAAGTACCTGCTGACGGTGGCGATCCTGGGGCTGATTTACGTGCTGCTTG
GGCTGGGGTTGAACATTGTTGTCGGGCTGGCCGGGCTGCTGGATCTCGGTTATGTGGCGTTCTACGCCATTGGCGCTTAT
GGCCTGGCGCTGGGTTATCAGTATCTCGGGCTGGGGTTCTGGGCGATGTTGCCGCTGGGGGCGCTGATGGCCGCGCTGGC
CGGTGCGCTGCTGGGGTTCCCGGTGCTGCGCATGCACGGTGACTATCTGGCGATCGTCACGCTGGGGTTCGGGGAAATTA
TCCGCCTGGTGCTGAATAACTGGATGTCGCTGACCGGTGGCCCCAACGGCGTCTCGGTGCCTGCGCCGACGATTTTTGGC
CTGGAATTTGGCCGACGGGCGAAGGACGGCGGGGTGCCGATCCACGAATTCCTCGGTATCGCCTATAACCCCAACCTGAA
ATTTATCTTTATCTATGCGGTGCTGTTCCTGGTGGTGCTGCTGGTGCTGTACATCAAGCACCGGCTGACGCGCATGCCGA
TCGGCCGCGCCTGGGAGGCACTGCGCGAGGATGAAATCGCCTGTCGCTCGCTCGGGCTGAACCACGTTCTGGTGAAGCTG
TCGGCCTTTATGATGGGTGCCTCTACCGCCGGGCTGGCCGGGGTGTTTTTTGCCACTTATCAGGGCTTCGTCAACCCCAC
CTCATTCACCTTCTTTGAGTCGGCGCTGATCCTGGCGATCGTGGTGCTGGGCGGTATGGGCTCGACCATCGGCGTAGTGC
TGGCGGCGTTTGTACTCACGGTGGCACCTGAGCTGTTACGTAGTTTTGCCGAGTACCGGGTGCTGCTGTTTGGCGTGTTG
ATGGTGGCGATGATGATCTGGCGGCCGCGTGGGCTGGTGCGCATCAGTCGTTCCAGTTTTGCTCCGCGCAAGGGGGTAGA
GCCATGA

Upstream 100 bases:

>100_bases
AAAGACGTATTCTCCTTCGGTTTGCTGGTGGTGATCCTGATTTTCCGCCCTCAGGGCCTGCTCGGTCGGCCAATGGTCGC
TAAGGTTTGAGGGGAAAACT

Downstream 100 bases:

>100_bases
GCGACGCTATCTTGCAGGTTGAGCATCTGATGATGCATTTTGGCGGTATCAAGGCGCTGAATGACGTGAATCTGGCGGTA
GAGCGCGGTTCCATCACCGC

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSQSMVHPGLNVKRSLLDAVLAGLIALIVFGPIVGIVLNGYSFNFEPRRLAWIIAAVMAGRLLLSLFLQTAPGRKLLTRF
DGGNDGVYVRPIGAKTSLRWIIPLMAVIALLFPFVATKYLLTVAILGLIYVLLGLGLNIVVGLAGLLDLGYVAFYAIGAY
GLALGYQYLGLGFWAMLPLGALMAALAGALLGFPVLRMHGDYLAIVTLGFGEIIRLVLNNWMSLTGGPNGVSVPAPTIFG
LEFGRRAKDGGVPIHEFLGIAYNPNLKFIFIYAVLFLVVLLVLYIKHRLTRMPIGRAWEALREDEIACRSLGLNHVLVKL
SAFMMGASTAGLAGVFFATYQGFVNPTSFTFFESALILAIVVLGGMGSTIGVVLAAFVLTVAPELLRSFAEYRVLLFGVL
MVAMMIWRPRGLVRISRSSFAPRKGVEP

Sequences:

>Translated_428_residues
MSQSMVHPGLNVKRSLLDAVLAGLIALIVFGPIVGIVLNGYSFNFEPRRLAWIIAAVMAGRLLLSLFLQTAPGRKLLTRF
DGGNDGVYVRPIGAKTSLRWIIPLMAVIALLFPFVATKYLLTVAILGLIYVLLGLGLNIVVGLAGLLDLGYVAFYAIGAY
GLALGYQYLGLGFWAMLPLGALMAALAGALLGFPVLRMHGDYLAIVTLGFGEIIRLVLNNWMSLTGGPNGVSVPAPTIFG
LEFGRRAKDGGVPIHEFLGIAYNPNLKFIFIYAVLFLVVLLVLYIKHRLTRMPIGRAWEALREDEIACRSLGLNHVLVKL
SAFMMGASTAGLAGVFFATYQGFVNPTSFTFFESALILAIVVLGGMGSTIGVVLAAFVLTVAPELLRSFAEYRVLLFGVL
MVAMMIWRPRGLVRISRSSFAPRKGVEP
>Mature_427_residues
SQSMVHPGLNVKRSLLDAVLAGLIALIVFGPIVGIVLNGYSFNFEPRRLAWIIAAVMAGRLLLSLFLQTAPGRKLLTRFD
GGNDGVYVRPIGAKTSLRWIIPLMAVIALLFPFVATKYLLTVAILGLIYVLLGLGLNIVVGLAGLLDLGYVAFYAIGAYG
LALGYQYLGLGFWAMLPLGALMAALAGALLGFPVLRMHGDYLAIVTLGFGEIIRLVLNNWMSLTGGPNGVSVPAPTIFGL
EFGRRAKDGGVPIHEFLGIAYNPNLKFIFIYAVLFLVVLLVLYIKHRLTRMPIGRAWEALREDEIACRSLGLNHVLVKLS
AFMMGASTAGLAGVFFATYQGFVNPTSFTFFESALILAIVVLGGMGSTIGVVLAAFVLTVAPELLRSFAEYRVLLFGVLM
VAMMIWRPRGLVRISRSSFAPRKGVEP

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=316, Percent_Identity=58.8607594936709, Blast_Score=342, Evalue=2e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 46211; Mature: 46080

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSMVHPGLNVKRSLLDAVLAGLIALIVFGPIVGIVLNGYSFNFEPRRLAWIIAAVMAG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RLLLSLFLQTAPGRKLLTRFDGGNDGVYVRPIGAKTSLRWIIPLMAVIALLFPFVATKYL
HHHHHHHHHCCCCHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LTVAILGLIYVLLGLGLNIVVGLAGLLDLGYVAFYAIGAYGLALGYQYLGLGFWAMLPLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALMAALAGALLGFPVLRMHGDYLAIVTLGFGEIIRLVLNNWMSLTGGPNGVSVPAPTIFG
HHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCC
LEFGRRAKDGGVPIHEFLGIAYNPNLKFIFIYAVLFLVVLLVLYIKHRLTRMPIGRAWEA
CHHCCCCCCCCCCHHHHHCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LREDEIACRSLGLNHVLVKLSAFMMGASTAGLAGVFFATYQGFVNPTSFTFFESALILAI
HHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VVLGGMGSTIGVVLAAFVLTVAPELLRSFAEYRVLLFGVLMVAMMIWRPRGLVRISRSSF
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
APRKGVEP
CCCCCCCC
>Mature Secondary Structure 
SQSMVHPGLNVKRSLLDAVLAGLIALIVFGPIVGIVLNGYSFNFEPRRLAWIIAAVMAG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RLLLSLFLQTAPGRKLLTRFDGGNDGVYVRPIGAKTSLRWIIPLMAVIALLFPFVATKYL
HHHHHHHHHCCCCHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LTVAILGLIYVLLGLGLNIVVGLAGLLDLGYVAFYAIGAYGLALGYQYLGLGFWAMLPLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALMAALAGALLGFPVLRMHGDYLAIVTLGFGEIIRLVLNNWMSLTGGPNGVSVPAPTIFG
HHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCC
LEFGRRAKDGGVPIHEFLGIAYNPNLKFIFIYAVLFLVVLLVLYIKHRLTRMPIGRAWEA
CHHCCCCCCCCCCHHHHHCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LREDEIACRSLGLNHVLVKLSAFMMGASTAGLAGVFFATYQGFVNPTSFTFFESALILAI
HHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VVLGGMGSTIGVVLAAFVLTVAPELLRSFAEYRVLLFGVLMVAMMIWRPRGLVRISRSSF
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
APRKGVEP
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]