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The map label for this gene is melA [H]

Identifier: 157371342

GI number: 157371342

Start: 3414186

End: 3415493

Strand: Reverse

Name: melA [H]

Synonym: Spro_3103

Alternate gene names: 157371342

Gene position: 3415493-3414186 (Counterclockwise)

Preceding gene: 157371345

Following gene: 157371341

Centisome position: 62.68

GC content: 52.29

Gene sequence:

>1308_bases
ATGATTAAAGTAACTTTTATGGGCGCAGGCAGCACCATCTTCGCCAAGAACGTACTCGGCGATATCATGGCAACGCCGGC
ATTGAAAGAGGTGGATATCGCGCTGTACGACATTGATAGCGCCCGTCTCAATGAATCTTTCGCCATGCTCAGCAATATCA
ATCGCAATATTAACGCTGGCAGGGCGAAGATCACTCCCTACCTCGGGGTGGAAAATCGTCGTTCGGCGTTGAAAAATGCC
AATTATGTGGTTAACGCCATCCAGGTTGGCGGTTACGATCCTTGCACTATCACCGATTTCACCATTGCTAAAAAGTATGG
CCTGCAGCAAACCATTGCCGATACCCTGGGGATAGGTGGCATCTTCCGTGCGCTGCGCACCATTCCGGTGATGTTTGACT
TCGCCCGGGATATTGAAGCGGTATGTCCGGATGCCTGGTTGCTGAACTACACCAACCCGATGGCGGCCTTAACCGGCGCC
ATGCTGCGCCATACCGAAGTGAAAACGGTGGGGTTGTGTCACAGTGTGCAGGTCTGTGCAGAGACGCTGCTGAAAAGCGT
GGATATGCCTACCGATGATGTCCAGTTCCACATCGCAGGCATTAACCATATGGCCTGGTTGCTGGACGTTCGTCGCCATG
GCGAGGATCTGTACCCGGAAATCAAGCGTCGCGCCAATGCGCTGCAGGGCAAACATGATGATATGGTGCGCCATGAAATC
ATGAAAACCTTTGGCTATTACGTTACCGAGTCTTCGGAACATAACGCCGAGTACATGCCTTATTGGATCAAGCGTAACTA
TCCTGAATTGATTGAGCGCTTTAACATTCCGCTGGACGAGTACCCGCGCCGCTGTGTTGAGCAGATTGAACAATGGCAAC
AGCGCAAGCTGGCGCTGACTAATGACGCCAACCTGACTCATACCCGCACTCATGAGTATGCGTCTTATATTATTGAAGCG
ATGGAAACCGATCGCCCGTACAAGATTGGTGGCAATGTGCTCAACAGCGGTTTAATTACCAACCTGCCTGCCGAGGCCTG
TGTTGAAGTGCCTTGCCTGGTGGATGGGCAGGGCATCTCGCCCTGTTACGTCGGTCATTTACCGGAGCAACTGGCGGCGC
TCAACCGCACCAATATCAATACCCAACTTCTGACTATCGAAGCCGCAGTAACCCATAAACGCGAAGCGATTTACCACGCG
GCACTGCTGGATCCGCATACATCTGCCGAGCTTTCGATTGATGATATCCGTAAACTCTGCGATGAACTGATTGAGGCCCA
CGGTAACTGGCTTCCCGCCTACCACTGA

Upstream 100 bases:

>100_bases
CGTCGTTTTTCGCCATAATGAGGTCACATTCATCCATATCCGGCTTAGGGATTGCAGGGTAATTTCAGCCTATCGAAAAC
CAGTGCAGCGGAGAAATAGG

Downstream 100 bases:

>100_bases
TCGCCAAACTAACTGTATTACCTCCTCAAAACGCACAAGGCGCGGGGTTTCCTGCGCCGTCATAAATGGGATGGTGAGCC
ATGTTTTATTTAAAGAACAA

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MIKVTFMGAGSTIFAKNVLGDIMATPALKEVDIALYDIDSARLNESFAMLSNINRNINAGRAKITPYLGVENRRSALKNA
NYVVNAIQVGGYDPCTITDFTIAKKYGLQQTIADTLGIGGIFRALRTIPVMFDFARDIEAVCPDAWLLNYTNPMAALTGA
MLRHTEVKTVGLCHSVQVCAETLLKSVDMPTDDVQFHIAGINHMAWLLDVRRHGEDLYPEIKRRANALQGKHDDMVRHEI
MKTFGYYVTESSEHNAEYMPYWIKRNYPELIERFNIPLDEYPRRCVEQIEQWQQRKLALTNDANLTHTRTHEYASYIIEA
METDRPYKIGGNVLNSGLITNLPAEACVEVPCLVDGQGISPCYVGHLPEQLAALNRTNINTQLLTIEAAVTHKREAIYHA
ALLDPHTSAELSIDDIRKLCDELIEAHGNWLPAYH

Sequences:

>Translated_435_residues
MIKVTFMGAGSTIFAKNVLGDIMATPALKEVDIALYDIDSARLNESFAMLSNINRNINAGRAKITPYLGVENRRSALKNA
NYVVNAIQVGGYDPCTITDFTIAKKYGLQQTIADTLGIGGIFRALRTIPVMFDFARDIEAVCPDAWLLNYTNPMAALTGA
MLRHTEVKTVGLCHSVQVCAETLLKSVDMPTDDVQFHIAGINHMAWLLDVRRHGEDLYPEIKRRANALQGKHDDMVRHEI
MKTFGYYVTESSEHNAEYMPYWIKRNYPELIERFNIPLDEYPRRCVEQIEQWQQRKLALTNDANLTHTRTHEYASYIIEA
METDRPYKIGGNVLNSGLITNLPAEACVEVPCLVDGQGISPCYVGHLPEQLAALNRTNINTQLLTIEAAVTHKREAIYHA
ALLDPHTSAELSIDDIRKLCDELIEAHGNWLPAYH
>Mature_435_residues
MIKVTFMGAGSTIFAKNVLGDIMATPALKEVDIALYDIDSARLNESFAMLSNINRNINAGRAKITPYLGVENRRSALKNA
NYVVNAIQVGGYDPCTITDFTIAKKYGLQQTIADTLGIGGIFRALRTIPVMFDFARDIEAVCPDAWLLNYTNPMAALTGA
MLRHTEVKTVGLCHSVQVCAETLLKSVDMPTDDVQFHIAGINHMAWLLDVRRHGEDLYPEIKRRANALQGKHDDMVRHEI
MKTFGYYVTESSEHNAEYMPYWIKRNYPELIERFNIPLDEYPRRCVEQIEQWQQRKLALTNDANLTHTRTHEYASYIIEA
METDRPYKIGGNVLNSGLITNLPAEACVEVPCLVDGQGISPCYVGHLPEQLAALNRTNINTQLLTIEAAVTHKREAIYHA
ALLDPHTSAELSIDDIRKLCDELIEAHGNWLPAYH

Specific function: Unknown

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=447, Percent_Identity=49.2170022371365, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI1788029, Length=455, Percent_Identity=25.4945054945055, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 48903; Mature: 48903

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVTFMGAGSTIFAKNVLGDIMATPALKEVDIALYDIDSARLNESFAMLSNINRNINAG
CEEEEEECCCCHHHHHHHHHHHHCCCCHHHCEEEEEECCHHHHHHHHHHHHHCCCCCCCC
RAKITPYLGVENRRSALKNANYVVNAIQVGGYDPCTITDFTIAKKYGLQQTIADTLGIGG
CEEECEECCCCHHHHHHCCCCEEEEEEEECCCCCCEEEEEHHHHHHCHHHHHHHHHCHHH
IFRALRTIPVMFDFARDIEAVCPDAWLLNYTNPMAALTGAMLRHTEVKTVGLCHSVQVCA
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ETLLKSVDMPTDDVQFHIAGINHMAWLLDVRRHGEDLYPEIKRRANALQGKHDDMVRHEI
HHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHH
MKTFGYYVTESSEHNAEYMPYWIKRNYPELIERFNIPLDEYPRRCVEQIEQWQQRKLALT
HHHHHHEEECCCCCCCCCCCHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCEEEE
NDANLTHTRTHEYASYIIEAMETDRPYKIGGNVLNSGLITNLPAEACVEVPCLVDGQGIS
CCCCCCCCHHHHHHHHHHHHHHCCCCEECCCHHHCCCCCCCCCHHHHHCCCEEECCCCCC
PCYVGHLPEQLAALNRTNINTQLLTIEAAVTHKREAIYHAALLDPHTSAELSIDDIRKLC
CHHHCCCHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
DELIEAHGNWLPAYH
HHHHHHCCCCCCCCC
>Mature Secondary Structure
MIKVTFMGAGSTIFAKNVLGDIMATPALKEVDIALYDIDSARLNESFAMLSNINRNINAG
CEEEEEECCCCHHHHHHHHHHHHCCCCHHHCEEEEEECCHHHHHHHHHHHHHCCCCCCCC
RAKITPYLGVENRRSALKNANYVVNAIQVGGYDPCTITDFTIAKKYGLQQTIADTLGIGG
CEEECEECCCCHHHHHHCCCCEEEEEEEECCCCCCEEEEEHHHHHHCHHHHHHHHHCHHH
IFRALRTIPVMFDFARDIEAVCPDAWLLNYTNPMAALTGAMLRHTEVKTVGLCHSVQVCA
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
ETLLKSVDMPTDDVQFHIAGINHMAWLLDVRRHGEDLYPEIKRRANALQGKHDDMVRHEI
HHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHH
MKTFGYYVTESSEHNAEYMPYWIKRNYPELIERFNIPLDEYPRRCVEQIEQWQQRKLALT
HHHHHHEEECCCCCCCCCCCHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCEEEE
NDANLTHTRTHEYASYIIEAMETDRPYKIGGNVLNSGLITNLPAEACVEVPCLVDGQGIS
CCCCCCCCHHHHHHHHHHHHHHCCCCEECCCHHHCCCCCCCCCHHHHHCCCEEECCCCCC
PCYVGHLPEQLAALNRTNINTQLLTIEAAVTHKREAIYHAALLDPHTSAELSIDDIRKLC
CHHHCCCHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
DELIEAHGNWLPAYH
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]