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The map label for this gene is braF [H]

Identifier: 157371437

GI number: 157371437

Start: 3523778

End: 3524653

Strand: Reverse

Name: braF [H]

Synonym: Spro_3200

Alternate gene names: 157371437

Gene position: 3524653-3523778 (Counterclockwise)

Preceding gene: 157371438

Following gene: 157371436

Centisome position: 64.69

GC content: 59.25

Gene sequence:

>876_bases
ATGAGCGACGCTATCTTGCAGGTTGAGCATCTGATGATGCATTTTGGCGGTATCAAGGCGCTGAATGACGTGAATCTGGC
GGTAGAGCGCGGTTCCATCACCGCGCTGATCGGCCCCAACGGTGCCGGTAAAACCACGGTATTCAACTGTCTGACCGGTT
TTTATCGCGCCACCGGCGGCGCGATCCTGCTTAATACCCACAAGCGGCCGACCGACGTGATCCAGGTGCTGGGGCAGAAA
TTCCACGCCGGTGACTGGGTTAACCCGAAACGCCTGGGCTCGCGCGTTTACTACAAAATGTTTGGCGGCACCCATCTGGT
CAATCGCGCCGGGCTGGCGCGCACCTTCCAGAATATCCGTTTGTTCCGCGAAATGTCGGTGGTGGAGAACCTGCTGGTGG
CGCAGCACATGCTGAGCAACCGCAATCTGATCGCCGGCGTGCTCAATACGCCGGGTTACCGCCGCGCAGAAAACCAGGCG
CTGGATCGCGCCTTTTACTGGCTGGAAGTGGTGGAGCTGGTGGATTGCGCCAACCGGCTGGCGGGGGAGATGTCCTACGG
CCAACAGCGGCGGCTGGAGATCGCCCGGGCAATGTGCACCACGCCGGAAATGATCTGTCTCGATGAACCCGCCGCCGGGC
TAAACCCGGTAGAGACGGCGACGCTGAGCCGCATTATCCGTTTTCTGCGTCAGCACCATGGCATCACGGTGCTGTTGATT
GAACACGATATGGGCATGGTGATGGAGATCTCCGATCGGGTGATTGTGCTCGATCACGGTGATGTGATCGCCGAAGGCAC
ACCGAAAGAAATTCAGCACAACGATACGGTGATCGCCGCTTACCTTGGCGCGGACGAAGAGGAACTGGCGGGATGA

Upstream 100 bases:

>100_bases
TGCTGTTTGGCGTGTTGATGGTGGCGATGATGATCTGGCGGCCGCGTGGGCTGGTGCGCATCAGTCGTTCCAGTTTTGCT
CCGCGCAAGGGGGTAGAGCC

Downstream 100 bases:

>100_bases
GCAATGTGATGTTGGAGTTTCGCGATGTAGACGTGTTTTATGGCCCGATCCAGGCGTTGCAGCAGGTCTCGCTACAGGTG
AATGAAGGCGAAACGGTGGC

Product: ABC transporter-like protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MSDAILQVEHLMMHFGGIKALNDVNLAVERGSITALIGPNGAGKTTVFNCLTGFYRATGGAILLNTHKRPTDVIQVLGQK
FHAGDWVNPKRLGSRVYYKMFGGTHLVNRAGLARTFQNIRLFREMSVVENLLVAQHMLSNRNLIAGVLNTPGYRRAENQA
LDRAFYWLEVVELVDCANRLAGEMSYGQQRRLEIARAMCTTPEMICLDEPAAGLNPVETATLSRIIRFLRQHHGITVLLI
EHDMGMVMEISDRVIVLDHGDVIAEGTPKEIQHNDTVIAAYLGADEEELAG

Sequences:

>Translated_291_residues
MSDAILQVEHLMMHFGGIKALNDVNLAVERGSITALIGPNGAGKTTVFNCLTGFYRATGGAILLNTHKRPTDVIQVLGQK
FHAGDWVNPKRLGSRVYYKMFGGTHLVNRAGLARTFQNIRLFREMSVVENLLVAQHMLSNRNLIAGVLNTPGYRRAENQA
LDRAFYWLEVVELVDCANRLAGEMSYGQQRRLEIARAMCTTPEMICLDEPAAGLNPVETATLSRIIRFLRQHHGITVLLI
EHDMGMVMEISDRVIVLDHGDVIAEGTPKEIQHNDTVIAAYLGADEEELAG
>Mature_290_residues
SDAILQVEHLMMHFGGIKALNDVNLAVERGSITALIGPNGAGKTTVFNCLTGFYRATGGAILLNTHKRPTDVIQVLGQKF
HAGDWVNPKRLGSRVYYKMFGGTHLVNRAGLARTFQNIRLFREMSVVENLLVAQHMLSNRNLIAGVLNTPGYRRAENQAL
DRAFYWLEVVELVDCANRLAGEMSYGQQRRLEIARAMCTTPEMICLDEPAAGLNPVETATLSRIIRFLRQHHGITVLLIE
HDMGMVMEISDRVIVLDHGDVIAEGTPKEIQHNDTVIAAYLGADEEELAG

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Escherichia coli, GI1789864, Length=283, Percent_Identity=51.9434628975265, Blast_Score=280, Evalue=9e-77,
Organism=Escherichia coli, GI1789593, Length=282, Percent_Identity=30.1418439716312, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI87082268, Length=284, Percent_Identity=29.5774647887324, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1789672, Length=272, Percent_Identity=25.7352941176471, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788761, Length=267, Percent_Identity=26.9662921348315, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1787029, Length=270, Percent_Identity=24.8148148148148, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87081709, Length=281, Percent_Identity=25.6227758007117, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790190, Length=263, Percent_Identity=27.7566539923954, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI48995001, Length=259, Percent_Identity=28.957528957529, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1786872, Length=263, Percent_Identity=27.3764258555133, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790544, Length=271, Percent_Identity=27.6752767527675, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788210, Length=281, Percent_Identity=28.1138790035587, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788225, Length=264, Percent_Identity=28.030303030303, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1786398, Length=263, Percent_Identity=28.1368821292776, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1787370, Length=271, Percent_Identity=25.830258302583, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787712, Length=271, Percent_Identity=25.0922509225092, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790525, Length=263, Percent_Identity=27.7566539923954, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789991, Length=273, Percent_Identity=26.7399267399267, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788450, Length=265, Percent_Identity=26.4150943396226, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1789032, Length=283, Percent_Identity=25.0883392226148, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI48994943, Length=266, Percent_Identity=27.8195488721804, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790467, Length=285, Percent_Identity=23.5087719298246, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787089, Length=261, Percent_Identity=25.2873563218391, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1789586, Length=254, Percent_Identity=27.9527559055118, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788644, Length=272, Percent_Identity=26.4705882352941, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787500, Length=259, Percent_Identity=25.8687258687259, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI145693107, Length=276, Percent_Identity=23.9130434782609, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1786345, Length=280, Percent_Identity=24.2857142857143, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787758, Length=282, Percent_Identity=23.0496453900709, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87082267, Length=272, Percent_Identity=24.2647058823529, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787759, Length=286, Percent_Identity=25.5244755244755, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1789891, Length=248, Percent_Identity=26.2096774193548, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI48994997, Length=269, Percent_Identity=25.6505576208178, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786703, Length=270, Percent_Identity=24.8148148148148, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787164, Length=249, Percent_Identity=25.3012048192771, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788897, Length=281, Percent_Identity=24.9110320284698, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1786803, Length=270, Percent_Identity=22.5925925925926, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788506, Length=92, Percent_Identity=34.7826086956522, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786981, Length=265, Percent_Identity=27.9245283018868, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71983516, Length=270, Percent_Identity=25.1851851851852, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71983510, Length=270, Percent_Identity=25.1851851851852, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71983523, Length=270, Percent_Identity=25.1851851851852, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 32233; Mature: 32102

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAILQVEHLMMHFGGIKALNDVNLAVERGSITALIGPNGAGKTTVFNCLTGFYRATGG
CCCHHHHHHHHHHHHCCCCEECCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCC
AILLNTHKRPTDVIQVLGQKFHAGDWVNPKRLGSRVYYKMFGGTHLVNRAGLARTFQNIR
EEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHCCHHEEECCCCHHHHHHHHHHHHHHHHH
LFREMSVVENLLVAQHMLSNRNLIAGVLNTPGYRRAENQALDRAFYWLEVVELVDCANRL
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AGEMSYGQQRRLEIARAMCTTPEMICLDEPAAGLNPVETATLSRIIRFLRQHHGITVLLI
HHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEE
EHDMGMVMEISDRVIVLDHGDVIAEGTPKEIQHNDTVIAAYLGADEEELAG
ECCCCEEEEECCCEEEEECCCEEECCCCHHHCCCCCEEEEEECCCHHHHCC
>Mature Secondary Structure 
SDAILQVEHLMMHFGGIKALNDVNLAVERGSITALIGPNGAGKTTVFNCLTGFYRATGG
CCHHHHHHHHHHHHCCCCEECCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCC
AILLNTHKRPTDVIQVLGQKFHAGDWVNPKRLGSRVYYKMFGGTHLVNRAGLARTFQNIR
EEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHCCHHEEECCCCHHHHHHHHHHHHHHHHH
LFREMSVVENLLVAQHMLSNRNLIAGVLNTPGYRRAENQALDRAFYWLEVVELVDCANRL
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AGEMSYGQQRRLEIARAMCTTPEMICLDEPAAGLNPVETATLSRIIRFLRQHHGITVLLI
HHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEE
EHDMGMVMEISDRVIVLDHGDVIAEGTPKEIQHNDTVIAAYLGADEEELAG
ECCCCEEEEECCCEEEEECCCEEECCCCHHHCCCCCEEEEEECCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]