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The map label for this gene is lacY [H]

Identifier: 157371341

GI number: 157371341

Start: 3412849

End: 3414105

Strand: Reverse

Name: lacY [H]

Synonym: Spro_3102

Alternate gene names: 157371341

Gene position: 3414105-3412849 (Counterclockwise)

Preceding gene: 157371342

Following gene: 157371337

Centisome position: 62.66

GC content: 52.43

Gene sequence:

>1257_bases
ATGTTTTATTTAAAGAACAAGAACTTTTGGATCTTCGGTGCCTTCTGTTTTTTCTATTTCTTTATCATGGGGGCGGTGCT
GCCGTTCTTCCCGATCTGGTTGCATGACGTCAACCAACTCGACCAGCAACAAACCGGTCTGGTATTCGCCTGCATGGCGC
TGTTTGCGTTGGTGTTCCAGCCGATTTTTGGTTTTGTCACCGACAAATTTGGCCTGAAGAAACACCTGTTGTGGATGATC
ATTTTCCTGCTGCTGTTTCTGGCACCGCTGTTTATTTATGTCTTCTCGCCGCTGCTGCAAACCAACTTTGTGCTCGGGGC
GCTATTGTGCGGGATTTACCTGGGGTTGGTGTGCAGCGGAGGCTCGCCGGCAATTGAAGCCTATATCGAGAAGGTGAGCC
GCCGCAGCCAGTTTGAGTATGGTCGTGCCCGGCTGTTTGGCTGTATCGGCTGGGCGATTTGCGCGTCGATCGTTGGCTAT
ATGTTTACCATCAACAACCAGTTTGCGTTTTGGCTGGCGTCGAGCTTTGCACTGGTGTTGGCCGGCCTGCTTTATATGTT
CAAACCTGAGCAAAACAACACGCTGGCGGTGGCGGACGGCTTGGCGCTGAACCACAAACCGGTCAAGTTGAAAGCGGCGT
TGAGCCTGCTGAAAATGCGCAAATTTTGGTGCCTGGTGATGTATATCGTCGGGGTGGCCTGCGTGTACGACGTGTTCGAT
CAGCAGTTCGCCAACTTCTTCACTTCGTTTTTCAGCGACCGACATGCCGGGACTCAGGCGTTTGGCTATGTCACCACGCT
GGGCGAGTTCCTCAACGCTTTTATCATGTTCTTTGCGCCATTGATCATCAACCGCATTGGCGGCAAGAACGCGCTGCTGA
TTGCCGGGGTGATTATGTCGGTGCGGATTATCGGTTCGTCTCAGGCTGACTCGGTGACTGCGGTGATTTTCCTCAAAACC
CTGCATATGTTTGAGGTGCCGTTCCTGCTGGTGGGCATTTTCAAATACATCACCACCCAGTTTAATGTGAATCTTTCCGC
ATCGATTTACCTGTGGGGTTTCTGCTTCTTCAAGCAGCTATCGGCTATCGGCATGTCCTATGCGGCGGGCCTGATGTACG
TCAACTACGGCTTTAAAACCTCGTATCTGATCCTCGGCTGCATTGCCTTGCTGTTTACCCTGATCTCGGCCTTTGCCCTG
AGCGGCAAGGTCGCGGTGGTAAAACAGGAGGCTGTGCTGCCGGTGGCGGCGAATTAA

Upstream 100 bases:

>100_bases
GGCTTCCCGCCTACCACTGATCGCCAAACTAACTGTATTACCTCCTCAAAACGCACAAGGCGCGGGGTTTCCTGCGCCGT
CATAAATGGGATGGTGAGCC

Downstream 100 bases:

>100_bases
TCAATACAGTAATGTCATGAGGCTAAAGAACAGCAATGTCAGGGCGATGATAAACCCGGTTTGCAGCGTTTTGCGCAGCA
GGCGGCCCTGGCCCCAGCGG

Product: major facilitator superfamily oligosaccharide/H+ symporter

Products: Proton [Cytoplasm]; lactose [Cytoplasm] [C]

Alternate protein names: Lactose-proton symport [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MFYLKNKNFWIFGAFCFFYFFIMGAVLPFFPIWLHDVNQLDQQQTGLVFACMALFALVFQPIFGFVTDKFGLKKHLLWMI
IFLLLFLAPLFIYVFSPLLQTNFVLGALLCGIYLGLVCSGGSPAIEAYIEKVSRRSQFEYGRARLFGCIGWAICASIVGY
MFTINNQFAFWLASSFALVLAGLLYMFKPEQNNTLAVADGLALNHKPVKLKAALSLLKMRKFWCLVMYIVGVACVYDVFD
QQFANFFTSFFSDRHAGTQAFGYVTTLGEFLNAFIMFFAPLIINRIGGKNALLIAGVIMSVRIIGSSQADSVTAVIFLKT
LHMFEVPFLLVGIFKYITTQFNVNLSASIYLWGFCFFKQLSAIGMSYAAGLMYVNYGFKTSYLILGCIALLFTLISAFAL
SGKVAVVKQEAVLPVAAN

Sequences:

>Translated_418_residues
MFYLKNKNFWIFGAFCFFYFFIMGAVLPFFPIWLHDVNQLDQQQTGLVFACMALFALVFQPIFGFVTDKFGLKKHLLWMI
IFLLLFLAPLFIYVFSPLLQTNFVLGALLCGIYLGLVCSGGSPAIEAYIEKVSRRSQFEYGRARLFGCIGWAICASIVGY
MFTINNQFAFWLASSFALVLAGLLYMFKPEQNNTLAVADGLALNHKPVKLKAALSLLKMRKFWCLVMYIVGVACVYDVFD
QQFANFFTSFFSDRHAGTQAFGYVTTLGEFLNAFIMFFAPLIINRIGGKNALLIAGVIMSVRIIGSSQADSVTAVIFLKT
LHMFEVPFLLVGIFKYITTQFNVNLSASIYLWGFCFFKQLSAIGMSYAAGLMYVNYGFKTSYLILGCIALLFTLISAFAL
SGKVAVVKQEAVLPVAAN
>Mature_418_residues
MFYLKNKNFWIFGAFCFFYFFIMGAVLPFFPIWLHDVNQLDQQQTGLVFACMALFALVFQPIFGFVTDKFGLKKHLLWMI
IFLLLFLAPLFIYVFSPLLQTNFVLGALLCGIYLGLVCSGGSPAIEAYIEKVSRRSQFEYGRARLFGCIGWAICASIVGY
MFTINNQFAFWLASSFALVLAGLLYMFKPEQNNTLAVADGLALNHKPVKLKAALSLLKMRKFWCLVMYIVGVACVYDVFD
QQFANFFTSFFSDRHAGTQAFGYVTTLGEFLNAFIMFFAPLIINRIGGKNALLIAGVIMSVRIIGSSQADSVTAVIFLKT
LHMFEVPFLLVGIFKYITTQFNVNLSASIYLWGFCFFKQLSAIGMSYAAGLMYVNYGFKTSYLILGCIALLFTLISAFAL
SGKVAVVKQEAVLPVAAN

Specific function: Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family [H]

Homologues:

Organism=Escherichia coli, GI1786538, Length=402, Percent_Identity=63.681592039801, Blast_Score=498, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022814
- InterPro:   IPR000576
- InterPro:   IPR018457
- InterPro:   IPR020846
- InterPro:   IPR016196 [H]

Pfam domain/function: PF01306 LacY_symp [H]

EC number: NA

Molecular weight: Translated: 46795; Mature: 46795

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS50850 MFS ; PS00897 LACY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; lactose [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactose [Periplasm] = Proton [Cytoplasm] + lactose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6444453; 9278503; 2164211; 7578103; 1848449; 1644770; 2203471; 10485888; 12893935 [H]