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The map label for this gene is nikB [C]

Identifier: 157371395

GI number: 157371395

Start: 3474862

End: 3475806

Strand: Reverse

Name: nikB [C]

Synonym: Spro_3156

Alternate gene names: 157371395

Gene position: 3475806-3474862 (Counterclockwise)

Preceding gene: 157371396

Following gene: 157371394

Centisome position: 63.79

GC content: 61.38

Gene sequence:

>945_bases
ATGAGCCGATATCTGGCGCGGCGCATCGGGCAGGCGCTGCTGGTGCTGTGGGCGACCTTTAGCCTGTCGTTTCTTCTGCT
GCAGGTGCTGCCCGGCGATGCGATCCTGATTAAGTTCCAGAACCCGGACATGGGGCTAAGCCCGGCGCAGATCGCCGATA
TGCGCGCAGCCTATGGCGCTGATGTGTCGCTGTGGCAGCAGTATCTGCATGCGTTGGGTAACGTGCTGCGTGGGGATCTG
GGGTATTCCATTCAGGCCGGGGTGCCGGTCACCGAACTGTTAGCCACCAACCTGCCCGCCACGCTGCAACTGGCGGTGCT
GGGCTTCACGCTGGCGCTGCTTCTGGCACTGACGATTGCCTTTATCTCCAACCTGACCGGTTTTGGCTGGCTGAGAGCGG
CACTGCAGTCGCTGCCGTCGCTGTTTGTCTCGGTGCCGACCTTCTGGCTGGGGATTGTGCTGATCCAGGTGTTTTCGTTC
CAGCTCAAGCTGATCCCGGTGATCAACCCGGGTGAATGGCAGGGGCTGATCTTGCCGATTGTGACGCTGGCGGTACCGAT
CTCCGCCCCCTTGGCGCAGATCCTGATCCGTAGCATTGATCAGGTGCAGACTCAGCCGTTTGTCGCCGTGGCGCGGGCCA
AAGGTGCCAGCCGTAGCGGGGTGCTATGGCGGCATGTGGCGCGTAACGCCATGCTGCCTGCGCTGACCATCGCCGGCATT
CTGCTGGGTGAGTTGATCGCCGGGGCGCTGATCACCGAAACCGTATTTGGCCGTAGCGGGCTGGGACAACTCACCTTACA
GGCAGTACTGAACCAGGATGTGGCGGTGTTGCAGGCGATAGTCGTCATTTCCGCCGCCGCTTTCGTCACTCTCAACCTGC
TGGTCGATCTGCTGTTCCCACTGTTGGATCCACGGCTGAAAACCCATACAGGAGCCGCCGTATGA

Upstream 100 bases:

>100_bases
GTGTTTGCCGGTGCACCTTATCTGAAGGGGGTTAGTTTCGAAGCGGTCGGTCGCCCGAGTTTCTACGGCGCCTGGTTAGA
GAAACACTGAGGAGGCGATG

Downstream 100 bases:

>100_bases
GCAGTATTTCCTTTGAAAAGACCGTCGTGCCGGAGCGTGACCTGGGGGGCGTGGAAAGCGAAGGCACGTTATCGACCAGC
CGTTCACGTCGTCGACGCGT

Product: binding-protein-dependent transport systems inner membrane component

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSRYLARRIGQALLVLWATFSLSFLLLQVLPGDAILIKFQNPDMGLSPAQIADMRAAYGADVSLWQQYLHALGNVLRGDL
GYSIQAGVPVTELLATNLPATLQLAVLGFTLALLLALTIAFISNLTGFGWLRAALQSLPSLFVSVPTFWLGIVLIQVFSF
QLKLIPVINPGEWQGLILPIVTLAVPISAPLAQILIRSIDQVQTQPFVAVARAKGASRSGVLWRHVARNAMLPALTIAGI
LLGELIAGALITETVFGRSGLGQLTLQAVLNQDVAVLQAIVVISAAAFVTLNLLVDLLFPLLDPRLKTHTGAAV

Sequences:

>Translated_314_residues
MSRYLARRIGQALLVLWATFSLSFLLLQVLPGDAILIKFQNPDMGLSPAQIADMRAAYGADVSLWQQYLHALGNVLRGDL
GYSIQAGVPVTELLATNLPATLQLAVLGFTLALLLALTIAFISNLTGFGWLRAALQSLPSLFVSVPTFWLGIVLIQVFSF
QLKLIPVINPGEWQGLILPIVTLAVPISAPLAQILIRSIDQVQTQPFVAVARAKGASRSGVLWRHVARNAMLPALTIAGI
LLGELIAGALITETVFGRSGLGQLTLQAVLNQDVAVLQAIVVISAAAFVTLNLLVDLLFPLLDPRLKTHTGAAV
>Mature_313_residues
SRYLARRIGQALLVLWATFSLSFLLLQVLPGDAILIKFQNPDMGLSPAQIADMRAAYGADVSLWQQYLHALGNVLRGDLG
YSIQAGVPVTELLATNLPATLQLAVLGFTLALLLALTIAFISNLTGFGWLRAALQSLPSLFVSVPTFWLGIVLIQVFSFQ
LKLIPVINPGEWQGLILPIVTLAVPISAPLAQILIRSIDQVQTQPFVAVARAKGASRSGVLWRHVARNAMLPALTIAGIL
LGELIAGALITETVFGRSGLGQLTLQAVLNQDVAVLQAIVVISAAAFVTLNLLVDLLFPLLDPRLKTHTGAAV

Specific function: Probably part of the binding-protein-dependent transport system y4tOPQRS for a peptide. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=312, Percent_Identity=34.2948717948718, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787053, Length=309, Percent_Identity=31.7152103559871, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1789965, Length=338, Percent_Identity=30.1775147928994, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787497, Length=313, Percent_Identity=27.1565495207668, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787550, Length=273, Percent_Identity=27.1062271062271, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788504, Length=364, Percent_Identity=25.2747252747253, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787761, Length=337, Percent_Identity=29.080118694362, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33545; Mature: 33414

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRYLARRIGQALLVLWATFSLSFLLLQVLPGDAILIKFQNPDMGLSPAQIADMRAAYGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCC
DVSLWQQYLHALGNVLRGDLGYSIQAGVPVTELLATNLPATLQLAVLGFTLALLLALTIA
CHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
FISNLTGFGWLRAALQSLPSLFVSVPTFWLGIVLIQVFSFQLKLIPVINPGEWQGLILPI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHH
VTLAVPISAPLAQILIRSIDQVQTQPFVAVARAKGASRSGVLWRHVARNAMLPALTIAGI
HHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LLGELIAGALITETVFGRSGLGQLTLQAVLNQDVAVLQAIVVISAAAFVTLNLLVDLLFP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLDPRLKTHTGAAV
HHCCCHHCCCCCCC
>Mature Secondary Structure 
SRYLARRIGQALLVLWATFSLSFLLLQVLPGDAILIKFQNPDMGLSPAQIADMRAAYGA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCC
DVSLWQQYLHALGNVLRGDLGYSIQAGVPVTELLATNLPATLQLAVLGFTLALLLALTIA
CHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
FISNLTGFGWLRAALQSLPSLFVSVPTFWLGIVLIQVFSFQLKLIPVINPGEWQGLILPI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHH
VTLAVPISAPLAQILIRSIDQVQTQPFVAVARAKGASRSGVLWRHVARNAMLPALTIAGI
HHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LLGELIAGALITETVFGRSGLGQLTLQAVLNQDVAVLQAIVVISAAAFVTLNLLVDLLFP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLDPRLKTHTGAAV
HHCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9163424; 8796346 [H]