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The map label for this gene is nikA [H]

Identifier: 157370981

GI number: 157370981

Start: 3019136

End: 3020644

Strand: Direct

Name: nikA [H]

Synonym: Spro_2741

Alternate gene names: 157370981

Gene position: 3019136-3020644 (Clockwise)

Preceding gene: 157370980

Following gene: 157370982

Centisome position: 55.41

GC content: 60.37

Gene sequence:

>1509_bases
ATGTTGAACCCACGCTTTATTGGCCCCACCGGCTGCCTGCTCGCCGGCAGCTTGCTGCTTGGCGGCTGTTTTGACCCTGC
CGAACCGAAAGCAGGTAACGGCGAAGAACGGCGCATCCGGCTGGCCATGTTGCAACCGCCTCGTTCCGGGCTCACGCCGC
TCAGCGATGATGCGTTTAAACTTTCGCGCTGGAGCAATACCGAAACGCTGGTGCTGCTGAACGCCAATGGTGATGCCGAA
CCGGCATTGGCCACCGAATGGCAGCAGATAGATCAACACCGCTGGCGCTTTGTGTTGCGGCAGGGGGTCAGTTTCCACGA
CGGCAGTGTGATGACAGCACAGAGCGTCGTACGCTCACTTTCGGCGGCAATCAACGCCGCACCCAAACCGCGTATCCTCG
ACGGCATTGAATTATCAGTCAGCATTGAAGGCGATCACGCCGTGATTATTTCCACCGCGGTCCCCGACCCTTTACTTCCG
CAACGGCTGTCCAGCCCACAGCTGGCGATCCTTGCCGAACGGGCTTACGGTGCCAACGGCGTGGTTAACCCATTGCTGGC
CGGGACCGGCGCTTTTGTGCTGACGGAAATCAACGGGACCAGCAGCGCGCGACTAAAGCGTTTTGAAGGTTATTGGGGCC
AAAAAGCCGCAGCGCCGGGTATCGACGTCAGTTTTGTACCGGACGGTTCCGCCCGAGCCGCAGCTCTGCGCACCGGCAGT
GCCGATCTGGTTGAAGCCGTGCCGGTATCACAGTTGCCACTGATTGCGGCAGAACGCATCCATGAAGTGCCGATGCCGCG
CACCAATACCCTGTATCTGAACACCCGAGTAGGTGTCTTTAGCGATCCGCAGGTGCGTGCCGCCGCCGCCGCGGCTATTG
AGCGTCAACCCCTGGTGGACAATGTCTACGAAGGCCGTGCCGATGTCGCCAGCGGGCTGCTTGGCCCGGCACTGCCCTGG
GCTGCCCCATTGAGACAGCCGCAACCCTTGCTGCCCACCAAGACGGTAACGAATAAAACCATCACCCTGGCCACCTTCAG
CGATCGCGCCGAGCTACCGGAAGTCGCCGTTTATCTGGCGCAACAGCTTGGCGCCGCAGGCTTTGTGGTGAAACAGGAAG
TGCGGGAATATGCGCATATTGAGGCCGATGCGCTGGCAGGCAAATTCGACGCATTTATTCTGTCGCGAGCCACGGTGTTG
GACTCCGGCGATCCACTGGCTTATATGTTCAGCGATTTCGCCTGTCAGGGCTCGTTCAATATCGCCCAACTGTGCGACCC
GCAGGTGGATGCGGCTATAACTCAGGCTGCCGCTATTGCGGCCGGAGCACAGCGGCGTCAGGCGATCATGGTGGCGGAAA
ATCGCATACTGGCCACTAATGCCGCGATCCCGCTGCTGCATGAACGAGTGATCCAGGGGGAAGCCGTGGGCATGAAGAAT
GCAGAGCGCGATCCGCGAGAACGCCAACTGGTGACACCGCGCAGCATGATCGTCAGCGGACAGCCCTGA

Upstream 100 bases:

>100_bases
ACTGCTGCGCAGCCCGCGACATGCCGCCACCTGCGATCTGCTGGCCGCCATTCCCTCGTTACCCGCCCTTTATCAGCCCT
GACATTCTGAGGACTTTTTC

Downstream 100 bases:

>100_bases
TGCGACTGGCGCAATGGCTATTGCCACTGGCCTCGCGGCTGTTTGCCCTGTTGGCAATGATATCCCTGATCGGTCTGATG
CCCTGGCTATCCGGTCGCGA

Product: extracellular solute-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MLNPRFIGPTGCLLAGSLLLGGCFDPAEPKAGNGEERRIRLAMLQPPRSGLTPLSDDAFKLSRWSNTETLVLLNANGDAE
PALATEWQQIDQHRWRFVLRQGVSFHDGSVMTAQSVVRSLSAAINAAPKPRILDGIELSVSIEGDHAVIISTAVPDPLLP
QRLSSPQLAILAERAYGANGVVNPLLAGTGAFVLTEINGTSSARLKRFEGYWGQKAAAPGIDVSFVPDGSARAAALRTGS
ADLVEAVPVSQLPLIAAERIHEVPMPRTNTLYLNTRVGVFSDPQVRAAAAAAIERQPLVDNVYEGRADVASGLLGPALPW
AAPLRQPQPLLPTKTVTNKTITLATFSDRAELPEVAVYLAQQLGAAGFVVKQEVREYAHIEADALAGKFDAFILSRATVL
DSGDPLAYMFSDFACQGSFNIAQLCDPQVDAAITQAAAIAAGAQRRQAIMVAENRILATNAAIPLLHERVIQGEAVGMKN
AERDPRERQLVTPRSMIVSGQP

Sequences:

>Translated_502_residues
MLNPRFIGPTGCLLAGSLLLGGCFDPAEPKAGNGEERRIRLAMLQPPRSGLTPLSDDAFKLSRWSNTETLVLLNANGDAE
PALATEWQQIDQHRWRFVLRQGVSFHDGSVMTAQSVVRSLSAAINAAPKPRILDGIELSVSIEGDHAVIISTAVPDPLLP
QRLSSPQLAILAERAYGANGVVNPLLAGTGAFVLTEINGTSSARLKRFEGYWGQKAAAPGIDVSFVPDGSARAAALRTGS
ADLVEAVPVSQLPLIAAERIHEVPMPRTNTLYLNTRVGVFSDPQVRAAAAAAIERQPLVDNVYEGRADVASGLLGPALPW
AAPLRQPQPLLPTKTVTNKTITLATFSDRAELPEVAVYLAQQLGAAGFVVKQEVREYAHIEADALAGKFDAFILSRATVL
DSGDPLAYMFSDFACQGSFNIAQLCDPQVDAAITQAAAIAAGAQRRQAIMVAENRILATNAAIPLLHERVIQGEAVGMKN
AERDPRERQLVTPRSMIVSGQP
>Mature_502_residues
MLNPRFIGPTGCLLAGSLLLGGCFDPAEPKAGNGEERRIRLAMLQPPRSGLTPLSDDAFKLSRWSNTETLVLLNANGDAE
PALATEWQQIDQHRWRFVLRQGVSFHDGSVMTAQSVVRSLSAAINAAPKPRILDGIELSVSIEGDHAVIISTAVPDPLLP
QRLSSPQLAILAERAYGANGVVNPLLAGTGAFVLTEINGTSSARLKRFEGYWGQKAAAPGIDVSFVPDGSARAAALRTGS
ADLVEAVPVSQLPLIAAERIHEVPMPRTNTLYLNTRVGVFSDPQVRAAAAAAIERQPLVDNVYEGRADVASGLLGPALPW
AAPLRQPQPLLPTKTVTNKTITLATFSDRAELPEVAVYLAQQLGAAGFVVKQEVREYAHIEADALAGKFDAFILSRATVL
DSGDPLAYMFSDFACQGSFNIAQLCDPQVDAAITQAAAIAAGAQRRQAIMVAENRILATNAAIPLLHERVIQGEAVGMKN
AERDPRERQLVTPRSMIVSGQP

Specific function: Involved in a nickel transport system, probably represents the nickel binder [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=289, Percent_Identity=28.0276816608997, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787052, Length=416, Percent_Identity=25.2403846153846, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787762, Length=418, Percent_Identity=22.9665071770335, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787551, Length=445, Percent_Identity=23.1460674157303, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1789966, Length=267, Percent_Identity=25.8426966292135, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1787495, Length=446, Percent_Identity=21.52466367713, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI87081878, Length=451, Percent_Identity=23.059866962306, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011980
- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 53511; Mature: 53511

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNPRFIGPTGCLLAGSLLLGGCFDPAEPKAGNGEERRIRLAMLQPPRSGLTPLSDDAFK
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEE
LSRWSNTETLVLLNANGDAEPALATEWQQIDQHRWRFVLRQGVSFHDGSVMTAQSVVRSL
HCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
SAAINAAPKPRILDGIELSVSIEGDHAVIISTAVPDPLLPQRLSSPQLAILAERAYGANG
HHHHCCCCCCCEECCEEEEEEECCCCEEEEEECCCCCCCHHHCCCCCEEEEEECCCCCCC
VVNPLLAGTGAFVLTEINGTSSARLKRFEGYWGQKAAAPGIDVSFVPDGSARAAALRTGS
CCCHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCEEEEECCC
ADLVEAVPVSQLPLIAAERIHEVPMPRTNTLYLNTRVGVFSDPQVRAAAAAAIERQPLVD
CHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCHH
NVYEGRADVASGLLGPALPWAAPLRQPQPLLPTKTVTNKTITLATFSDRAELPEVAVYLA
HHHCCHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHH
QQLGAAGFVVKQEVREYAHIEADALAGKFDAFILSRATVLDSGDPLAYMFSDFACQGSFN
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEHHHHHHCCCCCC
IAQLCDPQVDAAITQAAAIAAGAQRRQAIMVAENRILATNAAIPLLHERVIQGEAVGMKN
HHHHCCCHHHHHHHHHHHHHHHHHHCEEEEEECCEEEEECCCCHHHHHHHHCCCCCCCCC
AERDPRERQLVTPRSMIVSGQP
CCCCCHHHEECCCHHEEECCCC
>Mature Secondary Structure
MLNPRFIGPTGCLLAGSLLLGGCFDPAEPKAGNGEERRIRLAMLQPPRSGLTPLSDDAFK
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEE
LSRWSNTETLVLLNANGDAEPALATEWQQIDQHRWRFVLRQGVSFHDGSVMTAQSVVRSL
HCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
SAAINAAPKPRILDGIELSVSIEGDHAVIISTAVPDPLLPQRLSSPQLAILAERAYGANG
HHHHCCCCCCCEECCEEEEEEECCCCEEEEEECCCCCCCHHHCCCCCEEEEEECCCCCCC
VVNPLLAGTGAFVLTEINGTSSARLKRFEGYWGQKAAAPGIDVSFVPDGSARAAALRTGS
CCCHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCEEEEECCC
ADLVEAVPVSQLPLIAAERIHEVPMPRTNTLYLNTRVGVFSDPQVRAAAAAAIERQPLVD
CHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCHH
NVYEGRADVASGLLGPALPWAAPLRQPQPLLPTKTVTNKTITLATFSDRAELPEVAVYLA
HHHCCHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHH
QQLGAAGFVVKQEVREYAHIEADALAGKFDAFILSRATVLDSGDPLAYMFSDFACQGSFN
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEHHHHHHCCCCCC
IAQLCDPQVDAAITQAAAIAAGAQRRQAIMVAENRILATNAAIPLLHERVIQGEAVGMKN
HHHHCCCHHHHHHHHHHHHHHHHHHCEEEEEECCEEEEECCCCHHHHHHHHCCCCCCCCC
AERDPRERQLVTPRSMIVSGQP
CCCCCHHHEECCCHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]