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The map label for this gene is gsiB [H]

Identifier: 157371396

GI number: 157371396

Start: 3475817

End: 3477442

Strand: Reverse

Name: gsiB [H]

Synonym: Spro_3157

Alternate gene names: 157371396

Gene position: 3477442-3475817 (Counterclockwise)

Preceding gene: 157371398

Following gene: 157371395

Centisome position: 63.82

GC content: 58.67

Gene sequence:

>1626_bases
ATGTTTAATTTCTCTTTCAATCGATTAACCAGCGGGATAACAGTGGCATTGGGACTGGCGGCGGCGATGAATCCGGCGCT
GGCGTCGGTGCAGGGCGGCACGCTTGTCTACCTGGAACAGCAGGCTCACACCAACCTTTATCCGCCTTCCGGCGGTTTCT
ACCCCAACGGCGGTATCCTCAATCAAATCACCGACAAACTGACCTACCAGAACCCGCAAACGCTGGAGATTGAGCCGTGG
ATTGCCGAGTCCTGGAGCAGCAACGCCGATAAAACCGAATACACCTTCAAGCTGCGGCCTGGCGTGACTTTCTCCGACGG
TACACCGCTGGATGCCAATGCAGTGGCGAAAAACTTCGATACCTATGGCCTGGGCAATAAAGAGAAACGTCTGCCGGTGT
CCGAAGTCATCAATAACTACGACCACAGCGAAGTGATCGACCCGTTAACCGTCAAGTTTTACTTCAAGCACTCGTCTCCC
GGCTTCCTGCAGGGCACCGCCACCATTGGTTCTGGCCTGGTTTCGCTCAGCACCCTGAACCGCAGCTATGACCAACTCGG
TGATGCGCGCCATATCATCGGTTCCGGCCCGTTTGTGGTCAGCGCCGAGACGCTGGGGCGTGAGGTCAGCCTGAGCGTTC
GCAAGGATTATCACTGGGGTCCGGCCAAATTGGCTCAGCAAGGGCGCGCTAATCTGGATGGCATCAAGGTGATTGTCACC
GGCGAAGACAGCGTGCGTATCGGTGCGTTGCAGGCTGGGCAGGCGGACTTTATTCGCCAGATCCAGGCCTACGACGAGAA
GCAGACGCAGGAACAGGGCTTTACGATTTACGCGGCCCCCACTCGTGGTGTCAACGACAGCGTCGCCTTCCGGCCGGATA
ACCCGCTGGTGAGCGACCTGCGCGTGCGTCAGGCACTGCTGCACGCCACCGACAGCAAGCAAATTGTCGATACGCTGTTC
TCGGTTAACTACCCACAGGCTAAATCGGTGATTGCTTCTTCCGCCGCCGGTTTCGTCGACTTATCCGCCAAGCTGAAATT
CGACCCGGAGCTGGCCAACCGTCTGCTGGATGAGGCGGGCTGGAAAAAGGGCGGCGACGGCCTGCGTGAGAAGGACGGCA
AAAAATTGCTGCTGAATGTCTATGAATCGCTGCCGCAGCCGCAGAACAAGGCGGTGCTGCAGCTGGTTTCGCAGCAGTGG
GGCAAGGTCGGCGCGCGCTTGAACATTCTGGCGGGCGACGCCGGCAGCAAGGTGGCGGATAACCTCGATCCGCAGAAAAC
CCCGGCGGCGGTGGTGGAGGTGGGACGGGCGGATCCGGACGTGATTAAGAGCCAGTTCTACCCGACCAACCGCGATGCGC
TGTTGCAGCAGGGCGGGACGGGCAAAAACAGCGCATTCAAAGATGACAAGCTGAACGCGCTGCTGCTGGGCATCGCCTCT
GAGGTGGACCCGAAAAAACGCCTGCAGATTGCCGGTGAGGCGCAGAATTACCTGCTTGATCAGGCCTATGTGATCCCGTT
CTTCGAGGAGCCGCAGGTGTTTGCCGGTGCACCTTATCTGAAGGGGGTTAGTTTCGAAGCGGTCGGTCGCCCGAGTTTCT
ACGGCGCCTGGTTAGAGAAACACTGA

Upstream 100 bases:

>100_bases
TTTCCGCCATTGGTTAGCGAAATTAGTTCGTTCAACTCCCCGCCGATTTGGTTTGTTATCTAACCCATAACATTCCGAAA
TAAAGAAGGCGTGGCACACA

Downstream 100 bases:

>100_bases
GGAGGCGATGATGAGCCGATATCTGGCGCGGCGCATCGGGCAGGCGCTGCTGGTGCTGTGGGCGACCTTTAGCCTGTCGT
TTCTTCTGCTGCAGGTGCTG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MFNFSFNRLTSGITVALGLAAAMNPALASVQGGTLVYLEQQAHTNLYPPSGGFYPNGGILNQITDKLTYQNPQTLEIEPW
IAESWSSNADKTEYTFKLRPGVTFSDGTPLDANAVAKNFDTYGLGNKEKRLPVSEVINNYDHSEVIDPLTVKFYFKHSSP
GFLQGTATIGSGLVSLSTLNRSYDQLGDARHIIGSGPFVVSAETLGREVSLSVRKDYHWGPAKLAQQGRANLDGIKVIVT
GEDSVRIGALQAGQADFIRQIQAYDEKQTQEQGFTIYAAPTRGVNDSVAFRPDNPLVSDLRVRQALLHATDSKQIVDTLF
SVNYPQAKSVIASSAAGFVDLSAKLKFDPELANRLLDEAGWKKGGDGLREKDGKKLLLNVYESLPQPQNKAVLQLVSQQW
GKVGARLNILAGDAGSKVADNLDPQKTPAAVVEVGRADPDVIKSQFYPTNRDALLQQGGTGKNSAFKDDKLNALLLGIAS
EVDPKKRLQIAGEAQNYLLDQAYVIPFFEEPQVFAGAPYLKGVSFEAVGRPSFYGAWLEKH

Sequences:

>Translated_541_residues
MFNFSFNRLTSGITVALGLAAAMNPALASVQGGTLVYLEQQAHTNLYPPSGGFYPNGGILNQITDKLTYQNPQTLEIEPW
IAESWSSNADKTEYTFKLRPGVTFSDGTPLDANAVAKNFDTYGLGNKEKRLPVSEVINNYDHSEVIDPLTVKFYFKHSSP
GFLQGTATIGSGLVSLSTLNRSYDQLGDARHIIGSGPFVVSAETLGREVSLSVRKDYHWGPAKLAQQGRANLDGIKVIVT
GEDSVRIGALQAGQADFIRQIQAYDEKQTQEQGFTIYAAPTRGVNDSVAFRPDNPLVSDLRVRQALLHATDSKQIVDTLF
SVNYPQAKSVIASSAAGFVDLSAKLKFDPELANRLLDEAGWKKGGDGLREKDGKKLLLNVYESLPQPQNKAVLQLVSQQW
GKVGARLNILAGDAGSKVADNLDPQKTPAAVVEVGRADPDVIKSQFYPTNRDALLQQGGTGKNSAFKDDKLNALLLGIAS
EVDPKKRLQIAGEAQNYLLDQAYVIPFFEEPQVFAGAPYLKGVSFEAVGRPSFYGAWLEKH
>Mature_541_residues
MFNFSFNRLTSGITVALGLAAAMNPALASVQGGTLVYLEQQAHTNLYPPSGGFYPNGGILNQITDKLTYQNPQTLEIEPW
IAESWSSNADKTEYTFKLRPGVTFSDGTPLDANAVAKNFDTYGLGNKEKRLPVSEVINNYDHSEVIDPLTVKFYFKHSSP
GFLQGTATIGSGLVSLSTLNRSYDQLGDARHIIGSGPFVVSAETLGREVSLSVRKDYHWGPAKLAQQGRANLDGIKVIVT
GEDSVRIGALQAGQADFIRQIQAYDEKQTQEQGFTIYAAPTRGVNDSVAFRPDNPLVSDLRVRQALLHATDSKQIVDTLF
SVNYPQAKSVIASSAAGFVDLSAKLKFDPELANRLLDEAGWKKGGDGLREKDGKKLLLNVYESLPQPQNKAVLQLVSQQW
GKVGARLNILAGDAGSKVADNLDPQKTPAAVVEVGRADPDVIKSQFYPTNRDALLQQGGTGKNSAFKDDKLNALLLGIAS
EVDPKKRLQIAGEAQNYLLDQAYVIPFFEEPQVFAGAPYLKGVSFEAVGRPSFYGAWLEKH

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=531, Percent_Identity=25.9887005649718, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1789887, Length=353, Percent_Identity=23.2294617563739, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789966, Length=521, Percent_Identity=23.2245681381958, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1787762, Length=474, Percent_Identity=22.5738396624473, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58800; Mature: 58800

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNFSFNRLTSGITVALGLAAAMNPALASVQGGTLVYLEQQAHTNLYPPSGGFYPNGGIL
CCCCCHHHHHCCHHHHHHHHHHCCHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHH
NQITDKLTYQNPQTLEIEPWIAESWSSNADKTEYTFKLRPGVTFSDGTPLDANAVAKNFD
HHHHHHHHCCCCCEEEECCCHHHCCCCCCCCCEEEEEECCCEEECCCCCCCHHHHHHCCC
TYGLGNKEKRLPVSEVINNYDHSEVIDPLTVKFYFKHSSPGFLQGTATIGSGLVSLSTLN
CCCCCCCCCCCCHHHHHCCCCCHHHCCCEEEEEEEEECCCCEEEEHHHHHCCHHHHHHHH
RSYDQLGDARHIIGSGPFVVSAETLGREVSLSVRKDYHWGPAKLAQQGRANLDGIKVIVT
HHHHHHCCCCEEECCCCEEEEHHHCCCEEEEEEECCCCCCHHHHHHCCCCCCCCEEEEEE
GEDSVRIGALQAGQADFIRQIQAYDEKQTQEQGFTIYAAPTRGVNDSVAFRPDNPLVSDL
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEECCCCCCHHHH
RVRQALLHATDSKQIVDTLFSVNYPQAKSVIASSAAGFVDLSAKLKFDPELANRLLDEAG
HHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEECHHHHHHHHHHHC
WKKGGDGLREKDGKKLLLNVYESLPQPQNKAVLQLVSQQWGKVGARLNILAGDAGSKVAD
CCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHC
NLDPQKTPAAVVEVGRADPDVIKSQFYPTNRDALLQQGGTGKNSAFKDDKLNALLLGIAS
CCCCCCCCHHHHEECCCCHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEEHHC
EVDPKKRLQIAGEAQNYLLDQAYVIPFFEEPQVFAGAPYLKGVSFEAVGRPSFYGAWLEK
CCCHHHHEEECCCHHHHHHCCEEEEEECCCCCEEECCCCCCCCCEEECCCCCCCCHHHCC
H
C
>Mature Secondary Structure
MFNFSFNRLTSGITVALGLAAAMNPALASVQGGTLVYLEQQAHTNLYPPSGGFYPNGGIL
CCCCCHHHHHCCHHHHHHHHHHCCHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHH
NQITDKLTYQNPQTLEIEPWIAESWSSNADKTEYTFKLRPGVTFSDGTPLDANAVAKNFD
HHHHHHHHCCCCCEEEECCCHHHCCCCCCCCCEEEEEECCCEEECCCCCCCHHHHHHCCC
TYGLGNKEKRLPVSEVINNYDHSEVIDPLTVKFYFKHSSPGFLQGTATIGSGLVSLSTLN
CCCCCCCCCCCCHHHHHCCCCCHHHCCCEEEEEEEEECCCCEEEEHHHHHCCHHHHHHHH
RSYDQLGDARHIIGSGPFVVSAETLGREVSLSVRKDYHWGPAKLAQQGRANLDGIKVIVT
HHHHHHCCCCEEECCCCEEEEHHHCCCEEEEEEECCCCCCHHHHHHCCCCCCCCEEEEEE
GEDSVRIGALQAGQADFIRQIQAYDEKQTQEQGFTIYAAPTRGVNDSVAFRPDNPLVSDL
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEECCCCCCHHHH
RVRQALLHATDSKQIVDTLFSVNYPQAKSVIASSAAGFVDLSAKLKFDPELANRLLDEAG
HHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEECHHHHHHHHHHHC
WKKGGDGLREKDGKKLLLNVYESLPQPQNKAVLQLVSQQWGKVGARLNILAGDAGSKVAD
CCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHC
NLDPQKTPAAVVEVGRADPDVIKSQFYPTNRDALLQQGGTGKNSAFKDDKLNALLLGIAS
CCCCCCCCHHHHEECCCCHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEEHHC
EVDPKKRLQIAGEAQNYLLDQAYVIPFFEEPQVFAGAPYLKGVSFEAVGRPSFYGAWLEK
CCCHHHHEEECCCHHHHHHCCEEEEEECCCCCEEECCCCCCCCCEEECCCCCCCCHHHCC
H
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA